(Linear) (Six-base) MAPSORT of: KIBB6103.seq  Check: 1381  from: 1  to: 1731



KIBB6103.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AccI GT'mk_AC

Cuts at:      0    1104    1731 
   Size:       1104     627
 
AclI AA'CG_TT

Cuts at:      0     401    1731 
   Size:        401    1330
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     314     416    1385    1731 
   Size:        314     102     969     346

  Fragments arranged by size: 

                 969     346     314     102
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     215     247    1731 
   Size:        215      32    1484

  Fragments arranged by size: 

                1484     215      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     661    1731 
   Size:        661    1070
 
ApaI G_GGCC'C

Cuts at:      0    1602    1731 
   Size:       1602     129
 
ApoI r'AATT_y

Cuts at:      0     469    1056    1135    1469    1612    1731 
   Size:        469     587      79     334     143     119

  Fragments arranged by size: 

                 587     469     334     143     119      79
 
AseI AT'TA_AT

Cuts at:      0     508    1672    1731 
   Size:        508    1164      59

  Fragments arranged by size: 

                1164     508      59
 
BamHI G'GATC_C

Cuts at:      0    1408    1731 
   Size:       1408     323
 
BanII G_rGCy'C

Cuts at:      0    1602    1731 
   Size:       1602     129
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      29    1731 
   Size:         29    1702
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     532     566    1731 
   Size:        532      34    1165

  Fragments arranged by size: 

                1165     532      34
 
Bme1580I G_kGCm'C

Cuts at:      0    1602    1731 
   Size:       1602     129
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1596    1731 
   Size:       1596     135
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55    1731 
   Size:         55    1676
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     527    1731 
   Size:        527    1204
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     107    1217    1277    1731 
   Size:        107    1110      60     454

  Fragments arranged by size: 

                1110     454     107      60
 
BsmI GAATG_Cn'

Cuts at:      0    1567    1731 
   Size:       1567     164
 
Bsp1286I G_dGCh'C

Cuts at:      0    1602    1731 
   Size:       1602     129
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     813    1731 
   Size:        813     918
 
BstBI TT'CG_AA

Cuts at:      0     978    1731 
   Size:        978     753
 
BstYI r'GATC_y

Cuts at:      0    1408    1731 
   Size:       1408     323
 
BstZ17I GTA'TAC

Cuts at:      0    1105    1731 
   Size:       1105     626
 
BtsI GCAGTG_nn'

Cuts at:      0     772    1561    1731 
   Size:        772     789     170

  Fragments arranged by size: 

                 789     772     170
 
DraI TTT'AAA

Cuts at:      0    1140    1731 
   Size:       1140     591
 
DraIII CAC_nnn'GTG

Cuts at:      0      48    1731 
   Size:         48    1683
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     187    1731 
   Size:        187    1544
 
EarI CTCTTCn'nnn_

Cuts at:      0     219     590    1168    1204    1394    1715    1731 
   Size:        219     371     578      36     190     321      16

  Fragments arranged by size: 

                 578     371     321     219     190      36      16
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      55     314     416    1385    1731 
   Size:         55     259     102     969     346

  Fragments arranged by size: 

                 969     346     259     102      55
 
EcoRI G'AATT_C

Cuts at:      0    1056    1731 
   Size:       1056     675
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     621     653    1731 
   Size:        621      32    1078

  Fragments arranged by size: 

                1078     621      32
 
HincII GTy'rAC

Cuts at:      0     132     479    1607    1731 
   Size:        132     347    1128     124

  Fragments arranged by size: 

                1128     347     132     124
 
HindIII A'AGCT_T

Cuts at:      0    1627    1731 
   Size:       1627     104
 
HpaI GTT'AAC

Cuts at:      0    1607    1731 
   Size:       1607     124
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     581    1731 
   Size:        581    1150
 
MslI CAynn'nnrTG

Cuts at:      0    1280    1731 
   Size:       1280     451
 
MspA1I CmG'CkG

Cuts at:      0    1497    1731 
   Size:       1497     234
 
NdeI CA'TA_TG

Cuts at:      0    1085    1731 
   Size:       1085     646
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1182    1731 
   Size:       1182     549
 
PfoI T'CCnGG_A

Cuts at:      0    1029    1731 
   Size:       1029     702
 
PsiI TTA'TAA

Cuts at:      0    1633    1731 
   Size:       1633      98
 
PspOMI G'GGCC_C

Cuts at:      0    1598    1731 
   Size:       1598     133
 
PstI C_TGCA'G

Cuts at:      0     779    1731 
   Size:        779     952
 
PvuII CAG'CTG

Cuts at:      0    1497    1731 
   Size:       1497     234
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1204    1731 
   Size:       1204     527
 
SfcI C'TryA_G

Cuts at:      0     260     775    1731 
   Size:        260     515     956

  Fragments arranged by size: 

                 956     515     260
 
SspI AAT'ATT

Cuts at:      0     547     727    1731 
   Size:        547     180    1004

  Fragments arranged by size: 

                1004     547     180
 
StyI C'CwwG_G

Cuts at:      0     517     918    1731 
   Size:        517     401     813

  Fragments arranged by size: 

                 813     517     401
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1025    1057    1731 
   Size:       1025      32     674

  Fragments arranged by size: 

                1025     674      32
 
XbaI T'CTAG_A

Cuts at:      0      78    1731 
   Size:         78    1653
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1222    1731 
   Size:       1222     509
 
XmnI GAAnn'nnTTC

Cuts at:      0     974    1731 
   Size:        974     757
 

 Enzymes that do cut:

     AccI     AclI     AcuI     AloI    AlwNI     ApaI     ApoI     AseI
    BamHI    BanII     BbsI     BcgI Bme1580I     BmrI     BpmI     BsaI
    BseRI     BsmI Bsp1286I   BstAPI    BstBI    BstYI  BstZ17I     BtsI
     DraI   DraIII     DrdI     EarI  Eco57MI    EcoRI    Hin4I   HincII
  HindIII     HpaI     MmeI     MslI   MspA1I     NdeI    PflMI     PfoI
     PsiI   PspOMI     PstI    PvuII     SapI     SfcI     SspI     StyI
     TstI     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI    ApaLI     AscI    AsiSI     AvaI    AvrII     BaeI
     BanI     BbeI    BbvCI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmtI     BplI   Bpu10I    BpuEI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstEII    BstXI   Bsu36I     BtgI    BtgZI     ClaI
    CspCI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV
     FalI     FseI     FspI    FspAI    HaeII     KasI     KpnI     MfeI
     MluI     MscI     NaeI     NarI     NcoI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI     PmeI     PmlI
     PpiI    PpuMI    PshAI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     StuI     SwaI    TaqII     TatI  Tth111I     XhoI     XmaI     ZraI