Restriction map for
pF1KB6016
(Linear) (Six-base) MAPSORT of: KIBB6016.seq Check: 2398 from: 1 to: 2823
KIBB6016.orf
With 182 enzymes: SgfI *
October 2, 2008 10:57 ..
Acc65I G'GTAC_C
Cuts at: 0 872 2823
Size: 872 1951
AclI AA'CG_TT
Cuts at: 0 549 2823
Size: 549 2274
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 848 2054 2411 2542 2823
Size: 848 1206 357 131 281
Fragments arranged by size:
1206 848 357 281 131
AflII C'TTAA_G
Cuts at: 0 2785 2823
Size: 2785 38
AflIII A'CryG_T
Cuts at: 0 2242 2823
Size: 2242 581
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1796 2823
Size: 1796 1027
AlwNI CAG_nnn'CTG
Cuts at: 0 40 1702 2312 2823
Size: 40 1662 610 511
Fragments arranged by size:
1662 610 511 40
ApaLI G'TGCA_C
Cuts at: 0 2004 2823
Size: 2004 819
ApoI r'AATT_y
Cuts at: 0 685 927 1183 1361 1460 1666 2150 2580
Size: 685 242 256 178 99 206 484 430
Cuts at: 2580 2823
Size: 243
Fragments arranged by size:
685 484 430 256 243 242 206 178
99
AvaI C'yCGr_G
Cuts at: 0 383 1999 2823
Size: 383 1616 824
Fragments arranged by size:
1616 824 383
BamHI G'GATC_C
Cuts at: 0 1055 2301 2823
Size: 1055 1246 522
Fragments arranged by size:
1246 1055 522
BanI G'GyrC_C
Cuts at: 0 90 872 2664 2823
Size: 90 782 1792 159
Fragments arranged by size:
1792 782 159 90
BanII G_rGCy'C
Cuts at: 0 1589 1651 2823
Size: 1589 62 1172
Fragments arranged by size:
1589 1172 62
BbeI G_GCGC'C
Cuts at: 0 94 2823
Size: 94 2729
BbsI GAAGACnn'nnnn_
Cuts at: 0 381 1994 2823
Size: 381 1613 829
Fragments arranged by size:
1613 829 381
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 44 78 1990 2024 2329 2363 2823
Size: 44 34 1912 34 305 34 460
Fragments arranged by size:
1912 460 305 44 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2368 2823
Size: 2368 455
BclI T'GATC_A
Cuts at: 0 674 2823
Size: 674 2149
BfrBI ATG'CAT
Cuts at: 0 423 2480 2823
Size: 423 2057 343
Fragments arranged by size:
2057 423 343
BglI GCCn_nnn'nGGC
Cuts at: 0 10 52 2823
Size: 10 42 2771
Fragments arranged by size:
2771 42 10
BglII A'GATC_T
Cuts at: 0 1173 2823
Size: 1173 1650
Bme1580I G_kGCm'C
Cuts at: 0 2008 2669 2823
Size: 2008 661 154
Fragments arranged by size:
2008 661 154
BmgBI CAC'GTC
Cuts at: 0 141 2823
Size: 141 2682
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 62 472 2281 2823
Size: 62 410 1809 542
Fragments arranged by size:
1809 542 410 62
Bpu10I CC'TnA_GC
Cuts at: 0 1135 2823
Size: 1135 1688
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1665 2424 2535 2823
Size: 1665 759 111 288
Fragments arranged by size:
1665 759 288 111
BsaI GGTCTCn'nnnn_
Cuts at: 0 2679 2823
Size: 2679 144
BsaAI yAC'GTr
Cuts at: 0 2243 2823
Size: 2243 580
BsaBI GATnn'nnATC
Cuts at: 0 1724 2823
Size: 1724 1099
BsaHI Gr'CG_yC
Cuts at: 0 91 2823
Size: 91 2732
BsaWI w'CCGG_w
Cuts at: 0 1993 2823
Size: 1993 830
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 497 527 977 1007 2823
Size: 497 30 450 30 1816
Fragments arranged by size:
1816 497 450 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1441 2823
Size: 1441 1382
BseYI C'CCAG_C
Cuts at: 0 2305 2823
Size: 2305 518
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 275 1093 1994 2823
Size: 275 818 901 829
Fragments arranged by size:
901 829 818 275
BsiEI CG_ry'CG
Cuts at: 0 81 2823
Size: 81 2742
BsiHKAI G_wGCw'C
Cuts at: 0 1070 1589 1651 2008 2188 2823
Size: 1070 519 62 357 180 635
Fragments arranged by size:
1070 635 519 357 180 62
BsiWI C'GTAC_G
Cuts at: 0 134 2823
Size: 134 2689
Bsp1286I G_dGCh'C
Cuts at: 0 1070 1589 1651 2008 2188 2669 2823
Size: 1070 519 62 357 180 481 154
Fragments arranged by size:
1070 519 481 357 180 154 62
BspEI T'CCGG_A
Cuts at: 0 1993 2823
Size: 1993 830
BspHI T'CATG_A
Cuts at: 0 152 641 2823
Size: 152 489 2182
Fragments arranged by size:
2182 489 152
BsrBI CCG'CTC
Cuts at: 0 10 2823
Size: 10 2813
BsrFI r'CCGG_y
Cuts at: 0 125 2688 2823
Size: 125 2563 135
Fragments arranged by size:
2563 135 125
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2273 2823
Size: 2273 550
BstYI r'GATC_y
Cuts at: 0 1055 1173 2301 2394 2457 2780 2823
Size: 1055 118 1128 93 63 323 43
Fragments arranged by size:
1128 1055 323 118 93 63 43
Bsu36I CC'TnA_GG
Cuts at: 0 2453 2823
Size: 2453 370
BtgI C'CryG_G
Cuts at: 0 26 50 186 1627 2316 2823
Size: 26 24 136 1441 689 507
Fragments arranged by size:
1441 689 507 136 26 24
BtsI GCAGTG_nn'
Cuts at: 0 1300 2006 2283 2823
Size: 1300 706 277 540
Fragments arranged by size:
1300 706 540 277
DraI TTT'AAA
Cuts at: 0 1316 2823
Size: 1316 1507
EaeI y'GGCC_r
Cuts at: 0 2 23 78 763 2802 2823
Size: 2 21 55 685 2039 21
Fragments arranged by size:
2039 685 55 21 21 2
EagI C'GGCC_G
Cuts at: 0 78 2823
Size: 78 2745
EarI CTCTTCn'nnn_
Cuts at: 0 1628 1814 1856 2030 2823
Size: 1628 186 42 174 793
Fragments arranged by size:
1628 793 186 174 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 86 2823
Size: 86 2737
EcoICRI GAG'CTC
Cuts at: 0 1587 1649 2823
Size: 1587 62 1174
Fragments arranged by size:
1587 1174 62
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 62 472 848 2054 2281 2411 2542 2823
Size: 62 410 376 1206 227 130 131 281
Fragments arranged by size:
1206 410 376 281 227 131 130 62
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1285 2823
Size: 1285 1538
EcoO109I rG'GnC_Cy
Cuts at: 0 720 1602 2823
Size: 720 882 1221
Fragments arranged by size:
1221 882 720
EcoRI G'AATT_C
Cuts at: 0 1460 2580 2823
Size: 1460 1120 243
Fragments arranged by size:
1460 1120 243
EcoRV GAT'ATC
Cuts at: 0 168 448 2823
Size: 168 280 2375
Fragments arranged by size:
2375 280 168
HaeII r_GCGC'y
Cuts at: 0 94 2823
Size: 94 2729
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 455 487 497 529 2366 2379 2398 2411
Size: 455 32 10 32 1837 13 19 13
Cuts at: 2411 2823
Size: 412
Fragments arranged by size:
1837 455 412 32 32 19 13 13
10
HincII GTy'rAC
Cuts at: 0 225 2823
Size: 225 2598
KasI G'GCGC_C
Cuts at: 0 90 2823
Size: 90 2733
KpnI G_GTAC'C
Cuts at: 0 876 2823
Size: 876 1947
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 180 1707 2105 2823
Size: 180 1527 398 718
Fragments arranged by size:
1527 718 398 180
MscI TGG'CCA
Cuts at: 0 765 2804 2823
Size: 765 2039 19
Fragments arranged by size:
2039 765 19
MslI CAynn'nnrTG
Cuts at: 0 305 586 626 1126 2823
Size: 305 281 40 500 1697
Fragments arranged by size:
1697 500 305 281 40
MspA1I CmG'CkG
Cuts at: 0 28 40 52 194 435 2312 2546 2823
Size: 28 12 12 142 241 1877 234 277
Fragments arranged by size:
1877 277 241 234 142 28 12 12
NaeI GCC'GGC
Cuts at: 0 127 2823
Size: 127 2696
NarI GG'CG_CC
Cuts at: 0 91 2823
Size: 91 2732
NcoI C'CATG_G
Cuts at: 0 1627 2316 2823
Size: 1627 689 507
Fragments arranged by size:
1627 689 507
NdeI CA'TA_TG
Cuts at: 0 1204 2823
Size: 1204 1619
NgoMIV G'CCGG_C
Cuts at: 0 125 2823
Size: 125 2698
NsiI A_TGCA'T
Cuts at: 0 425 2482 2823
Size: 425 2057 341
Fragments arranged by size:
2057 425 341
NspI r_CATG'y
Cuts at: 0 1131 1354 2214 2823
Size: 1131 223 860 609
Fragments arranged by size:
1131 860 609 223
PflMI CCAn_nnn'nTGG
Cuts at: 0 1208 1915 2823
Size: 1208 707 908
Fragments arranged by size:
1208 908 707
PfoI T'CCnGG_A
Cuts at: 0 2297 2416 2823
Size: 2297 119 407
Fragments arranged by size:
2297 407 119
PmlI CAC'GTG
Cuts at: 0 2243 2823
Size: 2243 580
PpuMI rG'GwC_Cy
Cuts at: 0 720 1602 2823
Size: 720 882 1221
Fragments arranged by size:
1221 882 720
PsiI TTA'TAA
Cuts at: 0 415 2823
Size: 415 2408
PstI C_TGCA'G
Cuts at: 0 2278 2823
Size: 2278 545
PvuII CAG'CTG
Cuts at: 0 40 194 435 2312 2823
Size: 40 154 241 1877 511
Fragments arranged by size:
1877 511 241 154 40
SacI G_AGCT'C
Cuts at: 0 1589 1651 2823
Size: 1589 62 1172
Fragments arranged by size:
1589 1172 62
SacII CC_GC'GG
Cuts at: 0 29 53 2823
Size: 29 24 2770
Fragments arranged by size:
2770 29 24
SfcI C'TryA_G
Cuts at: 0 1046 2274 2823
Size: 1046 1228 549
Fragments arranged by size:
1228 1046 549
SfoI GGC'GCC
Cuts at: 0 92 2823
Size: 92 2731
SmlI C'TyrA_G
Cuts at: 0 383 1644 2403 2550 2785 2823
Size: 383 1261 759 147 235 38
Fragments arranged by size:
1261 759 383 235 147 38
SphI G_CATG'C
Cuts at: 0 1131 2214 2823
Size: 1131 1083 609
Fragments arranged by size:
1131 1083 609
SspI AAT'ATT
Cuts at: 0 805 2823
Size: 805 2018
StyI C'CwwG_G
Cuts at: 0 1627 2316 2823
Size: 1627 689 507
Fragments arranged by size:
1627 689 507
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 738 2823
Size: 738 2085
TatI w'GTAC_w
Cuts at: 0 113 1284 2823
Size: 113 1171 1539
Fragments arranged by size:
1539 1171 113
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 977 1009 2823
Size: 977 32 1814
Fragments arranged by size:
1814 977 32
XbaI T'CTAG_A
Cuts at: 0 2140 2823
Size: 2140 683
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1227 2614 2823
Size: 1227 1387 209
Fragments arranged by size:
1387 1227 209
XhoI C'TCGA_G
Cuts at: 0 383 2823
Size: 383 2440
XmnI GAAnn'nnTTC
Cuts at: 0 148 1563 2201 2823
Size: 148 1415 638 622
Fragments arranged by size:
1415 638 622 148
Enzymes that do cut:
Acc65I AclI AcuI AflII AflIII AlfI AlwNI ApaLI
ApoI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII Bme1580I BmgBI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspEI
BspHI BsrBI BsrFI BstAPI BstYI Bsu36I BtgI BtsI
DraI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV HaeII Hin4I HincII KasI KpnI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NsiI NspI PflMI PfoI PmlI PpuMI PsiI
PstI PvuII SacI SacII SfcI SfoI SmlI SphI
SspI StyI TaqII TatI TstI XbaI XcmI XhoI
XmnI
Enzymes that do not cut:
AarI AatII AccI AfeI AgeI AhdI AleI AloI
ApaI AscI AseI AsiSI AvrII BaeI BbvCI BlpI
BmrI BmtI BplI BsmI BsmBI BspMI BsrDI BsrGI
BssHII BssSI BstBI BstEII BstXI BstZ17I BtgZI ClaI
CspCI DraIII DrdI FalI FseI FspI FspAI HindIII
HpaI MfeI MluI NheI NotI NruI PacI PasI
PciI PmeI PpiI PshAI PspOMI PspXI PsrI PvuI
RsrII SalI SanDI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SnaBI SpeI SrfI StuI SwaI
Tth111I XmaI ZraI