Restriction map for
pF1KB5982
(Linear) (Six-base) MAPSORT of: KIBB5982.seq Check: 3848 from: 1 to: 1704
KIBB5982.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 509 596 1704
Size: 322 187 87 1108
Fragments arranged by size:
1108 322 187 87
AfeI AGC'GCT
Cuts at: 0 1192 1669 1704
Size: 1192 477 35
Fragments arranged by size:
1192 477 35
AflIII A'CryG_T
Cuts at: 0 288 1704
Size: 288 1416
AleI CACnn'nnGTG
Cuts at: 0 437 1704
Size: 437 1267
ApoI r'AATT_y
Cuts at: 0 254 560 1320 1704
Size: 254 306 760 384
Fragments arranged by size:
760 384 306 254
AvaI C'yCGr_G
Cuts at: 0 1244 1704
Size: 1244 460
AvrII C'CTAG_G
Cuts at: 0 828 1704
Size: 828 876
BanI G'GyrC_C
Cuts at: 0 1252 1704
Size: 1252 452
BanII G_rGCy'C
Cuts at: 0 197 825 1704
Size: 197 628 879
Fragments arranged by size:
879 628 197
BbvCI CC'TCA_GC
Cuts at: 0 461 1704
Size: 461 1243
BlpI GC'TnA_GC
Cuts at: 0 713 1704
Size: 713 991
BmrI ACTGGGnnnn_n'
Cuts at: 0 615 1704
Size: 615 1089
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1136 1172 1704
Size: 1136 36 532
Fragments arranged by size:
1136 532 36
Bpu10I CC'TnA_GC
Cuts at: 0 461 1704
Size: 461 1243
BsaHI Gr'CG_yC
Cuts at: 0 1173 1704
Size: 1173 531
BsaWI w'CCGG_w
Cuts at: 0 986 1704
Size: 986 718
BseYI C'CCAG_C
Cuts at: 0 1224 1637 1664 1704
Size: 1224 413 27 40
Fragments arranged by size:
1224 413 40 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1415 1704
Size: 1415 289
BsiHKAI G_wGCw'C
Cuts at: 0 197 1704
Size: 197 1507
BsmBI CGTCTCn'nnnn_
Cuts at: 0 948 1704
Size: 948 756
Bsp1286I G_dGCh'C
Cuts at: 0 197 825 1704
Size: 197 628 879
Fragments arranged by size:
879 628 197
BspHI T'CATG_A
Cuts at: 0 981 1704
Size: 981 723
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 659 1704
Size: 659 1045
BsrBI CCG'CTC
Cuts at: 0 630 1704
Size: 630 1074
BsrGI T'GTAC_A
Cuts at: 0 272 306 1704
Size: 272 34 1398
Fragments arranged by size:
1398 272 34
BstXI CCAn_nnnn'nTGG
Cuts at: 0 891 1128 1704
Size: 891 237 576
Fragments arranged by size:
891 576 237
BstYI r'GATC_y
Cuts at: 0 747 989 1704
Size: 747 242 715
Fragments arranged by size:
747 715 242
Bsu36I CC'TnA_GG
Cuts at: 0 76 1704
Size: 76 1628
BtgI C'CryG_G
Cuts at: 0 248 1655 1704
Size: 248 1407 49
Fragments arranged by size:
1407 248 49
EaeI y'GGCC_r
Cuts at: 0 1014 1268 1376 1704
Size: 1014 254 108 328
Fragments arranged by size:
1014 328 254 108
EarI CTCTTCn'nnn_
Cuts at: 0 76 500 572 1704
Size: 76 424 72 1132
Fragments arranged by size:
1132 424 76 72
EcoICRI GAG'CTC
Cuts at: 0 195 1704
Size: 195 1509
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 509 596 1136 1172 1704
Size: 322 187 87 540 36 532
Fragments arranged by size:
540 532 322 187 87 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1346 1704
Size: 1346 358
EcoO109I rG'GnC_Cy
Cuts at: 0 808 1704
Size: 808 896
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 334 1704
Size: 334 1370
HaeII r_GCGC'y
Cuts at: 0 1194 1671 1704
Size: 1194 477 33
Fragments arranged by size:
1194 477 33
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 24 56 807 839 1124 1156 1272 1304
Size: 24 32 751 32 285 32 116 32
Cuts at: 1304 1704
Size: 400
Fragments arranged by size:
751 400 285 116 32 32 32 32
24
HincII GTy'rAC
Cuts at: 0 222 444 1704
Size: 222 222 1260
Fragments arranged by size:
1260 222 222
MfeI C'AATT_G
Cuts at: 0 435 1571 1704
Size: 435 1136 133
Fragments arranged by size:
1136 435 133
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 368 1125 1704
Size: 368 757 579
Fragments arranged by size:
757 579 368
MscI TGG'CCA
Cuts at: 0 1270 1378 1704
Size: 1270 108 326
Fragments arranged by size:
1270 326 108
MslI CAynn'nnrTG
Cuts at: 0 437 889 1183 1424 1513 1704
Size: 437 452 294 241 89 191
Fragments arranged by size:
452 437 294 241 191 89
MspA1I CmG'CkG
Cuts at: 0 460 728 1637 1704
Size: 460 268 909 67
Fragments arranged by size:
909 460 268 67
NcoI C'CATG_G
Cuts at: 0 248 1655 1704
Size: 248 1407 49
Fragments arranged by size:
1407 248 49
NspI r_CATG'y
Cuts at: 0 292 619 1224 1704
Size: 292 327 605 480
Fragments arranged by size:
605 480 327 292
PasI CC'CwG_GG
Cuts at: 0 1128 1704
Size: 1128 576
PciI A'CATG_T
Cuts at: 0 288 1704
Size: 288 1416
PfoI T'CCnGG_A
Cuts at: 0 1495 1704
Size: 1495 209
PpuMI rG'GwC_Cy
Cuts at: 0 808 1704
Size: 808 896
PshAI GACnn'nnGTC
Cuts at: 0 1504 1704
Size: 1504 200
PstI C_TGCA'G
Cuts at: 0 327 1704
Size: 327 1377
PvuII CAG'CTG
Cuts at: 0 460 1637 1704
Size: 460 1177 67
Fragments arranged by size:
1177 460 67
SacI G_AGCT'C
Cuts at: 0 197 1704
Size: 197 1507
SanDI GG'GwC_CC
Cuts at: 0 808 1704
Size: 808 896
SapI GCTCTTCn'nnn_
Cuts at: 0 76 572 1704
Size: 76 496 1132
Fragments arranged by size:
1132 496 76
SfcI C'TryA_G
Cuts at: 0 159 323 655 729 1704
Size: 159 164 332 74 975
Fragments arranged by size:
975 332 164 159 74
SmaI CCC'GGG
Cuts at: 0 1246 1704
Size: 1246 458
SpeI A'CTAG_T
Cuts at: 0 187 1704
Size: 187 1517
SphI G_CATG'C
Cuts at: 0 619 1224 1704
Size: 619 605 480
Fragments arranged by size:
619 605 480
StuI AGG'CCT
Cuts at: 0 783 1704
Size: 783 921
StyI C'CwwG_G
Cuts at: 0 228 248 828 1655 1704
Size: 228 20 580 827 49
Fragments arranged by size:
827 580 228 49 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 597 1704
Size: 597 1107
TatI w'GTAC_w
Cuts at: 0 272 306 1704
Size: 272 34 1398
Fragments arranged by size:
1398 272 34
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1250 1282 1447 1479 1704
Size: 1250 32 165 32 225
Fragments arranged by size:
1250 225 165 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1106 1704
Size: 1106 598
XbaI T'CTAG_A
Cuts at: 0 1044 1704
Size: 1044 660
XmaI C'CCGG_G
Cuts at: 0 1244 1704
Size: 1244 460
XmnI GAAnn'nnTTC
Cuts at: 0 127 317 1704
Size: 127 190 1387
Fragments arranged by size:
1387 190 127
Enzymes that do cut:
AcuI AfeI AflIII AleI ApoI AvaI AvrII BanI
BanII BbvCI BlpI BmrI BpmI Bpu10I BsaHI BsaWI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrGI BstXI BstYI Bsu36I BtgI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII MfeI
MmeI MscI MslI MspA1I NcoI NspI PasI PciI
PfoI PpuMI PshAI PstI PvuII SacI SanDI SapI
SfcI SmaI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
BaeI BamHI BbeI BbsI BcgI BciVI BclI BfrBI
BglI BglII Bme1580I BmgBI BmtI BplI BpuEI BsaI
BsaAI BsaBI BsaXI BseRI BsiEI BsiWI BsmI BspEI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII BstZ17I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI
EciI EcoRI EcoRV FseI FspI FspAI HindIII HpaI
KasI KpnI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PflMI PmeI PmlI PpiI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI
SnaBI SrfI SspI SwaI XcmI XhoI ZraI