Restriction map for
pF1KB6119
(Linear) (Six-base) MAPSORT of: KIBB6119.seq Check: 104 from: 1 to: 1869
KIBB6119.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AatII G_ACGT'C
Cuts at: 0 818 1869
Size: 818 1051
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 1869
Size: 53 1816
AflIII A'CryG_T
Cuts at: 0 12 1869
Size: 12 1857
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1741 1773 1869
Size: 1741 32 96
Fragments arranged by size:
1741 96 32
ApoI r'AATT_y
Cuts at: 0 75 134 199 680 784 1869
Size: 75 59 65 481 104 1085
Fragments arranged by size:
1085 481 104 75 65 59
BamHI G'GATC_C
Cuts at: 0 585 1869
Size: 585 1284
BanI G'GyrC_C
Cuts at: 0 1621 1730 1869
Size: 1621 109 139
Fragments arranged by size:
1621 139 109
BanII G_rGCy'C
Cuts at: 0 41 745 1759 1869
Size: 41 704 1014 110
Fragments arranged by size:
1014 704 110 41
BbeI G_GCGC'C
Cuts at: 0 1734 1869
Size: 1734 135
BbsI GAAGACnn'nnnn_
Cuts at: 0 215 304 1869
Size: 215 89 1565
Fragments arranged by size:
1565 215 89
BciVI GTATCCnnnnn_n'
Cuts at: 0 39 1839 1869
Size: 39 1800 30
Fragments arranged by size:
1800 39 30
BclI T'GATC_A
Cuts at: 0 1794 1869
Size: 1794 75
BglII A'GATC_T
Cuts at: 0 513 1869
Size: 513 1356
BlpI GC'TnA_GC
Cuts at: 0 1772 1869
Size: 1772 97
Bme1580I G_kGCm'C
Cuts at: 0 1626 1869
Size: 1626 243
BmrI ACTGGGnnnn_n'
Cuts at: 0 1761 1869
Size: 1761 108
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 428 1355 1869
Size: 428 927 514
Fragments arranged by size:
927 514 428
Bpu10I CC'TnA_GC
Cuts at: 0 647 1869
Size: 647 1222
BsaI GGTCTCn'nnnn_
Cuts at: 0 604 1869
Size: 604 1265
BsaAI yAC'GTr
Cuts at: 0 1651 1869
Size: 1651 218
BsaBI GATnn'nnATC
Cuts at: 0 986 1869
Size: 986 883
BsaHI Gr'CG_yC
Cuts at: 0 815 1731 1869
Size: 815 916 138
Fragments arranged by size:
916 815 138
BsaWI w'CCGG_w
Cuts at: 0 834 1869
Size: 834 1035
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1741 1771 1869
Size: 1741 30 98
Fragments arranged by size:
1741 98 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1517 1535 1592 1631 1869
Size: 1517 18 57 39 238
Fragments arranged by size:
1517 238 57 39 18
Bsp1286I G_dGCh'C
Cuts at: 0 41 745 1626 1759 1869
Size: 41 704 881 133 110
Fragments arranged by size:
881 704 133 110 41
BspHI T'CATG_A
Cuts at: 0 293 675 1869
Size: 293 382 1194
Fragments arranged by size:
1194 382 293
BsrBI CCG'CTC
Cuts at: 0 1782 1869
Size: 1782 87
BsrDI GCAATG_nn'
Cuts at: 0 85 1766 1869
Size: 85 1681 103
Fragments arranged by size:
1681 103 85
BsrFI r'CCGG_y
Cuts at: 0 1561 1869
Size: 1561 308
BsrGI T'GTAC_A
Cuts at: 0 631 1869
Size: 631 1238
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1513 1869
Size: 1513 356
BstYI r'GATC_y
Cuts at: 0 503 513 567 585 981 1446 1869
Size: 503 10 54 18 396 465 423
Fragments arranged by size:
503 465 423 396 54 18 10
Bsu36I CC'TnA_GG
Cuts at: 0 992 1869
Size: 992 877
BtgI C'CryG_G
Cuts at: 0 41 1551 1641 1668 1869
Size: 41 1510 90 27 201
Fragments arranged by size:
1510 201 90 41 27
DraI TTT'AAA
Cuts at: 0 194 240 1869
Size: 194 46 1629
Fragments arranged by size:
1629 194 46
DraIII CAC_nnn'GTG
Cuts at: 0 163 1869
Size: 163 1706
EaeI y'GGCC_r
Cuts at: 0 1665 1869
Size: 1665 204
EarI CTCTTCn'nnn_
Cuts at: 0 29 1046 1869
Size: 29 1017 823
Fragments arranged by size:
1017 823 29
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 53 428 1355 1869
Size: 53 375 927 514
Fragments arranged by size:
927 514 375 53
EcoO109I rG'GnC_Cy
Cuts at: 0 1582 1869
Size: 1582 287
EcoRI G'AATT_C
Cuts at: 0 199 1869
Size: 199 1670
EcoRV GAT'ATC
Cuts at: 0 1366 1869
Size: 1366 503
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 396 1869
Size: 396 1473
HaeII r_GCGC'y
Cuts at: 0 1734 1869
Size: 1734 135
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1566 1598 1728 1760 1869
Size: 1566 32 130 32 109
Fragments arranged by size:
1566 130 109 32 32
HindIII A'AGCT_T
Cuts at: 0 188 1869
Size: 188 1681
KasI G'GCGC_C
Cuts at: 0 1730 1869
Size: 1730 139
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 930 1348 1458 1869
Size: 930 418 110 411
Fragments arranged by size:
930 418 411 110
MslI CAynn'nnrTG
Cuts at: 0 17 701 1133 1869
Size: 17 684 432 736
Fragments arranged by size:
736 684 432 17
MspA1I CmG'CkG
Cuts at: 0 895 1553 1643 1670 1869
Size: 895 658 90 27 199
Fragments arranged by size:
895 658 199 90 27
NarI GG'CG_CC
Cuts at: 0 1731 1869
Size: 1731 138
NcoI C'CATG_G
Cuts at: 0 41 1869
Size: 41 1828
NdeI CA'TA_TG
Cuts at: 0 1313 1869
Size: 1313 556
NspI r_CATG'y
Cuts at: 0 16 1869
Size: 16 1853
PciI A'CATG_T
Cuts at: 0 12 1869
Size: 12 1857
PflMI CCAn_nnn'nTGG
Cuts at: 0 595 1456 1869
Size: 595 861 413
Fragments arranged by size:
861 595 413
PfoI T'CCnGG_A
Cuts at: 0 1584 1869
Size: 1584 285
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1741 1773 1869
Size: 1741 32 96
Fragments arranged by size:
1741 96 32
PpuMI rG'GwC_Cy
Cuts at: 0 1582 1869
Size: 1582 287
PstI C_TGCA'G
Cuts at: 0 60 1869
Size: 60 1809
PvuII CAG'CTG
Cuts at: 0 895 1869
Size: 895 974
SacII CC_GC'GG
Cuts at: 0 1554 1644 1671 1869
Size: 1554 90 27 198
Fragments arranged by size:
1554 198 90 27
SapI GCTCTTCn'nnn_
Cuts at: 0 29 1869
Size: 29 1840
SfcI C'TryA_G
Cuts at: 0 5 56 974 1869
Size: 5 51 918 895
Fragments arranged by size:
918 895 51 5
SfoI GGC'GCC
Cuts at: 0 1732 1869
Size: 1732 137
SspI AAT'ATT
Cuts at: 0 1200 1869
Size: 1200 669
StyI C'CwwG_G
Cuts at: 0 41 764 1785 1869
Size: 41 723 1021 84
Fragments arranged by size:
1021 723 84 41
TatI w'GTAC_w
Cuts at: 0 631 1869
Size: 631 1238
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1429 1869
Size: 1429 440
XmnI GAAnn'nnTTC
Cuts at: 0 77 533 773 1469 1869
Size: 77 456 240 696 400
Fragments arranged by size:
696 456 400 240 77
ZraI GAC'GTC
Cuts at: 0 816 1869
Size: 816 1053
Enzymes that do cut:
AatII AcuI AflIII AloI ApoI BamHI BanI BanII
BbeI BbsI BciVI BclI BglII BlpI Bme1580I BmrI
BpmI Bpu10I BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI Bsp1286I BspHI BsrBI BsrDI BsrFI BsrGI BstAPI
BstYI Bsu36I BtgI DraI DraIII EaeI EarI Eco57MI
EcoO109I EcoRI EcoRV FalI HaeII Hin4I HindIII KasI
MmeI MslI MspA1I NarI NcoI NdeI NspI PciI
PflMI PfoI PpiI PpuMI PstI PvuII SacII SapI
SfcI SfoI SspI StyI TatI XcmI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AlwNI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI
BmtI BplI BpuEI BseYI BsgI BsiEI BsiHKAI BsiWI
BsmI BsmBI BspEI BspMI BssHII BssSI BstBI BstEII
BstXI BstZ17I BtgZI BtsI ClaI CspCI DrdI EagI
EciI EcoICRI EcoNI FseI FspI FspAI HincII HpaI
KpnI MfeI MluI MscI NaeI NgoMIV NheI NotI
NruI NsiI PacI PasI PmeI PmlI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SalI SanDI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI StuI SwaI TaqII TstI
Tth111I XbaI XhoI XmaI