Restriction map for
pF1KB6017
(Linear) (Six-base) MAPSORT of: KIBB6017.seq Check: 2223 from: 1 to: 2481
KIBB6017.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2129 2481
Size: 2129 352
AccI GT'mk_AC
Cuts at: 0 1436 2481
Size: 1436 1045
Acc65I G'GTAC_C
Cuts at: 0 779 2481
Size: 779 1702
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1734 2280 2296 2404 2481
Size: 1734 546 16 108 77
Fragments arranged by size:
1734 546 108 77 16
AfeI AGC'GCT
Cuts at: 0 399 2481
Size: 399 2082
AflIII A'CryG_T
Cuts at: 0 2423 2481
Size: 2423 58
AlwNI CAG_nnn'CTG
Cuts at: 0 193 999 2481
Size: 193 806 1482
Fragments arranged by size:
1482 806 193
ApaI G_GGCC'C
Cuts at: 0 1368 2481
Size: 1368 1113
ApaLI G'TGCA_C
Cuts at: 0 1444 2481
Size: 1444 1037
AvaI C'yCGr_G
Cuts at: 0 832 844 1155 1818 2481
Size: 832 12 311 663 663
Fragments arranged by size:
832 663 663 311 12
BamHI G'GATC_C
Cuts at: 0 1070 2481
Size: 1070 1411
BanI G'GyrC_C
Cuts at: 0 199 274 431 511 652 779 934 1741
Size: 199 75 157 80 141 127 155 807
Cuts at: 1741 2481
Size: 740
Fragments arranged by size:
807 740 199 157 155 141 127 80
75
BanII G_rGCy'C
Cuts at: 0 82 631 1368 1691 1762 2315 2481
Size: 82 549 737 323 71 553 166
Fragments arranged by size:
737 553 549 323 166 82 71
BbeI G_GCGC'C
Cuts at: 0 435 2481
Size: 435 2046
BbsI GAAGACnn'nnnn_
Cuts at: 0 276 1914 1993 2082 2481
Size: 276 1638 79 89 399
Fragments arranged by size:
1638 399 276 89 79
BbvCI CC'TCA_GC
Cuts at: 0 929 2481
Size: 929 1552
BglI GCCn_nnn'nGGC
Cuts at: 0 626 811 1372 2481
Size: 626 185 561 1109
Fragments arranged by size:
1109 626 561 185
BglII A'GATC_T
Cuts at: 0 572 2481
Size: 572 1909
BlpI GC'TnA_GC
Cuts at: 0 352 2365 2481
Size: 352 2013 116
Fragments arranged by size:
2013 352 116
Bme1580I G_kGCm'C
Cuts at: 0 1368 1448 2481
Size: 1368 80 1033
Fragments arranged by size:
1368 1033 80
BmgBI CAC'GTC
Cuts at: 0 1503 2481
Size: 1503 978
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 568 1591 2458 2481
Size: 568 1023 867 23
Fragments arranged by size:
1023 867 568 23
Bpu10I CC'TnA_GC
Cuts at: 0 929 1075 2461 2481
Size: 929 146 1386 20
Fragments arranged by size:
1386 929 146 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 2299 2481
Size: 90 2209 182
Fragments arranged by size:
2209 182 90
BsaI GGTCTCn'nnnn_
Cuts at: 0 46 1843 2481
Size: 46 1797 638
Fragments arranged by size:
1797 638 46
BsaAI yAC'GTr
Cuts at: 0 975 1035 1440 2424 2445 2481
Size: 975 60 405 984 21 36
Fragments arranged by size:
984 975 405 60 36 21
BsaHI Gr'CG_yC
Cuts at: 0 432 1051 1910 2087 2481
Size: 432 619 859 177 394
Fragments arranged by size:
859 619 432 394 177
BsaWI w'CCGG_w
Cuts at: 0 1067 2153 2481
Size: 1067 1086 328
Fragments arranged by size:
1086 1067 328
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2041 2071 2344 2374 2481
Size: 2041 30 273 30 107
Fragments arranged by size:
2041 273 107 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 565 1291 1699 2481
Size: 565 726 408 782
Fragments arranged by size:
782 726 565 408
BseYI C'CCAG_C
Cuts at: 0 83 115 219 375 854 879 999 1026
Size: 83 32 104 156 479 25 120 27
Cuts at: 1026 1253 1536 1713 2172 2481
Size: 227 283 177 459 309
Fragments arranged by size:
479 459 309 283 227 177 156 120
104 83 32 27 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 782 1057 1216 1234 2481
Size: 782 275 159 18 1247
Fragments arranged by size:
1247 782 275 159 18
BsiEI CG_ry'CG
Cuts at: 0 1068 1391 2481
Size: 1068 323 1090
Fragments arranged by size:
1090 1068 323
BsiHKAI G_wGCw'C
Cuts at: 0 1448 1691 1970 2315 2339 2444 2481
Size: 1448 243 279 345 24 105 37
Fragments arranged by size:
1448 345 279 243 105 37 24
BsmI GAATG_Cn'
Cuts at: 0 1389 2481
Size: 1389 1092
BsmBI CGTCTCn'nnnn_
Cuts at: 0 363 2481
Size: 363 2118
Bsp1286I G_dGCh'C
Cuts at: 0 82 631 1368 1448 1691 1762 1970 2315
Size: 82 549 737 80 243 71 208 345
Cuts at: 2315 2339 2444 2481
Size: 24 105 37
Fragments arranged by size:
737 549 345 243 208 105 82 80
71 37 24
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2129 2481
Size: 2129 352
BsrDI GCAATG_nn'
Cuts at: 0 1892 2481
Size: 1892 589
BsrFI r'CCGG_y
Cuts at: 0 767 1134 1411 1507 2481
Size: 767 367 277 96 974
Fragments arranged by size:
974 767 367 277 96
BsrGI T'GTAC_A
Cuts at: 0 2057 2481
Size: 2057 424
BssSI C'ACGA_G
Cuts at: 0 32 313 2481
Size: 32 281 2168
Fragments arranged by size:
2168 281 32
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2343 2481
Size: 2343 138
BstBI TT'CG_AA
Cuts at: 0 1457 2481
Size: 1457 1024
BstEII G'GTnAC_C
Cuts at: 0 1146 2481
Size: 1146 1335
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1261 2112 2481
Size: 1261 851 369
Fragments arranged by size:
1261 851 369
BstYI r'GATC_y
Cuts at: 0 572 1070 2481
Size: 572 498 1411
Fragments arranged by size:
1411 572 498
BstZ17I GTA'TAC
Cuts at: 0 1437 2481
Size: 1437 1044
Bsu36I CC'TnA_GG
Cuts at: 0 807 2481
Size: 807 1674
BtgI C'CryG_G
Cuts at: 0 897 1223 1260 1422 1784 1853 2045 2347
Size: 897 326 37 162 362 69 192 302
Cuts at: 2347 2481
Size: 134
Fragments arranged by size:
897 362 326 302 192 162 134 69
37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 597 909 2481
Size: 597 312 1572
Fragments arranged by size:
1572 597 312
BtsI GCAGTG_nn'
Cuts at: 0 135 722 771 1766 2362 2481
Size: 135 587 49 995 596 119
Fragments arranged by size:
995 596 587 135 119 49
ClaI AT'CG_AT
Cuts at: 0 635 707 2481
Size: 635 72 1774
Fragments arranged by size:
1774 635 72
DraIII CAC_nnn'GTG
Cuts at: 0 1503 2481
Size: 1503 978
DrdI GACnn_nn'nnGTC
Cuts at: 0 1954 2481
Size: 1954 527
EaeI y'GGCC_r
Cuts at: 0 1346 1388 1505 1877 2414 2481
Size: 1346 42 117 372 537 67
Fragments arranged by size:
1346 537 372 117 67 42
EagI C'GGCC_G
Cuts at: 0 1388 2481
Size: 1388 1093
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 577 2481
Size: 577 1904
EcoICRI GAG'CTC
Cuts at: 0 1689 2313 2481
Size: 1689 624 168
Fragments arranged by size:
1689 624 168
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 568 1591 1734 2280 2296 2404 2458 2481
Size: 568 1023 143 546 16 108 54 23
Fragments arranged by size:
1023 568 546 143 108 54 23 16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 316 547 1468 1558 2125 2481
Size: 316 231 921 90 567 356
Fragments arranged by size:
921 567 356 316 231 90
EcoO109I rG'GnC_Cy
Cuts at: 0 108 309 743 902 981 1364 1365 1550
Size: 108 201 434 159 79 383 1 185
Cuts at: 1550 1929 2004 2481
Size: 379 75 477
Fragments arranged by size:
477 434 383 379 201 185 159 108
79 75 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1353 2106 2481
Size: 1353 753 375
Fragments arranged by size:
1353 753 375
HaeII r_GCGC'y
Cuts at: 0 401 435 790 1111 1680 1833 2138 2481
Size: 401 34 355 321 569 153 305 343
Fragments arranged by size:
569 401 355 343 321 305 153 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 899 931 2481
Size: 899 32 1550
Fragments arranged by size:
1550 899 32
HincII GTy'rAC
Cuts at: 0 1959 2481
Size: 1959 522
KasI G'GCGC_C
Cuts at: 0 431 2481
Size: 431 2050
KpnI G_GTAC'C
Cuts at: 0 783 2481
Size: 783 1698
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 386 1787 2164 2180 2481
Size: 386 1401 377 16 301
Fragments arranged by size:
1401 386 377 301 16
MscI TGG'CCA
Cuts at: 0 1348 1879 2481
Size: 1348 531 602
Fragments arranged by size:
1348 602 531
MslI CAynn'nnrTG
Cuts at: 0 268 658 1699 1897 2410 2481
Size: 268 390 1041 198 513 71
Fragments arranged by size:
1041 513 390 268 198 71
MspA1I CmG'CkG
Cuts at: 0 115 375 442 1041 1569 1659 2481
Size: 115 260 67 599 528 90 822
Fragments arranged by size:
822 599 528 260 115 90 67
NaeI GCC'GGC
Cuts at: 0 769 1136 1509 2481
Size: 769 367 373 972
Fragments arranged by size:
972 769 373 367
NarI GG'CG_CC
Cuts at: 0 432 2481
Size: 432 2049
NcoI C'CATG_G
Cuts at: 0 1223 1422 1784 1853 2481
Size: 1223 199 362 69 628
Fragments arranged by size:
1223 628 362 199 69
NgoMIV G'CCGG_C
Cuts at: 0 767 1134 1507 2481
Size: 767 367 373 974
Fragments arranged by size:
974 767 373 367
NspI r_CATG'y
Cuts at: 0 2346 2481
Size: 2346 135
PflMI CCAn_nnn'nTGG
Cuts at: 0 2192 2481
Size: 2192 289
PmlI CAC'GTG
Cuts at: 0 1035 2445 2481
Size: 1035 1410 36
Fragments arranged by size:
1410 1035 36
PpuMI rG'GwC_Cy
Cuts at: 0 743 902 981 1550 1929 2481
Size: 743 159 79 569 379 552
Fragments arranged by size:
743 569 552 379 159 79
PshAI GACnn'nnGTC
Cuts at: 0 2107 2481
Size: 2107 374
PspOMI G'GGCC_C
Cuts at: 0 1364 2481
Size: 1364 1117
PstI C_TGCA'G
Cuts at: 0 142 1658 2101 2126 2481
Size: 142 1516 443 25 355
Fragments arranged by size:
1516 443 355 142 25
PvuII CAG'CTG
Cuts at: 0 375 1569 1659 2481
Size: 375 1194 90 822
Fragments arranged by size:
1194 822 375 90
RsrII CG'GwC_CG
Cuts at: 0 1409 2481
Size: 1409 1072
SacI G_AGCT'C
Cuts at: 0 1691 2315 2481
Size: 1691 624 166
Fragments arranged by size:
1691 624 166
SanDI GG'GwC_CC
Cuts at: 0 902 2481
Size: 902 1579
SexAI A'CCwGG_T
Cuts at: 0 452 983 2481
Size: 452 531 1498
Fragments arranged by size:
1498 531 452
SfcI C'TryA_G
Cuts at: 0 138 584 1654 2097 2122 2481
Size: 138 446 1070 443 25 359
Fragments arranged by size:
1070 446 443 359 138 25
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 811 2481
Size: 811 1670
SfoI GGC'GCC
Cuts at: 0 433 2481
Size: 433 2048
SmlI C'TyrA_G
Cuts at: 0 69 2314 2481
Size: 69 2245 167
Fragments arranged by size:
2245 167 69
SphI G_CATG'C
Cuts at: 0 2346 2481
Size: 2346 135
StuI AGG'CCT
Cuts at: 0 412 1463 1639 2481
Size: 412 1051 176 842
Fragments arranged by size:
1051 842 412 176
StyI C'CwwG_G
Cuts at: 0 104 1205 1223 1322 1422 1469 1559 1784
Size: 104 1101 18 99 100 47 90 225
Cuts at: 1784 1853 2481
Size: 69 628
Fragments arranged by size:
1101 628 225 104 100 99 90 69
47 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 262 871 1267 2481
Size: 262 609 396 1214
Fragments arranged by size:
1214 609 396 262
TatI w'GTAC_w
Cuts at: 0 2057 2066 2481
Size: 2057 9 415
Fragments arranged by size:
2057 415 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2344 2376 2481
Size: 2344 32 105
Fragments arranged by size:
2344 105 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 91 1261 1356 1566 2354 2481
Size: 91 1170 95 210 788 127
Fragments arranged by size:
1170 788 210 127 95 91
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AlwNI ApaI
ApaLI AvaI BamHI BanI BanII BbeI BbsI BbvCI
BglI BglII BlpI Bme1580I BmgBI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI DraIII DrdI EaeI
EagI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I HincII KasI KpnI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PflMI PmlI PpuMI
PshAI PspOMI PstI PvuII RsrII SacI SanDI SexAI
SfcI SfiI SfoI SmlI SphI StuI StyI TaqII
TatI TstI XcmI
Enzymes that do not cut:
AatII AclI AflII AgeI AhdI AleI AlfI AloI
ApoI AscI AseI AsiSI AvrII BaeI BcgI BciVI
BclI BfrBI BmrI BmtI BplI BsaBI BsiWI BspEI
BspHI BsrBI BssHII CspCI DraI EarI EcoRI EcoRV
FseI FspI FspAI HindIII HpaI MfeI MluI NdeI
NheI NotI NruI NsiI PacI PasI PciI PfoI
PmeI PpiI PsiI PspXI PsrI PvuI SacII SalI
SapI SbfI ScaI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI SwaI Tth111I XbaI XhoI XmaI XmnI
ZraI