Restriction map for
pF1KB6139
(Linear) (Six-base) MAPSORT of: KIBB6139.seq Check: 9491 from: 1 to: 1779
KIBB6139.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 334 1046 1779
Size: 334 712 733
Fragments arranged by size:
733 712 334
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 1759 1779
Size: 37 1722 20
Fragments arranged by size:
1722 37 20
AhdI GACnn_n'nnGTC
Cuts at: 0 717 1779
Size: 717 1062
AlwNI CAG_nnn'CTG
Cuts at: 0 258 690 987 1110 1779
Size: 258 432 297 123 669
Fragments arranged by size:
669 432 297 258 123
ApaI G_GGCC'C
Cuts at: 0 583 667 1594 1779
Size: 583 84 927 185
Fragments arranged by size:
927 583 185 84
ApoI r'AATT_y
Cuts at: 0 38 1779
Size: 38 1741
AvaI C'yCGr_G
Cuts at: 0 9 331 505 1351 1359 1583 1779
Size: 9 322 174 846 8 224 196
Fragments arranged by size:
846 322 224 196 174 9 8
BanI G'GyrC_C
Cuts at: 0 3 1191 1779
Size: 3 1188 588
Fragments arranged by size:
1188 588 3
BanII G_rGCy'C
Cuts at: 0 235 338 583 667 740 917 1078 1123
Size: 235 103 245 84 73 177 161 45
Cuts at: 1123 1360 1403 1594 1779
Size: 237 43 191 185
Fragments arranged by size:
245 237 235 191 185 177 161 103
84 73 45 43
BbeI G_GCGC'C
Cuts at: 0 7 1779
Size: 7 1772
BbsI GAAGACnn'nnnn_
Cuts at: 0 116 182 388 1779
Size: 116 66 206 1391
Fragments arranged by size:
1391 206 116 66
BbvCI CC'TCA_GC
Cuts at: 0 155 1779
Size: 155 1624
BciVI GTATCCnnnnn_n'
Cuts at: 0 1506 1779
Size: 1506 273
BclI T'GATC_A
Cuts at: 0 260 1779
Size: 260 1519
BfrBI ATG'CAT
Cuts at: 0 882 1779
Size: 882 897
BglI GCCn_nnn'nGGC
Cuts at: 0 12 601 616 637 1513 1779
Size: 12 589 15 21 876 266
Fragments arranged by size:
876 589 266 21 15 12
BlpI GC'TnA_GC
Cuts at: 0 229 811 1779
Size: 229 582 968
Fragments arranged by size:
968 582 229
Bme1580I G_kGCm'C
Cuts at: 0 583 667 1196 1542 1594 1779
Size: 583 84 529 346 52 185
Fragments arranged by size:
583 529 346 185 84 52
BmrI ACTGGGnnnn_n'
Cuts at: 0 200 591 644 1779
Size: 200 391 53 1135
Fragments arranged by size:
1135 391 200 53
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 1342 1353 1399 1779
Size: 232 1110 11 46 380
Fragments arranged by size:
1110 380 232 46 11
Bpu10I CC'TnA_GC
Cuts at: 0 155 1779
Size: 155 1624
BsaI GGTCTCn'nnnn_
Cuts at: 0 793 1779
Size: 793 986
BsaHI Gr'CG_yC
Cuts at: 0 4 1454 1779
Size: 4 1450 325
Fragments arranged by size:
1450 325 4
BsaWI w'CCGG_w
Cuts at: 0 1170 1779
Size: 1170 609
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1075 1105 1325 1355 1779
Size: 1075 30 220 30 424
Fragments arranged by size:
1075 424 220 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 502 1270 1689 1779
Size: 502 768 419 90
Fragments arranged by size:
768 502 419 90
BseYI C'CCAG_C
Cuts at: 0 518 683 1329 1779
Size: 518 165 646 450
Fragments arranged by size:
646 518 450 165
BsiHKAI G_wGCw'C
Cuts at: 0 740 917 1691 1779
Size: 740 177 774 88
Fragments arranged by size:
774 740 177 88
Bsp1286I G_dGCh'C
Cuts at: 0 235 338 583 667 740 917 1078 1123
Size: 235 103 245 84 73 177 161 45
Cuts at: 1123 1196 1360 1403 1542 1594 1691 1779
Size: 73 164 43 139 52 97 88
Fragments arranged by size:
245 235 177 164 161 139 103 97
88 84 73 73 52 45 43
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 334 1046 1779
Size: 334 712 733
Fragments arranged by size:
733 712 334
BsrBI CCG'CTC
Cuts at: 0 47 359 680 1779
Size: 47 312 321 1099
Fragments arranged by size:
1099 321 312 47
BsrDI GCAATG_nn'
Cuts at: 0 1766 1779
Size: 1766 13
BsrFI r'CCGG_y
Cuts at: 0 1373 1466 1779
Size: 1373 93 313
Fragments arranged by size:
1373 313 93
BsrGI T'GTAC_A
Cuts at: 0 1290 1779
Size: 1290 489
BstEII G'GTnAC_C
Cuts at: 0 1656 1779
Size: 1656 123
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1377 1779
Size: 1377 402
BstYI r'GATC_y
Cuts at: 0 983 1208 1779
Size: 983 225 571
Fragments arranged by size:
983 571 225
Bsu36I CC'TnA_GG
Cuts at: 0 919 1779
Size: 919 860
BtgI C'CryG_G
Cuts at: 0 236 752 950 1115 1457 1511 1779
Size: 236 516 198 165 342 54 268
Fragments arranged by size:
516 342 268 236 198 165 54
DraIII CAC_nnn'GTG
Cuts at: 0 536 1779
Size: 536 1243
EaeI y'GGCC_r
Cuts at: 0 560 617 1112 1265 1514 1625 1779
Size: 560 57 495 153 249 111 154
Fragments arranged by size:
560 495 249 154 153 111 57
EcoICRI GAG'CTC
Cuts at: 0 738 915 1779
Size: 738 177 864
Fragments arranged by size:
864 738 177
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 232 1342 1353 1399 1759 1779
Size: 37 195 1110 11 46 360 20
Fragments arranged by size:
1110 360 195 46 37 20 11
EcoNI CCTnn'n_nnAGG
Cuts at: 0 154 1779
Size: 154 1625
EcoO109I rG'GnC_Cy
Cuts at: 0 255 278 579 663 923 1107 1215 1364
Size: 255 23 301 84 260 184 108 149
Cuts at: 1364 1591 1779
Size: 227 188
Fragments arranged by size:
301 260 255 227 188 184 149 108
84 23
EcoRV GAT'ATC
Cuts at: 0 147 1779
Size: 147 1632
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 852 1779
Size: 852 927
FspI TGC'GCA
Cuts at: 0 115 1779
Size: 115 1664
FspAI rTGC'GCAy
Cuts at: 0 115 1779
Size: 115 1664
HaeII r_GCGC'y
Cuts at: 0 7 1779
Size: 7 1772
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 703 735 1325 1357 1779
Size: 703 32 590 32 422
Fragments arranged by size:
703 590 422 32 32
KasI G'GCGC_C
Cuts at: 0 3 1779
Size: 3 1776
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1148 1523 1779
Size: 1148 375 256
Fragments arranged by size:
1148 375 256
MscI TGG'CCA
Cuts at: 0 619 1114 1267 1627 1779
Size: 619 495 153 360 152
Fragments arranged by size:
619 495 360 153 152
MslI CAynn'nnrTG
Cuts at: 0 1286 1779
Size: 1286 493
MspA1I CmG'CkG
Cuts at: 0 687 709 1329 1413 1421 1531 1779
Size: 687 22 620 84 8 110 248
Fragments arranged by size:
687 620 248 110 84 22 8
NaeI GCC'GGC
Cuts at: 0 1375 1779
Size: 1375 404
NarI GG'CG_CC
Cuts at: 0 4 1779
Size: 4 1775
NcoI C'CATG_G
Cuts at: 0 752 1115 1779
Size: 752 363 664
Fragments arranged by size:
752 664 363
NgoMIV G'CCGG_C
Cuts at: 0 1373 1779
Size: 1373 406
NsiI A_TGCA'T
Cuts at: 0 884 1779
Size: 884 895
NspI r_CATG'y
Cuts at: 0 114 882 1285 1779
Size: 114 768 403 494
Fragments arranged by size:
768 494 403 114
PflMI CCAn_nnn'nTGG
Cuts at: 0 753 1779
Size: 753 1026
PfoI T'CCnGG_A
Cuts at: 0 1727 1779
Size: 1727 52
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 325 357 727 759 1779
Size: 325 32 370 32 1020
Fragments arranged by size:
1020 370 325 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 278 923 1107 1215 1364 1779
Size: 278 645 184 108 149 415
Fragments arranged by size:
645 415 278 184 149 108
PshAI GACnn'nnGTC
Cuts at: 0 1429 1779
Size: 1429 350
PspOMI G'GGCC_C
Cuts at: 0 579 663 1590 1779
Size: 579 84 927 189
Fragments arranged by size:
927 579 189 84
PspXI vC'TCGA_Gb
Cuts at: 0 505 1779
Size: 505 1274
PstI C_TGCA'G
Cuts at: 0 551 1779
Size: 551 1228
PvuII CAG'CTG
Cuts at: 0 687 709 1329 1413 1779
Size: 687 22 620 84 366
Fragments arranged by size:
687 620 366 84 22
SacI G_AGCT'C
Cuts at: 0 740 917 1779
Size: 740 177 862
Fragments arranged by size:
862 740 177
SanDI GG'GwC_CC
Cuts at: 0 278 1779
Size: 278 1501
SbfI CC_TGCA'GG
Cuts at: 0 551 1779
Size: 551 1228
SfcI C'TryA_G
Cuts at: 0 547 1779
Size: 547 1232
SfoI GGC'GCC
Cuts at: 0 5 1779
Size: 5 1774
SmaI CCC'GGG
Cuts at: 0 1353 1585 1779
Size: 1353 232 194
Fragments arranged by size:
1353 232 194
SmlI C'TyrA_G
Cuts at: 0 505 1779
Size: 505 1274
SphI G_CATG'C
Cuts at: 0 114 1285 1779
Size: 114 1171 494
Fragments arranged by size:
1171 494 114
SrfI GCCC'GGGC
Cuts at: 0 1585 1779
Size: 1585 194
StyI C'CwwG_G
Cuts at: 0 752 1115 1779
Size: 752 363 664
Fragments arranged by size:
752 664 363
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 119 852 1537 1779
Size: 119 733 685 242
Fragments arranged by size:
733 685 242 119
TatI w'GTAC_w
Cuts at: 0 1290 1779
Size: 1290 489
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 64 96 915 947 1075 1107 1779
Size: 64 32 819 32 128 32 672
Fragments arranged by size:
819 672 128 64 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 207 1779
Size: 207 1572
XhoI C'TCGA_G
Cuts at: 0 505 1779
Size: 505 1274
XmaI C'CCGG_G
Cuts at: 0 1351 1583 1779
Size: 1351 232 196
Fragments arranged by size:
1351 232 196
XmnI GAAnn'nnTTC
Cuts at: 0 38 200 631 1066 1779
Size: 38 162 431 435 713
Fragments arranged by size:
713 435 431 162 38
Enzymes that do cut:
AarI AcuI AhdI AlwNI ApaI ApoI AvaI BanI
BanII BbeI BbsI BbvCI BciVI BclI BfrBI BglI
BlpI Bme1580I BmrI BpmI Bpu10I BsaI BsaHI BsaWI
BsaXI BseRI BseYI BsiHKAI Bsp1286I BspMI BsrBI BsrDI
BsrFI BsrGI BstEII BstXI BstYI Bsu36I BtgI DraIII
EaeI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FalI FspI
FspAI HaeII Hin4I KasI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PflMI PfoI
PpiI PpuMI PshAI PspOMI PspXI PstI PvuII SacI
SanDI SbfI SfcI SfoI SmaI SmlI SphI SrfI
StyI TaqII TatI TstI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BcgI BglII BmgBI BmtI BplI BpuEI
BsaAI BsaBI BsgI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BssHII BssSI BstAPI BstBI BstZ17I BtgZI BtsI
ClaI CspCI DraI DrdI EagI EarI EciI EcoRI
FseI HincII HindIII HpaI KpnI MfeI MluI NdeI
NheI NotI NruI PacI PasI PciI PmeI PmlI
PsiI PsrI PvuI RsrII SacII SalI SapI ScaI
SexAI SfiI SgfI SgrAI SnaBI SpeI SspI StuI
SwaI Tth111I XbaI ZraI