Restriction map for
pF1KB6123
(Linear) (Six-base) MAPSORT of: KIBB6123.seq Check: 710 from: 1 to: 1758
KIBB6123.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AccI GT'mk_AC
Cuts at: 0 101 786 1758
Size: 101 685 972
Fragments arranged by size:
972 685 101
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 151 374 497 665 1758
Size: 82 69 223 123 168 1093
Fragments arranged by size:
1093 223 168 123 82 69
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1097 1101 1758
Size: 1097 4 657
Fragments arranged by size:
1097 657 4
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 103 135 1758
Size: 103 32 1623
Fragments arranged by size:
1623 103 32
ApaLI G'TGCA_C
Cuts at: 0 16 531 1758
Size: 16 515 1227
Fragments arranged by size:
1227 515 16
ApoI r'AATT_y
Cuts at: 0 380 696 723 880 1758
Size: 380 316 27 157 878
Fragments arranged by size:
878 380 316 157 27
BanI G'GyrC_C
Cuts at: 0 79 1758
Size: 79 1679
BanII G_rGCy'C
Cuts at: 0 38 311 1758
Size: 38 273 1447
Fragments arranged by size:
1447 273 38
BbeI G_GCGC'C
Cuts at: 0 83 1758
Size: 83 1675
BbsI GAAGACnn'nnnn_
Cuts at: 0 1486 1758
Size: 1486 272
BbvCI CC'TCA_GC
Cuts at: 0 1421 1758
Size: 1421 337
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 711 745 1758
Size: 711 34 1013
Fragments arranged by size:
1013 711 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1369 1758
Size: 1369 389
BglI GCCn_nnn'nGGC
Cuts at: 0 988 1004 1758
Size: 988 16 754
Fragments arranged by size:
988 754 16
BglII A'GATC_T
Cuts at: 0 810 1457 1758
Size: 810 647 301
Fragments arranged by size:
810 647 301
Bme1580I G_kGCm'C
Cuts at: 0 20 535 1675 1758
Size: 20 515 1140 83
Fragments arranged by size:
1140 515 83 20
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 380 1758
Size: 380 1378
Bpu10I CC'TnA_GC
Cuts at: 0 1421 1758
Size: 1421 337
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 1577 1758
Size: 196 1381 181
Fragments arranged by size:
1381 196 181
BsaI GGTCTCn'nnnn_
Cuts at: 0 1684 1758
Size: 1684 74
BsaBI GATnn'nnATC
Cuts at: 0 191 965 1758
Size: 191 774 793
Fragments arranged by size:
793 774 191
BsaHI Gr'CG_yC
Cuts at: 0 80 1758
Size: 80 1678
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 353 383 1758
Size: 353 30 1375
Fragments arranged by size:
1375 353 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 120 530 1758
Size: 120 410 1228
Fragments arranged by size:
1228 410 120
BsiHKAI G_wGCw'C
Cuts at: 0 20 535 1753 1758
Size: 20 515 1218 5
Fragments arranged by size:
1218 515 20 5
BsmI GAATG_Cn'
Cuts at: 0 71 723 1350 1758
Size: 71 652 627 408
Fragments arranged by size:
652 627 408 71
BsmBI CGTCTCn'nnnn_
Cuts at: 0 320 1758
Size: 320 1438
Bsp1286I G_dGCh'C
Cuts at: 0 20 38 311 535 1675 1753 1758
Size: 20 18 273 224 1140 78 5
Fragments arranged by size:
1140 273 224 78 20 18 5
BspHI T'CATG_A
Cuts at: 0 1028 1758
Size: 1028 730
BsrFI r'CCGG_y
Cuts at: 0 124 1758
Size: 124 1634
BssSI C'ACGA_G
Cuts at: 0 1097 1758
Size: 1097 661
BstYI r'GATC_y
Cuts at: 0 180 810 1266 1457 1758
Size: 180 630 456 191 301
Fragments arranged by size:
630 456 301 191 180
Bsu36I CC'TnA_GG
Cuts at: 0 971 1758
Size: 971 787
BtgI C'CryG_G
Cuts at: 0 1012 1667 1758
Size: 1012 655 91
Fragments arranged by size:
1012 655 91
BtsI GCAGTG_nn'
Cuts at: 0 629 1230 1584 1758
Size: 629 601 354 174
Fragments arranged by size:
629 601 354 174
EaeI y'GGCC_r
Cuts at: 0 628 1758
Size: 628 1130
EarI CTCTTCn'nnn_
Cuts at: 0 581 1703 1758
Size: 581 1122 55
Fragments arranged by size:
1122 581 55
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 650 1006 1758
Size: 650 356 752
Fragments arranged by size:
752 650 356
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 151 374 380 497 665 1758
Size: 82 69 223 6 117 168 1093
Fragments arranged by size:
1093 223 168 117 82 69 6
EcoO109I rG'GnC_Cy
Cuts at: 0 1104 1758
Size: 1104 654
EcoRI G'AATT_C
Cuts at: 0 723 1758
Size: 723 1035
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 701 1758
Size: 701 1057
HaeII r_GCGC'y
Cuts at: 0 83 1758
Size: 83 1675
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 353 385 598 630 1047 1079 1251 1268
Size: 353 32 213 32 417 32 172 17
Cuts at: 1268 1283 1300 1713 1745 1758
Size: 15 17 413 32 13
Fragments arranged by size:
417 413 353 213 172 32 32 32
32 17 17 15 13
HincII GTy'rAC
Cuts at: 0 787 1758
Size: 787 971
HindIII A'AGCT_T
Cuts at: 0 683 1758
Size: 683 1075
KasI G'GCGC_C
Cuts at: 0 79 1758
Size: 79 1679
MfeI C'AATT_G
Cuts at: 0 290 1758
Size: 290 1468
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1033 1168 1758
Size: 1033 135 590
Fragments arranged by size:
1033 590 135
MscI TGG'CCA
Cuts at: 0 630 1758
Size: 630 1128
MspA1I CmG'CkG
Cuts at: 0 95 1758
Size: 95 1663
NaeI GCC'GGC
Cuts at: 0 126 1758
Size: 126 1632
NarI GG'CG_CC
Cuts at: 0 80 1758
Size: 80 1678
NcoI C'CATG_G
Cuts at: 0 1012 1667 1758
Size: 1012 655 91
Fragments arranged by size:
1012 655 91
NgoMIV G'CCGG_C
Cuts at: 0 124 1758
Size: 124 1634
NspI r_CATG'y
Cuts at: 0 1078 1758
Size: 1078 680
PfoI T'CCnGG_A
Cuts at: 0 107 1758
Size: 107 1651
PpuMI rG'GwC_Cy
Cuts at: 0 1104 1758
Size: 1104 654
PsiI TTA'TAA
Cuts at: 0 449 1758
Size: 449 1309
PstI C_TGCA'G
Cuts at: 0 624 1554 1758
Size: 624 930 204
Fragments arranged by size:
930 624 204
SalI G'TCGA_C
Cuts at: 0 785 1758
Size: 785 973
SapI GCTCTTCn'nnn_
Cuts at: 0 581 1758
Size: 581 1177
SfcI C'TryA_G
Cuts at: 0 620 1550 1758
Size: 620 930 208
Fragments arranged by size:
930 620 208
SfoI GGC'GCC
Cuts at: 0 81 1758
Size: 81 1677
SmlI C'TyrA_G
Cuts at: 0 175 1592 1758
Size: 175 1417 166
Fragments arranged by size:
1417 175 166
SphI G_CATG'C
Cuts at: 0 1078 1758
Size: 1078 680
StyI C'CwwG_G
Cuts at: 0 955 1012 1667 1758
Size: 955 57 655 91
Fragments arranged by size:
955 655 91 57
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 678 1758
Size: 678 1080
Tth111I GACn'n_nGTC
Cuts at: 0 752 1490 1758
Size: 752 738 268
Fragments arranged by size:
752 738 268
Enzymes that do cut:
AccI AcuI AlfI AloI ApaLI ApoI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BglI BglII Bme1580I
BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaXI BseRI
BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrFI BssSI BstYI
Bsu36I BtgI BtsI EaeI EarI EciI Eco57MI EcoO109I
EcoRI FalI HaeII Hin4I HincII HindIII KasI MfeI
MmeI MscI MspA1I NaeI NarI NcoI NgoMIV NspI
PfoI PpuMI PsiI PstI SalI SapI SfcI SfoI
SmlI SphI StyI TaqII Tth111I
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlwNI ApaI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BclI BfrBI BlpI BmgBI BmrI
BmtI BplI BsaAI BsaWI BseYI BsgI BsiEI BsiWI
BspEI BspMI BsrBI BsrDI BsrGI BssHII BstAPI BstBI
BstEII BstXI BstZ17I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EcoICRI EcoNI EcoRV FseI FspI FspAI
HpaI KpnI MluI MslI NdeI NheI NotI NruI
NsiI PacI PasI PciI PflMI PmeI PmlI PpiI
PshAI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SacII SanDI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI StuI SwaI TatI
TstI XbaI XcmI XhoI XmaI XmnI ZraI