Restriction map for
pF1KB6133
(Linear) (Six-base) MAPSORT of: KIBB6133.seq Check: 5005 from: 1 to: 1770
KIBB6133.orf
With 182 enzymes: SgfI *
July 15, 2010 18:27 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 33 231 844 1641 1770
Size: 33 198 613 797 129
Fragments arranged by size:
797 613 198 129 33
AgeI A'CCGG_T
Cuts at: 0 254 1770
Size: 254 1516
AleI CACnn'nnGTG
Cuts at: 0 100 964 1770
Size: 100 864 806
Fragments arranged by size:
864 806 100
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 637 669 1770
Size: 637 32 1101
Fragments arranged by size:
1101 637 32
AlwNI CAG_nnn'CTG
Cuts at: 0 642 747 1185 1770
Size: 642 105 438 585
Fragments arranged by size:
642 585 438 105
ApoI r'AATT_y
Cuts at: 0 1726 1770
Size: 1726 44
AvrII C'CTAG_G
Cuts at: 0 1623 1770
Size: 1623 147
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 777 810 1770
Size: 777 33 960
Fragments arranged by size:
960 777 33
BanI G'GyrC_C
Cuts at: 0 298 619 1770
Size: 298 321 1151
Fragments arranged by size:
1151 321 298
BanII G_rGCy'C
Cuts at: 0 211 350 1367 1637 1651 1770
Size: 211 139 1017 270 14 119
Fragments arranged by size:
1017 270 211 139 119 14
BbsI GAAGACnn'nnnn_
Cuts at: 0 761 1770
Size: 761 1009
BbvCI CC'TCA_GC
Cuts at: 0 1019 1770
Size: 1019 751
BciVI GTATCCnnnnn_n'
Cuts at: 0 427 1770
Size: 427 1343
BglI GCCn_nnn'nGGC
Cuts at: 0 1124 1770
Size: 1124 646
BglII A'GATC_T
Cuts at: 0 1154 1770
Size: 1154 616
Bme1580I G_kGCm'C
Cuts at: 0 622 1770
Size: 622 1148
BmgBI CAC'GTC
Cuts at: 0 1392 1770
Size: 1392 378
BmrI ACTGGGnnnn_n'
Cuts at: 0 1026 1770
Size: 1026 744
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 293 431 586 664 886 1324 1441 1584
Size: 293 138 155 78 222 438 117 143
Cuts at: 1584 1701 1770
Size: 117 69
Fragments arranged by size:
438 293 222 155 143 138 117 117
78 69
Bpu10I CC'TnA_GC
Cuts at: 0 1019 1770
Size: 1019 751
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1258 1770
Size: 1258 512
BsaBI GATnn'nnATC
Cuts at: 0 223 1770
Size: 223 1547
BsaWI w'CCGG_w
Cuts at: 0 26 254 1169 1770
Size: 26 228 915 601
Fragments arranged by size:
915 601 228 26
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 637 667 1439 1469 1770
Size: 637 30 772 30 301
Fragments arranged by size:
772 637 301 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 514 1054 1111 1321 1734 1737 1770
Size: 514 540 57 210 413 3 33
Fragments arranged by size:
540 514 413 210 57 33 3
BseYI C'CCAG_C
Cuts at: 0 475 1680 1770
Size: 475 1205 90
Fragments arranged by size:
1205 475 90
BsiHKAI G_wGCw'C
Cuts at: 0 1367 1770
Size: 1367 403
BsmI GAATG_Cn'
Cuts at: 0 1438 1770
Size: 1438 332
Bsp1286I G_dGCh'C
Cuts at: 0 211 350 622 1367 1637 1651 1770
Size: 211 139 272 745 270 14 119
Fragments arranged by size:
745 272 270 211 139 119 14
BspEI T'CCGG_A
Cuts at: 0 26 1770
Size: 26 1744
BspHI T'CATG_A
Cuts at: 0 905 1367 1770
Size: 905 462 403
Fragments arranged by size:
905 462 403
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 470 1770
Size: 470 1300
BsrBI CCG'CTC
Cuts at: 0 32 1770
Size: 32 1738
BsrDI GCAATG_nn'
Cuts at: 0 1238 1770
Size: 1238 532
BsrFI r'CCGG_y
Cuts at: 0 254 301 1118 1770
Size: 254 47 817 652
Fragments arranged by size:
817 652 254 47
BstYI r'GATC_y
Cuts at: 0 1154 1770
Size: 1154 616
Bsu36I CC'TnA_GG
Cuts at: 0 676 1770
Size: 676 1094
BtgI C'CryG_G
Cuts at: 0 977 1770
Size: 977 793
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 999 1142 1770
Size: 999 143 628
Fragments arranged by size:
999 628 143
BtsI GCAGTG_nn'
Cuts at: 0 797 1494 1515 1521 1590 1614 1674 1704
Size: 797 697 21 6 69 24 60 30
Cuts at: 1704 1770
Size: 66
Fragments arranged by size:
797 697 69 66 60 30 24 21
6
DraIII CAC_nnn'GTG
Cuts at: 0 596 1770
Size: 596 1174
EaeI y'GGCC_r
Cuts at: 0 1116 1295 1671 1770
Size: 1116 179 376 99
Fragments arranged by size:
1116 376 179 99
EarI CTCTTCn'nnn_
Cuts at: 0 1750 1770
Size: 1750 20
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 371 1770
Size: 371 1399
EcoICRI GAG'CTC
Cuts at: 0 1365 1770
Size: 1365 405
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 33 231 293 431 586 664 844 886
Size: 33 198 62 138 155 78 180 42
Cuts at: 886 1324 1441 1584 1641 1701 1770
Size: 438 117 143 57 60 69
Fragments arranged by size:
438 198 180 155 143 138 117 78
69 62 60 57 42 33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 410 607 1318 1770
Size: 410 197 711 452
Fragments arranged by size:
711 452 410 197
EcoO109I rG'GnC_Cy
Cuts at: 0 744 1311 1770
Size: 744 567 459
Fragments arranged by size:
744 567 459
EcoRV GAT'ATC
Cuts at: 0 221 1770
Size: 221 1549
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 933 1770
Size: 933 837
HincII GTy'rAC
Cuts at: 0 435 1467 1770
Size: 435 1032 303
Fragments arranged by size:
1032 435 303
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1437 1770
Size: 1437 333
MslI CAynn'nnrTG
Cuts at: 0 100 910 964 1127 1770
Size: 100 810 54 163 643
Fragments arranged by size:
810 643 163 100 54
MspA1I CmG'CkG
Cuts at: 0 687 1114 1770
Size: 687 427 656
Fragments arranged by size:
687 656 427
NaeI GCC'GGC
Cuts at: 0 1120 1770
Size: 1120 650
NcoI C'CATG_G
Cuts at: 0 977 1770
Size: 977 793
NgoMIV G'CCGG_C
Cuts at: 0 1118 1770
Size: 1118 652
NspI r_CATG'y
Cuts at: 0 741 952 1770
Size: 741 211 818
Fragments arranged by size:
818 741 211
PfoI T'CCnGG_A
Cuts at: 0 408 563 1750 1770
Size: 408 155 1187 20
Fragments arranged by size:
1187 408 155 20
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 637 669 1770
Size: 637 32 1101
Fragments arranged by size:
1101 637 32
PpuMI rG'GwC_Cy
Cuts at: 0 744 1770
Size: 744 1026
PshAI GACnn'nnGTC
Cuts at: 0 949 1770
Size: 949 821
PstI C_TGCA'G
Cuts at: 0 611 1106 1232 1319 1770
Size: 611 495 126 87 451
Fragments arranged by size:
611 495 451 126 87
PvuII CAG'CTG
Cuts at: 0 687 1114 1770
Size: 687 427 656
Fragments arranged by size:
687 656 427
RsrII CG'GwC_CG
Cuts at: 0 110 1770
Size: 110 1660
SacI G_AGCT'C
Cuts at: 0 1367 1770
Size: 1367 403
SapI GCTCTTCn'nnn_
Cuts at: 0 1750 1770
Size: 1750 20
SexAI A'CCwGG_T
Cuts at: 0 746 1770
Size: 746 1024
SfcI C'TryA_G
Cuts at: 0 607 1102 1228 1315 1770
Size: 607 495 126 87 455
Fragments arranged by size:
607 495 455 126 87
SmlI C'TyrA_G
Cuts at: 0 1273 1770
Size: 1273 497
SpeI A'CTAG_T
Cuts at: 0 232 1770
Size: 232 1538
StyI C'CwwG_G
Cuts at: 0 872 977 1623 1770
Size: 872 105 646 147
Fragments arranged by size:
872 646 147 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 160 511 1770
Size: 160 351 1259
Fragments arranged by size:
1259 351 160
TatI w'GTAC_w
Cuts at: 0 662 1770
Size: 662 1108
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 580 612 1770
Size: 580 32 1158
Fragments arranged by size:
1158 580 32
Tth111I GACn'n_nGTC
Cuts at: 0 1204 1770
Size: 1204 566
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1290 1332 1383 1770
Size: 1290 42 51 387
Fragments arranged by size:
1290 387 51 42
XmnI GAAnn'nnTTC
Cuts at: 0 913 1759 1770
Size: 913 846 11
Fragments arranged by size:
913 846 11
Enzymes that do cut:
AcuI AgeI AleI AloI AlwNI ApoI AvrII BaeI
BanI BanII BbsI BbvCI BciVI BglI BglII Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaBI BsaWI BsaXI
BseRI BseYI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BstYI Bsu36I BtgI BtgZI BtsI
DraIII EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRV FalI HincII MmeI MslI MspA1I NaeI NcoI
NgoMIV NspI PfoI PpiI PpuMI PshAI PstI PvuII
RsrII SacI SapI SexAI SfcI SmlI SpeI StyI
TaqII TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AhdI AlfI ApaI ApaLI AscI AseI AsiSI AvaI
BamHI BbeI BcgI BclI BfrBI BlpI BmtI BplI
BsaI BsaAI BsaHI BsgI BsiEI BsiWI BsmBI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I ClaI
CspCI DraI DrdI EagI EcoRI FseI FspI FspAI
HaeII Hin4I HindIII HpaI KasI KpnI MfeI MluI
MscI NarI NdeI NheI NotI NruI NsiI PacI
PasI PciI PflMI PmeI PmlI PsiI PspOMI PspXI
PsrI PvuI SacII SalI SanDI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SnaBI SphI SrfI SspI
StuI SwaI XbaI XhoI XmaI ZraI