Restriction map for
pF1KB5975
(Linear) (Six-base) MAPSORT of: KIBB5975.seq Check: 641 from: 1 to: 1557
KIBB5975.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 306 1557
Size: 306 1251
AccI GT'mk_AC
Cuts at: 0 569 1557
Size: 569 988
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 463 1403 1557
Size: 463 940 154
Fragments arranged by size:
940 463 154
AflIII A'CryG_T
Cuts at: 0 274 1557
Size: 274 1283
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 751 974 1557
Size: 751 223 583
Fragments arranged by size:
751 583 223
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 18 50 1557
Size: 18 32 1507
Fragments arranged by size:
1507 32 18
AlwNI CAG_nnn'CTG
Cuts at: 0 1383 1426 1453 1507 1557
Size: 1383 43 27 54 50
Fragments arranged by size:
1383 54 50 43 27
ApoI r'AATT_y
Cuts at: 0 1234 1557
Size: 1234 323
AvrII C'CTAG_G
Cuts at: 0 420 1557
Size: 420 1137
BanI G'GyrC_C
Cuts at: 0 897 1557
Size: 897 660
BanII G_rGCy'C
Cuts at: 0 1019 1390 1557
Size: 1019 371 167
Fragments arranged by size:
1019 371 167
BbsI GAAGACnn'nnnn_
Cuts at: 0 1409 1557
Size: 1409 148
BbvCI CC'TCA_GC
Cuts at: 0 5 1557
Size: 5 1552
BfrBI ATG'CAT
Cuts at: 0 961 1557
Size: 961 596
BglI GCCn_nnn'nGGC
Cuts at: 0 154 718 1557
Size: 154 564 839
Fragments arranged by size:
839 564 154
BglII A'GATC_T
Cuts at: 0 325 1557
Size: 325 1232
BmrI ACTGGGnnnn_n'
Cuts at: 0 1461 1557
Size: 1461 96
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1297 1557
Size: 1297 260
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 62 559 1267 1475 1516 1557
Size: 62 497 708 208 41 41
Fragments arranged by size:
708 497 208 62 41 41
Bpu10I CC'TnA_GC
Cuts at: 0 5 1557
Size: 5 1552
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 1198 1557
Size: 295 903 359
Fragments arranged by size:
903 359 295
BsaWI w'CCGG_w
Cuts at: 0 131 1557
Size: 131 1426
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 22 1557
Size: 22 1535
BseYI C'CCAG_C
Cuts at: 0 519 1557
Size: 519 1038
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 299 1557
Size: 299 1258
BsiHKAI G_wGCw'C
Cuts at: 0 1019 1557
Size: 1019 538
Bsp1286I G_dGCh'C
Cuts at: 0 1019 1390 1557
Size: 1019 371 167
Fragments arranged by size:
1019 371 167
BspEI T'CCGG_A
Cuts at: 0 131 1557
Size: 131 1426
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 306 1371 1557
Size: 306 1065 186
Fragments arranged by size:
1065 306 186
BsrDI GCAATG_nn'
Cuts at: 0 684 1418 1557
Size: 684 734 139
Fragments arranged by size:
734 684 139
BsrGI T'GTAC_A
Cuts at: 0 1145 1557
Size: 1145 412
BstXI CCAn_nnnn'nTGG
Cuts at: 0 894 1557
Size: 894 663
BstYI r'GATC_y
Cuts at: 0 325 527 1323 1557
Size: 325 202 796 234
Fragments arranged by size:
796 325 234 202
Bsu36I CC'TnA_GG
Cuts at: 0 1446 1557
Size: 1446 111
BtgI C'CryG_G
Cuts at: 0 744 1557
Size: 744 813
BtsI GCAGTG_nn'
Cuts at: 0 587 1040 1557
Size: 587 453 517
Fragments arranged by size:
587 517 453
EaeI y'GGCC_r
Cuts at: 0 320 863 986 1557
Size: 320 543 123 571
Fragments arranged by size:
571 543 320 123
EarI CTCTTCn'nnn_
Cuts at: 0 271 1493 1557
Size: 271 1222 64
Fragments arranged by size:
1222 271 64
EcoICRI GAG'CTC
Cuts at: 0 1017 1557
Size: 1017 540
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 62 463 559 1267 1403 1475 1516 1557
Size: 62 401 96 708 136 72 41 41
Fragments arranged by size:
708 401 136 96 72 62 41 41
EcoNI CCTnn'n_nnAGG
Cuts at: 0 313 1213 1557
Size: 313 900 344
Fragments arranged by size:
900 344 313
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 190 549 732 1557
Size: 190 359 183 825
Fragments arranged by size:
825 359 190 183
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 12 44 1210 1242 1315 1347 1557
Size: 12 32 1166 32 73 32 210
Fragments arranged by size:
1166 210 73 32 32 32 12
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1549 1557
Size: 1549 8
MscI TGG'CCA
Cuts at: 0 322 1557
Size: 322 1235
MslI CAynn'nnrTG
Cuts at: 0 829 1557
Size: 829 728
MspA1I CmG'CkG
Cuts at: 0 872 1426 1557
Size: 872 554 131
Fragments arranged by size:
872 554 131
NruI TCG'CGA
Cuts at: 0 19 1557
Size: 19 1538
NsiI A_TGCA'T
Cuts at: 0 963 1557
Size: 963 594
NspI r_CATG'y
Cuts at: 0 278 1026 1557
Size: 278 748 531
Fragments arranged by size:
748 531 278
PciI A'CATG_T
Cuts at: 0 274 1557
Size: 274 1283
PflMI CCAn_nnn'nTGG
Cuts at: 0 537 1557
Size: 537 1020
PvuII CAG'CTG
Cuts at: 0 872 1426 1557
Size: 872 554 131
Fragments arranged by size:
872 554 131
SacI G_AGCT'C
Cuts at: 0 1019 1557
Size: 1019 538
SmlI C'TyrA_G
Cuts at: 0 310 1213 1557
Size: 310 903 344
Fragments arranged by size:
903 344 310
StyI C'CwwG_G
Cuts at: 0 420 1557
Size: 420 1137
TatI w'GTAC_w
Cuts at: 0 1145 1557
Size: 1145 412
XmnI GAAnn'nnTTC
Cuts at: 0 173 448 1557
Size: 173 275 1109
Fragments arranged by size:
1109 275 173
Enzymes that do cut:
AarI AccI AcuI AflIII AlfI AloI AlwNI ApoI
AvrII BanI BanII BbsI BbvCI BfrBI BglI BglII
BmrI BplI BpmI Bpu10I BpuEI BsaWI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspEI BspMI BsrDI BsrGI BstXI
BstYI Bsu36I BtgI BtsI EaeI EarI EcoICRI Eco57MI
EcoNI FalI Hin4I MmeI MscI MslI MspA1I NruI
NsiI NspI PciI PflMI PvuII SacI SmlI StyI
TatI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
ApaI ApaLI AscI AseI AsiSI AvaI BaeI BamHI
BbeI BcgI BciVI BclI BlpI Bme1580I BmgBI BmtI
BsaI BsaAI BsaBI BsaHI BsaXI BsiEI BsiWI BsmI
BsmBI BspHI BsrBI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoO109I EcoRI EcoRV FseI FspI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
NaeI NarI NcoI NdeI NgoMIV NheI NotI PacI
PasI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TstI Tth111I XbaI XcmI XhoI
XmaI ZraI