Restriction map for
pF1KB5985
(Linear) (Six-base) MAPSORT of: KIBB5985.seq Check: 9630 from: 1 to: 1563
KIBB5985.orf
With 182 enzymes: SgfI *
June 26, 2008 10:45 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1258 1496 1563
Size: 1258 238 67
Fragments arranged by size:
1258 238 67
AfeI AGC'GCT
Cuts at: 0 1210 1563
Size: 1210 353
AflIII A'CryG_T
Cuts at: 0 1222 1563
Size: 1222 341
AgeI A'CCGG_T
Cuts at: 0 1382 1563
Size: 1382 181
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1489 1521 1563
Size: 1489 32 42
Fragments arranged by size:
1489 42 32
AlwNI CAG_nnn'CTG
Cuts at: 0 169 572 747 1563
Size: 169 403 175 816
Fragments arranged by size:
816 403 175 169
ApaI G_GGCC'C
Cuts at: 0 894 1077 1563
Size: 894 183 486
Fragments arranged by size:
894 486 183
AscI GG'CGCG_CC
Cuts at: 0 1369 1563
Size: 1369 194
AvaI C'yCGr_G
Cuts at: 0 18 34 894 1063 1313 1563
Size: 18 16 860 169 250 250
Fragments arranged by size:
860 250 250 169 18 16
BanI G'GyrC_C
Cuts at: 0 361 1563
Size: 361 1202
BanII G_rGCy'C
Cuts at: 0 43 256 894 1013 1077 1563
Size: 43 213 638 119 64 486
Fragments arranged by size:
638 486 213 119 64 43
BglI GCCn_nnn'nGGC
Cuts at: 0 889 1563
Size: 889 674
BlpI GC'TnA_GC
Cuts at: 0 398 1563
Size: 398 1165
Bme1580I G_kGCm'C
Cuts at: 0 894 1077 1314 1563
Size: 894 183 237 249
Fragments arranged by size:
894 249 237 183
BmrI ACTGGGnnnn_n'
Cuts at: 0 560 897 1563
Size: 560 337 666
Fragments arranged by size:
666 560 337
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 123 594 1381 1516 1563
Size: 123 471 787 135 47
Fragments arranged by size:
787 471 135 123 47
Bpu10I CC'TnA_GC
Cuts at: 0 1044 1081 1563
Size: 1044 37 482
Fragments arranged by size:
1044 482 37
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 131 970 1129 1563
Size: 131 839 159 434
Fragments arranged by size:
839 434 159 131
BsaI GGTCTCn'nnnn_
Cuts at: 0 166 316 1563
Size: 166 150 1247
Fragments arranged by size:
1247 166 150
BsaAI yAC'GTr
Cuts at: 0 1179 1223 1563
Size: 1179 44 340
Fragments arranged by size:
1179 340 44
BsaBI GATnn'nnATC
Cuts at: 0 661 1563
Size: 661 902
BsaHI Gr'CG_yC
Cuts at: 0 876 1280 1325 1563
Size: 876 404 45 238
Fragments arranged by size:
876 404 238 45
BsaWI w'CCGG_w
Cuts at: 0 128 773 1382 1563
Size: 128 645 609 181
Fragments arranged by size:
645 609 181 128
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1489 1519 1563
Size: 1489 30 44
Fragments arranged by size:
1489 44 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 703 988 1395 1563
Size: 703 285 407 168
Fragments arranged by size:
703 407 285 168
BseYI C'CCAG_C
Cuts at: 0 198 314 341 437 475 726 1014 1530
Size: 198 116 27 96 38 251 288 516
Cuts at: 1530 1563
Size: 33
Fragments arranged by size:
516 288 251 198 116 96 38 33
27
BsiEI CG_ry'CG
Cuts at: 0 930 1166 1563
Size: 930 236 397
Fragments arranged by size:
930 397 236
BsiHKAI G_wGCw'C
Cuts at: 0 139 1013 1563
Size: 139 874 550
Fragments arranged by size:
874 550 139
BsmBI CGTCTCn'nnnn_
Cuts at: 0 857 1563
Size: 857 706
Bsp1286I G_dGCh'C
Cuts at: 0 43 139 256 894 1013 1077 1314 1563
Size: 43 96 117 638 119 64 237 249
Fragments arranged by size:
638 249 237 119 117 96 64 43
BsrBI CCG'CTC
Cuts at: 0 1043 1563
Size: 1043 520
BsrFI r'CCGG_y
Cuts at: 0 780 1000 1382 1563
Size: 780 220 382 181
Fragments arranged by size:
780 382 220 181
BssHII G'CGCG_C
Cuts at: 0 1369 1563
Size: 1369 194
BssSI C'ACGA_G
Cuts at: 0 1156 1563
Size: 1156 407
BstYI r'GATC_y
Cuts at: 0 165 578 1499 1563
Size: 165 413 921 64
Fragments arranged by size:
921 413 165 64
Bsu36I CC'TnA_GG
Cuts at: 0 971 1563
Size: 971 592
BtgI C'CryG_G
Cuts at: 0 1319 1563
Size: 1319 244
BtsI GCAGTG_nn'
Cuts at: 0 194 1115 1563
Size: 194 921 448
Fragments arranged by size:
921 448 194
DraIII CAC_nnn'GTG
Cuts at: 0 1158 1563
Size: 1158 405
EaeI y'GGCC_r
Cuts at: 0 752 881 991 1163 1563
Size: 752 129 110 172 400
Fragments arranged by size:
752 400 172 129 110
EagI C'GGCC_G
Cuts at: 0 1163 1563
Size: 1163 400
EarI CTCTTCn'nnn_
Cuts at: 0 314 382 1351 1563
Size: 314 68 969 212
Fragments arranged by size:
969 314 212 68
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 344 915 1563
Size: 344 571 648
Fragments arranged by size:
648 571 344
EcoICRI GAG'CTC
Cuts at: 0 1011 1563
Size: 1011 552
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 123 594 1258 1381 1496 1516 1563
Size: 123 471 664 123 115 20 47
Fragments arranged by size:
664 471 123 123 115 47 20
EcoNI CCTnn'n_nnAGG
Cuts at: 0 115 1563
Size: 115 1448
EcoO109I rG'GnC_Cy
Cuts at: 0 15 106 224 732 891 1048 1073 1074
Size: 15 91 118 508 159 157 25 1
Cuts at: 1074 1461 1563
Size: 387 102
Fragments arranged by size:
508 387 159 157 118 102 91 25
15 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 930 1155 1563
Size: 930 225 408
Fragments arranged by size:
930 408 225
HaeII r_GCGC'y
Cuts at: 0 58 1088 1212 1563
Size: 58 1030 124 351
Fragments arranged by size:
1030 351 124 58
HincII GTy'rAC
Cuts at: 0 207 1563
Size: 207 1356
HpaI GTT'AAC
Cuts at: 0 207 1563
Size: 207 1356
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 1563
Size: 227 1336
MscI TGG'CCA
Cuts at: 0 754 993 1563
Size: 754 239 570
Fragments arranged by size:
754 570 239
MslI CAynn'nnrTG
Cuts at: 0 856 1432 1563
Size: 856 576 131
Fragments arranged by size:
856 576 131
MspA1I CmG'CkG
Cuts at: 0 198 475 1321 1337 1563
Size: 198 277 846 16 226
Fragments arranged by size:
846 277 226 198 16
NaeI GCC'GGC
Cuts at: 0 1002 1563
Size: 1002 561
NgoMIV G'CCGG_C
Cuts at: 0 1000 1563
Size: 1000 563
NspI r_CATG'y
Cuts at: 0 795 1563
Size: 795 768
PasI CC'CwG_GG
Cuts at: 0 46 489 1120 1563
Size: 46 443 631 443
Fragments arranged by size:
631 443 443 46
PflMI CCAn_nnn'nTGG
Cuts at: 0 572 747 1563
Size: 572 175 816
Fragments arranged by size:
816 572 175
PfoI T'CCnGG_A
Cuts at: 0 1493 1563
Size: 1493 70
PmlI CAC'GTG
Cuts at: 0 1179 1223 1563
Size: 1179 44 340
Fragments arranged by size:
1179 340 44
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1489 1521 1563
Size: 1489 32 42
Fragments arranged by size:
1489 42 32
PpuMI rG'GwC_Cy
Cuts at: 0 106 224 732 1563
Size: 106 118 508 831
Fragments arranged by size:
831 508 118 106
PspOMI G'GGCC_C
Cuts at: 0 890 1073 1563
Size: 890 183 490
Fragments arranged by size:
890 490 183
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1534 1563
Size: 1534 29
PstI C_TGCA'G
Cuts at: 0 809 1563
Size: 809 754
PvuI CG_AT'CG
Cuts at: 0 930 1563
Size: 930 633
PvuII CAG'CTG
Cuts at: 0 198 475 1563
Size: 198 277 1088
Fragments arranged by size:
1088 277 198
RsrII CG'GwC_CG
Cuts at: 0 126 1563
Size: 126 1437
SacI G_AGCT'C
Cuts at: 0 1013 1563
Size: 1013 550
SacII CC_GC'GG
Cuts at: 0 1322 1563
Size: 1322 241
SanDI GG'GwC_CC
Cuts at: 0 106 224 732 1563
Size: 106 118 508 831
Fragments arranged by size:
831 508 118 106
SfcI C'TryA_G
Cuts at: 0 805 1443 1563
Size: 805 638 120
Fragments arranged by size:
805 638 120
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 889 1563
Size: 889 674
SmaI CCC'GGG
Cuts at: 0 20 896 1563
Size: 20 876 667
Fragments arranged by size:
876 667 20
SmlI C'TyrA_G
Cuts at: 0 34 146 985 1144 1563
Size: 34 112 839 159 419
Fragments arranged by size:
839 419 159 112 34
StyI C'CwwG_G
Cuts at: 0 701 948 1563
Size: 701 247 615
Fragments arranged by size:
701 615 247
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 314 1506 1563
Size: 314 1192 57
Fragments arranged by size:
1192 314 57
TatI w'GTAC_w
Cuts at: 0 1541 1563
Size: 1541 22
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 118 150 1563
Size: 118 32 1413
Fragments arranged by size:
1413 118 32
Tth111I GACn'n_nGTC
Cuts at: 0 1429 1563
Size: 1429 134
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 147 1095 1563
Size: 147 948 468
Fragments arranged by size:
948 468 147
XhoI C'TCGA_G
Cuts at: 0 34 1563
Size: 34 1529
XmaI C'CCGG_G
Cuts at: 0 18 894 1563
Size: 18 876 669
Fragments arranged by size:
876 669 18
XmnI GAAnn'nnTTC
Cuts at: 0 913 1453 1563
Size: 913 540 110
Fragments arranged by size:
913 540 110
Enzymes that do cut:
AcuI AfeI AflIII AgeI AloI AlwNI ApaI AscI
AvaI BanI BanII BglI BlpI Bme1580I BmrI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I BsrBI BsrFI
BssHII BssSI BstYI Bsu36I BtgI BtsI DraIII EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
HaeII HincII HpaI MmeI MscI MslI MspA1I NaeI
NgoMIV NspI PasI PflMI PfoI PmlI PpiI PpuMI
PspOMI PsrI PstI PvuI PvuII RsrII SacI SacII
SanDI SfcI SfiI SmaI SmlI StyI TaqII TatI
TstI Tth111I XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AhdI AleI
AlfI ApaLI ApoI AseI AsiSI AvrII BaeI BamHI
BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglII
BmgBI BmtI BplI BsgI BsiWI BsmI BspEI BspHI
BspMI BsrDI BsrGI BstAPI BstBI BstEII BstXI BstZ17I
BtgZI ClaI CspCI DraI DrdI EcoRI EcoRV FseI
FspI FspAI Hin4I HindIII KasI KpnI MfeI MluI
NarI NcoI NdeI NheI NotI NruI NsiI PacI
PciI PmeI PshAI PsiI PspXI SalI SapI SbfI
ScaI SexAI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI XbaI ZraI