Restriction map for
pF1KB6005
(Linear) (Six-base) MAPSORT of: KIBB6005.seq Check: 2420 from: 1 to: 3249
KIBB6005.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2128 3249
Size: 2128 1121
AatII G_ACGT'C
Cuts at: 0 2864 3249
Size: 2864 385
AccI GT'mk_AC
Cuts at: 0 322 1612 3249
Size: 322 1290 1637
Fragments arranged by size:
1637 1290 322
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 236 533 1089 1154 1789 1838 1862 3205
Size: 236 297 556 65 635 49 24 1343
Cuts at: 3205 3249
Size: 44
Fragments arranged by size:
1343 635 556 297 236 65 49 44
24
AflII C'TTAA_G
Cuts at: 0 3154 3249
Size: 3154 95
AflIII A'CryG_T
Cuts at: 0 2165 3249
Size: 2165 1084
AleI CACnn'nnGTG
Cuts at: 0 1120 1885 2954 3249
Size: 1120 765 1069 295
Fragments arranged by size:
1120 1069 765 295
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2027 2059 3249
Size: 2027 32 1190
Fragments arranged by size:
2027 1190 32
AlwNI CAG_nnn'CTG
Cuts at: 0 706 1126 2401 2497 3249
Size: 706 420 1275 96 752
Fragments arranged by size:
1275 752 706 420 96
ApaI G_GGCC'C
Cuts at: 0 7 662 2434 3249
Size: 7 655 1772 815
Fragments arranged by size:
1772 815 655 7
ApoI r'AATT_y
Cuts at: 0 1769 3249
Size: 1769 1480
AvaI C'yCGr_G
Cuts at: 0 263 692 2090 3249
Size: 263 429 1398 1159
Fragments arranged by size:
1398 1159 429 263
AvrII C'CTAG_G
Cuts at: 0 2128 3249
Size: 2128 1121
BamHI G'GATC_C
Cuts at: 0 1331 1679 3249
Size: 1331 348 1570
Fragments arranged by size:
1570 1331 348
BanI G'GyrC_C
Cuts at: 0 14 1299 2140 2590 3249
Size: 14 1285 841 450 659
Fragments arranged by size:
1285 841 659 450 14
BanII G_rGCy'C
Cuts at: 0 7 111 662 894 1990 2434 3249
Size: 7 104 551 232 1096 444 815
Fragments arranged by size:
1096 815 551 444 232 104 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 2692 3249
Size: 2692 557
BbvCI CC'TCA_GC
Cuts at: 0 1835 2066 3249
Size: 1835 231 1183
Fragments arranged by size:
1835 1183 231
BciVI GTATCCnnnnn_n'
Cuts at: 0 1559 1720 1733 2217 2931 3249
Size: 1559 161 13 484 714 318
Fragments arranged by size:
1559 714 484 318 161 13
BclI T'GATC_A
Cuts at: 0 2223 3249
Size: 2223 1026
BfrBI ATG'CAT
Cuts at: 0 1654 3249
Size: 1654 1595
BlpI GC'TnA_GC
Cuts at: 0 124 782 3249
Size: 124 658 2467
Fragments arranged by size:
2467 658 124
Bme1580I G_kGCm'C
Cuts at: 0 7 662 1304 2434 3249
Size: 7 655 642 1130 815
Fragments arranged by size:
1130 815 655 642 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 587 1002 1279 2767 2958 3249
Size: 587 415 277 1488 191 291
Fragments arranged by size:
1488 587 415 291 277 191
BmtI G_CTAG'C
Cuts at: 0 1967 3249
Size: 1967 1282
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 884 1907 2360 3249
Size: 241 643 1023 453 889
Fragments arranged by size:
1023 889 643 453 241
Bpu10I CC'TnA_GC
Cuts at: 0 1562 1835 2066 3249
Size: 1562 273 231 1183
Fragments arranged by size:
1562 1183 273 231
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 527 2918 3249
Size: 527 2391 331
Fragments arranged by size:
2391 527 331
BsaAI yAC'GTr
Cuts at: 0 2166 3249
Size: 2166 1083
BsaBI GATnn'nnATC
Cuts at: 0 1372 3062 3249
Size: 1372 1690 187
Fragments arranged by size:
1690 1372 187
BsaHI Gr'CG_yC
Cuts at: 0 385 1063 2861 3249
Size: 385 678 1798 388
Fragments arranged by size:
1798 678 388 385
BsaWI w'CCGG_w
Cuts at: 0 1378 2012 2181 3249
Size: 1378 634 169 1068
Fragments arranged by size:
1378 1068 634 169
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1191 1221 1959 1989 2514 2544 3249
Size: 1191 30 738 30 525 30 705
Fragments arranged by size:
1191 738 705 525 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 283 1316 1371 2054 2720 2890 3249
Size: 283 1033 55 683 666 170 359
Fragments arranged by size:
1033 683 666 359 283 170 55
BseYI C'CCAG_C
Cuts at: 0 177 1304 2255 3249
Size: 177 1127 951 994
Fragments arranged by size:
1127 994 951 177
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 719 1733 1796 1963 2366 3249
Size: 331 388 1014 63 167 403 883
Fragments arranged by size:
1014 883 403 388 331 167 63
BsiHKAI G_wGCw'C
Cuts at: 0 236 894 2934 3249
Size: 236 658 2040 315
Fragments arranged by size:
2040 658 315 236
Bsp1286I G_dGCh'C
Cuts at: 0 7 111 236 662 894 1304 1990 2434
Size: 7 104 125 426 232 410 686 444
Cuts at: 2434 2934 3249
Size: 500 315
Fragments arranged by size:
686 500 444 426 410 315 232 125
104 7
BspEI T'CCGG_A
Cuts at: 0 1378 2012 3249
Size: 1378 634 1237
Fragments arranged by size:
1378 1237 634
BspHI T'CATG_A
Cuts at: 0 611 2483 3249
Size: 611 1872 766
Fragments arranged by size:
1872 766 611
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 501 608 2031 2128 3026 3249
Size: 501 107 1423 97 898 223
Fragments arranged by size:
1423 898 501 223 107 97
BsrBI CCG'CTC
Cuts at: 0 2344 3249
Size: 2344 905
BsrDI GCAATG_nn'
Cuts at: 0 458 654 2234 2372 2542 3249
Size: 458 196 1580 138 170 707
Fragments arranged by size:
1580 707 458 196 170 138
BsrFI r'CCGG_y
Cuts at: 0 25 1716 3249
Size: 25 1691 1533
Fragments arranged by size:
1691 1533 25
BssSI C'ACGA_G
Cuts at: 0 1013 3249
Size: 1013 2236
BstAPI GCAn_nnn'nTGC
Cuts at: 0 706 1660 3249
Size: 706 954 1589
Fragments arranged by size:
1589 954 706
BstEII G'GTnAC_C
Cuts at: 0 513 2123 3249
Size: 513 1610 1126
Fragments arranged by size:
1610 1126 513
BstXI CCAn_nnnn'nTGG
Cuts at: 0 561 1404 1887 3249
Size: 561 843 483 1362
Fragments arranged by size:
1362 843 561 483
BstYI r'GATC_y
Cuts at: 0 1186 1331 1679 3249
Size: 1186 145 348 1570
Fragments arranged by size:
1570 1186 348 145
Bsu36I CC'TnA_GG
Cuts at: 0 745 3249
Size: 745 2504
BtgI C'CryG_G
Cuts at: 0 10 524 1409 1937 1996 2195 3249
Size: 10 514 885 528 59 199 1054
Fragments arranged by size:
1054 885 528 514 199 59 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1640 1976 3249
Size: 1640 336 1273
Fragments arranged by size:
1640 1273 336
BtsI GCAGTG_nn'
Cuts at: 0 365 398 1865 2687 3249
Size: 365 33 1467 822 562
Fragments arranged by size:
1467 822 562 365 33
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 336 371 1919 1954 3249
Size: 336 35 1548 35 1295
Fragments arranged by size:
1548 1295 336 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 2590 3249
Size: 2590 659
DrdI GACnn_nn'nnGTC
Cuts at: 0 2866 3249
Size: 2866 383
EaeI y'GGCC_r
Cuts at: 0 826 1406 1889 1999 2174 3249
Size: 826 580 483 110 175 1075
Fragments arranged by size:
1075 826 580 483 175 110
EarI CTCTTCn'nnn_
Cuts at: 0 671 3249
Size: 671 2578
EcoICRI GAG'CTC
Cuts at: 0 892 3249
Size: 892 2357
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 236 241 533 884 1089 1154 1789 1838
Size: 236 5 292 351 205 65 635 49
Cuts at: 1838 1862 1907 2360 3205 3249
Size: 24 45 453 845 44
Fragments arranged by size:
845 635 453 351 292 236 205 65
49 45 44 24 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 101 1055 2598 3249
Size: 101 954 1543 651
Fragments arranged by size:
1543 954 651 101
EcoO109I rG'GnC_Cy
Cuts at: 0 4 132 296 659 1730 2431 3249
Size: 4 128 164 363 1071 701 818
Fragments arranged by size:
1071 818 701 363 164 128 4
EcoRI G'AATT_C
Cuts at: 0 1769 3249
Size: 1769 1480
EcoRV GAT'ATC
Cuts at: 0 2481 3249
Size: 2481 768
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2191 2223 2668 2700 3249
Size: 2191 32 445 32 549
Fragments arranged by size:
2191 549 445 32 32
HincII GTy'rAC
Cuts at: 0 323 3249
Size: 323 2926
HindIII A'AGCT_T
Cuts at: 0 545 3249
Size: 545 2704
MfeI C'AATT_G
Cuts at: 0 1883 3249
Size: 1883 1366
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1566 2272 2302 3249
Size: 1566 706 30 947
Fragments arranged by size:
1566 947 706 30
MscI TGG'CCA
Cuts at: 0 1408 1891 2176 3249
Size: 1408 483 285 1073
Fragments arranged by size:
1408 1073 483 285
MslI CAynn'nnrTG
Cuts at: 0 559 1120 1885 2954 3249
Size: 559 561 765 1069 295
Fragments arranged by size:
1069 765 561 559 295
MspA1I CmG'CkG
Cuts at: 0 12 181 703 762 919 1183 2380 2976
Size: 12 169 522 59 157 264 1197 596
Cuts at: 2976 3249
Size: 273
Fragments arranged by size:
1197 596 522 273 264 169 157 59
12
NaeI GCC'GGC
Cuts at: 0 27 1718 3249
Size: 27 1691 1531
Fragments arranged by size:
1691 1531 27
NcoI C'CATG_G
Cuts at: 0 524 1409 2195 3249
Size: 524 885 786 1054
Fragments arranged by size:
1054 885 786 524
NgoMIV G'CCGG_C
Cuts at: 0 25 1716 3249
Size: 25 1691 1533
Fragments arranged by size:
1691 1533 25
NheI G'CTAG_C
Cuts at: 0 1963 3249
Size: 1963 1286
NsiI A_TGCA'T
Cuts at: 0 1656 3249
Size: 1656 1593
NspI r_CATG'y
Cuts at: 0 928 1663 2629 3249
Size: 928 735 966 620
Fragments arranged by size:
966 928 735 620
PasI CC'CwG_GG
Cuts at: 0 1704 1735 1983 3249
Size: 1704 31 248 1266
Fragments arranged by size:
1704 1266 248 31
PfoI T'CCnGG_A
Cuts at: 0 2337 3249
Size: 2337 912
PmlI CAC'GTG
Cuts at: 0 2166 3249
Size: 2166 1083
PpuMI rG'GwC_Cy
Cuts at: 0 1730 3249
Size: 1730 1519
PspOMI G'GGCC_C
Cuts at: 0 3 658 2430 3249
Size: 3 655 1772 819
Fragments arranged by size:
1772 819 655 3
PspXI vC'TCGA_Gb
Cuts at: 0 692 2090 3249
Size: 692 1398 1159
Fragments arranged by size:
1398 1159 692
PstI C_TGCA'G
Cuts at: 0 984 1131 2139 2502 2897 3023 3249
Size: 984 147 1008 363 395 126 226
Fragments arranged by size:
1008 984 395 363 226 147 126
PvuII CAG'CTG
Cuts at: 0 181 703 762 1183 2380 2976 3249
Size: 181 522 59 421 1197 596 273
Fragments arranged by size:
1197 596 522 421 273 181 59
SacI G_AGCT'C
Cuts at: 0 894 3249
Size: 894 2355
SacII CC_GC'GG
Cuts at: 0 13 3249
Size: 13 3236
SalI G'TCGA_C
Cuts at: 0 321 3249
Size: 321 2928
SanDI GG'GwC_CC
Cuts at: 0 1730 3249
Size: 1730 1519
SbfI CC_TGCA'GG
Cuts at: 0 2139 3023 3249
Size: 2139 884 226
Fragments arranged by size:
2139 884 226
ScaI AGT'ACT
Cuts at: 0 1110 3249
Size: 1110 2139
SexAI A'CCwGG_T
Cuts at: 0 2586 3249
Size: 2586 663
SfcI C'TryA_G
Cuts at: 0 67 185 980 1127 2135 2498 2893 2971
Size: 67 118 795 147 1008 363 395 78
Cuts at: 2971 3019 3249
Size: 48 230
Fragments arranged by size:
1008 795 395 363 230 147 118 78
67 48
SmaI CCC'GGG
Cuts at: 0 265 3249
Size: 265 2984
SmlI C'TyrA_G
Cuts at: 0 542 692 2090 2933 3154 3249
Size: 542 150 1398 843 221 95
Fragments arranged by size:
1398 843 542 221 150 95
SphI G_CATG'C
Cuts at: 0 928 1663 3249
Size: 928 735 1586
Fragments arranged by size:
1586 928 735
SspI AAT'ATT
Cuts at: 0 1459 3249
Size: 1459 1790
StuI AGG'CCT
Cuts at: 0 2133 3249
Size: 2133 1116
StyI C'CwwG_G
Cuts at: 0 497 524 1292 1409 2128 2195 2599 2735
Size: 497 27 768 117 719 67 404 136
Cuts at: 2735 3249
Size: 514
Fragments arranged by size:
768 719 514 497 404 136 117 67
27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1707 2376 2395 3249
Size: 1707 669 19 854
Fragments arranged by size:
1707 854 669 19
TatI w'GTAC_w
Cuts at: 0 792 1108 3249
Size: 792 316 2141
Fragments arranged by size:
2141 792 316
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 226 258 1191 1223 1959 1991 2514 2546
Size: 226 32 933 32 736 32 523 32
Cuts at: 2546 3249
Size: 703
Fragments arranged by size:
933 736 703 523 226 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 3178 3249
Size: 3178 71
XbaI T'CTAG_A
Cuts at: 0 2985 3249
Size: 2985 264
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2034 2700 3249
Size: 2034 666 549
Fragments arranged by size:
2034 666 549
XhoI C'TCGA_G
Cuts at: 0 692 2090 3249
Size: 692 1398 1159
Fragments arranged by size:
1398 1159 692
XmaI C'CCGG_G
Cuts at: 0 263 3249
Size: 263 2986
XmnI GAAnn'nnTTC
Cuts at: 0 1851 3249
Size: 1851 1398
ZraI GAC'GTC
Cuts at: 0 2862 3249
Size: 2862 387
Enzymes that do cut:
AarI AatII AccI AcuI AflII AflIII AleI AloI
AlwNI ApaI ApoI AvaI AvrII BamHI BanI BanII
BbsI BbvCI BciVI BclI BfrBI BlpI Bme1580I BmrI
BmtI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII DrdI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
Hin4I HincII HindIII MfeI MmeI MscI MslI MspA1I
NaeI NcoI NgoMIV NheI NsiI NspI PasI PfoI
PmlI PpuMI PspOMI PspXI PstI PvuII SacI SacII
SalI SanDI SbfI ScaI SexAI SfcI SmaI SmlI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AgeI AhdI AlfI ApaLI AscI
AseI AsiSI BaeI BbeI BcgI BglI BglII BmgBI
BplI BsaI BsiEI BsiWI BsmI BsmBI BsrGI BssHII
BstBI BstZ17I ClaI DraI EagI EciI FalI FseI
FspI FspAI HaeII HpaI KasI KpnI MluI NarI
NdeI NotI NruI PacI PciI PflMI PmeI PpiI
PshAI PsiI PsrI PvuI RsrII SapI SfiI SfoI
SgfI SgrAI SnaBI SpeI SrfI SwaI