Restriction map for
pF1KB6110
(Linear) (Six-base) MAPSORT of: KIBB6110.seq Check: 3204 from: 1 to: 1884
KIBB6110.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1033 1043 1560 1884
Size: 1033 10 517 324
Fragments arranged by size:
1033 517 324 10
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 1588 1884
Size: 154 1434 296
Fragments arranged by size:
1434 296 154
AflIII A'CryG_T
Cuts at: 0 1093 1884
Size: 1093 791
AhdI GACnn_n'nnGTC
Cuts at: 0 1613 1856 1884
Size: 1613 243 28
Fragments arranged by size:
1613 243 28
AleI CACnn'nnGTG
Cuts at: 0 772 975 1884
Size: 772 203 909
Fragments arranged by size:
909 772 203
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 857 1884
Size: 857 1027
AlwNI CAG_nnn'CTG
Cuts at: 0 757 1884
Size: 757 1127
ApaI G_GGCC'C
Cuts at: 0 215 325 1714 1884
Size: 215 110 1389 170
Fragments arranged by size:
1389 215 170 110
ApaLI G'TGCA_C
Cuts at: 0 367 1884
Size: 367 1517
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1528 1561 1884
Size: 1528 33 323
Fragments arranged by size:
1528 323 33
BamHI G'GATC_C
Cuts at: 0 1207 1884
Size: 1207 677
BanI G'GyrC_C
Cuts at: 0 247 310 1214 1758 1884
Size: 247 63 904 544 126
Fragments arranged by size:
904 544 247 126 63
BanII G_rGCy'C
Cuts at: 0 56 110 215 325 422 704 1492 1714
Size: 56 54 105 110 97 282 788 222
Cuts at: 1714 1738 1884
Size: 24 146
Fragments arranged by size:
788 282 222 146 110 105 97 56
54 24
BbeI G_GCGC'C
Cuts at: 0 1762 1884
Size: 1762 122
BbsI GAAGACnn'nnnn_
Cuts at: 0 208 1884
Size: 208 1676
BbvCI CC'TCA_GC
Cuts at: 0 1413 1884
Size: 1413 471
BciVI GTATCCnnnnn_n'
Cuts at: 0 889 1884
Size: 889 995
BclI T'GATC_A
Cuts at: 0 908 1884
Size: 908 976
BglI GCCn_nnn'nGGC
Cuts at: 0 708 1426 1756 1884
Size: 708 718 330 128
Fragments arranged by size:
718 708 330 128
Bme1580I G_kGCm'C
Cuts at: 0 150 215 325 371 661 906 982 1020
Size: 150 65 110 46 290 245 76 38
Cuts at: 1020 1219 1504 1714 1884
Size: 199 285 210 170
Fragments arranged by size:
290 285 245 210 199 170 150 110
76 65 46 38
BmrI ACTGGGnnnn_n'
Cuts at: 0 1288 1573 1884
Size: 1288 285 311
Fragments arranged by size:
1288 311 285
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 1267 1884
Size: 40 1227 617
Fragments arranged by size:
1227 617 40
Bpu10I CC'TnA_GC
Cuts at: 0 574 1413 1483 1884
Size: 574 839 70 401
Fragments arranged by size:
839 574 401 70
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1827 1884
Size: 1827 57
BsaI GGTCTCn'nnnn_
Cuts at: 0 586 1884
Size: 586 1298
BsaBI GATnn'nnATC
Cuts at: 0 166 1884
Size: 166 1718
BsaHI Gr'CG_yC
Cuts at: 0 1759 1884
Size: 1759 125
BsaWI w'CCGG_w
Cuts at: 0 881 1884
Size: 881 1003
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1387 1884
Size: 1387 497
BseYI C'CCAG_C
Cuts at: 0 9 318 359 497 712 1728 1884
Size: 9 309 41 138 215 1016 156
Fragments arranged by size:
1016 309 215 156 138 41 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 526 589 1884
Size: 109 417 63 1295
Fragments arranged by size:
1295 417 109 63
BsiHKAI G_wGCw'C
Cuts at: 0 56 110 371 422 1738 1884
Size: 56 54 261 51 1316 146
Fragments arranged by size:
1316 261 146 56 54 51
Bsp1286I G_dGCh'C
Cuts at: 0 56 110 150 215 325 371 422 661
Size: 56 54 40 65 110 46 51 239
Cuts at: 661 704 906 982 1020 1219 1492 1504 1714
Size: 43 202 76 38 199 273 12 210
Cuts at: 1714 1738 1884
Size: 24 146
Fragments arranged by size:
273 239 210 202 199 146 110 76
65 56 54 51 46 43 40 38
24 12
BspEI T'CCGG_A
Cuts at: 0 881 1884
Size: 881 1003
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1033 1043 1560 1884
Size: 1033 10 517 324
Fragments arranged by size:
1033 517 324 10
BsrDI GCAATG_nn'
Cuts at: 0 391 1884
Size: 391 1493
BssSI C'ACGA_G
Cuts at: 0 103 1884
Size: 103 1781
BstEII G'GTnAC_C
Cuts at: 0 670 1884
Size: 670 1214
BstYI r'GATC_y
Cuts at: 0 1187 1207 1308 1377 1884
Size: 1187 20 101 69 507
Fragments arranged by size:
1187 507 101 69 20
Bsu36I CC'TnA_GG
Cuts at: 0 1836 1884
Size: 1836 48
BtsI GCAGTG_nn'
Cuts at: 0 1740 1884
Size: 1740 144
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 420 455 1884
Size: 420 35 1429
Fragments arranged by size:
1429 420 35
DraIII CAC_nnn'GTG
Cuts at: 0 30 1884
Size: 30 1854
EaeI y'GGCC_r
Cuts at: 0 78 113 947 1461 1668 1770 1884
Size: 78 35 834 514 207 102 114
Fragments arranged by size:
834 514 207 114 102 78 35
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 1884
Size: 19 1865
EcoICRI GAG'CTC
Cuts at: 0 54 108 420 1736 1884
Size: 54 54 312 1316 148
Fragments arranged by size:
1316 312 148 54 54
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 154 1267 1588 1884
Size: 40 114 1113 321 296
Fragments arranged by size:
1113 321 296 114 40
EcoNI CCTnn'n_nnAGG
Cuts at: 0 115 1884
Size: 115 1769
EcoO109I rG'GnC_Cy
Cuts at: 0 211 212 322 1691 1802 1825 1840 1884
Size: 211 1 110 1369 111 23 15 44
Fragments arranged by size:
1369 211 111 110 44 23 15 1
HaeII r_GCGC'y
Cuts at: 0 1751 1762 1884
Size: 1751 11 122
Fragments arranged by size:
1751 122 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1507 1539 1559 1591 1599 1631 1683 1715
Size: 1507 32 20 32 8 32 52 32
Cuts at: 1715 1842 1874 1884
Size: 127 32 10
Fragments arranged by size:
1507 127 52 32 32 32 32 32
20 10 8
HincII GTy'rAC
Cuts at: 0 839 1884
Size: 839 1045
KasI G'GCGC_C
Cuts at: 0 1758 1884
Size: 1758 126
MfeI C'AATT_G
Cuts at: 0 780 1884
Size: 780 1104
MscI TGG'CCA
Cuts at: 0 115 949 1463 1670 1884
Size: 115 834 514 207 214
Fragments arranged by size:
834 514 214 207 115
MslI CAynn'nnrTG
Cuts at: 0 31 374 565 772 975 1092 1884
Size: 31 343 191 207 203 117 792
Fragments arranged by size:
792 343 207 203 191 117 31
MspA1I CmG'CkG
Cuts at: 0 143 459 599 1417 1884
Size: 143 316 140 818 467
Fragments arranged by size:
818 467 316 143 140
NarI GG'CG_CC
Cuts at: 0 1759 1884
Size: 1759 125
NspI r_CATG'y
Cuts at: 0 345 1097 1884
Size: 345 752 787
Fragments arranged by size:
787 752 345
PasI CC'CwG_GG
Cuts at: 0 207 1795 1884
Size: 207 1588 89
Fragments arranged by size:
1588 207 89
PciI A'CATG_T
Cuts at: 0 1093 1884
Size: 1093 791
PflMI CCAn_nnn'nTGG
Cuts at: 0 1197 1267 1884
Size: 1197 70 617
Fragments arranged by size:
1197 617 70
PpuMI rG'GwC_Cy
Cuts at: 0 1691 1825 1840 1884
Size: 1691 134 15 44
Fragments arranged by size:
1691 134 44 15
PspOMI G'GGCC_C
Cuts at: 0 211 321 1710 1884
Size: 211 110 1389 174
Fragments arranged by size:
1389 211 174 110
PstI C_TGCA'G
Cuts at: 0 846 1040 1747 1884
Size: 846 194 707 137
Fragments arranged by size:
846 707 194 137
PvuII CAG'CTG
Cuts at: 0 143 599 1417 1884
Size: 143 456 818 467
Fragments arranged by size:
818 467 456 143
SacI G_AGCT'C
Cuts at: 0 56 110 422 1738 1884
Size: 56 54 312 1316 146
Fragments arranged by size:
1316 312 146 56 54
SanDI GG'GwC_CC
Cuts at: 0 1691 1884
Size: 1691 193
SexAI A'CCwGG_T
Cuts at: 0 824 1884
Size: 824 1060
SfcI C'TryA_G
Cuts at: 0 257 842 1036 1063 1743 1884
Size: 257 585 194 27 680 141
Fragments arranged by size:
680 585 257 194 141 27
SfoI GGC'GCC
Cuts at: 0 1760 1884
Size: 1760 124
SmlI C'TyrA_G
Cuts at: 0 1806 1884
Size: 1806 78
StyI C'CwwG_G
Cuts at: 0 116 1578 1665 1884
Size: 116 1462 87 219
Fragments arranged by size:
1462 219 116 87
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1498 1850 1884
Size: 1498 352 34
Fragments arranged by size:
1498 352 34
TatI w'GTAC_w
Cuts at: 0 1286 1884
Size: 1286 598
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 75 107 1456 1488 1541 1573 1884
Size: 75 32 1349 32 53 32 311
Fragments arranged by size:
1349 311 75 53 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 64 333 771 1884
Size: 64 269 438 1113
Fragments arranged by size:
1113 438 269 64
Enzymes that do cut:
AarI AcuI AflIII AhdI AleI AlfI AlwNI ApaI
ApaLI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglI Bme1580I BmrI BpmI Bpu10I BpuEI
BsaI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspEI BspMI BsrDI BssSI BstEII BstYI Bsu36I
BtsI CspCI DraIII EaeI EciI EcoICRI Eco57MI EcoNI
EcoO109I HaeII Hin4I HincII KasI MfeI MscI MslI
MspA1I NarI NspI PasI PciI PflMI PpuMI PspOMI
PstI PvuII SacI SanDI SexAI SfcI SfoI SmlI
StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AloI
ApoI AscI AseI AsiSI AvaI AvrII BcgI BfrBI
BglII BlpI BmgBI BmtI BplI BsaAI BsaXI BsiEI
BsiWI BsmI BsmBI BspHI BsrBI BsrFI BsrGI BssHII
BstAPI BstBI BstXI BstZ17I BtgI BtgZI ClaI DraI
DrdI EagI EarI EcoRI EcoRV FalI FseI FspI
FspAI HindIII HpaI KpnI MluI MmeI NaeI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PfoI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SapI SbfI ScaI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI Tth111I XbaI XhoI XmaI XmnI ZraI