Restriction map for
pF1KB6157
(Linear) (Six-base) MAPSORT of: KIBB6157.seq Check: 4310 from: 1 to: 1794
KIBB6157.orf
With 182 enzymes: SgfI *
June 26, 2008 10:46 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1686 1794
Size: 1686 108
AccI GT'mk_AC
Cuts at: 0 1176 1215 1794
Size: 1176 39 579
Fragments arranged by size:
1176 579 39
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 598 1013 1457 1794
Size: 598 415 444 337
Fragments arranged by size:
598 444 415 337
AflII C'TTAA_G
Cuts at: 0 871 1794
Size: 871 923
AhdI GACnn_n'nnGTC
Cuts at: 0 1566 1794
Size: 1566 228
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1792 1794
Size: 1792 2
AlwNI CAG_nnn'CTG
Cuts at: 0 21 1794
Size: 21 1773
ApaLI G'TGCA_C
Cuts at: 0 1285 1794
Size: 1285 509
ApoI r'AATT_y
Cuts at: 0 848 1794
Size: 848 946
AvaI C'yCGr_G
Cuts at: 0 814 837 1337 1794
Size: 814 23 500 457
Fragments arranged by size:
814 500 457 23
BanI G'GyrC_C
Cuts at: 0 1679 1705 1794
Size: 1679 26 89
Fragments arranged by size:
1679 89 26
BanII G_rGCy'C
Cuts at: 0 1376 1765 1794
Size: 1376 389 29
Fragments arranged by size:
1376 389 29
BbeI G_GCGC'C
Cuts at: 0 1683 1794
Size: 1683 111
BbsI GAAGACnn'nnnn_
Cuts at: 0 1202 1553 1794
Size: 1202 351 241
Fragments arranged by size:
1202 351 241
BbvCI CC'TCA_GC
Cuts at: 0 1630 1794
Size: 1630 164
BfrBI ATG'CAT
Cuts at: 0 1769 1794
Size: 1769 25
BglI GCCn_nnn'nGGC
Cuts at: 0 770 1794
Size: 770 1024
BglII A'GATC_T
Cuts at: 0 38 1422 1794
Size: 38 1384 372
Fragments arranged by size:
1384 372 38
BlpI GC'TnA_GC
Cuts at: 0 525 1151 1794
Size: 525 626 643
Fragments arranged by size:
643 626 525
Bme1580I G_kGCm'C
Cuts at: 0 1289 1794
Size: 1289 505
BmrI ACTGGGnnnn_n'
Cuts at: 0 617 1794
Size: 617 1177
BmtI G_CTAG'C
Cuts at: 0 733 1794
Size: 733 1061
Bpu10I CC'TnA_GC
Cuts at: 0 1630 1794
Size: 1630 164
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 676 1480 1794
Size: 676 804 314
Fragments arranged by size:
804 676 314
BsaI GGTCTCn'nnnn_
Cuts at: 0 815 1794
Size: 815 979
BsaAI yAC'GTr
Cuts at: 0 1067 1794
Size: 1067 727
BsaHI Gr'CG_yC
Cuts at: 0 1680 1794
Size: 1680 114
BsaWI w'CCGG_w
Cuts at: 0 1465 1794
Size: 1465 329
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 494 524 1794
Size: 494 30 1270
Fragments arranged by size:
1270 494 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 43 73 127 515 569 572 1462 1530
Size: 43 30 54 388 54 3 890 68
Cuts at: 1530 1794
Size: 264
Fragments arranged by size:
890 388 264 68 54 54 43 30
3
BseYI C'CCAG_C
Cuts at: 0 14 735 759 1307 1794
Size: 14 721 24 548 487
Fragments arranged by size:
721 548 487 24 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 129 1794
Size: 129 1665
BsiHKAI G_wGCw'C
Cuts at: 0 846 1289 1794
Size: 846 443 505
Fragments arranged by size:
846 505 443
Bsp1286I G_dGCh'C
Cuts at: 0 846 1289 1376 1765 1794
Size: 846 443 87 389 29
Fragments arranged by size:
846 443 389 87 29
BspEI T'CCGG_A
Cuts at: 0 1465 1794
Size: 1465 329
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 250 1686 1770 1794
Size: 250 1436 84 24
Fragments arranged by size:
1436 250 84 24
BsrDI GCAATG_nn'
Cuts at: 0 1769 1794
Size: 1769 25
BsrFI r'CCGG_y
Cuts at: 0 1109 1734 1794
Size: 1109 625 60
Fragments arranged by size:
1109 625 60
BstEII G'GTnAC_C
Cuts at: 0 510 1794
Size: 510 1284
BstYI r'GATC_y
Cuts at: 0 38 179 1422 1794
Size: 38 141 1243 372
Fragments arranged by size:
1243 372 141 38
Bsu36I CC'TnA_GG
Cuts at: 0 1726 1794
Size: 1726 68
BtsI GCAGTG_nn'
Cuts at: 0 1216 1794
Size: 1216 578
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 234 269 1794
Size: 234 35 1525
Fragments arranged by size:
1525 234 35
EaeI y'GGCC_r
Cuts at: 0 255 1111 1553 1794
Size: 255 856 442 241
Fragments arranged by size:
856 442 255 241
EarI CTCTTCn'nnn_
Cuts at: 0 308 989 1794
Size: 308 681 805
Fragments arranged by size:
805 681 308
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 40 1794
Size: 40 1754
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 598 1013 1457 1794
Size: 598 415 444 337
Fragments arranged by size:
598 444 415 337
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 184 1521 1794
Size: 184 1337 273
Fragments arranged by size:
1337 273 184
HaeII r_GCGC'y
Cuts at: 0 1683 1794
Size: 1683 111
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1552 1584 1794
Size: 1552 32 210
Fragments arranged by size:
1552 210 32
HincII GTy'rAC
Cuts at: 0 413 1794
Size: 413 1381
HindIII A'AGCT_T
Cuts at: 0 713 1794
Size: 713 1081
KasI G'GCGC_C
Cuts at: 0 1679 1794
Size: 1679 115
MscI TGG'CCA
Cuts at: 0 257 1555 1794
Size: 257 1298 239
Fragments arranged by size:
1298 257 239
MslI CAynn'nnrTG
Cuts at: 0 1774 1794
Size: 1774 20
MspA1I CmG'CkG
Cuts at: 0 21 1590 1741 1794
Size: 21 1569 151 53
Fragments arranged by size:
1569 151 53 21
NaeI GCC'GGC
Cuts at: 0 1736 1794
Size: 1736 58
NarI GG'CG_CC
Cuts at: 0 1680 1794
Size: 1680 114
NgoMIV G'CCGG_C
Cuts at: 0 1734 1794
Size: 1734 60
NheI G'CTAG_C
Cuts at: 0 729 1794
Size: 729 1065
NsiI A_TGCA'T
Cuts at: 0 1771 1794
Size: 1771 23
PasI CC'CwG_GG
Cuts at: 0 1675 1794
Size: 1675 119
PflMI CCAn_nnn'nTGG
Cuts at: 0 21 1794
Size: 21 1773
PfoI T'CCnGG_A
Cuts at: 0 891 1142 1794
Size: 891 251 652
Fragments arranged by size:
891 652 251
PstI C_TGCA'G
Cuts at: 0 38 1381 1794
Size: 38 1343 413
Fragments arranged by size:
1343 413 38
PvuII CAG'CTG
Cuts at: 0 21 1590 1794
Size: 21 1569 204
Fragments arranged by size:
1569 204 21
SfcI C'TryA_G
Cuts at: 0 34 1377 1794
Size: 34 1343 417
Fragments arranged by size:
1343 417 34
SfoI GGC'GCC
Cuts at: 0 1681 1794
Size: 1681 113
SmlI C'TyrA_G
Cuts at: 0 691 814 871 1337 1495 1794
Size: 691 123 57 466 158 299
Fragments arranged by size:
691 466 299 158 123 57
SspI AAT'ATT
Cuts at: 0 880 1794
Size: 880 914
TatI w'GTAC_w
Cuts at: 0 1356 1794
Size: 1356 438
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1695 1794
Size: 1695 99
XhoI C'TCGA_G
Cuts at: 0 814 1337 1794
Size: 814 523 457
Fragments arranged by size:
814 523 457
Enzymes that do cut:
AarI AccI AcuI AflII AhdI AlfI AlwNI ApaLI
ApoI AvaI BanI BanII BbeI BbsI BbvCI BfrBI
BglI BglII BlpI Bme1580I BmrI BmtI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspEI BspMI BsrDI BsrFI BstEII BstYI
Bsu36I BtsI CspCI EaeI EarI EciI Eco57MI FalI
HaeII Hin4I HincII HindIII KasI MscI MslI MspA1I
NaeI NarI NgoMIV NheI NsiI PasI PflMI PfoI
PstI PvuII SfcI SfoI SmlI SspI TatI XcmI
XhoI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflIII AgeI AleI AloI
ApaI AscI AseI AsiSI AvrII BaeI BamHI BcgI
BciVI BclI BmgBI BplI BpmI BsaBI BsiEI BsiWI
BsmI BsmBI BspHI BsrBI BsrGI BssHII BssSI BstAPI
BstBI BstXI BstZ17I BtgI BtgZI ClaI DraI DraIII
DrdI EagI EcoICRI EcoNI EcoO109I EcoRI EcoRV FseI
FspI FspAI HpaI KpnI MfeI MluI MmeI NcoI
NdeI NotI NruI NspI PacI PciI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI StuI StyI SwaI TaqII TstI Tth111I XbaI
XmaI XmnI ZraI