Restriction map for
pF1KB6135
(Linear) (Six-base) MAPSORT of: KIBB6135.seq Check: 523 from: 1 to: 1776
KIBB6135.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AccI GT'mk_AC
Cuts at: 0 584 1776
Size: 584 1192
AclI AA'CG_TT
Cuts at: 0 1142 1776
Size: 1142 634
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1150 1776
Size: 1150 626
AfeI AGC'GCT
Cuts at: 0 409 511 1776
Size: 409 102 1265
Fragments arranged by size:
1265 409 102
AflII C'TTAA_G
Cuts at: 0 691 1776
Size: 691 1085
AflIII A'CryG_T
Cuts at: 0 137 905 1544 1776
Size: 137 768 639 232
Fragments arranged by size:
768 639 232 137
AhdI GACnn_n'nnGTC
Cuts at: 0 968 1536 1776
Size: 968 568 240
Fragments arranged by size:
968 568 240
AleI CACnn'nnGTG
Cuts at: 0 88 1776
Size: 88 1688
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1000 1032 1270 1302 1776
Size: 1000 32 238 32 474
Fragments arranged by size:
1000 474 238 32 32
ApaI G_GGCC'C
Cuts at: 0 1210 1776
Size: 1210 566
ApoI r'AATT_y
Cuts at: 0 827 1776
Size: 827 949
AvaI C'yCGr_G
Cuts at: 0 288 1776
Size: 288 1488
BamHI G'GATC_C
Cuts at: 0 1427 1776
Size: 1427 349
BanII G_rGCy'C
Cuts at: 0 119 1210 1667 1776
Size: 119 1091 457 109
Fragments arranged by size:
1091 457 119 109
BbsI GAAGACnn'nnnn_
Cuts at: 0 335 1776
Size: 335 1441
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1675 1709 1776
Size: 1675 34 67
Fragments arranged by size:
1675 67 34
BglII A'GATC_T
Cuts at: 0 1076 1776
Size: 1076 700
Bme1580I G_kGCm'C
Cuts at: 0 1166 1210 1776
Size: 1166 44 566
Fragments arranged by size:
1166 566 44
BmrI ACTGGGnnnn_n'
Cuts at: 0 894 1575 1776
Size: 894 681 201
Fragments arranged by size:
894 681 201
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 563 1432 1597 1776
Size: 563 869 165 179
Fragments arranged by size:
869 563 179 165
Bpu10I CC'TnA_GC
Cuts at: 0 299 1776
Size: 299 1477
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 157 1776
Size: 37 120 1619
Fragments arranged by size:
1619 120 37
BsaAI yAC'GTr
Cuts at: 0 906 1776
Size: 906 870
BsaBI GATnn'nnATC
Cuts at: 0 1081 1776
Size: 1081 695
BsaHI Gr'CG_yC
Cuts at: 0 95 602 1776
Size: 95 507 1174
Fragments arranged by size:
1174 507 95
BsaWI w'CCGG_w
Cuts at: 0 1424 1776
Size: 1424 352
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1270 1300 1776
Size: 1270 30 476
Fragments arranged by size:
1270 476 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 127 634 1063 1291 1776
Size: 127 507 429 228 485
Fragments arranged by size:
507 485 429 228 127
BseYI C'CCAG_C
Cuts at: 0 4 1308 1776
Size: 4 1304 468
Fragments arranged by size:
1304 468 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 276 459 1084 1690 1776
Size: 133 143 183 625 606 86
Fragments arranged by size:
625 606 183 143 133 86
BsiEI CG_ry'CG
Cuts at: 0 42 1073 1776
Size: 42 1031 703
Fragments arranged by size:
1031 703 42
BsiHKAI G_wGCw'C
Cuts at: 0 119 295 1055 1228 1776
Size: 119 176 760 173 548
Fragments arranged by size:
760 548 176 173 119
Bsp1286I G_dGCh'C
Cuts at: 0 119 295 1055 1166 1210 1228 1667 1776
Size: 119 176 760 111 44 18 439 109
Fragments arranged by size:
760 439 176 119 111 109 44 18
BspEI T'CCGG_A
Cuts at: 0 1424 1776
Size: 1424 352
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 544 1776
Size: 544 1232
BsrBI CCG'CTC
Cuts at: 0 35 165 1776
Size: 35 130 1611
Fragments arranged by size:
1611 130 35
BsrFI r'CCGG_y
Cuts at: 0 13 1508 1776
Size: 13 1495 268
Fragments arranged by size:
1495 268 13
BssSI C'ACGA_G
Cuts at: 0 1102 1776
Size: 1102 674
BstAPI GCAn_nnn'nTGC
Cuts at: 0 488 1232 1776
Size: 488 744 544
Fragments arranged by size:
744 544 488
BstBI TT'CG_AA
Cuts at: 0 1724 1776
Size: 1724 52
BstEII G'GTnAC_C
Cuts at: 0 193 1776
Size: 193 1583
BstXI CCAn_nnnn'nTGG
Cuts at: 0 843 1776
Size: 843 933
BstYI r'GATC_y
Cuts at: 0 1076 1427 1776
Size: 1076 351 349
Fragments arranged by size:
1076 351 349
Bsu36I CC'TnA_GG
Cuts at: 0 1046 1776
Size: 1046 730
BtgI C'CryG_G
Cuts at: 0 516 1776
Size: 516 1260
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1068 1236 1776
Size: 1068 168 540
Fragments arranged by size:
1068 540 168
ClaI AT'CG_AT
Cuts at: 0 704 1776
Size: 704 1072
DraI TTT'AAA
Cuts at: 0 1466 1478 1776
Size: 1466 12 298
Fragments arranged by size:
1466 298 12
EaeI y'GGCC_r
Cuts at: 0 39 387 1070 1334 1510 1776
Size: 39 348 683 264 176 266
Fragments arranged by size:
683 348 266 264 176 39
EagI C'GGCC_G
Cuts at: 0 39 1070 1776
Size: 39 1031 706
Fragments arranged by size:
1031 706 39
EarI CTCTTCn'nnn_
Cuts at: 0 224 400 1776
Size: 224 176 1376
Fragments arranged by size:
1376 224 176
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 37 229 1776
Size: 37 192 1547
Fragments arranged by size:
1547 192 37
EcoICRI GAG'CTC
Cuts at: 0 117 1776
Size: 117 1659
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 563 1150 1432 1597 1776
Size: 563 587 282 165 179
Fragments arranged by size:
587 563 282 179 165
EcoO109I rG'GnC_Cy
Cuts at: 0 456 687 1008 1125 1207 1485 1776
Size: 456 231 321 117 82 278 291
Fragments arranged by size:
456 321 291 278 231 117 82
HaeII r_GCGC'y
Cuts at: 0 411 513 1309 1344 1594 1776
Size: 411 102 796 35 250 182
Fragments arranged by size:
796 411 250 182 102 35
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 904 936 954 986 1522 1554 1776
Size: 904 32 18 32 536 32 222
Fragments arranged by size:
904 536 222 32 32 32 18
HincII GTy'rAC
Cuts at: 0 999 1776
Size: 999 777
MluI A'CGCG_T
Cuts at: 0 1544 1776
Size: 1544 232
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 921 1776
Size: 921 855
MslI CAynn'nnrTG
Cuts at: 0 88 841 1776
Size: 88 753 935
Fragments arranged by size:
935 753 88
MspA1I CmG'CkG
Cuts at: 0 531 1674 1776
Size: 531 1143 102
Fragments arranged by size:
1143 531 102
NaeI GCC'GGC
Cuts at: 0 1510 1776
Size: 1510 266
NdeI CA'TA_TG
Cuts at: 0 548 1776
Size: 548 1228
NgoMIV G'CCGG_C
Cuts at: 0 1508 1776
Size: 1508 268
NotI GC'GGCC_GC
Cuts at: 0 39 1776
Size: 39 1737
NruI TCG'CGA
Cuts at: 0 463 1776
Size: 463 1313
NspI r_CATG'y
Cuts at: 0 141 1026 1191 1776
Size: 141 885 165 585
Fragments arranged by size:
885 585 165 141
PciI A'CATG_T
Cuts at: 0 137 1776
Size: 137 1639
PflMI CCAn_nnn'nTGG
Cuts at: 0 362 1519 1776
Size: 362 1157 257
Fragments arranged by size:
1157 362 257
PfoI T'CCnGG_A
Cuts at: 0 321 652 1148 1409 1776
Size: 321 331 496 261 367
Fragments arranged by size:
496 367 331 321 261
PmlI CAC'GTG
Cuts at: 0 906 1776
Size: 906 870
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1270 1302 1776
Size: 1270 32 474
Fragments arranged by size:
1270 474 32
PpuMI rG'GwC_Cy
Cuts at: 0 687 1008 1125 1485 1776
Size: 687 321 117 360 291
Fragments arranged by size:
687 360 321 291 117
PshAI GACnn'nnGTC
Cuts at: 0 140 1628 1776
Size: 140 1488 148
Fragments arranged by size:
1488 148 140
PspOMI G'GGCC_C
Cuts at: 0 1206 1776
Size: 1206 570
PstI C_TGCA'G
Cuts at: 0 541 749 1776
Size: 541 208 1027
Fragments arranged by size:
1027 541 208
PvuII CAG'CTG
Cuts at: 0 1674 1776
Size: 1674 102
SacI G_AGCT'C
Cuts at: 0 119 1776
Size: 119 1657
SbfI CC_TGCA'GG
Cuts at: 0 541 1776
Size: 541 1235
SfcI C'TryA_G
Cuts at: 0 474 537 745 1119 1776
Size: 474 63 208 374 657
Fragments arranged by size:
657 474 374 208 63
SmlI C'TyrA_G
Cuts at: 0 52 172 691 1776
Size: 52 120 519 1085
Fragments arranged by size:
1085 519 120 52
StuI AGG'CCT
Cuts at: 0 269 1202 1776
Size: 269 933 574
Fragments arranged by size:
933 574 269
StyI C'CwwG_G
Cuts at: 0 791 1776
Size: 791 985
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 168 1776
Size: 168 1608
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 798 1776
Size: 798 978
Enzymes that do cut:
AccI AclI AcuI AfeI AflII AflIII AhdI AleI
AloI ApaI ApoI AvaI BamHI BanII BbsI BcgI
BglII Bme1580I BmrI BpmI Bpu10I BpuEI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspMI BsrBI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI Bsu36I BtgI BtgZI ClaI DraI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I HaeII
Hin4I HincII MluI MmeI MslI MspA1I NaeI NdeI
NgoMIV NotI NruI NspI PciI PflMI PfoI PmlI
PpiI PpuMI PshAI PspOMI PstI PvuII SacI SbfI
SfcI SmlI StuI StyI TaqII XcmI
Enzymes that do not cut:
AarI AatII Acc65I AgeI AlfI AlwNI ApaLI AscI
AseI AsiSI AvrII BaeI BanI BbeI BbvCI BciVI
BclI BfrBI BglI BlpI BmgBI BmtI BplI BsaI
BsiWI BsmI BsmBI BspHI BsrDI BsrGI BssHII BstZ17I
BtsI CspCI DraIII DrdI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HindIII HpaI KasI KpnI MfeI
MscI NarI NcoI NheI NsiI PacI PasI PmeI
PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI