Restriction map for
pF1KB6004
(Linear) (Six-base) MAPSORT of: KIBB6004.seq Check: 2976 from: 1 to: 5934
KIBB6004.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AccI GT'mk_AC
Cuts at: 0 1362 5934
Size: 1362 4572
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2087 3046 3070 3127 3536 3755 3809 5027
Size: 2087 959 24 57 409 219 54 1218
Cuts at: 5027 5285 5702 5726 5934
Size: 258 417 24 208
Fragments arranged by size:
2087 1218 959 417 409 258 219 208
57 54 24 24
AfeI AGC'GCT
Cuts at: 0 1872 5934
Size: 1872 4062
AflIII A'CryG_T
Cuts at: 0 1086 1541 3463 5848 5934
Size: 1086 455 1922 2385 86
Fragments arranged by size:
2385 1922 1086 455 86
AhdI GACnn_n'nnGTC
Cuts at: 0 907 968 1944 5934
Size: 907 61 976 3990
Fragments arranged by size:
3990 976 907 61
AleI CACnn'nnGTG
Cuts at: 0 1278 5934
Size: 1278 4656
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 5251 5934
Size: 5251 683
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 5003 5035 5934
Size: 5003 32 899
Fragments arranged by size:
5003 899 32
AlwNI CAG_nnn'CTG
Cuts at: 0 3349 5005 5934
Size: 3349 1656 929
Fragments arranged by size:
3349 1656 929
ApoI r'AATT_y
Cuts at: 0 149 695 721 1571 1768 2158 2872 3530
Size: 149 546 26 850 197 390 714 658
Cuts at: 3530 3713 3857 3940 4306 4363 4618 4911 4917
Size: 183 144 83 366 57 255 293 6
Cuts at: 4917 5872 5934
Size: 955 62
Fragments arranged by size:
955 850 714 658 546 390 366 293
255 197 183 149 144 83 62 57
26 6
AseI AT'TA_AT
Cuts at: 0 514 1021 1070 4942 5934
Size: 514 507 49 3872 992
Fragments arranged by size:
3872 992 514 507 49
AvaI C'yCGr_G
Cuts at: 0 23 5934
Size: 23 5911
AvrII C'CTAG_G
Cuts at: 0 1477 5934
Size: 1477 4457
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3833 3866 5934
Size: 3833 33 2068
Fragments arranged by size:
3833 2068 33
BamHI G'GATC_C
Cuts at: 0 1049 5934
Size: 1049 4885
BanI G'GyrC_C
Cuts at: 0 548 5175 5934
Size: 548 4627 759
Fragments arranged by size:
4627 759 548
BbeI G_GCGC'C
Cuts at: 0 552 5934
Size: 552 5382
BbsI GAAGACnn'nnnn_
Cuts at: 0 461 803 1461 3883 5189 5282 5707 5714
Size: 461 342 658 2422 1306 93 425 7
Cuts at: 5714 5934
Size: 220
Fragments arranged by size:
2422 1306 658 461 425 342 220 93
7
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3574 3608 5934
Size: 3574 34 2326
Fragments arranged by size:
3574 2326 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1391 5934
Size: 1391 4543
BfrBI ATG'CAT
Cuts at: 0 1536 5934
Size: 1536 4398
BglII A'GATC_T
Cuts at: 0 5016 5511 5934
Size: 5016 495 423
Fragments arranged by size:
5016 495 423
BlpI GC'TnA_GC
Cuts at: 0 4532 5934
Size: 4532 1402
Bme1580I G_kGCm'C
Cuts at: 0 4265 5180 5934
Size: 4265 915 754
Fragments arranged by size:
4265 915 754
BmgBI CAC'GTC
Cuts at: 0 5851 5934
Size: 5851 83
BmrI ACTGGGnnnn_n'
Cuts at: 0 1466 2549 2884 3875 5934
Size: 1466 1083 335 991 2059
Fragments arranged by size:
2059 1466 1083 991 335
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 435 992 5934
Size: 435 557 4942
Fragments arranged by size:
4942 557 435
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 65 1969 5934
Size: 65 1904 3965
Fragments arranged by size:
3965 1904 65
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 405 1485 3491 5934
Size: 405 1080 2006 2443
Fragments arranged by size:
2443 2006 1080 405
BsaI GGTCTCn'nnnn_
Cuts at: 0 118 1151 1526 5934
Size: 118 1033 375 4408
Fragments arranged by size:
4408 1033 375 118
BsaAI yAC'GTr
Cuts at: 0 145 679 5934
Size: 145 534 5255
Fragments arranged by size:
5255 534 145
BsaBI GATnn'nnATC
Cuts at: 0 1391 1599 1605 1611 1889 2906 5934
Size: 1391 208 6 6 278 1017 3028
Fragments arranged by size:
3028 1391 1017 278 208 6 6
BsaHI Gr'CG_yC
Cuts at: 0 549 5734 5934
Size: 549 5185 200
Fragments arranged by size:
5185 549 200
BsaWI w'CCGG_w
Cuts at: 0 3048 5934
Size: 3048 2886
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 129 159 3503 3533 4746 4776 5003 5033
Size: 129 30 3344 30 1213 30 227 30
Cuts at: 5033 5126 5156 5934
Size: 93 30 778
Fragments arranged by size:
3344 1213 778 227 129 93 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 41 3529 3601 3946 4409 4603 4754 5011
Size: 41 3488 72 345 463 194 151 257
Cuts at: 5011 5359 5934
Size: 348 575
Fragments arranged by size:
3488 575 463 348 345 257 194 151
72 41
BseYI C'CCAG_C
Cuts at: 0 129 3397 5934
Size: 129 3268 2537
Fragments arranged by size:
3268 2537 129
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4817 5934
Size: 4817 1117
BsiHKAI G_wGCw'C
Cuts at: 0 57 144 5934
Size: 57 87 5790
Fragments arranged by size:
5790 87 57
BsmI GAATG_Cn'
Cuts at: 0 539 3401 5934
Size: 539 2862 2533
Fragments arranged by size:
2862 2533 539
Bsp1286I G_dGCh'C
Cuts at: 0 57 144 4265 5180 5934
Size: 57 87 4121 915 754
Fragments arranged by size:
4121 915 754 87 57
BspHI T'CATG_A
Cuts at: 0 267 4496 5934
Size: 267 4229 1438
Fragments arranged by size:
4229 1438 267
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2890 3235 5934
Size: 2890 345 2699
Fragments arranged by size:
2890 2699 345
BsrBI CCG'CTC
Cuts at: 0 1047 5934
Size: 1047 4887
BsrDI GCAATG_nn'
Cuts at: 0 2011 4049 5831 5934
Size: 2011 2038 1782 103
Fragments arranged by size:
2038 2011 1782 103
BsrFI r'CCGG_y
Cuts at: 0 305 5934
Size: 305 5629
BsrGI T'GTAC_A
Cuts at: 0 3030 5934
Size: 3030 2904
BssSI C'ACGA_G
Cuts at: 0 50 4766 5934
Size: 50 4716 1168
Fragments arranged by size:
4716 1168 50
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2176 5934
Size: 2176 3758
BstEII G'GTnAC_C
Cuts at: 0 3211 5934
Size: 3211 2723
BstYI r'GATC_y
Cuts at: 0 103 663 1049 1400 1600 1606 1612 3966
Size: 103 560 386 351 200 6 6 2354
Cuts at: 3966 4409 5016 5511 5934
Size: 443 607 495 423
Fragments arranged by size:
2354 607 560 495 443 423 386 351
200 103 6 6
Bsu36I CC'TnA_GG
Cuts at: 0 4205 5747 5771 5934
Size: 4205 1542 24 163
Fragments arranged by size:
4205 1542 163 24
BtsI GCAGTG_nn'
Cuts at: 0 350 1845 4407 5235 5835 5934
Size: 350 1495 2562 828 600 99
Fragments arranged by size:
2562 1495 828 600 350 99
ClaI AT'CG_AT
Cuts at: 0 626 3024 5934
Size: 626 2398 2910
Fragments arranged by size:
2910 2398 626
DraI TTT'AAA
Cuts at: 0 2163 2795 3599 5114 5369 5934
Size: 2163 632 804 1515 255 565
Fragments arranged by size:
2163 1515 804 632 565 255
DrdI GACnn_nn'nnGTC
Cuts at: 0 1370 2292 5934
Size: 1370 922 3642
Fragments arranged by size:
3642 1370 922
EaeI y'GGCC_r
Cuts at: 0 168 5879 5934
Size: 168 5711 55
Fragments arranged by size:
5711 168 55
EarI CTCTTCn'nnn_
Cuts at: 0 1207 3425 4203 4811 5063 5261 5303 5425
Size: 1207 2218 778 608 252 198 42 122
Cuts at: 5425 5621 5718 5809 5934
Size: 196 97 91 125
Fragments arranged by size:
2218 1207 778 608 252 198 196 125
122 97 91 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 5302 5934
Size: 5302 632
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 65 1969 2087 3046 3070 3127 3536 3755
Size: 65 1904 118 959 24 57 409 219
Cuts at: 3755 3809 5027 5285 5702 5726 5934
Size: 54 1218 258 417 24 208
Fragments arranged by size:
1904 1218 959 417 409 258 219 208
118 65 57 54 24 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2741 5026 5934
Size: 2741 2285 908
Fragments arranged by size:
2741 2285 908
EcoRI G'AATT_C
Cuts at: 0 149 3713 3857 5934
Size: 149 3564 144 2077
Fragments arranged by size:
3564 2077 149 144
EcoRV GAT'ATC
Cuts at: 0 2802 5934
Size: 2802 3132
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 610 685 2820 3076 3267 4447 5329 5934
Size: 610 75 2135 256 191 1180 882 605
Fragments arranged by size:
2135 1180 882 610 605 256 191 75
HaeII r_GCGC'y
Cuts at: 0 552 1874 5934
Size: 552 1322 4060
Fragments arranged by size:
4060 1322 552
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 63 95 893 925 954 986 1314 1346
Size: 63 32 798 32 29 32 328 32
Cuts at: 1346 1675 1707 1930 1962 3343 3375 3503 3535
Size: 329 32 223 32 1381 32 128 32
Cuts at: 3535 4478 4510 5263 5295 5644 5676 5835 5867
Size: 943 32 753 32 349 32 159 32
Cuts at: 5867 5934
Size: 67
Fragments arranged by size:
1381 943 798 753 349 329 328 223
159 128 67 63 32 32 32 32
32 32 32 32 32 32 32 32
29
HincII GTy'rAC
Cuts at: 0 933 1363 1375 3224 5934
Size: 933 430 12 1849 2710
Fragments arranged by size:
2710 1849 933 430 12
HindIII A'AGCT_T
Cuts at: 0 1993 2483 4429 5934
Size: 1993 490 1946 1505
Fragments arranged by size:
1993 1946 1505 490
KasI G'GCGC_C
Cuts at: 0 548 5934
Size: 548 5386
MfeI C'AATT_G
Cuts at: 0 3320 5934
Size: 3320 2614
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 724 995 1071 1246 3060 5319 5578 5934
Size: 724 271 76 175 1814 2259 259 356
Fragments arranged by size:
2259 1814 724 356 271 259 175 76
MscI TGG'CCA
Cuts at: 0 170 5881 5934
Size: 170 5711 53
Fragments arranged by size:
5711 170 53
MslI CAynn'nnrTG
Cuts at: 0 1196 1278 5934
Size: 1196 82 4656
Fragments arranged by size:
4656 1196 82
MspA1I CmG'CkG
Cuts at: 0 4831 5934
Size: 4831 1103
NarI GG'CG_CC
Cuts at: 0 549 5934
Size: 549 5385
NdeI CA'TA_TG
Cuts at: 0 3218 5934
Size: 3218 2716
NsiI A_TGCA'T
Cuts at: 0 1538 5934
Size: 1538 4396
NspI r_CATG'y
Cuts at: 0 640 1090 1195 1545 3467 5934
Size: 640 450 105 350 1922 2467
Fragments arranged by size:
2467 1922 640 450 350 105
PciI A'CATG_T
Cuts at: 0 1086 1541 3463 5934
Size: 1086 455 1922 2471
Fragments arranged by size:
2471 1922 1086 455
PflMI CCAn_nnn'nTGG
Cuts at: 0 2056 2788 5934
Size: 2056 732 3146
Fragments arranged by size:
3146 2056 732
PfoI T'CCnGG_A
Cuts at: 0 81 2244 5934
Size: 81 2163 3690
Fragments arranged by size:
3690 2163 81
PmlI CAC'GTG
Cuts at: 0 145 5934
Size: 145 5789
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 5003 5035 5934
Size: 5003 32 899
Fragments arranged by size:
5003 899 32
PsiI TTA'TAA
Cuts at: 0 2038 2971 5934
Size: 2038 933 2963
Fragments arranged by size:
2963 2038 933
PspXI vC'TCGA_Gb
Cuts at: 0 23 5934
Size: 23 5911
PstI C_TGCA'G
Cuts at: 0 4062 5934
Size: 4062 1872
PvuII CAG'CTG
Cuts at: 0 4831 5934
Size: 4831 1103
SalI G'TCGA_C
Cuts at: 0 1361 5934
Size: 1361 4573
SapI GCTCTTCn'nnn_
Cuts at: 0 3425 5063 5303 5934
Size: 3425 1638 240 631
Fragments arranged by size:
3425 1638 631 240
ScaI AGT'ACT
Cuts at: 0 3393 5934
Size: 3393 2541
SfcI C'TryA_G
Cuts at: 0 2402 2423 2753 3782 4058 4217 5204 5934
Size: 2402 21 330 1029 276 159 987 730
Fragments arranged by size:
2402 1029 987 730 330 276 159 21
SfoI GGC'GCC
Cuts at: 0 550 5934
Size: 550 5384
SmlI C'TyrA_G
Cuts at: 0 23 420 1500 3506 5934
Size: 23 397 1080 2006 2428
Fragments arranged by size:
2428 2006 1080 397 23
SnaBI TAC'GTA
Cuts at: 0 679 5934
Size: 679 5255
SpeI A'CTAG_T
Cuts at: 0 4126 4135 5934
Size: 4126 9 1799
Fragments arranged by size:
4126 1799 9
SphI G_CATG'C
Cuts at: 0 640 5934
Size: 640 5294
SspI AAT'ATT
Cuts at: 0 1268 3207 3821 3897 5934
Size: 1268 1939 614 76 2037
Fragments arranged by size:
2037 1939 1268 614 76
StuI AGG'CCT
Cuts at: 0 78 99 5632 5934
Size: 78 21 5533 302
Fragments arranged by size:
5533 302 78 21
StyI C'CwwG_G
Cuts at: 0 1477 2393 2975 4301 5934
Size: 1477 916 582 1326 1633
Fragments arranged by size:
1633 1477 1326 916 582
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 833 1133 1508 5934
Size: 833 300 375 4426
Fragments arranged by size:
4426 833 375 300
TatI w'GTAC_w
Cuts at: 0 390 1743 2062 2189 3030 3069 3391 5845
Size: 390 1353 319 127 841 39 322 2454
Cuts at: 5845 5934
Size: 89
Fragments arranged by size:
2454 1353 841 390 322 319 127 89
39
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 129 161 648 680 2213 2245 4533 4565
Size: 129 32 487 32 1533 32 2288 32
Cuts at: 4565 5934
Size: 1369
Fragments arranged by size:
2288 1533 1369 487 129 32 32 32
32
XbaI T'CTAG_A
Cuts at: 0 1486 1591 2523 3717 5934
Size: 1486 105 932 1194 2217
Fragments arranged by size:
2217 1486 1194 932 105
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 255 4341 5934
Size: 255 4086 1593
Fragments arranged by size:
4086 1593 255
XhoI C'TCGA_G
Cuts at: 0 23 5934
Size: 23 5911
XmnI GAAnn'nnTTC
Cuts at: 0 1458 3530 5934
Size: 1458 2072 2404
Fragments arranged by size:
2404 2072 1458
Enzymes that do cut:
AccI AcuI AfeI AflIII AhdI AleI AlfI AloI
AlwNI ApoI AseI AvaI AvrII BaeI BamHI BanI
BbeI BbsI BcgI BciVI BfrBI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstEII BstYI Bsu36I BtsI ClaI DraI DrdI
EaeI EarI EciI Eco57MI EcoNI EcoRI EcoRV FalI
HaeII Hin4I HincII HindIII KasI MfeI MmeI MscI
MslI MspA1I NarI NdeI NsiI NspI PciI PflMI
PfoI PmlI PpiI PsiI PspXI PstI PvuII SalI
SapI ScaI SfcI SfoI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI XbaI XcmI
XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI ApaI ApaLI
AscI AsiSI BanII BbvCI BclI BglI BmtI Bpu10I
BsiEI BsiWI BsmBI BspEI BssHII BstBI BstXI BstZ17I
BtgI BtgZI CspCI DraIII EagI EcoICRI EcoO109I FseI
FspI FspAI HpaI KpnI MluI NaeI NcoI NgoMIV
NheI NotI NruI PacI PasI PmeI PpuMI PshAI
PspOMI PsrI PvuI RsrII SacI SacII SanDI SbfI
SexAI SfiI SgfI SgrAI SmaI SrfI SwaI Tth111I
XmaI ZraI