Restriction map for
pF1KB6007
(Linear) (Six-base) MAPSORT of: KIBB6007.seq Check: 7209 from: 1 to: 2502
KIBB6007.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
AatII G_ACGT'C
Cuts at: 0 1418 1525 2502
Size: 1418 107 977
Fragments arranged by size:
1418 977 107
AccI GT'mk_AC
Cuts at: 0 863 2054 2502
Size: 863 1191 448
Fragments arranged by size:
1191 863 448
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 510 727 1207 2502
Size: 510 217 480 1295
Fragments arranged by size:
1295 510 480 217
AfeI AGC'GCT
Cuts at: 0 1396 1774 2502
Size: 1396 378 728
Fragments arranged by size:
1396 728 378
AflIII A'CryG_T
Cuts at: 0 878 1931 2502
Size: 878 1053 571
Fragments arranged by size:
1053 878 571
AleI CACnn'nnGTG
Cuts at: 0 1429 2020 2502
Size: 1429 591 482
Fragments arranged by size:
1429 591 482
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1060 1664 2502
Size: 1060 604 838
Fragments arranged by size:
1060 838 604
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 316 348 725 757 2502
Size: 316 32 377 32 1745
Fragments arranged by size:
1745 377 316 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 418 955 2502
Size: 418 537 1547
Fragments arranged by size:
1547 537 418
ApaI G_GGCC'C
Cuts at: 0 1761 2502
Size: 1761 741
ApaLI G'TGCA_C
Cuts at: 0 379 2502
Size: 379 2123
ApoI r'AATT_y
Cuts at: 0 203 721 1627 1788 1825 2502
Size: 203 518 906 161 37 677
Fragments arranged by size:
906 677 518 203 161 37
AvaI C'yCGr_G
Cuts at: 0 81 800 1689 2502
Size: 81 719 889 813
Fragments arranged by size:
889 813 719 81
BamHI G'GATC_C
Cuts at: 0 740 2502
Size: 740 1762
BanI G'GyrC_C
Cuts at: 0 1044 2100 2502
Size: 1044 1056 402
Fragments arranged by size:
1056 1044 402
BanII G_rGCy'C
Cuts at: 0 23 844 944 1761 2502
Size: 23 821 100 817 741
Fragments arranged by size:
821 817 741 100 23
BbeI G_GCGC'C
Cuts at: 0 2104 2502
Size: 2104 398
BbsI GAAGACnn'nnnn_
Cuts at: 0 21 194 550 568 662 878 1302 2416
Size: 21 173 356 18 94 216 424 1114
Cuts at: 2416 2502
Size: 86
Fragments arranged by size:
1114 424 356 216 173 94 86 21
18
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1857 1891 2502
Size: 1857 34 611
Fragments arranged by size:
1857 611 34
BclI T'GATC_A
Cuts at: 0 1748 2502
Size: 1748 754
BfrBI ATG'CAT
Cuts at: 0 1167 2502
Size: 1167 1335
BlpI GC'TnA_GC
Cuts at: 0 397 2502
Size: 397 2105
Bme1580I G_kGCm'C
Cuts at: 0 383 1761 2502
Size: 383 1378 741
Fragments arranged by size:
1378 741 383
BmgBI CAC'GTC
Cuts at: 0 660 2502
Size: 660 1842
BmrI ACTGGGnnnn_n'
Cuts at: 0 428 570 2379 2502
Size: 428 142 1809 123
Fragments arranged by size:
1809 428 142 123
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 703 2502
Size: 703 1799
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 778 1924 2251 2502
Size: 406 372 1146 327 251
Fragments arranged by size:
1146 406 372 327 251
Bpu10I CC'TnA_GC
Cuts at: 0 2074 2502
Size: 2074 428
BsaI GGTCTCn'nnnn_
Cuts at: 0 1138 1694 2317 2476 2502
Size: 1138 556 623 159 26
Fragments arranged by size:
1138 623 556 159 26
BsaAI yAC'GTr
Cuts at: 0 348 879 2502
Size: 348 531 1623
Fragments arranged by size:
1623 531 348
BsaBI GATnn'nnATC
Cuts at: 0 49 2502
Size: 49 2453
BsaHI Gr'CG_yC
Cuts at: 0 1126 1415 1522 2101 2502
Size: 1126 289 107 579 401
Fragments arranged by size:
1126 579 401 289 107
BsaWI w'CCGG_w
Cuts at: 0 257 2502
Size: 257 2245
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 204 234 826 856 931 961 1897 1927
Size: 204 30 592 30 75 30 936 30
Cuts at: 1927 2502
Size: 575
Fragments arranged by size:
936 592 575 204 75 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 148 664 1216 1921 1924 2302 2502
Size: 148 516 552 705 3 378 200
Fragments arranged by size:
705 552 516 378 200 148 3
BseYI C'CCAG_C
Cuts at: 0 2148 2353 2502
Size: 2148 205 149
Fragments arranged by size:
2148 205 149
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 96 388 2118 2502
Size: 96 292 1730 384
Fragments arranged by size:
1730 384 292 96
BsiEI CG_ry'CG
Cuts at: 0 1004 1349 2502
Size: 1004 345 1153
Fragments arranged by size:
1153 1004 345
BsiHKAI G_wGCw'C
Cuts at: 0 383 2381 2435 2502
Size: 383 1998 54 67
Fragments arranged by size:
1998 383 67 54
BsmI GAATG_Cn'
Cuts at: 0 699 1868 2502
Size: 699 1169 634
Fragments arranged by size:
1169 699 634
BsmBI CGTCTCn'nnnn_
Cuts at: 0 242 2179 2502
Size: 242 1937 323
Fragments arranged by size:
1937 323 242
Bsp1286I G_dGCh'C
Cuts at: 0 23 383 844 944 1761 2381 2435 2502
Size: 23 360 461 100 817 620 54 67
Fragments arranged by size:
817 620 461 360 100 67 54 23
BspEI T'CCGG_A
Cuts at: 0 257 2502
Size: 257 2245
BspHI T'CATG_A
Cuts at: 0 1745 2502
Size: 1745 757
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 923 1181 2502
Size: 923 258 1321
Fragments arranged by size:
1321 923 258
BsrBI CCG'CTC
Cuts at: 0 2078 2502
Size: 2078 424
BsrDI GCAATG_nn'
Cuts at: 0 493 2502
Size: 493 2009
BsrGI T'GTAC_A
Cuts at: 0 1964 2216 2502
Size: 1964 252 286
Fragments arranged by size:
1964 286 252
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1173 2502
Size: 1173 1329
BstEII G'GTnAC_C
Cuts at: 0 603 2502
Size: 603 1899
BstXI CCAn_nnnn'nTGG
Cuts at: 0 156 1155 2502
Size: 156 999 1347
Fragments arranged by size:
1347 999 156
BstYI r'GATC_y
Cuts at: 0 740 1674 2502
Size: 740 934 828
Fragments arranged by size:
934 828 740
BstZ17I GTA'TAC
Cuts at: 0 864 2502
Size: 864 1638
Bsu36I CC'TnA_GG
Cuts at: 0 1450 2502
Size: 1450 1052
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1047 2334 2502
Size: 1047 1287 168
Fragments arranged by size:
1287 1047 168
BtsI GCAGTG_nn'
Cuts at: 0 1424 1436 1898 2502
Size: 1424 12 462 604
Fragments arranged by size:
1424 604 462 12
DrdI GACnn_nn'nnGTC
Cuts at: 0 421 1414 2502
Size: 421 993 1088
Fragments arranged by size:
1088 993 421
EaeI y'GGCC_r
Cuts at: 0 284 1001 1388 2079 2502
Size: 284 717 387 691 423
Fragments arranged by size:
717 691 423 387 284
EagI C'GGCC_G
Cuts at: 0 1001 2502
Size: 1001 1501
EarI CTCTTCn'nnn_
Cuts at: 0 550 1027 1492 1692 1792 2212 2502
Size: 550 477 465 200 100 420 290
Fragments arranged by size:
550 477 465 420 290 200 100
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2055 2502
Size: 2055 447
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 510 727 778 1207 1924 2251 2502
Size: 406 104 217 51 429 717 327 251
Fragments arranged by size:
717 429 406 327 251 217 104 51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 127 973 1378 2502
Size: 127 846 405 1124
Fragments arranged by size:
1124 846 405 127
EcoO109I rG'GnC_Cy
Cuts at: 0 465 520 912 1092 1757 2502
Size: 465 55 392 180 665 745
Fragments arranged by size:
745 665 465 392 180 55
EcoRI G'AATT_C
Cuts at: 0 721 1627 2502
Size: 721 906 875
Fragments arranged by size:
906 875 721
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 318 654 1686 2502
Size: 318 336 1032 816
Fragments arranged by size:
1032 816 336 318
HaeII r_GCGC'y
Cuts at: 0 1398 1776 2046 2104 2502
Size: 1398 378 270 58 398
Fragments arranged by size:
1398 398 378 270 58
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 204 236 343 375 555 587 826 858
Size: 204 32 107 32 180 32 239 32
Cuts at: 858 931 963 1897 1929 2475 2502
Size: 73 32 934 32 546 27
Fragments arranged by size:
934 546 239 204 180 107 73 32
32 32 32 32 32 27
HincII GTy'rAC
Cuts at: 0 684 2502
Size: 684 1818
KasI G'GCGC_C
Cuts at: 0 2100 2502
Size: 2100 402
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2132 2502
Size: 2132 370
MscI TGG'CCA
Cuts at: 0 286 2502
Size: 286 2216
MslI CAynn'nnrTG
Cuts at: 0 1429 2020 2502
Size: 1429 591 482
Fragments arranged by size:
1429 591 482
MspA1I CmG'CkG
Cuts at: 0 339 1011 2163 2502
Size: 339 672 1152 339
Fragments arranged by size:
1152 672 339 339
NarI GG'CG_CC
Cuts at: 0 2101 2502
Size: 2101 401
NsiI A_TGCA'T
Cuts at: 0 1169 2502
Size: 1169 1333
NspI r_CATG'y
Cuts at: 0 1180 1935 2502
Size: 1180 755 567
Fragments arranged by size:
1180 755 567
PciI A'CATG_T
Cuts at: 0 1931 2502
Size: 1931 571
PflMI CCAn_nnn'nTGG
Cuts at: 0 969 2000 2502
Size: 969 1031 502
Fragments arranged by size:
1031 969 502
PfoI T'CCnGG_A
Cuts at: 0 1376 1400 1525 2502
Size: 1376 24 125 977
Fragments arranged by size:
1376 977 125 24
PmlI CAC'GTG
Cuts at: 0 348 879 2502
Size: 348 531 1623
Fragments arranged by size:
1623 531 348
PpuMI rG'GwC_Cy
Cuts at: 0 520 912 1092 2502
Size: 520 392 180 1410
Fragments arranged by size:
1410 520 392 180
PshAI GACnn'nnGTC
Cuts at: 0 2302 2502
Size: 2302 200
PspOMI G'GGCC_C
Cuts at: 0 1757 2502
Size: 1757 745
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 847 879 2284 2316 2502
Size: 847 32 1405 32 186
Fragments arranged by size:
1405 847 186 32 32
PstI C_TGCA'G
Cuts at: 0 344 1431 2502
Size: 344 1087 1071
Fragments arranged by size:
1087 1071 344
PvuII CAG'CTG
Cuts at: 0 339 2163 2502
Size: 339 1824 339
Fragments arranged by size:
1824 339 339
SapI GCTCTTCn'nnn_
Cuts at: 0 1027 2502
Size: 1027 1475
SexAI A'CCwGG_T
Cuts at: 0 328 2502
Size: 328 2174
SfcI C'TryA_G
Cuts at: 0 340 615 621 1427 1863 2094 2502
Size: 340 275 6 806 436 231 408
Fragments arranged by size:
806 436 408 340 275 231 6
SfoI GGC'GCC
Cuts at: 0 2102 2502
Size: 2102 400
SmaI CCC'GGG
Cuts at: 0 83 802 2502
Size: 83 719 1700
Fragments arranged by size:
1700 719 83
StuI AGG'CCT
Cuts at: 0 2248 2502
Size: 2248 254
StyI C'CwwG_G
Cuts at: 0 1761 1994 2502
Size: 1761 233 508
Fragments arranged by size:
1761 508 233
TatI w'GTAC_w
Cuts at: 0 1964 2216 2502
Size: 1964 252 286
Fragments arranged by size:
1964 286 252
Tth111I GACn'n_nGTC
Cuts at: 0 1306 2502
Size: 1306 1196
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 510 1983 2160 2502
Size: 510 1473 177 342
Fragments arranged by size:
1473 510 342 177
XmaI C'CCGG_G
Cuts at: 0 81 800 2502
Size: 81 719 1702
Fragments arranged by size:
1702 719 81
XmnI GAAnn'nnTTC
Cuts at: 0 1666 2502
Size: 1666 836
ZraI GAC'GTC
Cuts at: 0 1416 1523 2502
Size: 1416 107 979
Fragments arranged by size:
1416 979 107
Enzymes that do cut:
AatII AccI AcuI AfeI AflIII AleI AlfI AloI
AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII
BbeI BbsI BcgI BclI BfrBI BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrGI
BstAPI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI BtsI
DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI HaeII Hin4I HincII KasI MmeI MscI
MslI MspA1I NarI NsiI NspI PciI PflMI PfoI
PmlI PpuMI PshAI PspOMI PsrI PstI PvuII SapI
SexAI SfcI SfoI SmaI StuI StyI TatI Tth111I
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AflII AgeI AhdI AscI AseI
AsiSI AvrII BaeI BbvCI BciVI BglI BglII BmtI
BpuEI BsiWI BsrFI BssHII BssSI BstBI BtgI ClaI
CspCI DraI DraIII EcoICRI EcoRV FseI FspI FspAI
HindIII HpaI KpnI MfeI MluI NaeI NcoI NdeI
NgoMIV NheI NotI NruI PacI PasI PmeI PpiI
PsiI PspXI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SfiI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SspI SwaI TaqII TstI XbaI XhoI