Restriction map for
pF1KB5992
(Linear) (Six-base) MAPSORT of: KIBB5992.seq Check: 6043 from: 1 to: 1827
KIBB5992.orf
With 182 enzymes: SgfI *
July 10, 2012 13:27 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 233 989 1013 1518 1799 1827
Size: 233 756 24 505 281 28
Fragments arranged by size:
756 505 281 233 28 24
AfeI AGC'GCT
Cuts at: 0 1631 1827
Size: 1631 196
AhdI GACnn_n'nnGTC
Cuts at: 0 882 1827
Size: 882 945
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 615 1827
Size: 615 1212
AlwNI CAG_nnn'CTG
Cuts at: 0 430 1827
Size: 430 1397
ApoI r'AATT_y
Cuts at: 0 39 308 481 684 1827
Size: 39 269 173 203 1143
Fragments arranged by size:
1143 269 203 173 39
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1437 1470 1827
Size: 1437 33 357
Fragments arranged by size:
1437 357 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 239 366 464 1086 1827
Size: 239 127 98 622 741
Fragments arranged by size:
741 622 239 127 98
BbvCI CC'TCA_GC
Cuts at: 0 1088 1827
Size: 1088 739
BciVI GTATCCnnnnn_n'
Cuts at: 0 1120 1827
Size: 1120 707
BfrBI ATG'CAT
Cuts at: 0 208 667 1827
Size: 208 459 1160
Fragments arranged by size:
1160 459 208
BmrI ACTGGGnnnn_n'
Cuts at: 0 150 1827
Size: 150 1677
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 254 380 1457 1827
Size: 254 126 1077 370
Fragments arranged by size:
1077 370 254 126
Bpu10I CC'TnA_GC
Cuts at: 0 1088 1619 1827
Size: 1088 531 208
Fragments arranged by size:
1088 531 208
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1723 1827
Size: 1723 104
BsaBI GATnn'nnATC
Cuts at: 0 1345 1827
Size: 1345 482
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 827 1827
Size: 827 1000
BseYI C'CCAG_C
Cuts at: 0 1319 1827
Size: 1319 508
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 692 1485 1827
Size: 692 793 342
Fragments arranged by size:
793 692 342
BsiHKAI G_wGCw'C
Cuts at: 0 803 1827
Size: 803 1024
BsmI GAATG_Cn'
Cuts at: 0 604 907 1180 1573 1827
Size: 604 303 273 393 254
Fragments arranged by size:
604 393 303 273 254
Bsp1286I G_dGCh'C
Cuts at: 0 803 1827
Size: 803 1024
BspHI T'CATG_A
Cuts at: 0 473 1065 1827
Size: 473 592 762
Fragments arranged by size:
762 592 473
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 282 1827
Size: 282 1545
BsrBI CCG'CTC
Cuts at: 0 1426 1827
Size: 1426 401
BsrDI GCAATG_nn'
Cuts at: 0 1712 1827
Size: 1712 115
BsrGI T'GTAC_A
Cuts at: 0 792 1827
Size: 792 1035
BssSI C'ACGA_G
Cuts at: 0 1808 1827
Size: 1808 19
BstAPI GCAn_nnn'nTGC
Cuts at: 0 214 1827
Size: 214 1613
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1359 1827
Size: 1359 468
BstYI r'GATC_y
Cuts at: 0 1605 1827
Size: 1605 222
BtsI GCAGTG_nn'
Cuts at: 0 58 1827
Size: 58 1769
ClaI AT'CG_AT
Cuts at: 0 403 1827
Size: 403 1424
EaeI y'GGCC_r
Cuts at: 0 1343 1491 1827
Size: 1343 148 336
Fragments arranged by size:
1343 336 148
EarI CTCTTCn'nnn_
Cuts at: 0 347 1258 1775 1827
Size: 347 911 517 52
Fragments arranged by size:
911 517 347 52
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 233 254 380 989 1013 1457 1518 1799
Size: 233 21 126 609 24 444 61 281
Cuts at: 1799 1827
Size: 28
Fragments arranged by size:
609 444 281 233 126 61 28 24
21
EcoO109I rG'GnC_Cy
Cuts at: 0 692 1827
Size: 692 1135
HaeII r_GCGC'y
Cuts at: 0 1633 1827
Size: 1633 194
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 229 261 868 900 1827
Size: 229 32 607 32 927
Fragments arranged by size:
927 607 229 32 32
MfeI C'AATT_G
Cuts at: 0 218 1827
Size: 218 1609
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1313 1715 1827
Size: 1313 402 112
Fragments arranged by size:
1313 402 112
MscI TGG'CCA
Cuts at: 0 1345 1493 1827
Size: 1345 148 334
Fragments arranged by size:
1345 334 148
MslI CAynn'nnrTG
Cuts at: 0 68 668 794 1827
Size: 68 600 126 1033
Fragments arranged by size:
1033 600 126 68
MspA1I CmG'CkG
Cuts at: 0 1323 1827
Size: 1323 504
NdeI CA'TA_TG
Cuts at: 0 450 862 1512 1560 1827
Size: 450 412 650 48 267
Fragments arranged by size:
650 450 412 267 48
NsiI A_TGCA'T
Cuts at: 0 210 669 1827
Size: 210 459 1158
Fragments arranged by size:
1158 459 210
NspI r_CATG'y
Cuts at: 0 667 671 1827
Size: 667 4 1156
Fragments arranged by size:
1156 667 4
PflMI CCAn_nnn'nTGG
Cuts at: 0 1230 1827
Size: 1230 597
PfoI T'CCnGG_A
Cuts at: 0 776 1214 1827
Size: 776 438 613
Fragments arranged by size:
776 613 438
PpuMI rG'GwC_Cy
Cuts at: 0 692 1827
Size: 692 1135
PstI C_TGCA'G
Cuts at: 0 117 343 836 1827
Size: 117 226 493 991
Fragments arranged by size:
991 493 226 117
PvuII CAG'CTG
Cuts at: 0 1323 1827
Size: 1323 504
SanDI GG'GwC_CC
Cuts at: 0 692 1827
Size: 692 1135
SfcI C'TryA_G
Cuts at: 0 113 339 832 1827
Size: 113 226 493 995
Fragments arranged by size:
995 493 226 113
SmlI C'TyrA_G
Cuts at: 0 1702 1827
Size: 1702 125
SpeI A'CTAG_T
Cuts at: 0 771 1827
Size: 771 1056
SspI AAT'ATT
Cuts at: 0 1072 1273 1827
Size: 1072 201 554
Fragments arranged by size:
1072 554 201
StuI AGG'CCT
Cuts at: 0 1730 1827
Size: 1730 97
StyI C'CwwG_G
Cuts at: 0 1749 1827
Size: 1749 78
TatI w'GTAC_w
Cuts at: 0 109 792 1827
Size: 109 683 1035
Fragments arranged by size:
1035 683 109
Tth111I GACn'n_nGTC
Cuts at: 0 1329 1827
Size: 1329 498
XmnI GAAnn'nnTTC
Cuts at: 0 84 481 1150 1827
Size: 84 397 669 677
Fragments arranged by size:
677 669 397 84
Enzymes that do cut:
AcuI AfeI AhdI AlfI AlwNI ApoI BaeI BbsI
BbvCI BciVI BfrBI BmrI BpmI Bpu10I BpuEI BsaBI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrGI BssSI BstAPI BstXI BstYI BtsI
ClaI EaeI EarI Eco57MI EcoO109I HaeII Hin4I MfeI
MmeI MscI MslI MspA1I NdeI NsiI NspI PflMI
PfoI PpuMI PstI PvuII SanDI SfcI SmlI SpeI
SspI StuI StyI TatI Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AleI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BcgI BclI BglI
BglII BlpI Bme1580I BmgBI BmtI BplI BsaI BsaAI
BsaHI BsaWI BsaXI BsiEI BsiWI BsmBI BspEI BsrFI
BssHII BstBI BstEII BstZ17I Bsu36I BtgI BtgZI CspCI
DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MluI NaeI NarI NcoI NgoMIV NheI
NotI NruI PacI PasI PciI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SphI SrfI SwaI TaqII
TstI XbaI XcmI XhoI XmaI ZraI