Restriction map for
pF1KB6003
(Linear) (Six-base) MAPSORT of: KIBB6003.seq Check: 8361 from: 1 to: 3594
KIBB6003.orf
With 182 enzymes: SgfI *
July 15, 2010 18:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 147 3594
Size: 147 3447
AatII G_ACGT'C
Cuts at: 0 3211 3594
Size: 3211 383
AccI GT'mk_AC
Cuts at: 0 507 3452 3594
Size: 507 2945 142
Fragments arranged by size:
2945 507 142
AclI AA'CG_TT
Cuts at: 0 809 3594
Size: 809 2785
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 443 1653 1909 2125 2206 2351 2620 3496
Size: 443 1210 256 216 81 145 269 876
Cuts at: 3496 3592 3594
Size: 96 2
Fragments arranged by size:
1210 876 443 269 256 216 145 96
81 2
AflII C'TTAA_G
Cuts at: 0 1170 3594
Size: 1170 2424
AflIII A'CryG_T
Cuts at: 0 2700 3594
Size: 2700 894
AleI CACnn'nnGTG
Cuts at: 0 3486 3594
Size: 3486 108
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3294 3594
Size: 3294 300
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3193 3225 3594
Size: 3193 32 369
Fragments arranged by size:
3193 369 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2319 2859 3294 3594
Size: 2319 540 435 300
Fragments arranged by size:
2319 540 435 300
ApaLI G'TGCA_C
Cuts at: 0 2549 3594
Size: 2549 1045
ApoI r'AATT_y
Cuts at: 0 269 485 1760 1938 2385 2463 2512 3469
Size: 269 216 1275 178 447 78 49 957
Cuts at: 3469 3594
Size: 125
Fragments arranged by size:
1275 957 447 269 216 178 125 78
49
AseI AT'TA_AT
Cuts at: 0 205 1778 1832 3594
Size: 205 1573 54 1762
Fragments arranged by size:
1762 1573 205 54
AvaI C'yCGr_G
Cuts at: 0 3517 3594
Size: 3517 77
BamHI G'GATC_C
Cuts at: 0 1595 3594
Size: 1595 1999
BanI G'GyrC_C
Cuts at: 0 354 3172 3594
Size: 354 2818 422
Fragments arranged by size:
2818 422 354
BanII G_rGCy'C
Cuts at: 0 2342 3594
Size: 2342 1252
BbsI GAAGACnn'nnnn_
Cuts at: 0 335 584 1412 1915 3594
Size: 335 249 828 503 1679
Fragments arranged by size:
1679 828 503 335 249
BbvCI CC'TCA_GC
Cuts at: 0 67 3594
Size: 67 3527
BfrBI ATG'CAT
Cuts at: 0 1471 3042 3594
Size: 1471 1571 552
Fragments arranged by size:
1571 1471 552
BglI GCCn_nnn'nGGC
Cuts at: 0 3397 3594
Size: 3397 197
BglII A'GATC_T
Cuts at: 0 1057 3594
Size: 1057 2537
BlpI GC'TnA_GC
Cuts at: 0 1049 3594
Size: 1049 2545
Bme1580I G_kGCm'C
Cuts at: 0 359 2124 2553 3329 3594
Size: 359 1765 429 776 265
Fragments arranged by size:
1765 776 429 359 265
BmrI ACTGGGnnnn_n'
Cuts at: 0 132 3594
Size: 132 3462
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 440 726 1048 1051 1338 1905 3207 3264
Size: 440 286 322 3 287 567 1302 57
Cuts at: 3264 3594
Size: 330
Fragments arranged by size:
1302 567 440 330 322 287 286 57
3
Bpu10I CC'TnA_GC
Cuts at: 0 67 3594
Size: 67 3527
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 546 2146 3594
Size: 546 1600 1448
Fragments arranged by size:
1600 1448 546
BsaAI yAC'GTr
Cuts at: 0 3456 3594
Size: 3456 138
BsaHI Gr'CG_yC
Cuts at: 0 2571 3208 3377 3594
Size: 2571 637 169 217
Fragments arranged by size:
2571 637 217 169
BsaWI w'CCGG_w
Cuts at: 0 1575 3594
Size: 1575 2019
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 429 459 3091 3121 3594
Size: 429 30 2632 30 473
Fragments arranged by size:
2632 473 429 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 55 1174 2374 2449 3594
Size: 55 1119 1200 75 1145
Fragments arranged by size:
1200 1145 1119 75 55
BseYI C'CCAG_C
Cuts at: 0 2869 2897 3528 3594
Size: 2869 28 631 66
Fragments arranged by size:
2869 631 66 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 542 3070 3280 3594
Size: 542 2528 210 314
Fragments arranged by size:
2528 542 314 210
BsiHKAI G_wGCw'C
Cuts at: 0 756 825 947 2553 3594
Size: 756 69 122 1606 1041
Fragments arranged by size:
1606 1041 756 122 69
BsmI GAATG_Cn'
Cuts at: 0 2887 3594
Size: 2887 707
Bsp1286I G_dGCh'C
Cuts at: 0 359 756 825 947 2124 2342 2553 3329
Size: 359 397 69 122 1177 218 211 776
Cuts at: 3329 3594
Size: 265
Fragments arranged by size:
1177 776 397 359 265 218 211 122
69
BspEI T'CCGG_A
Cuts at: 0 1575 3594
Size: 1575 2019
BspHI T'CATG_A
Cuts at: 0 189 645 1185 2259 3594
Size: 189 456 540 1074 1335
Fragments arranged by size:
1335 1074 540 456 189
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 147 3594
Size: 147 3447
BsrDI GCAATG_nn'
Cuts at: 0 785 923 1263 2448 3008 3024 3453 3467
Size: 785 138 340 1185 560 16 429 14
Cuts at: 3467 3594
Size: 127
Fragments arranged by size:
1185 785 560 429 340 138 127 16
14
BsrFI r'CCGG_y
Cuts at: 0 26 3594
Size: 26 3568
BsrGI T'GTAC_A
Cuts at: 0 534 1516 2222 3594
Size: 534 982 706 1372
Fragments arranged by size:
1372 982 706 534
BssSI C'ACGA_G
Cuts at: 0 98 672 3033 3594
Size: 98 574 2361 561
Fragments arranged by size:
2361 574 561 98
BstEII G'GTnAC_C
Cuts at: 0 634 3594
Size: 634 2960
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3061 3192 3594
Size: 3061 131 402
Fragments arranged by size:
3061 402 131
BstYI r'GATC_y
Cuts at: 0 372 1057 1595 1722 3594
Size: 372 685 538 127 1872
Fragments arranged by size:
1872 685 538 372 127
BstZ17I GTA'TAC
Cuts at: 0 508 3594
Size: 508 3086
Bsu36I CC'TnA_GG
Cuts at: 0 360 3594
Size: 360 3234
BtgI C'CryG_G
Cuts at: 0 3395 3594
Size: 3395 199
BtsI GCAGTG_nn'
Cuts at: 0 1617 2253 3594
Size: 1617 636 1341
Fragments arranged by size:
1617 1341 636
DraIII CAC_nnn'GTG
Cuts at: 0 942 3594
Size: 942 2652
EaeI y'GGCC_r
Cuts at: 0 24 1982 2045 2624 3341 3594
Size: 24 1958 63 579 717 253
Fragments arranged by size:
1958 717 579 253 63 24
EarI CTCTTCn'nnn_
Cuts at: 0 275 771 1628 2330 3594
Size: 275 496 857 702 1264
Fragments arranged by size:
1264 857 702 496 275
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 66 3274 3594
Size: 66 3208 320
Fragments arranged by size:
3208 320 66
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 440 443 726 1048 1051 1338 1653 1905
Size: 440 3 283 322 3 287 315 252
Cuts at: 1905 1909 2125 2206 2351 2620 3207 3264 3496
Size: 4 216 81 145 269 587 57 232
Cuts at: 3496 3592 3594
Size: 96 2
Fragments arranged by size:
587 440 322 315 287 283 269 252
232 216 145 96 81 57 4 3
3 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2534 3286 3594
Size: 2534 752 308
Fragments arranged by size:
2534 752 308
EcoO109I rG'GnC_Cy
Cuts at: 0 104 366 2364 2608 3216 3594
Size: 104 262 1998 244 608 378
Fragments arranged by size:
1998 608 378 262 244 104
EcoRI G'AATT_C
Cuts at: 0 2385 2463 3594
Size: 2385 78 1131
Fragments arranged by size:
2385 1131 78
EcoRV GAT'ATC
Cuts at: 0 1305 1401 3594
Size: 1305 96 2193
Fragments arranged by size:
2193 1305 96
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 375 3594
Size: 375 3219
HaeII r_GCGC'y
Cuts at: 0 3588 3594
Size: 3588 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 427 459 534 566 1330 1362 1369 1401
Size: 427 32 75 32 764 32 7 32
Cuts at: 1401 1580 1612 3594
Size: 179 32 1982
Fragments arranged by size:
1982 764 427 179 75 32 32 32
32 32 7
HincII GTy'rAC
Cuts at: 0 228 798 1245 1977 3110 3594
Size: 228 570 447 732 1133 484
Fragments arranged by size:
1133 732 570 484 447 228
HindIII A'AGCT_T
Cuts at: 0 2039 3508 3594
Size: 2039 1469 86
Fragments arranged by size:
2039 1469 86
HpaI GTT'AAC
Cuts at: 0 228 3110 3594
Size: 228 2882 484
Fragments arranged by size:
2882 484 228
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 3095 3594
Size: 3095 499
MscI TGG'CCA
Cuts at: 0 1984 2047 2626 3343 3594
Size: 1984 63 579 717 251
Fragments arranged by size:
1984 717 579 251 63
MslI CAynn'nnrTG
Cuts at: 0 188 205 2015 3037 3059 3486 3594
Size: 188 17 1810 1022 22 427 108
Fragments arranged by size:
1810 1022 427 188 108 22 17
MspA1I CmG'CkG
Cuts at: 0 2198 2897 3594
Size: 2198 699 697
Fragments arranged by size:
2198 699 697
NcoI C'CATG_G
Cuts at: 0 3395 3594
Size: 3395 199
NsiI A_TGCA'T
Cuts at: 0 1473 3044 3594
Size: 1473 1571 550
Fragments arranged by size:
1571 1473 550
NspI r_CATG'y
Cuts at: 0 514 1009 3594
Size: 514 495 2585
Fragments arranged by size:
2585 514 495
PflMI CCAn_nnn'nTGG
Cuts at: 0 122 2859 3368 3594
Size: 122 2737 509 226
Fragments arranged by size:
2737 509 226 122
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 655 687 2430 2462 3594
Size: 655 32 1743 32 1132
Fragments arranged by size:
1743 1132 655 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 366 2364 2608 3216 3594
Size: 366 1998 244 608 378
Fragments arranged by size:
1998 608 378 366 244
PshAI GACnn'nnGTC
Cuts at: 0 416 632 3594
Size: 416 216 2962
Fragments arranged by size:
2962 416 216
PstI C_TGCA'G
Cuts at: 0 2653 3594
Size: 2653 941
SapI GCTCTTCn'nnn_
Cuts at: 0 2330 3594
Size: 2330 1264
SfcI C'TryA_G
Cuts at: 0 655 1625 2649 3594
Size: 655 970 1024 945
Fragments arranged by size:
1024 970 945 655
SmlI C'TyrA_G
Cuts at: 0 525 1170 2125 3594
Size: 525 645 955 1469
Fragments arranged by size:
1469 955 645 525
SphI G_CATG'C
Cuts at: 0 1009 3594
Size: 1009 2585
SspI AAT'ATT
Cuts at: 0 2299 3594
Size: 2299 1295
StuI AGG'CCT
Cuts at: 0 653 2131 2143 3594
Size: 653 1478 12 1451
Fragments arranged by size:
1478 1451 653 12
StyI C'CwwG_G
Cuts at: 0 350 2810 3258 3395 3594
Size: 350 2460 448 137 199
Fragments arranged by size:
2460 448 350 199 137
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1401 3594
Size: 1401 2193
TatI w'GTAC_w
Cuts at: 0 478 534 1516 2222 3594
Size: 478 56 982 706 1372
Fragments arranged by size:
1372 982 706 478 56
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 429 461 751 783 1672 1704 2148 2180
Size: 429 32 290 32 889 32 444 32
Cuts at: 2180 2579 2611 3594
Size: 399 32 983
Fragments arranged by size:
983 889 444 429 399 290 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 196 3379 3594
Size: 196 3183 215
Fragments arranged by size:
3183 215 196
XbaI T'CTAG_A
Cuts at: 0 489 2574 3594
Size: 489 2085 1020
Fragments arranged by size:
2085 1020 489
XmnI GAAnn'nnTTC
Cuts at: 0 3433 3594
Size: 3433 161
ZraI GAC'GTC
Cuts at: 0 3209 3594
Size: 3209 385
Enzymes that do cut:
AarI AatII AccI AclI AcuI AflII AflIII AleI
AlfI AloI AlwNI ApaLI ApoI AseI AvaI BamHI
BanI BanII BbsI BbvCI BfrBI BglI BglII BlpI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrDI BsrFI BsrGI BssSI BstEII BstXI
BstYI BstZ17I Bsu36I BtgI BtsI DraIII EaeI EarI
EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII
Hin4I HincII HindIII HpaI MmeI MscI MslI MspA1I
NcoI NsiI NspI PflMI PpiI PpuMI PshAI PstI
SapI SfcI SmlI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XmnI ZraI
Enzymes that do not cut:
Acc65I AfeI AgeI AhdI ApaI AscI AsiSI AvrII
BaeI BbeI BcgI BciVI BclI BmgBI BmtI BplI
BsaI BsaBI BsiEI BsiWI BsmBI BsrBI BssHII BstAPI
BstBI BtgZI ClaI CspCI DraI DrdI EagI EcoICRI
FseI FspI FspAI KasI KpnI MfeI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI PacI PasI
PciI PfoI PmeI PmlI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI SwaI XcmI XhoI XmaI