Restriction map for
pF1KB5980
(Linear) (Six-base) MAPSORT of: KIBB5980.seq Check: 2236 from: 1 to: 1701
KIBB5980.orf
With 182 enzymes: SgfI *
November 27, 2008 10:48 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 570 1701
Size: 570 1131
Acc65I G'GTAC_C
Cuts at: 0 233 1701
Size: 233 1468
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 432 935 1701
Size: 432 503 766
Fragments arranged by size:
766 503 432
AfeI AGC'GCT
Cuts at: 0 307 1701
Size: 307 1394
AflIII A'CryG_T
Cuts at: 0 218 1701
Size: 218 1483
AgeI A'CCGG_T
Cuts at: 0 114 778 1701
Size: 114 664 923
Fragments arranged by size:
923 664 114
AleI CACnn'nnGTG
Cuts at: 0 115 1701
Size: 115 1586
AlwNI CAG_nnn'CTG
Cuts at: 0 129 546 585 873 1701
Size: 129 417 39 288 828
Fragments arranged by size:
828 417 288 129 39
ApaI G_GGCC'C
Cuts at: 0 80 1701
Size: 80 1621
ApaLI G'TGCA_C
Cuts at: 0 1246 1701
Size: 1246 455
AvaI C'yCGr_G
Cuts at: 0 79 87 643 964 1011 1496 1701
Size: 79 8 556 321 47 485 205
Fragments arranged by size:
556 485 321 205 79 47 8
BanI G'GyrC_C
Cuts at: 0 117 233 683 723 970 1156 1309 1701
Size: 117 116 450 40 247 186 153 392
Fragments arranged by size:
450 392 247 186 153 117 116 40
BanII G_rGCy'C
Cuts at: 0 80 94 148 1042 1190 1701
Size: 80 14 54 894 148 511
Fragments arranged by size:
894 511 148 80 54 14
BbeI G_GCGC'C
Cuts at: 0 687 727 974 1313 1701
Size: 687 40 247 339 388
Fragments arranged by size:
687 388 339 247 40
BbsI GAAGACnn'nnnn_
Cuts at: 0 173 1271 1412 1701
Size: 173 1098 141 289
Fragments arranged by size:
1098 289 173 141
BbvCI CC'TCA_GC
Cuts at: 0 902 1030 1701
Size: 902 128 671
Fragments arranged by size:
902 671 128
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1134 1168 1341 1375 1701
Size: 1134 34 173 34 326
Fragments arranged by size:
1134 326 173 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 143 886 898 1354 1701
Size: 143 743 12 456 347
Fragments arranged by size:
743 456 347 143 12
Bme1580I G_kGCm'C
Cuts at: 0 80 557 1250 1701
Size: 80 477 693 451
Fragments arranged by size:
693 477 451 80
BmgBI CAC'GTC
Cuts at: 0 219 1701
Size: 219 1482
BmrI ACTGGGnnnn_n'
Cuts at: 0 262 398 1560 1701
Size: 262 136 1162 141
Fragments arranged by size:
1162 262 141 136
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 511 865 895 1204 1359 1421 1663 1701
Size: 511 354 30 309 155 62 242 38
Fragments arranged by size:
511 354 309 242 155 62 38 30
Bpu10I CC'TnA_GC
Cuts at: 0 902 1030 1701
Size: 902 128 671
Fragments arranged by size:
902 671 128
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 936 1528 1701
Size: 936 592 173
Fragments arranged by size:
936 592 173
BsaI GGTCTCn'nnnn_
Cuts at: 0 415 1114 1701
Size: 415 699 587
Fragments arranged by size:
699 587 415
BsaAI yAC'GTr
Cuts at: 0 240 1701
Size: 240 1461
BsaBI GATnn'nnATC
Cuts at: 0 1210 1216 1701
Size: 1210 6 485
Fragments arranged by size:
1210 485 6
BsaHI Gr'CG_yC
Cuts at: 0 684 724 971 1310 1701
Size: 684 40 247 339 391
Fragments arranged by size:
684 391 339 247 40
BsaWI w'CCGG_w
Cuts at: 0 114 778 1511 1701
Size: 114 664 733 190
Fragments arranged by size:
733 664 190 114
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 461 491 1701
Size: 461 30 1210
Fragments arranged by size:
1210 461 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 502 862 1532 1701
Size: 502 360 670 169
Fragments arranged by size:
670 502 360 169
BseYI C'CCAG_C
Cuts at: 0 257 287 498 990 1241 1467 1701
Size: 257 30 211 492 251 226 234
Fragments arranged by size:
492 257 251 234 226 211 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 571 1701
Size: 571 1130
BsiEI CG_ry'CG
Cuts at: 0 1023 1701
Size: 1023 678
BsiHKAI G_wGCw'C
Cuts at: 0 1250 1345 1701
Size: 1250 95 356
Fragments arranged by size:
1250 356 95
BsmI GAATG_Cn'
Cuts at: 0 1395 1701
Size: 1395 306
Bsp1286I G_dGCh'C
Cuts at: 0 80 94 148 557 1042 1190 1250 1345
Size: 80 14 54 409 485 148 60 95
Cuts at: 1345 1701
Size: 356
Fragments arranged by size:
485 409 356 148 95 80 60 54
14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 570 872 970 1701
Size: 570 302 98 731
Fragments arranged by size:
731 570 302 98
BsrBI CCG'CTC
Cuts at: 0 740 852 1701
Size: 740 112 849
Fragments arranged by size:
849 740 112
BsrFI r'CCGG_y
Cuts at: 0 114 778 1701
Size: 114 664 923
Fragments arranged by size:
923 664 114
BstAPI GCAn_nnn'nTGC
Cuts at: 0 585 1238 1349 1701
Size: 585 653 111 352
Fragments arranged by size:
653 585 352 111
BstYI r'GATC_y
Cuts at: 0 452 1701
Size: 452 1249
Bsu36I CC'TnA_GG
Cuts at: 0 1050 1701
Size: 1050 651
BtgI C'CryG_G
Cuts at: 0 701 881 1701
Size: 701 180 820
Fragments arranged by size:
820 701 180
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1416 1701
Size: 1416 285
BtsI GCAGTG_nn'
Cuts at: 0 752 1701
Size: 752 949
DraIII CAC_nnn'GTG
Cuts at: 0 204 243 614 707 1443 1701
Size: 204 39 371 93 736 258
Fragments arranged by size:
736 371 258 204 93 39
EaeI y'GGCC_r
Cuts at: 0 107 1020 1367 1701
Size: 107 913 347 334
Fragments arranged by size:
913 347 334 107
EagI C'GGCC_G
Cuts at: 0 1020 1701
Size: 1020 681
EarI CTCTTCn'nnn_
Cuts at: 0 325 559 954 1002 1075 1701
Size: 325 234 395 48 73 626
Fragments arranged by size:
626 395 325 234 73 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 432 511 865 895 935 1204 1359 1421
Size: 432 79 354 30 40 269 155 62
Cuts at: 1421 1663 1701
Size: 242 38
Fragments arranged by size:
432 354 269 242 155 79 62 40
38 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1561 1597 1701
Size: 1561 36 104
Fragments arranged by size:
1561 104 36
EcoO109I rG'GnC_Cy
Cuts at: 0 135 543 861 982 1128 1554 1701
Size: 135 408 318 121 146 426 147
Fragments arranged by size:
426 408 318 147 146 135 121
FspI TGC'GCA
Cuts at: 0 247 1701
Size: 247 1454
HaeII r_GCGC'y
Cuts at: 0 8 309 687 727 974 1313 1619 1701
Size: 8 301 378 40 247 339 306 82
Fragments arranged by size:
378 339 306 301 247 82 40 8
KasI G'GCGC_C
Cuts at: 0 683 723 970 1309 1701
Size: 683 40 247 339 392
Fragments arranged by size:
683 392 339 247 40
KpnI G_GTAC'C
Cuts at: 0 237 1701
Size: 237 1464
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 173 557 1701
Size: 173 384 1144
Fragments arranged by size:
1144 384 173
MscI TGG'CCA
Cuts at: 0 109 1369 1701
Size: 109 1260 332
Fragments arranged by size:
1260 332 109
MslI CAynn'nnrTG
Cuts at: 0 115 1701
Size: 115 1586
MspA1I CmG'CkG
Cuts at: 0 32 41 358 935 1669 1701
Size: 32 9 317 577 734 32
Fragments arranged by size:
734 577 317 32 32 9
NarI GG'CG_CC
Cuts at: 0 684 724 971 1310 1701
Size: 684 40 247 339 391
Fragments arranged by size:
684 391 339 247 40
NcoI C'CATG_G
Cuts at: 0 881 1701
Size: 881 820
PflMI CCAn_nnn'nTGG
Cuts at: 0 278 707 1701
Size: 278 429 994
Fragments arranged by size:
994 429 278
PfoI T'CCnGG_A
Cuts at: 0 389 1701
Size: 389 1312
PmlI CAC'GTG
Cuts at: 0 240 1701
Size: 240 1461
PpuMI rG'GwC_Cy
Cuts at: 0 861 982 1701
Size: 861 121 719
Fragments arranged by size:
861 719 121
PshAI GACnn'nnGTC
Cuts at: 0 427 1412 1701
Size: 427 985 289
Fragments arranged by size:
985 427 289
PspOMI G'GGCC_C
Cuts at: 0 76 1701
Size: 76 1625
PstI C_TGCA'G
Cuts at: 0 134 869 1701
Size: 134 735 832
Fragments arranged by size:
832 735 134
PvuII CAG'CTG
Cuts at: 0 358 1701
Size: 358 1343
SapI GCTCTTCn'nnn_
Cuts at: 0 559 1701
Size: 559 1142
SfcI C'TryA_G
Cuts at: 0 130 865 1410 1701
Size: 130 735 545 291
Fragments arranged by size:
735 545 291 130
SfoI GGC'GCC
Cuts at: 0 685 725 972 1311 1701
Size: 685 40 247 339 390
Fragments arranged by size:
685 390 339 247 40
SgrAI Cr'CCGG_yG
Cuts at: 0 114 1701
Size: 114 1587
SmaI CCC'GGG
Cuts at: 0 966 1013 1498 1701
Size: 966 47 485 203
Fragments arranged by size:
966 485 203 47
SmlI C'TyrA_G
Cuts at: 0 915 1543 1701
Size: 915 628 158
Fragments arranged by size:
915 628 158
StuI AGG'CCT
Cuts at: 0 67 1701
Size: 67 1634
StyI C'CwwG_G
Cuts at: 0 881 1701
Size: 881 820
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 910 1233 1701
Size: 910 323 468
Fragments arranged by size:
910 468 323
TatI w'GTAC_w
Cuts at: 0 1640 1701
Size: 1640 61
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 461 493 1701
Size: 461 32 1208
Fragments arranged by size:
1208 461 32
XmaI C'CCGG_G
Cuts at: 0 964 1011 1496 1701
Size: 964 47 485 205
Fragments arranged by size:
964 485 205 47
Enzymes that do cut:
AarI Acc65I AcuI AfeI AflIII AgeI AleI AlwNI
ApaI ApaLI AvaI BanI BanII BbeI BbsI BbvCI
BcgI BglI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrFI
BstAPI BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI
EagI EarI Eco57MI EcoNI EcoO109I FspI HaeII KasI
KpnI MmeI MscI MslI MspA1I NarI NcoI PflMI
PfoI PmlI PpuMI PshAI PspOMI PstI PvuII SapI
SfcI SfoI SgrAI SmaI SmlI StuI StyI TaqII
TatI TstI XmaI
Enzymes that do not cut:
AatII AccI AclI AflII AhdI AlfI AloI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BciVI BclI
BfrBI BglII BlpI BmtI BplI BsiWI BsmBI BspEI
BspHI BsrDI BsrGI BssHII BssSI BstBI BstEII BstXI
BstZ17I ClaI CspCI DraI DrdI EciI EcoICRI EcoRI
EcoRV FalI FseI FspAI Hin4I HincII HindIII HpaI
MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PmeI PpiI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfiI SgfI SnaBI SpeI SphI
SrfI SspI SwaI Tth111I XbaI XcmI XhoI XmnI
ZraI