Restriction map for
pF1KB6124
(Linear) (Six-base) MAPSORT of: KIBB6124.seq Check: 8532 from: 1 to: 1878
KIBB6124.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AccI GT'mk_AC
Cuts at: 0 1058 1878
Size: 1058 820
Acc65I G'GTAC_C
Cuts at: 0 85 294 1878
Size: 85 209 1584
Fragments arranged by size:
1584 209 85
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 752 1878
Size: 752 1126
AlwNI CAG_nnn'CTG
Cuts at: 0 523 1831 1878
Size: 523 1308 47
Fragments arranged by size:
1308 523 47
ApaI G_GGCC'C
Cuts at: 0 58 1878
Size: 58 1820
ApoI r'AATT_y
Cuts at: 0 1213 1878
Size: 1213 665
AvaI C'yCGr_G
Cuts at: 0 51 644 1463 1878
Size: 51 593 819 415
Fragments arranged by size:
819 593 415 51
BanI G'GyrC_C
Cuts at: 0 85 294 379 1878
Size: 85 209 85 1499
Fragments arranged by size:
1499 209 85 85
BanII G_rGCy'C
Cuts at: 0 58 1878
Size: 58 1820
BbsI GAAGACnn'nnnn_
Cuts at: 0 519 788 905 1878
Size: 519 269 117 973
Fragments arranged by size:
973 519 269 117
BbvCI CC'TCA_GC
Cuts at: 0 931 1499 1878
Size: 931 568 379
Fragments arranged by size:
931 568 379
Bme1580I G_kGCm'C
Cuts at: 0 58 1878
Size: 58 1820
BmrI ACTGGGnnnn_n'
Cuts at: 0 1772 1846 1878
Size: 1772 74 32
Fragments arranged by size:
1772 74 32
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 639 1598 1878
Size: 639 959 280
Fragments arranged by size:
959 639 280
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 743 1427 1539 1586 1619 1878
Size: 743 684 112 47 33 259
Fragments arranged by size:
743 684 259 112 47 33
Bpu10I CC'TnA_GC
Cuts at: 0 343 931 1499 1673 1878
Size: 343 588 568 174 205
Fragments arranged by size:
588 568 343 205 174
BsaHI Gr'CG_yC
Cuts at: 0 249 1878
Size: 249 1629
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 615 618 1878
Size: 615 3 1260
Fragments arranged by size:
1260 615 3
BseYI C'CCAG_C
Cuts at: 0 1468 1878
Size: 1468 410
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 822 1878
Size: 822 1056
BsiHKAI G_wGCw'C
Cuts at: 0 729 1878
Size: 729 1149
BsmI GAATG_Cn'
Cuts at: 0 960 1878
Size: 960 918
Bsp1286I G_dGCh'C
Cuts at: 0 58 729 1878
Size: 58 671 1149
Fragments arranged by size:
1149 671 58
BspHI T'CATG_A
Cuts at: 0 1268 1808 1878
Size: 1268 540 70
Fragments arranged by size:
1268 540 70
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 74 593 811 1878
Size: 74 519 218 1067
Fragments arranged by size:
1067 519 218 74
BsrBI CCG'CTC
Cuts at: 0 651 1878
Size: 651 1227
BsrDI GCAATG_nn'
Cuts at: 0 317 1802 1878
Size: 317 1485 76
Fragments arranged by size:
1485 317 76
BssSI C'ACGA_G
Cuts at: 0 1572 1878
Size: 1572 306
BstXI CCAn_nnnn'nTGG
Cuts at: 0 96 885 1335 1878
Size: 96 789 450 543
Fragments arranged by size:
789 543 450 96
BstYI r'GATC_y
Cuts at: 0 533 1878
Size: 533 1345
BtgI C'CryG_G
Cuts at: 0 1867 1878
Size: 1867 11
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1520 1878
Size: 1520 358
DraI TTT'AAA
Cuts at: 0 1218 1622 1878
Size: 1218 404 256
Fragments arranged by size:
1218 404 256
DraIII CAC_nnn'GTG
Cuts at: 0 1115 1878
Size: 1115 763
EaeI y'GGCC_r
Cuts at: 0 871 1870 1878
Size: 871 999 8
Fragments arranged by size:
999 871 8
EarI CTCTTCn'nnn_
Cuts at: 0 1602 1878
Size: 1602 276
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 743 752 1427 1539 1586 1619 1878
Size: 743 9 675 112 47 33 259
Fragments arranged by size:
743 675 259 112 47 33 9
EcoO109I rG'GnC_Cy
Cuts at: 0 55 1347 1427 1878
Size: 55 1292 80 451
Fragments arranged by size:
1292 451 80 55
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 525 1878
Size: 525 1353
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 31 63 781 813 1750 1782 1878
Size: 31 32 718 32 937 32 96
Fragments arranged by size:
937 718 96 32 32 32 31
HincII GTy'rAC
Cuts at: 0 1851 1878
Size: 1851 27
HindIII A'AGCT_T
Cuts at: 0 831 1878
Size: 831 1047
KpnI G_GTAC'C
Cuts at: 0 89 298 1878
Size: 89 209 1580
Fragments arranged by size:
1580 209 89
MfeI C'AATT_G
Cuts at: 0 947 1878
Size: 947 931
MscI TGG'CCA
Cuts at: 0 873 1878
Size: 873 1005
MslI CAynn'nnrTG
Cuts at: 0 883 965 1136 1878
Size: 883 82 171 742
Fragments arranged by size:
883 742 171 82
MspA1I CmG'CkG
Cuts at: 0 935 1875 1878
Size: 935 940 3
Fragments arranged by size:
940 935 3
NcoI C'CATG_G
Cuts at: 0 1867 1878
Size: 1867 11
NspI r_CATG'y
Cuts at: 0 305 1398 1878
Size: 305 1093 480
Fragments arranged by size:
1093 480 305
PasI CC'CwG_GG
Cuts at: 0 700 1878
Size: 700 1178
PflMI CCAn_nnn'nTGG
Cuts at: 0 15 1386 1878
Size: 15 1371 492
Fragments arranged by size:
1371 492 15
PfoI T'CCnGG_A
Cuts at: 0 316 1334 1602 1878
Size: 316 1018 268 276
Fragments arranged by size:
1018 316 276 268
PpuMI rG'GwC_Cy
Cuts at: 0 1347 1427 1878
Size: 1347 80 451
Fragments arranged by size:
1347 451 80
PspOMI G'GGCC_C
Cuts at: 0 54 1878
Size: 54 1824
PvuII CAG'CTG
Cuts at: 0 935 1878
Size: 935 943
SanDI GG'GwC_CC
Cuts at: 0 1347 1878
Size: 1347 531
SfcI C'TryA_G
Cuts at: 0 543 744 1878
Size: 543 201 1134
Fragments arranged by size:
1134 543 201
SmaI CCC'GGG
Cuts at: 0 53 646 1465 1878
Size: 53 593 819 413
Fragments arranged by size:
819 593 413 53
SphI G_CATG'C
Cuts at: 0 1398 1878
Size: 1398 480
SspI AAT'ATT
Cuts at: 0 1126 1878
Size: 1126 752
StuI AGG'CCT
Cuts at: 0 765 1878
Size: 765 1113
StyI C'CwwG_G
Cuts at: 0 198 1276 1283 1761 1867 1878
Size: 198 1078 7 478 106 11
Fragments arranged by size:
1078 478 198 106 11 7
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 509 891 1828 1878
Size: 509 382 937 50
Fragments arranged by size:
937 509 382 50
XmaI C'CCGG_G
Cuts at: 0 51 644 1463 1878
Size: 51 593 819 415
Fragments arranged by size:
819 593 415 51
XmnI GAAnn'nnTTC
Cuts at: 0 1127 1878
Size: 1127 751
Enzymes that do cut:
AccI Acc65I AcuI AlwNI ApaI ApoI AvaI BanI
BanII BbsI BbvCI Bme1580I BmrI BplI BpmI Bpu10I
BsaHI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI
BspMI BsrBI BsrDI BssSI BstXI BstYI BtgI BtgZI
DraI DraIII EaeI EarI Eco57MI EcoO109I FalI Hin4I
HincII HindIII KpnI MfeI MscI MslI MspA1I NcoI
NspI PasI PflMI PfoI PpuMI PspOMI PvuII SanDI
SfcI SmaI SphI SspI StuI StyI TaqII XmaI
XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BcgI BciVI BclI BfrBI BglI
BglII BlpI BmgBI BmtI BpuEI BsaI BsaAI BsaBI
BsaWI BsaXI BsiEI BsiWI BsmBI BspEI BsrFI BsrGI
BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtsI ClaI
CspCI DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV
FseI FspI FspAI HaeII HpaI KasI MluI MmeI
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SrfI SwaI TatI TstI Tth111I XbaI
XcmI XhoI ZraI