Restriction map for
pF1KB5986
(Linear) (Six-base) MAPSORT of: KIBB5986.seq Check: 5087 from: 1 to: 1707
KIBB5986.orf
With 182 enzymes: SgfI *
February 8, 2013 18:16 ..
AccI GT'mk_AC
Cuts at: 0 713 1195 1707
Size: 713 482 512
Fragments arranged by size:
713 512 482
Acc65I G'GTAC_C
Cuts at: 0 1604 1707
Size: 1604 103
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1121 1365 1424 1707
Size: 1121 244 59 283
Fragments arranged by size:
1121 283 244 59
AleI CACnn'nnGTG
Cuts at: 0 521 1707
Size: 521 1186
AlwNI CAG_nnn'CTG
Cuts at: 0 1653 1707
Size: 1653 54
ApoI r'AATT_y
Cuts at: 0 297 334 640 1510 1707
Size: 297 37 306 870 197
Fragments arranged by size:
870 306 297 197 37
AseI AT'TA_AT
Cuts at: 0 1319 1707
Size: 1319 388
AvaI C'yCGr_G
Cuts at: 0 629 1702 1707
Size: 629 1073 5
Fragments arranged by size:
1073 629 5
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 179 212 1707
Size: 179 33 1495
Fragments arranged by size:
1495 179 33
BamHI G'GATC_C
Cuts at: 0 721 1707
Size: 721 986
BanI G'GyrC_C
Cuts at: 0 1604 1707
Size: 1604 103
BanII G_rGCy'C
Cuts at: 0 691 1707
Size: 691 1016
BbsI GAAGACnn'nnnn_
Cuts at: 0 833 1307 1350 1707
Size: 833 474 43 357
Fragments arranged by size:
833 474 357 43
BciVI GTATCCnnnnn_n'
Cuts at: 0 1181 1707
Size: 1181 526
BfrBI ATG'CAT
Cuts at: 0 592 1707
Size: 592 1115
Bme1580I G_kGCm'C
Cuts at: 0 427 1707
Size: 427 1280
BmrI ACTGGGnnnn_n'
Cuts at: 0 808 1707
Size: 808 899
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 198 1489 1707
Size: 73 125 1291 218
Fragments arranged by size:
1291 218 125 73
Bpu10I CC'TnA_GC
Cuts at: 0 257 1707
Size: 257 1450
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1483 1707
Size: 1483 224
BsaAI yAC'GTr
Cuts at: 0 1695 1707
Size: 1695 12
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 404 434 1707
Size: 404 30 1273
Fragments arranged by size:
1273 404 30
BseYI C'CCAG_C
Cuts at: 0 690 1389 1707
Size: 690 699 318
Fragments arranged by size:
699 690 318
BsmBI CGTCTCn'nnnn_
Cuts at: 0 401 1707
Size: 401 1306
Bsp1286I G_dGCh'C
Cuts at: 0 427 691 1707
Size: 427 264 1016
Fragments arranged by size:
1016 427 264
BsrDI GCAATG_nn'
Cuts at: 0 767 1707
Size: 767 940
BsrFI r'CCGG_y
Cuts at: 0 751 1707
Size: 751 956
BstEII G'GTnAC_C
Cuts at: 0 1628 1707
Size: 1628 79
BstYI r'GATC_y
Cuts at: 0 721 1707
Size: 721 986
Bsu36I CC'TnA_GG
Cuts at: 0 1235 1707
Size: 1235 472
ClaI AT'CG_AT
Cuts at: 0 1504 1707
Size: 1504 203
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 320 355 1707
Size: 320 35 1352
Fragments arranged by size:
1352 320 35
DraI TTT'AAA
Cuts at: 0 444 1224 1707
Size: 444 780 483
Fragments arranged by size:
780 483 444
EaeI y'GGCC_r
Cuts at: 0 753 1707
Size: 753 954
EarI CTCTTCn'nnn_
Cuts at: 0 1400 1707
Size: 1400 307
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 198 1121 1365 1424 1489 1707
Size: 73 125 923 244 59 65 218
Fragments arranged by size:
923 244 218 125 73 65 59
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 256 1707
Size: 256 1451
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 33 65 706 738 1337 1369 1707
Size: 33 32 641 32 599 32 338
Fragments arranged by size:
641 599 338 33 32 32 32
HincII GTy'rAC
Cuts at: 0 1034 1707
Size: 1034 673
HindIII A'AGCT_T
Cuts at: 0 1133 1707
Size: 1133 574
KpnI G_GTAC'C
Cuts at: 0 1608 1707
Size: 1608 99
MfeI C'AATT_G
Cuts at: 0 733 1707
Size: 733 974
MslI CAynn'nnrTG
Cuts at: 0 521 547 880 1045 1707
Size: 521 26 333 165 662
Fragments arranged by size:
662 521 333 165 26
MspA1I CmG'CkG
Cuts at: 0 1385 1541 1707
Size: 1385 156 166
Fragments arranged by size:
1385 166 156
NsiI A_TGCA'T
Cuts at: 0 594 1707
Size: 594 1113
NspI r_CATG'y
Cuts at: 0 1614 1707
Size: 1614 93
PfoI T'CCnGG_A
Cuts at: 0 214 1707
Size: 214 1493
PmlI CAC'GTG
Cuts at: 0 1695 1707
Size: 1695 12
PshAI GACnn'nnGTC
Cuts at: 0 1569 1707
Size: 1569 138
PsiI TTA'TAA
Cuts at: 0 365 917 1707
Size: 365 552 790
Fragments arranged by size:
790 552 365
PvuII CAG'CTG
Cuts at: 0 1385 1707
Size: 1385 322
SapI GCTCTTCn'nnn_
Cuts at: 0 1400 1707
Size: 1400 307
SfcI C'TryA_G
Cuts at: 0 200 1707
Size: 200 1507
SmaI CCC'GGG
Cuts at: 0 631 1707
Size: 631 1076
SmlI C'TyrA_G
Cuts at: 0 1462 1707
Size: 1462 245
StuI AGG'CCT
Cuts at: 0 675 1707
Size: 675 1032
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1381 1707
Size: 1381 326
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 404 436 1237 1269 1707
Size: 404 32 801 32 438
Fragments arranged by size:
801 438 404 32 32
XbaI T'CTAG_A
Cuts at: 0 651 1707
Size: 651 1056
XmaI C'CCGG_G
Cuts at: 0 629 1707
Size: 629 1078
Enzymes that do cut:
AccI Acc65I AcuI AleI AlwNI ApoI AseI AvaI
BaeI BamHI BanI BanII BbsI BciVI BfrBI Bme1580I
BmrI BpmI Bpu10I BpuEI BsaAI BsaXI BseYI BsmBI
Bsp1286I BsrDI BsrFI BstEII BstYI Bsu36I ClaI CspCI
DraI EaeI EarI Eco57MI FalI Hin4I HincII HindIII
KpnI MfeI MslI MspA1I NsiI NspI PfoI PmlI
PshAI PsiI PvuII SapI SfcI SmaI SmlI StuI
TaqII TstI XbaI XmaI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AsiSI AvrII BbeI
BbvCI BcgI BclI BglI BglII BlpI BmgBI BmtI
BplI BsaI BsaBI BsaHI BsaWI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmI BspEI BspHI BspMI BsrBI BsrGI
BssHII BssSI BstAPI BstBI BstXI BstZ17I BtgI BtgZI
BtsI DraIII DrdI EagI EciI EcoICRI EcoNI EcoO109I
EcoRI EcoRV FseI FspI FspAI HaeII HpaI KasI
MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI PacI PasI PciI PflMI PmeI
PpiI PpuMI PspOMI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StyI SwaI TatI Tth111I XcmI XhoI XmnI ZraI