Restriction map for
pF1KB5987
(Linear) (Six-base) MAPSORT of: KIBB5987.seq Check: 3867 from: 1 to: 1710
KIBB5987.orf
With 182 enzymes: SgfI *
October 20, 2011 13:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1617 1710
Size: 1617 93
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1561 1710
Size: 1561 149
AflIII A'CryG_T
Cuts at: 0 631 1710
Size: 631 1079
AleI CACnn'nnGTG
Cuts at: 0 9 1710
Size: 9 1701
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 71 1710
Size: 71 1639
AlwNI CAG_nnn'CTG
Cuts at: 0 1613 1710
Size: 1613 97
ApoI r'AATT_y
Cuts at: 0 163 555 1255 1294 1710
Size: 163 392 700 39 416
Fragments arranged by size:
700 416 392 163 39
AvaI C'yCGr_G
Cuts at: 0 262 1475 1710
Size: 262 1213 235
Fragments arranged by size:
1213 262 235
BanII G_rGCy'C
Cuts at: 0 1173 1448 1710
Size: 1173 275 262
Fragments arranged by size:
1173 275 262
BciVI GTATCCnnnnn_n'
Cuts at: 0 1236 1294 1607 1710
Size: 1236 58 313 103
Fragments arranged by size:
1236 313 103 58
BclI T'GATC_A
Cuts at: 0 747 1710
Size: 747 963
Bme1580I G_kGCm'C
Cuts at: 0 142 725 1710
Size: 142 583 985
Fragments arranged by size:
985 583 142
BmrI ACTGGGnnnn_n'
Cuts at: 0 87 427 1710
Size: 87 340 1283
Fragments arranged by size:
1283 340 87
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1405 1462 1710
Size: 1405 57 248
Fragments arranged by size:
1405 248 57
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 609 961 1432 1710
Size: 256 353 352 471 278
Fragments arranged by size:
471 353 352 278 256
BsaAI yAC'GTr
Cuts at: 0 1416 1710
Size: 1416 294
BsaHI Gr'CG_yC
Cuts at: 0 1386 1710
Size: 1386 324
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1085 1115 1256 1286 1710
Size: 1085 30 141 30 424
Fragments arranged by size:
1085 424 141 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 253 304 793 1710
Size: 253 51 489 917
Fragments arranged by size:
917 489 253 51
BsiHKAI G_wGCw'C
Cuts at: 0 1421 1448 1562 1710
Size: 1421 27 114 148
Fragments arranged by size:
1421 148 114 27
BsiWI C'GTAC_G
Cuts at: 0 661 1710
Size: 661 1049
BsmI GAATG_Cn'
Cuts at: 0 461 1245 1710
Size: 461 784 465
Fragments arranged by size:
784 465 461
Bsp1286I G_dGCh'C
Cuts at: 0 142 725 1173 1421 1448 1562 1710
Size: 142 583 448 248 27 114 148
Fragments arranged by size:
583 448 248 148 142 114 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 346 1598 1617 1710
Size: 346 1252 19 93
Fragments arranged by size:
1252 346 93 19
BsrDI GCAATG_nn'
Cuts at: 0 599 837 1710
Size: 599 238 873
Fragments arranged by size:
873 599 238
BstAPI GCAn_nnn'nTGC
Cuts at: 0 137 1050 1240 1710
Size: 137 913 190 470
Fragments arranged by size:
913 470 190 137
BstXI CCAn_nnnn'nTGG
Cuts at: 0 190 1710
Size: 190 1520
BstYI r'GATC_y
Cuts at: 0 972 1710
Size: 972 738
BtgI C'CryG_G
Cuts at: 0 1275 1710
Size: 1275 435
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 86 1710
Size: 86 1624
BtsI GCAGTG_nn'
Cuts at: 0 725 1674 1710
Size: 725 949 36
Fragments arranged by size:
949 725 36
EaeI y'GGCC_r
Cuts at: 0 1631 1710
Size: 1631 79
EarI CTCTTCn'nnn_
Cuts at: 0 1537 1710
Size: 1537 173
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 244 1710
Size: 244 1466
EcoICRI GAG'CTC
Cuts at: 0 1446 1710
Size: 1446 264
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1405 1462 1561 1710
Size: 1405 57 99 149
Fragments arranged by size:
1405 149 99 57
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1233 1710
Size: 1233 477
EcoO109I rG'GnC_Cy
Cuts at: 0 231 1680 1710
Size: 231 1449 30
Fragments arranged by size:
1449 231 30
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 462 494 810 842 1085 1117 1710
Size: 462 32 316 32 243 32 593
Fragments arranged by size:
593 462 316 243 32 32 32
MscI TGG'CCA
Cuts at: 0 1633 1710
Size: 1633 77
MslI CAynn'nnrTG
Cuts at: 0 9 1639 1710
Size: 9 1630 71
Fragments arranged by size:
1630 71 9
MspA1I CmG'CkG
Cuts at: 0 190 407 1623 1710
Size: 190 217 1216 87
Fragments arranged by size:
1216 217 190 87
NspI r_CATG'y
Cuts at: 0 345 635 1710
Size: 345 290 1075
Fragments arranged by size:
1075 345 290
PciI A'CATG_T
Cuts at: 0 631 1710
Size: 631 1079
PflMI CCAn_nnn'nTGG
Cuts at: 0 655 1710
Size: 655 1055
PfoI T'CCnGG_A
Cuts at: 0 1439 1710
Size: 1439 271
PpuMI rG'GwC_Cy
Cuts at: 0 231 1710
Size: 231 1479
PsiI TTA'TAA
Cuts at: 0 545 1710
Size: 545 1165
PstI C_TGCA'G
Cuts at: 0 1628 1710
Size: 1628 82
PvuII CAG'CTG
Cuts at: 0 190 407 1623 1710
Size: 190 217 1216 87
Fragments arranged by size:
1216 217 190 87
SacI G_AGCT'C
Cuts at: 0 1448 1710
Size: 1448 262
SfcI C'TryA_G
Cuts at: 0 171 1624 1710
Size: 171 1453 86
Fragments arranged by size:
1453 171 86
SmlI C'TyrA_G
Cuts at: 0 235 588 976 1447 1710
Size: 235 353 388 471 263
Fragments arranged by size:
471 388 353 263 235
StyI C'CwwG_G
Cuts at: 0 383 1034 1710
Size: 383 651 676
Fragments arranged by size:
676 651 383
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1337 1710
Size: 1337 373
TatI w'GTAC_w
Cuts at: 0 1500 1505 1710
Size: 1500 5 205
Fragments arranged by size:
1500 205 5
XmnI GAAnn'nnTTC
Cuts at: 0 392 1710
Size: 392 1318
Enzymes that do cut:
AarI AcuI AflIII AleI AlfI AlwNI ApoI AvaI
BanII BciVI BclI Bme1580I BmrI BpmI BpuEI BsaAI
BsaHI BsaXI BseRI BsiHKAI BsiWI BsmI Bsp1286I BspMI
BsrDI BstAPI BstXI BstYI BtgI BtgZI BtsI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I Hin4I MscI
MslI MspA1I NspI PciI PflMI PfoI PpuMI PsiI
PstI PvuII SacI SfcI SmlI StyI TaqII TatI
XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BbsI BbvCI BcgI BfrBI BglI
BglII BlpI BmgBI BmtI BplI Bpu10I BsaI BsaBI
BsaWI BseYI BsgI BsiEI BsmBI BspEI BspHI BsrBI
BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I
ClaI CspCI DraI DraIII DrdI EagI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TstI Tth111I XbaI XcmI
XhoI XmaI ZraI