Restriction map for
pF1KB6151
(Linear) (Six-base) MAPSORT of: KIBB6151.seq Check: 1453 from: 1 to: 1788
KIBB6151.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1121 1788
Size: 1121 667
AflIII A'CryG_T
Cuts at: 0 38 1788
Size: 38 1750
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 172 204 1788
Size: 172 32 1584
Fragments arranged by size:
1584 172 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1584 1788
Size: 1584 204
ApaI G_GGCC'C
Cuts at: 0 1562 1788
Size: 1562 226
ApoI r'AATT_y
Cuts at: 0 651 755 799 867 1788
Size: 651 104 44 68 921
Fragments arranged by size:
921 651 104 68 44
BamHI G'GATC_C
Cuts at: 0 394 1788
Size: 394 1394
BanII G_rGCy'C
Cuts at: 0 884 1048 1259 1562 1788
Size: 884 164 211 303 226
Fragments arranged by size:
884 303 226 211 164
BbsI GAAGACnn'nnnn_
Cuts at: 0 1109 1788
Size: 1109 679
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1028 1062 1788
Size: 1028 34 726
Fragments arranged by size:
1028 726 34
BclI T'GATC_A
Cuts at: 0 617 1079 1208 1788
Size: 617 462 129 580
Fragments arranged by size:
617 580 462 129
BglI GCCn_nnn'nGGC
Cuts at: 0 1521 1788
Size: 1521 267
BglII A'GATC_T
Cuts at: 0 426 572 1788
Size: 426 146 1216
Fragments arranged by size:
1216 426 146
BlpI GC'TnA_GC
Cuts at: 0 931 1788
Size: 931 857
Bme1580I G_kGCm'C
Cuts at: 0 1562 1788
Size: 1562 226
BmrI ACTGGGnnnn_n'
Cuts at: 0 1011 1689 1788
Size: 1011 678 99
Fragments arranged by size:
1011 678 99
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1312 1788
Size: 1312 476
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 514 776 1282 1360 1788
Size: 514 262 506 78 428
Fragments arranged by size:
514 506 428 262 78
BsaBI GATnn'nnATC
Cuts at: 0 446 1788
Size: 446 1342
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1126 1156 1788
Size: 1126 30 632
Fragments arranged by size:
1126 632 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 427 892 1549 1585 1788
Size: 427 465 657 36 203
Fragments arranged by size:
657 465 427 203 36
BseYI C'CCAG_C
Cuts at: 0 1499 1788
Size: 1499 289
BsiEI CG_ry'CG
Cuts at: 0 24 1788
Size: 24 1764
BsiHKAI G_wGCw'C
Cuts at: 0 293 884 932 1319 1788
Size: 293 591 48 387 469
Fragments arranged by size:
591 469 387 293 48
BsmI GAATG_Cn'
Cuts at: 0 475 595 764 961 1788
Size: 475 120 169 197 827
Fragments arranged by size:
827 475 197 169 120
Bsp1286I G_dGCh'C
Cuts at: 0 293 884 932 1048 1259 1319 1562 1788
Size: 293 591 48 116 211 60 243 226
Fragments arranged by size:
591 293 243 226 211 116 60 48
BsrBI CCG'CTC
Cuts at: 0 1482 1575 1788
Size: 1482 93 213
Fragments arranged by size:
1482 213 93
BsrDI GCAATG_nn'
Cuts at: 0 1724 1788
Size: 1724 64
BsrFI r'CCGG_y
Cuts at: 0 213 1348 1788
Size: 213 1135 440
Fragments arranged by size:
1135 440 213
BsrGI T'GTAC_A
Cuts at: 0 1463 1788
Size: 1463 325
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1584 1788
Size: 1584 204
BstEII G'GTnAC_C
Cuts at: 0 499 1788
Size: 499 1289
BstXI CCAn_nnnn'nTGG
Cuts at: 0 577 1788
Size: 577 1211
BstYI r'GATC_y
Cuts at: 0 378 394 426 447 572 893 1788
Size: 378 16 32 21 125 321 895
Fragments arranged by size:
895 378 321 125 32 21 16
BtgI C'CryG_G
Cuts at: 0 458 842 989 1124 1445 1788
Size: 458 384 147 135 321 343
Fragments arranged by size:
458 384 343 321 147 135
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 836 1788
Size: 836 952
BtsI GCAGTG_nn'
Cuts at: 0 1582 1788
Size: 1582 206
EaeI y'GGCC_r
Cuts at: 0 21 605 1788
Size: 21 584 1183
Fragments arranged by size:
1183 584 21
EagI C'GGCC_G
Cuts at: 0 21 1788
Size: 21 1767
EarI CTCTTCn'nnn_
Cuts at: 0 622 1788
Size: 622 1166
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1539 1788
Size: 1539 249
EcoICRI GAG'CTC
Cuts at: 0 882 1788
Size: 882 906
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 514 776 1121 1282 1360 1788
Size: 514 262 345 161 78 428
Fragments arranged by size:
514 428 345 262 161 78
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1342 1788
Size: 1342 446
EcoO109I rG'GnC_Cy
Cuts at: 0 834 1539 1558 1559 1788
Size: 834 705 19 1 229
Fragments arranged by size:
834 705 229 19 1
EcoRI G'AATT_C
Cuts at: 0 651 867 1788
Size: 651 216 921
Fragments arranged by size:
921 651 216
FseI GG_CCGG'CC
Cuts at: 0 1352 1788
Size: 1352 436
FspI TGC'GCA
Cuts at: 0 90 1788
Size: 90 1698
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 439 471 1126 1158 1752 1784 1788
Size: 439 32 655 32 594 32 4
Fragments arranged by size:
655 594 439 32 32 32 4
HincII GTy'rAC
Cuts at: 0 1167 1612 1788
Size: 1167 445 176
Fragments arranged by size:
1167 445 176
MfeI C'AATT_G
Cuts at: 0 1067 1788
Size: 1067 721
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1679 1738 1788
Size: 1679 59 50
Fragments arranged by size:
1679 59 50
MscI TGG'CCA
Cuts at: 0 607 1788
Size: 607 1181
MspA1I CmG'CkG
Cuts at: 0 220 369 729 864 1503 1788
Size: 220 149 360 135 639 285
Fragments arranged by size:
639 360 285 220 149 135
NaeI GCC'GGC
Cuts at: 0 215 1350 1788
Size: 215 1135 438
Fragments arranged by size:
1135 438 215
NcoI C'CATG_G
Cuts at: 0 458 1788
Size: 458 1330
NdeI CA'TA_TG
Cuts at: 0 735 1788
Size: 735 1053
NgoMIV G'CCGG_C
Cuts at: 0 213 1348 1788
Size: 213 1135 440
Fragments arranged by size:
1135 440 213
NruI TCG'CGA
Cuts at: 0 62 1788
Size: 62 1726
NspI r_CATG'y
Cuts at: 0 1032 1788
Size: 1032 756
PasI CC'CwG_GG
Cuts at: 0 1555 1788
Size: 1555 233
PflMI CCAn_nnn'nTGG
Cuts at: 0 442 1788
Size: 442 1346
PfoI T'CCnGG_A
Cuts at: 0 77 1788
Size: 77 1711
PpuMI rG'GwC_Cy
Cuts at: 0 834 1539 1788
Size: 834 705 249
Fragments arranged by size:
834 705 249
PspOMI G'GGCC_C
Cuts at: 0 1558 1788
Size: 1558 230
PstI C_TGCA'G
Cuts at: 0 863 876 960 1601 1788
Size: 863 13 84 641 187
Fragments arranged by size:
863 641 187 84 13
PvuII CAG'CTG
Cuts at: 0 220 729 864 1503 1788
Size: 220 509 135 639 285
Fragments arranged by size:
639 509 285 220 135
SacI G_AGCT'C
Cuts at: 0 884 1788
Size: 884 904
SfcI C'TryA_G
Cuts at: 0 859 872 956 1597 1788
Size: 859 13 84 641 191
Fragments arranged by size:
859 641 191 84 13
SpeI A'CTAG_T
Cuts at: 0 1224 1788
Size: 1224 564
SspI AAT'ATT
Cuts at: 0 659 1788
Size: 659 1129
StyI C'CwwG_G
Cuts at: 0 458 527 1647 1788
Size: 458 69 1120 141
Fragments arranged by size:
1120 458 141 69
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 373 1788
Size: 373 1415
TatI w'GTAC_w
Cuts at: 0 416 1463 1788
Size: 416 1047 325
Fragments arranged by size:
1047 416 325
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1702 1788
Size: 1702 86
Enzymes that do cut:
AcuI AflIII AloI AlwNI ApaI ApoI BamHI BanII
BbsI BcgI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI BsaBI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BsrBI BsrDI BsrFI BsrGI BstAPI BstEII
BstXI BstYI BtgI BtgZI BtsI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI
Hin4I HincII MfeI MmeI MscI MspA1I NaeI NcoI
NdeI NgoMIV NruI NspI PasI PflMI PfoI PpuMI
PspOMI PstI PvuII SacI SfcI SpeI SspI StyI
TaqII TatI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BanI BbeI BbvCI BciVI BfrBI BmgBI
BmtI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsgI
BsiWI BsmBI BspEI BspHI BspMI BssHII BssSI BstBI
BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EcoRV
FalI FspAI HaeII HindIII HpaI KasI KpnI MluI
MslI NarI NheI NotI NsiI PacI PciI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SphI SrfI
StuI SwaI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI