Restriction map for
pF1KB6154
(Linear) (Six-base) MAPSORT of: KIBB6154.seq Check: 1673 from: 1 to: 1926
KIBB6154.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1628 1926
Size: 1628 298
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 665 781 916 1424 1736 1926
Size: 397 268 116 135 508 312 190
Fragments arranged by size:
508 397 312 268 190 135 116
AflII C'TTAA_G
Cuts at: 0 1381 1926
Size: 1381 545
AflIII A'CryG_T
Cuts at: 0 1773 1926
Size: 1773 153
ApoI r'AATT_y
Cuts at: 0 1290 1926
Size: 1290 636
AseI AT'TA_AT
Cuts at: 0 1139 1415 1926
Size: 1139 276 511
Fragments arranged by size:
1139 511 276
AvaI C'yCGr_G
Cuts at: 0 246 1327 1926
Size: 246 1081 599
Fragments arranged by size:
1081 599 246
BamHI G'GATC_C
Cuts at: 0 193 1926
Size: 193 1733
BanI G'GyrC_C
Cuts at: 0 715 1336 1926
Size: 715 621 590
Fragments arranged by size:
715 621 590
BanII G_rGCy'C
Cuts at: 0 522 1365 1649 1926
Size: 522 843 284 277
Fragments arranged by size:
843 522 284 277
BbsI GAAGACnn'nnnn_
Cuts at: 0 1907 1926
Size: 1907 19
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 268 302 1318 1352 1926
Size: 268 34 1016 34 574
Fragments arranged by size:
1016 574 268 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 488 1443 1926
Size: 488 955 483
Fragments arranged by size:
955 488 483
BfrBI ATG'CAT
Cuts at: 0 660 1841 1926
Size: 660 1181 85
Fragments arranged by size:
1181 660 85
BglII A'GATC_T
Cuts at: 0 380 1851 1926
Size: 380 1471 75
Fragments arranged by size:
1471 380 75
Bme1580I G_kGCm'C
Cuts at: 0 412 1926
Size: 412 1514
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1349 1615 1819 1926
Size: 1349 266 204 107
Fragments arranged by size:
1349 266 204 107
BsaI GGTCTCn'nnnn_
Cuts at: 0 243 994 1926
Size: 243 751 932
Fragments arranged by size:
932 751 243
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 512 1204 1816 1926
Size: 512 692 612 110
Fragments arranged by size:
692 612 512 110
BsmI GAATG_Cn'
Cuts at: 0 268 744 1473 1843 1926
Size: 268 476 729 370 83
Fragments arranged by size:
729 476 370 268 83
Bsp1286I G_dGCh'C
Cuts at: 0 412 522 1365 1649 1926
Size: 412 110 843 284 277
Fragments arranged by size:
843 412 284 277 110
BspHI T'CATG_A
Cuts at: 0 1110 1926
Size: 1110 816
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1628 1926
Size: 1628 298
BsrDI GCAATG_nn'
Cuts at: 0 1478 1926
Size: 1478 448
BsrGI T'GTAC_A
Cuts at: 0 506 1926
Size: 506 1420
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1158 1926
Size: 1158 768
BstYI r'GATC_y
Cuts at: 0 58 193 380 1851 1926
Size: 58 135 187 1471 75
Fragments arranged by size:
1471 187 135 75 58
BtsI GCAGTG_nn'
Cuts at: 0 286 1926
Size: 286 1640
DraI TTT'AAA
Cuts at: 0 465 1926
Size: 465 1461
DraIII CAC_nnn'GTG
Cuts at: 0 543 1926
Size: 543 1383
EaeI y'GGCC_r
Cuts at: 0 33 1926
Size: 33 1893
EarI CTCTTCn'nnn_
Cuts at: 0 440 1562 1926
Size: 440 1122 364
Fragments arranged by size:
1122 440 364
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 397 665 781 916 1424 1736 1926
Size: 397 268 116 135 508 312 190
Fragments arranged by size:
508 397 312 268 190 135 116
EcoO109I rG'GnC_Cy
Cuts at: 0 243 1155 1926
Size: 243 912 771
Fragments arranged by size:
912 771 243
EcoRI G'AATT_C
Cuts at: 0 1290 1926
Size: 1290 636
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 623 921 1926
Size: 623 298 1005
Fragments arranged by size:
1005 623 298
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 43 75 736 768 1582 1614 1926
Size: 43 32 661 32 814 32 312
Fragments arranged by size:
814 661 312 43 32 32 32
HincII GTy'rAC
Cuts at: 0 208 1638 1926
Size: 208 1430 288
Fragments arranged by size:
1430 288 208
HpaI GTT'AAC
Cuts at: 0 1638 1926
Size: 1638 288
MfeI C'AATT_G
Cuts at: 0 1462 1926
Size: 1462 464
MspA1I CmG'CkG
Cuts at: 0 23 1683 1926
Size: 23 1660 243
Fragments arranged by size:
1660 243 23
NsiI A_TGCA'T
Cuts at: 0 662 1843 1926
Size: 662 1181 83
Fragments arranged by size:
1181 662 83
NspI r_CATG'y
Cuts at: 0 1777 1845 1926
Size: 1777 68 81
Fragments arranged by size:
1777 81 68
PciI A'CATG_T
Cuts at: 0 1773 1926
Size: 1773 153
PflMI CCAn_nnn'nTGG
Cuts at: 0 118 1926
Size: 118 1808
PfoI T'CCnGG_A
Cuts at: 0 196 1926
Size: 196 1730
PpuMI rG'GwC_Cy
Cuts at: 0 243 1155 1926
Size: 243 912 771
Fragments arranged by size:
912 771 243
PstI C_TGCA'G
Cuts at: 0 752 1926
Size: 752 1174
ScaI AGT'ACT
Cuts at: 0 1081 1632 1926
Size: 1081 551 294
Fragments arranged by size:
1081 551 294
SfcI C'TryA_G
Cuts at: 0 748 1054 1784 1926
Size: 748 306 730 142
Fragments arranged by size:
748 730 306 142
SmlI C'TyrA_G
Cuts at: 0 1364 1381 1594 1798 1926
Size: 1364 17 213 204 128
Fragments arranged by size:
1364 213 204 128 17
SpeI A'CTAG_T
Cuts at: 0 181 1695 1926
Size: 181 1514 231
Fragments arranged by size:
1514 231 181
SspI AAT'ATT
Cuts at: 0 1866 1926
Size: 1866 60
StuI AGG'CCT
Cuts at: 0 83 1926
Size: 83 1843
StyI C'CwwG_G
Cuts at: 0 471 628 1151 1601 1926
Size: 471 157 523 450 325
Fragments arranged by size:
523 471 450 325 157
TatI w'GTAC_w
Cuts at: 0 506 1079 1630 1926
Size: 506 573 551 296
Fragments arranged by size:
573 551 506 296
XbaI T'CTAG_A
Cuts at: 0 876 1926
Size: 876 1050
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 510 982 1926
Size: 510 472 944
Fragments arranged by size:
944 510 472
XmnI GAAnn'nnTTC
Cuts at: 0 382 449 1517 1926
Size: 382 67 1068 409
Fragments arranged by size:
1068 409 382 67
Enzymes that do cut:
AarI AcuI AflII AflIII ApoI AseI AvaI BamHI
BanI BanII BbsI BcgI BciVI BfrBI BglII Bme1580I
BpuEI BsaI BseRI BsmI Bsp1286I BspHI BspMI BsrDI
BsrGI BstXI BstYI BtsI DraI DraIII EaeI EarI
Eco57MI EcoO109I EcoRI FalI Hin4I HincII HpaI MfeI
MspA1I NsiI NspI PciI PflMI PfoI PpuMI PstI
ScaI SfcI SmlI SpeI SspI StuI StyI TatI
XbaI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApaLI AscI AsiSI AvrII
BaeI BbeI BbvCI BclI BglI BlpI BmgBI BmrI
BmtI BplI BpmI Bpu10I BsaAI BsaBI BsaHI BsaWI
BsaXI BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI BspEI
BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstZ17I
Bsu36I BtgI BtgZI ClaI CspCI DrdI EagI EciI
EcoICRI EcoNI EcoRV FseI FspI FspAI HaeII HindIII
KasI KpnI MluI MmeI MscI MslI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI PacI PasI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SphI SrfI SwaI TaqII TstI Tth111I XhoI XmaI
ZraI