Restriction map for
pF1KB6131
(Linear) (Six-base) MAPSORT of: KIBB6131.seq Check: 8701 from: 1 to: 1701
KIBB6131.orf
With 182 enzymes: SgfI *
June 2, 2009 16:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 555 879 1379 1403 1701
Size: 555 324 500 24 298
Fragments arranged by size:
555 500 324 298 24
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 520 653 1597 1701
Size: 520 133 944 104
Fragments arranged by size:
944 520 133 104
AflIII A'CryG_T
Cuts at: 0 774 1157 1701
Size: 774 383 544
Fragments arranged by size:
774 544 383
AgeI A'CCGG_T
Cuts at: 0 1328 1701
Size: 1328 373
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 826 1701
Size: 826 875
AlwNI CAG_nnn'CTG
Cuts at: 0 183 210 468 1689 1701
Size: 183 27 258 1221 12
Fragments arranged by size:
1221 258 183 27 12
ApaI G_GGCC'C
Cuts at: 0 106 1701
Size: 106 1595
AvaI C'yCGr_G
Cuts at: 0 52 107 224 310 588 1701
Size: 52 55 117 86 278 1113
Fragments arranged by size:
1113 278 117 86 55 52
BanI G'GyrC_C
Cuts at: 0 891 924 1295 1336 1556 1701
Size: 891 33 371 41 220 145
Fragments arranged by size:
891 371 220 145 41 33
BanII G_rGCy'C
Cuts at: 0 10 106 311 413 476 1072 1701
Size: 10 96 205 102 63 596 629
Fragments arranged by size:
629 596 205 102 96 63 10
BbsI GAAGACnn'nnnn_
Cuts at: 0 350 1701
Size: 350 1351
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 715 749 1701
Size: 715 34 952
Fragments arranged by size:
952 715 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1382 1701
Size: 1382 319
BglI GCCn_nnn'nGGC
Cuts at: 0 162 1701
Size: 162 1539
BglII A'GATC_T
Cuts at: 0 744 1701
Size: 744 957
BlpI GC'TnA_GC
Cuts at: 0 499 1701
Size: 499 1202
Bme1580I G_kGCm'C
Cuts at: 0 106 815 1298 1701
Size: 106 709 483 403
Fragments arranged by size:
709 483 403 106
BmrI ACTGGGnnnn_n'
Cuts at: 0 505 1701
Size: 505 1196
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 205 220 562 781 829 996 1209
Size: 35 170 15 342 219 48 167 213
Cuts at: 1209 1396 1701
Size: 187 305
Fragments arranged by size:
342 305 219 213 187 170 167 48
35 15
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 1701
Size: 316 1385
BsaI GGTCTCn'nnnn_
Cuts at: 0 309 714 825 1701
Size: 309 405 111 876
Fragments arranged by size:
876 405 309 111
BsaAI yAC'GTr
Cuts at: 0 1158 1701
Size: 1158 543
BsaHI Gr'CG_yC
Cuts at: 0 66 1701
Size: 66 1635
BsaWI w'CCGG_w
Cuts at: 0 1106 1328 1701
Size: 1106 222 373
Fragments arranged by size:
1106 373 222
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1015 1045 1118 1148 1360 1390 1701
Size: 1015 30 73 30 212 30 311
Fragments arranged by size:
1015 311 212 73 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 18 775 895 1633 1701
Size: 18 757 120 738 68
Fragments arranged by size:
757 738 120 68 18
BseYI C'CCAG_C
Cuts at: 0 46 239 443 737 942 1034 1062 1701
Size: 46 193 204 294 205 92 28 639
Fragments arranged by size:
639 294 205 204 193 92 46 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1035 1156 1645 1701
Size: 1035 121 489 56
Fragments arranged by size:
1035 489 121 56
BsiHKAI G_wGCw'C
Cuts at: 0 1252 1701
Size: 1252 449
BsmBI CGTCTCn'nnnn_
Cuts at: 0 73 1701
Size: 73 1628
Bsp1286I G_dGCh'C
Cuts at: 0 10 106 311 413 476 815 1072 1252
Size: 10 96 205 102 63 339 257 180
Cuts at: 1252 1298 1701
Size: 46 403
Fragments arranged by size:
403 339 257 205 180 102 96 63
46 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 555 879 1379 1403 1701
Size: 555 324 500 24 298
Fragments arranged by size:
555 500 324 298 24
BsrBI CCG'CTC
Cuts at: 0 266 1701
Size: 266 1435
BsrFI r'CCGG_y
Cuts at: 0 402 1328 1382 1416 1604 1701
Size: 402 926 54 34 188 97
Fragments arranged by size:
926 402 188 97 54 34
BssSI C'ACGA_G
Cuts at: 0 1243 1309 1651 1701
Size: 1243 66 342 50
Fragments arranged by size:
1243 342 66 50
BstEII G'GTnAC_C
Cuts at: 0 567 1701
Size: 567 1134
BstXI CCAn_nnnn'nTGG
Cuts at: 0 456 1701
Size: 456 1245
BstYI r'GATC_y
Cuts at: 0 230 654 744 792 1701
Size: 230 424 90 48 909
Fragments arranged by size:
909 424 230 90 48
BtgI C'CryG_G
Cuts at: 0 284 1701
Size: 284 1417
BtsI GCAGTG_nn'
Cuts at: 0 331 1701
Size: 331 1370
EaeI y'GGCC_r
Cuts at: 0 212 281 1701
Size: 212 69 1420
Fragments arranged by size:
1420 212 69
EarI CTCTTCn'nnn_
Cuts at: 0 76 536 629 1052 1342 1420 1701
Size: 76 460 93 423 290 78 281
Fragments arranged by size:
460 423 290 281 93 78 76
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 64 1345 1401 1636 1701
Size: 64 1281 56 235 65
Fragments arranged by size:
1281 235 65 64 56
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 205 220 520 562 653 781 829
Size: 35 170 15 300 42 91 128 48
Cuts at: 829 996 1209 1396 1597 1701
Size: 167 213 187 201 104
Fragments arranged by size:
300 213 201 187 170 167 128 104
91 48 42 35 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 535 1701
Size: 535 1166
EcoO109I rG'GnC_Cy
Cuts at: 0 103 114 132 507 1284 1462 1701
Size: 103 11 18 375 777 178 239
Fragments arranged by size:
777 375 239 178 103 18 11
HaeII r_GCGC'y
Cuts at: 0 126 1659 1674 1701
Size: 126 1533 15 27
Fragments arranged by size:
1533 126 27 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 628 660 671 703 1015 1047 1701
Size: 628 32 11 32 312 32 654
Fragments arranged by size:
654 628 312 32 32 32 11
HincII GTy'rAC
Cuts at: 0 1197 1701
Size: 1197 504
MluI A'CGCG_T
Cuts at: 0 774 1701
Size: 774 927
MscI TGG'CCA
Cuts at: 0 214 1701
Size: 214 1487
MspA1I CmG'CkG
Cuts at: 0 286 399 503 946 1187 1476 1536 1701
Size: 286 113 104 443 241 289 60 165
Fragments arranged by size:
443 289 286 241 165 113 104 60
PasI CC'CwG_GG
Cuts at: 0 33 753 1701
Size: 33 720 948
Fragments arranged by size:
948 720 33
PflMI CCAn_nnn'nTGG
Cuts at: 0 1419 1701
Size: 1419 282
PpuMI rG'GwC_Cy
Cuts at: 0 132 507 1284 1462 1701
Size: 132 375 777 178 239
Fragments arranged by size:
777 375 239 178 132
PshAI GACnn'nnGTC
Cuts at: 0 1460 1701
Size: 1460 241
PspOMI G'GGCC_C
Cuts at: 0 102 1701
Size: 102 1599
PstI C_TGCA'G
Cuts at: 0 122 890 1400 1701
Size: 122 768 510 301
Fragments arranged by size:
768 510 301 122
PvuII CAG'CTG
Cuts at: 0 399 946 1701
Size: 399 547 755
Fragments arranged by size:
755 547 399
SacII CC_GC'GG
Cuts at: 0 287 1701
Size: 287 1414
SanDI GG'GwC_CC
Cuts at: 0 507 1462 1701
Size: 507 955 239
Fragments arranged by size:
955 507 239
SapI GCTCTTCn'nnn_
Cuts at: 0 1052 1342 1701
Size: 1052 290 359
Fragments arranged by size:
1052 359 290
SfcI C'TryA_G
Cuts at: 0 118 886 1396 1701
Size: 118 768 510 305
Fragments arranged by size:
768 510 305 118
SgrAI Cr'CCGG_yG
Cuts at: 0 1382 1701
Size: 1382 319
SmaI CCC'GGG
Cuts at: 0 109 226 312 1701
Size: 109 117 86 1389
Fragments arranged by size:
1389 117 109 86
SmlI C'TyrA_G
Cuts at: 0 331 1701
Size: 331 1370
StuI AGG'CCT
Cuts at: 0 907 1528 1701
Size: 907 621 173
Fragments arranged by size:
907 621 173
StyI C'CwwG_G
Cuts at: 0 170 1701
Size: 170 1531
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 231 1701
Size: 231 1470
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1118 1150 1360 1392 1701
Size: 1118 32 210 32 309
Fragments arranged by size:
1118 309 210 32 32
XbaI T'CTAG_A
Cuts at: 0 651 966 1701
Size: 651 315 735
Fragments arranged by size:
735 651 315
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1473 1701
Size: 1473 228
XmaI C'CCGG_G
Cuts at: 0 107 224 310 1701
Size: 107 117 86 1391
Fragments arranged by size:
1391 117 107 86
XmnI GAAnn'nnTTC
Cuts at: 0 1180 1351 1528 1701
Size: 1180 171 177 173
Fragments arranged by size:
1180 177 173 171
Enzymes that do cut:
AarI AcuI AflIII AgeI AlfI AlwNI ApaI AvaI
BanI BanII BbsI BcgI BciVI BglI BglII BlpI
Bme1580I BmrI BpmI BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI
BsrBI BsrFI BssSI BstEII BstXI BstYI BtgI BtsI
EaeI EarI EciI Eco57MI EcoNI EcoO109I HaeII Hin4I
HincII MluI MscI MspA1I PasI PflMI PpuMI PshAI
PspOMI PstI PvuII SacII SanDI SapI SfcI SgrAI
SmaI SmlI StuI StyI TaqII TstI XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AhdI AleI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbvCI BclI BfrBI BmgBI BmtI BplI
Bpu10I BsaBI BsiEI BsiWI BsmI BspEI BspHI BsrDI
BsrGI BssHII BstAPI BstBI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EagI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI HindIII HpaI KasI KpnI
MfeI MmeI MslI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PfoI
PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII
SacI SalI SbfI ScaI SexAI SfiI SfoI SgfI
SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I
XhoI ZraI