Restriction map for
pF1KB6159
(Linear) (Six-base) MAPSORT of: KIBB6159.seq Check: 1333 from: 1 to: 1797
KIBB6159.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AccI GT'mk_AC
Cuts at: 0 867 1797
Size: 867 930
Acc65I G'GTAC_C
Cuts at: 0 177 1797
Size: 177 1620
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 370 1797
Size: 2 368 1427
Fragments arranged by size:
1427 368 2
AflIII A'CryG_T
Cuts at: 0 378 1646 1797
Size: 378 1268 151
Fragments arranged by size:
1268 378 151
AleI CACnn'nnGTG
Cuts at: 0 1127 1797
Size: 1127 670
ApoI r'AATT_y
Cuts at: 0 432 1797
Size: 432 1365
AvaI C'yCGr_G
Cuts at: 0 777 1797
Size: 777 1020
BanI G'GyrC_C
Cuts at: 0 177 1797
Size: 177 1620
BanII G_rGCy'C
Cuts at: 0 644 1797
Size: 644 1153
BbvCI CC'TCA_GC
Cuts at: 0 934 1797
Size: 934 863
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 102 136 1797
Size: 102 34 1661
Fragments arranged by size:
1661 102 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1196 1797
Size: 1196 601
BglII A'GATC_T
Cuts at: 0 389 1266 1797
Size: 389 877 531
Fragments arranged by size:
877 531 389
BlpI GC'TnA_GC
Cuts at: 0 648 1797
Size: 648 1149
BmrI ACTGGGnnnn_n'
Cuts at: 0 34 48 1797
Size: 34 14 1749
Fragments arranged by size:
1749 34 14
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 663 1797
Size: 663 1134
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 1171 1400 1797
Size: 295 876 229 397
Fragments arranged by size:
876 397 295 229
Bpu10I CC'TnA_GC
Cuts at: 0 934 1797
Size: 934 863
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 552 726 1797
Size: 552 174 1071
Fragments arranged by size:
1071 552 174
BsaAI yAC'GTr
Cuts at: 0 1647 1797
Size: 1647 150
BsaWI w'CCGG_w
Cuts at: 0 741 1126 1797
Size: 741 385 671
Fragments arranged by size:
741 671 385
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 533 568 1060 1426 1678 1797
Size: 533 35 492 366 252 119
Fragments arranged by size:
533 492 366 252 119 35
BseYI C'CCAG_C
Cuts at: 0 580 643 1797
Size: 580 63 1154
Fragments arranged by size:
1154 580 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 428 691 915 999 1671 1797
Size: 428 263 224 84 672 126
Fragments arranged by size:
672 428 263 224 126 84
BsmI GAATG_Cn'
Cuts at: 0 574 1797
Size: 574 1223
Bsp1286I G_dGCh'C
Cuts at: 0 644 1797
Size: 644 1153
BsrBI CCG'CTC
Cuts at: 0 1368 1797
Size: 1368 429
BsrGI T'GTAC_A
Cuts at: 0 1611 1797
Size: 1611 186
BssSI C'ACGA_G
Cuts at: 0 1363 1797
Size: 1363 434
BstAPI GCAn_nnn'nTGC
Cuts at: 0 902 1797
Size: 902 895
BstXI CCAn_nnnn'nTGG
Cuts at: 0 449 1797
Size: 449 1348
BstYI r'GATC_y
Cuts at: 0 389 1266 1797
Size: 389 877 531
Fragments arranged by size:
877 531 389
BstZ17I GTA'TAC
Cuts at: 0 868 1797
Size: 868 929
BtsI GCAGTG_nn'
Cuts at: 0 819 903 1659 1797
Size: 819 84 756 138
Fragments arranged by size:
819 756 138 84
DraI TTT'AAA
Cuts at: 0 834 1797
Size: 834 963
EaeI y'GGCC_r
Cuts at: 0 1410 1662 1797
Size: 1410 252 135
Fragments arranged by size:
1410 252 135
EarI CTCTTCn'nnn_
Cuts at: 0 38 223 956 1797
Size: 38 185 733 841
Fragments arranged by size:
841 733 185 38
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 295 370 1171 1400 1797
Size: 2 293 75 801 229 397
Fragments arranged by size:
801 397 293 229 75 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 614 1797
Size: 614 1183
EcoO109I rG'GnC_Cy
Cuts at: 0 782 1797
Size: 782 1015
EcoRI G'AATT_C
Cuts at: 0 432 1797
Size: 432 1365
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1116 1797
Size: 1116 681
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 87 119 222 254 833 865 1081 1113
Size: 87 32 103 32 579 32 216 32
Cuts at: 1113 1797
Size: 684
Fragments arranged by size:
684 579 216 103 87 32 32 32
32
HincII GTy'rAC
Cuts at: 0 376 1181 1349 1601 1797
Size: 376 805 168 252 196
Fragments arranged by size:
805 376 252 196 168
HindIII A'AGCT_T
Cuts at: 0 828 996 1164 1797
Size: 828 168 168 633
Fragments arranged by size:
828 633 168 168
KpnI G_GTAC'C
Cuts at: 0 181 1797
Size: 181 1616
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 953 1405 1797
Size: 953 452 392
Fragments arranged by size:
953 452 392
MslI CAynn'nnrTG
Cuts at: 0 1127 1295 1547 1797
Size: 1127 168 252 250
Fragments arranged by size:
1127 252 250 168
NspI r_CATG'y
Cuts at: 0 382 1797
Size: 382 1415
PasI CC'CwG_GG
Cuts at: 0 442 1797
Size: 442 1355
PciI A'CATG_T
Cuts at: 0 378 1797
Size: 378 1419
PflMI CCAn_nnn'nTGG
Cuts at: 0 64 174 536 753 1797
Size: 64 110 362 217 1044
Fragments arranged by size:
1044 362 217 110 64
PpuMI rG'GwC_Cy
Cuts at: 0 782 1797
Size: 782 1015
PstI C_TGCA'G
Cuts at: 0 1486 1797
Size: 1486 311
SfcI C'TryA_G
Cuts at: 0 726 1482 1797
Size: 726 756 315
Fragments arranged by size:
756 726 315
SmlI C'TyrA_G
Cuts at: 0 567 705 1797
Size: 567 138 1092
Fragments arranged by size:
1092 567 138
TatI w'GTAC_w
Cuts at: 0 381 1611 1797
Size: 381 1230 186
Fragments arranged by size:
1230 381 186
Tth111I GACn'n_nGTC
Cuts at: 0 236 1797
Size: 236 1561
XmnI GAAnn'nnTTC
Cuts at: 0 954 1797
Size: 954 843
Enzymes that do cut:
AccI Acc65I AcuI AflIII AleI ApoI AvaI BanI
BanII BbvCI BcgI BciVI BglII BlpI BmrI BplI
BpmI Bpu10I BpuEI BsaAI BsaWI BseRI BseYI BsgI
BsmI Bsp1286I BsrBI BsrGI BssSI BstAPI BstXI BstYI
BstZ17I BtsI DraI EaeI EarI Eco57MI EcoNI EcoO109I
EcoRI FalI Hin4I HincII HindIII KpnI MmeI MslI
NspI PasI PciI PflMI PpuMI PstI SfcI SmlI
TatI Tth111I XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AlfI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BclI BfrBI BglI Bme1580I
BmgBI BmtI BsaI BsaBI BsaHI BsaXI BsiEI BsiHKAI
BsiWI BsmBI BspEI BspHI BspMI BsrDI BsrFI BssHII
BstBI BstEII Bsu36I BtgI BtgZI ClaI CspCI DraIII
DrdI EagI EciI EcoICRI EcoRV FseI FspI FspAI
HaeII HpaI KasI MfeI MluI MscI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
StyI SwaI TaqII TstI XbaI XcmI XhoI XmaI
ZraI