Restriction map for
pF1KB6132
(Linear) (Six-base) MAPSORT of: KIBB6132.seq Check: 1341 from: 1 to: 1884
KIBB6132.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 520 1884
Size: 520 1364
AccI GT'mk_AC
Cuts at: 0 901 1884
Size: 901 983
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 901 1884
Size: 901 983
AflIII A'CryG_T
Cuts at: 0 368 425 1884
Size: 368 57 1459
Fragments arranged by size:
1459 368 57
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 98 1884
Size: 98 1786
AlwNI CAG_nnn'CTG
Cuts at: 0 400 573 1884
Size: 400 173 1311
Fragments arranged by size:
1311 400 173
ApaI G_GGCC'C
Cuts at: 0 39 1729 1884
Size: 39 1690 155
Fragments arranged by size:
1690 155 39
ApaLI G'TGCA_C
Cuts at: 0 1156 1884
Size: 1156 728
AvaI C'yCGr_G
Cuts at: 0 213 284 636 730 952 983 1791 1884
Size: 213 71 352 94 222 31 808 93
Fragments arranged by size:
808 352 222 213 94 93 71 31
BanI G'GyrC_C
Cuts at: 0 323 579 1258 1612 1834 1884
Size: 323 256 679 354 222 50
Fragments arranged by size:
679 354 323 256 222 50
BanII G_rGCy'C
Cuts at: 0 8 39 222 256 263 1097 1370 1624
Size: 8 31 183 34 7 834 273 254
Cuts at: 1624 1729 1761 1884
Size: 105 32 123
Fragments arranged by size:
834 273 254 183 123 105 34 32
31 8 7
BbeI G_GCGC'C
Cuts at: 0 327 1884
Size: 327 1557
BbsI GAAGACnn'nnnn_
Cuts at: 0 1593 1692 1884
Size: 1593 99 192
Fragments arranged by size:
1593 192 99
BbvCI CC'TCA_GC
Cuts at: 0 338 404 1300 1884
Size: 338 66 896 584
Fragments arranged by size:
896 584 338 66
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1122 1156 1884
Size: 1122 34 728
Fragments arranged by size:
1122 728 34
BglI GCCn_nnn'nGGC
Cuts at: 0 743 1553 1654 1884
Size: 743 810 101 230
Fragments arranged by size:
810 743 230 101
BlpI GC'TnA_GC
Cuts at: 0 1096 1783 1884
Size: 1096 687 101
Fragments arranged by size:
1096 687 101
Bme1580I G_kGCm'C
Cuts at: 0 39 584 1160 1729 1884
Size: 39 545 576 569 155
Fragments arranged by size:
576 569 545 155 39
BmgBI CAC'GTC
Cuts at: 0 1041 1884
Size: 1041 843
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1532 1884
Size: 1532 352
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 595 991 1081 1111 1407 1473 1661
Size: 247 348 396 90 30 296 66 188
Cuts at: 1661 1884
Size: 223
Fragments arranged by size:
396 348 296 247 223 188 90 66
30
Bpu10I CC'TnA_GC
Cuts at: 0 338 404 1300 1543 1884
Size: 338 66 896 243 341
Fragments arranged by size:
896 341 338 243 66
BsaI GGTCTCn'nnnn_
Cuts at: 0 459 1884
Size: 459 1425
BsaAI yAC'GTr
Cuts at: 0 369 551 798 1266 1884
Size: 369 182 247 468 618
Fragments arranged by size:
618 468 369 247 182
BsaHI Gr'CG_yC
Cuts at: 0 324 695 1884
Size: 324 371 1189
Fragments arranged by size:
1189 371 324
BsaWI w'CCGG_w
Cuts at: 0 1840 1884
Size: 1840 44
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1388 1418 1634 1664 1884
Size: 1388 30 216 30 220
Fragments arranged by size:
1388 220 216 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1675 1859 1884
Size: 1675 184 25
Fragments arranged by size:
1675 184 25
BseYI C'CCAG_C
Cuts at: 0 828 836 889 1884
Size: 828 8 53 995
Fragments arranged by size:
995 828 53 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 141 763 820 1063 1157 1884
Size: 141 622 57 243 94 727
Fragments arranged by size:
727 622 243 141 94 57
BsiEI CG_ry'CG
Cuts at: 0 210 747 1884
Size: 210 537 1137
Fragments arranged by size:
1137 537 210
BsiHKAI G_wGCw'C
Cuts at: 0 222 263 797 1097 1160 1884
Size: 222 41 534 300 63 724
Fragments arranged by size:
724 534 300 222 63 41
BsmBI CGTCTCn'nnnn_
Cuts at: 0 686 1391 1707 1868 1884
Size: 686 705 316 161 16
Fragments arranged by size:
705 686 316 161 16
Bsp1286I G_dGCh'C
Cuts at: 0 8 39 222 256 263 584 797 1097
Size: 8 31 183 34 7 321 213 300
Cuts at: 1097 1160 1370 1624 1729 1761 1884
Size: 63 210 254 105 32 123
Fragments arranged by size:
321 300 254 213 210 183 123 105
63 34 32 31 8 7
BspEI T'CCGG_A
Cuts at: 0 1840 1884
Size: 1840 44
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 520 677 1884
Size: 520 157 1207
Fragments arranged by size:
1207 520 157
BsrBI CCG'CTC
Cuts at: 0 212 995 1884
Size: 212 783 889
Fragments arranged by size:
889 783 212
BsrFI r'CCGG_y
Cuts at: 0 18 1816 1884
Size: 18 1798 68
Fragments arranged by size:
1798 68 18
BssHII G'CGCG_C
Cuts at: 0 28 1884
Size: 28 1856
BstAPI GCAn_nnn'nTGC
Cuts at: 0 400 1164 1884
Size: 400 764 720
Fragments arranged by size:
764 720 400
BstXI CCAn_nnnn'nTGG
Cuts at: 0 836 1059 1206 1884
Size: 836 223 147 678
Fragments arranged by size:
836 678 223 147
BstZ17I GTA'TAC
Cuts at: 0 902 1884
Size: 902 982
BtgI C'CryG_G
Cuts at: 0 40 741 788 1425 1581 1658 1884
Size: 40 701 47 637 156 77 226
Fragments arranged by size:
701 637 226 156 77 47 40
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1226 1565 1884
Size: 1226 339 319
Fragments arranged by size:
1226 339 319
BtsI GCAGTG_nn'
Cuts at: 0 398 410 705 1140 1884
Size: 398 12 295 435 744
Fragments arranged by size:
744 435 398 295 12
EaeI y'GGCC_r
Cuts at: 0 744 1428 1646 1655 1661 1884
Size: 744 684 218 9 6 223
Fragments arranged by size:
744 684 223 218 9 6
EagI C'GGCC_G
Cuts at: 0 744 1884
Size: 744 1140
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 106 901 1751 1884
Size: 106 795 850 133
Fragments arranged by size:
850 795 133 106
EcoICRI GAG'CTC
Cuts at: 0 220 261 1095 1884
Size: 220 41 834 789
Fragments arranged by size:
834 789 220 41
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 247 595 901 991 1081 1111 1407 1473
Size: 247 348 306 90 90 30 296 66
Cuts at: 1473 1661 1884
Size: 188 223
Fragments arranged by size:
348 306 296 247 223 188 90 90
66 30
EcoNI CCTnn'n_nnAGG
Cuts at: 0 730 1884
Size: 730 1154
EcoO109I rG'GnC_Cy
Cuts at: 0 35 36 998 1725 1726 1884
Size: 35 1 962 727 1 158
Fragments arranged by size:
962 727 158 35 1 1
HaeII r_GCGC'y
Cuts at: 0 327 573 1541 1596 1884
Size: 327 246 968 55 288
Fragments arranged by size:
968 327 288 246 55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1388 1420 1543 1575 1634 1666 1884
Size: 1388 32 123 32 59 32 218
Fragments arranged by size:
1388 218 123 59 32 32 32
KasI G'GCGC_C
Cuts at: 0 323 1884
Size: 323 1561
MfeI C'AATT_G
Cuts at: 0 1459 1884
Size: 1459 425
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1598 1778 1884
Size: 1598 180 106
Fragments arranged by size:
1598 180 106
MslI CAynn'nnrTG
Cuts at: 0 80 1657 1884
Size: 80 1577 227
Fragments arranged by size:
1577 227 80
MspA1I CmG'CkG
Cuts at: 0 42 117 309 864 1357 1385 1427 1451
Size: 42 75 192 555 493 28 42 24
Cuts at: 1451 1743 1862 1884
Size: 292 119 22
Fragments arranged by size:
555 493 292 192 119 75 42 42
28 24 22
NarI GG'CG_CC
Cuts at: 0 324 1884
Size: 324 1560
PasI CC'CwG_GG
Cuts at: 0 318 1206 1884
Size: 318 888 678
Fragments arranged by size:
888 678 318
PfoI T'CCnGG_A
Cuts at: 0 626 1884
Size: 626 1258
PmlI CAC'GTG
Cuts at: 0 551 798 1884
Size: 551 247 1086
Fragments arranged by size:
1086 551 247
PpuMI rG'GwC_Cy
Cuts at: 0 998 1884
Size: 998 886
PspOMI G'GGCC_C
Cuts at: 0 35 1725 1884
Size: 35 1690 159
Fragments arranged by size:
1690 159 35
PstI C_TGCA'G
Cuts at: 0 1012 1884
Size: 1012 872
PvuII CAG'CTG
Cuts at: 0 309 864 1385 1451 1884
Size: 309 555 521 66 433
Fragments arranged by size:
555 521 433 309 66
RsrII CG'GwC_CG
Cuts at: 0 624 1884
Size: 624 1260
SacI G_AGCT'C
Cuts at: 0 222 263 1097 1884
Size: 222 41 834 787
Fragments arranged by size:
834 787 222 41
SacII CC_GC'GG
Cuts at: 0 43 1428 1884
Size: 43 1385 456
Fragments arranged by size:
1385 456 43
SfcI C'TryA_G
Cuts at: 0 720 1008 1884
Size: 720 288 876
Fragments arranged by size:
876 720 288
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 743 1654 1884
Size: 743 911 230
Fragments arranged by size:
911 743 230
SfoI GGC'GCC
Cuts at: 0 325 1884
Size: 325 1559
SmaI CCC'GGG
Cuts at: 0 286 985 1884
Size: 286 699 899
Fragments arranged by size:
899 699 286
SmlI C'TyrA_G
Cuts at: 0 952 1884
Size: 952 932
SnaBI TAC'GTA
Cuts at: 0 1266 1884
Size: 1266 618
StuI AGG'CCT
Cuts at: 0 634 1276 1884
Size: 634 642 608
Fragments arranged by size:
642 634 608
StyI C'CwwG_G
Cuts at: 0 1190 1332 1370 1884
Size: 1190 142 38 514
Fragments arranged by size:
1190 514 142 38
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 92 704 798 1324 1494 1884
Size: 92 612 94 526 170 390
Fragments arranged by size:
612 526 390 170 94 92
Tth111I GACn'n_nGTC
Cuts at: 0 474 856 1402 1884
Size: 474 382 546 482
Fragments arranged by size:
546 482 474 382
XhoI C'TCGA_G
Cuts at: 0 952 1884
Size: 952 932
XmaI C'CCGG_G
Cuts at: 0 284 983 1884
Size: 284 699 901
Fragments arranged by size:
901 699 284
Enzymes that do cut:
AarI AccI AcuI AflIII AlfI AlwNI ApaI ApaLI
AvaI BanI BanII BbeI BbsI BbvCI BcgI BglI
BlpI Bme1580I BmgBI BplI BpmI Bpu10I BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI BssHII BstAPI
BstXI BstZ17I BtgI BtgZI BtsI EaeI EagI EciI
EcoICRI Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MfeI
MmeI MslI MspA1I NarI PasI PfoI PmlI PpuMI
PspOMI PstI PvuII RsrII SacI SacII SfcI SfiI
SfoI SmaI SmlI SnaBI StuI StyI TaqII Tth111I
XhoI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BciVI BclI BfrBI BglII BmrI BmtI BpuEI BsaBI
BsiWI BsmI BspHI BsrDI BsrGI BssSI BstBI BstEII
BstYI Bsu36I ClaI CspCI DraI DraIII DrdI EarI
EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KpnI MluI MscI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PflMI
PmeI PpiI PshAI PsiI PspXI PsrI PvuI SalI
SanDI SapI SbfI ScaI SexAI SgfI SgrAI SpeI
SphI SrfI SspI SwaI TatI TstI XbaI XcmI
XmnI ZraI