Restriction map for
pF1KB6163
(Linear) (Six-base) MAPSORT of: KIBB6163.seq Check: 3756 from: 1 to: 1803
KIBB6163.orf
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AccI GT'mk_AC
Cuts at: 0 1525 1803
Size: 1525 278
Acc65I G'GTAC_C
Cuts at: 0 1458 1803
Size: 1458 345
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 368 1803
Size: 368 1435
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1243 1253 1803
Size: 1243 10 550
Fragments arranged by size:
1243 550 10
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 862 894 1803
Size: 862 32 909
Fragments arranged by size:
909 862 32
AlwNI CAG_nnn'CTG
Cuts at: 0 277 391 960 1686 1740 1803
Size: 277 114 569 726 54 63
Fragments arranged by size:
726 569 277 114 63 54
ApaI G_GGCC'C
Cuts at: 0 428 804 1803
Size: 428 376 999
Fragments arranged by size:
999 428 376
ApaLI G'TGCA_C
Cuts at: 0 1072 1803
Size: 1072 731
AvaI C'yCGr_G
Cuts at: 0 890 1027 1388 1531 1803
Size: 890 137 361 143 272
Fragments arranged by size:
890 361 272 143 137
AvrII C'CTAG_G
Cuts at: 0 510 1803
Size: 510 1293
BamHI G'GATC_C
Cuts at: 0 448 1803
Size: 448 1355
BanI G'GyrC_C
Cuts at: 0 1045 1458 1803
Size: 1045 413 345
Fragments arranged by size:
1045 413 345
BanII G_rGCy'C
Cuts at: 0 227 428 523 804 875 983 1003 1792
Size: 227 201 95 281 71 108 20 789
Cuts at: 1792 1803
Size: 11
Fragments arranged by size:
789 281 227 201 108 95 71 20
11
BbsI GAAGACnn'nnnn_
Cuts at: 0 1329 1803
Size: 1329 474
BbvCI CC'TCA_GC
Cuts at: 0 315 1503 1803
Size: 315 1188 300
Fragments arranged by size:
1188 315 300
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1240 1274 1803
Size: 1240 34 529
Fragments arranged by size:
1240 529 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1399 1803
Size: 1399 404
BglII A'GATC_T
Cuts at: 0 1166 1803
Size: 1166 637
BlpI GC'TnA_GC
Cuts at: 0 140 1803
Size: 140 1663
Bme1580I G_kGCm'C
Cuts at: 0 428 804 1048 1076 1803
Size: 428 376 244 28 727
Fragments arranged by size:
727 428 376 244 28
BmrI ACTGGGnnnn_n'
Cuts at: 0 449 1327 1803
Size: 449 878 476
Fragments arranged by size:
878 476 449
BmtI G_CTAG'C
Cuts at: 0 1115 1803
Size: 1115 688
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 138 1000 1386 1803
Size: 138 862 386 417
Fragments arranged by size:
862 417 386 138
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 848 1017 1144 1708 1803
Size: 848 169 127 564 95
Fragments arranged by size:
848 564 169 127 95
Bpu10I CC'TnA_GC
Cuts at: 0 315 805 1503 1803
Size: 315 490 698 300
Fragments arranged by size:
698 490 315 300
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 248 835 1803
Size: 166 82 587 968
Fragments arranged by size:
968 587 166 82
BsaHI Gr'CG_yC
Cuts at: 0 1660 1803
Size: 1660 143
BsaWI w'CCGG_w
Cuts at: 0 1033 1048 1803
Size: 1033 15 755
Fragments arranged by size:
1033 755 15
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 399 402 429 432 862 892 1557 1587
Size: 399 3 27 3 430 30 665 30
Cuts at: 1587 1803
Size: 216
Fragments arranged by size:
665 430 399 216 30 30 27 3
3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 423 1147 1803
Size: 423 724 656
Fragments arranged by size:
724 656 423
BseYI C'CCAG_C
Cuts at: 0 356 439 501 953 1181 1462 1494 1595
Size: 356 83 62 452 228 281 32 101
Cuts at: 1595 1785 1793 1803
Size: 190 8 10
Fragments arranged by size:
452 356 281 228 190 101 83 62
32 10 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1675 1803
Size: 1675 128
BsiEI CG_ry'CG
Cuts at: 0 90 1803
Size: 90 1713
BsiHKAI G_wGCw'C
Cuts at: 0 1076 1803
Size: 1076 727
BsmBI CGTCTCn'nnnn_
Cuts at: 0 16 1803
Size: 16 1787
Bsp1286I G_dGCh'C
Cuts at: 0 227 428 523 804 875 983 1003 1048
Size: 227 201 95 281 71 108 20 45
Cuts at: 1048 1076 1792 1803
Size: 28 716 11
Fragments arranged by size:
716 281 227 201 108 95 71 45
28 20 11
BspHI T'CATG_A
Cuts at: 0 731 1803
Size: 731 1072
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1739 1803
Size: 1739 64
BsrBI CCG'CTC
Cuts at: 0 14 690 1284 1803
Size: 14 676 594 519
Fragments arranged by size:
676 594 519 14
BsrDI GCAATG_nn'
Cuts at: 0 1402 1803
Size: 1402 401
BsrGI T'GTAC_A
Cuts at: 0 854 1803
Size: 854 949
BstAPI GCAn_nnn'nTGC
Cuts at: 0 552 1350 1404 1608 1803
Size: 552 798 54 204 195
Fragments arranged by size:
798 552 204 195 54
BstEII G'GTnAC_C
Cuts at: 0 42 1803
Size: 42 1761
BstXI CCAn_nnnn'nTGG
Cuts at: 0 534 1803
Size: 534 1269
BstYI r'GATC_y
Cuts at: 0 176 267 448 1166 1412 1803
Size: 176 91 181 718 246 391
Fragments arranged by size:
718 391 246 181 176 91
BtgI C'CryG_G
Cuts at: 0 1703 1803
Size: 1703 100
BtsI GCAGTG_nn'
Cuts at: 0 281 381 402 420 1803
Size: 281 100 21 18 1383
Fragments arranged by size:
1383 281 100 21 18
EaeI y'GGCC_r
Cuts at: 0 659 926 1803
Size: 659 267 877
Fragments arranged by size:
877 659 267
EarI CTCTTCn'nnn_
Cuts at: 0 387 924 1291 1803
Size: 387 537 367 512
Fragments arranged by size:
537 512 387 367
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 835 1309 1803
Size: 19 816 474 494
Fragments arranged by size:
816 494 474 19
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 368 848 1017 1144 1708 1803
Size: 368 480 169 127 564 95
Fragments arranged by size:
564 480 368 169 127 95
EcoNI CCTnn'n_nnAGG
Cuts at: 0 613 1803
Size: 613 1190
EcoO109I rG'GnC_Cy
Cuts at: 0 29 424 425 801 1023 1446 1536 1737
Size: 29 395 1 376 222 423 90 201
Cuts at: 1737 1803
Size: 66
Fragments arranged by size:
423 395 376 222 201 90 66 29
1
EcoRV GAT'ATC
Cuts at: 0 1484 1803
Size: 1484 319
FspI TGC'GCA
Cuts at: 0 1219 1803
Size: 1219 584
HaeII r_GCGC'y
Cuts at: 0 1595 1803
Size: 1595 208
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 72 79 83 104 111 115 298 330
Size: 72 7 4 21 7 4 183 32
Cuts at: 330 558 571 590 603 610 642 931 957
Size: 228 13 19 13 7 32 289 26
Cuts at: 957 963 989 1745 1777 1803
Size: 6 26 756 32 26
Fragments arranged by size:
756 289 228 183 72 32 32 32
26 26 26 21 19 13 13 7
7 7 6 4 4
KpnI G_GTAC'C
Cuts at: 0 1462 1803
Size: 1462 341
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 962 1600 1803
Size: 962 638 203
Fragments arranged by size:
962 638 203
MscI TGG'CCA
Cuts at: 0 661 1803
Size: 661 1142
MslI CAynn'nnrTG
Cuts at: 0 655 1803
Size: 655 1148
MspA1I CmG'CkG
Cuts at: 0 153 274 558 564 1185 1686 1803
Size: 153 121 284 6 621 501 117
Fragments arranged by size:
621 501 284 153 121 117 6
NcoI C'CATG_G
Cuts at: 0 1703 1803
Size: 1703 100
NheI G'CTAG_C
Cuts at: 0 1111 1803
Size: 1111 692
NspI r_CATG'y
Cuts at: 0 714 855 1218 1803
Size: 714 141 363 585
Fragments arranged by size:
714 585 363 141
PasI CC'CwG_GG
Cuts at: 0 1450 1803
Size: 1450 353
PflMI CCAn_nnn'nTGG
Cuts at: 0 1629 1803
Size: 1629 174
PfoI T'CCnGG_A
Cuts at: 0 124 614 866 1486 1803
Size: 124 490 252 620 317
Fragments arranged by size:
620 490 317 252 124
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 862 894 1803
Size: 862 32 909
Fragments arranged by size:
909 862 32
PspOMI G'GGCC_C
Cuts at: 0 424 800 1803
Size: 424 376 1003
Fragments arranged by size:
1003 424 376
PstI C_TGCA'G
Cuts at: 0 545 1310 1328 1346 1412 1736 1803
Size: 545 765 18 18 66 324 67
Fragments arranged by size:
765 545 324 67 66 18 18
PvuI CG_AT'CG
Cuts at: 0 90 1803
Size: 90 1713
PvuII CAG'CTG
Cuts at: 0 153 274 564 1185 1686 1803
Size: 153 121 290 621 501 117
Fragments arranged by size:
621 501 290 153 121 117
SapI GCTCTTCn'nnn_
Cuts at: 0 1291 1803
Size: 1291 512
SbfI CC_TGCA'GG
Cuts at: 0 1736 1803
Size: 1736 67
SfcI C'TryA_G
Cuts at: 0 541 1306 1324 1342 1408 1426 1732 1803
Size: 541 765 18 18 66 18 306 71
Fragments arranged by size:
765 541 306 71 66 18 18 18
SmaI CCC'GGG
Cuts at: 0 892 1029 1803
Size: 892 137 774
Fragments arranged by size:
892 774 137
SmlI C'TyrA_G
Cuts at: 0 181 263 814 1803
Size: 181 82 551 989
Fragments arranged by size:
989 551 181 82
StuI AGG'CCT
Cuts at: 0 515 1803
Size: 515 1288
StyI C'CwwG_G
Cuts at: 0 59 510 1467 1703 1803
Size: 59 451 957 236 100
Fragments arranged by size:
957 451 236 100 59
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 87 1456 1803
Size: 87 1369 347
Fragments arranged by size:
1369 347 87
TatI w'GTAC_w
Cuts at: 0 854 1803
Size: 854 949
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 399 402 431 434 1358 1390 1557 1589
Size: 399 3 29 3 924 32 167 32
Cuts at: 1589 1803
Size: 214
Fragments arranged by size:
924 399 214 167 32 32 29 3
3
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1630 1803
Size: 1630 173
XmaI C'CCGG_G
Cuts at: 0 890 1027 1803
Size: 890 137 776
Fragments arranged by size:
890 776 137
Enzymes that do cut:
AccI Acc65I AcuI AlfI AloI AlwNI ApaI ApaLI
AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI
BglI BglII BlpI Bme1580I BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrGI BstAPI BstEII BstXI BstYI BtgI BtsI EaeI
EarI EciI Eco57MI EcoNI EcoO109I EcoRV FspI HaeII
Hin4I KpnI MmeI MscI MslI MspA1I NcoI NheI
NspI PasI PflMI PfoI PpiI PspOMI PstI PvuI
PvuII SapI SbfI SfcI SmaI SmlI StuI StyI
TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AleI ApoI AscI AseI AsiSI BaeI BbeI BciVI
BclI BfrBI BmgBI BsaI BsaAI BsaBI BsiWI BsmI
BspEI BsrFI BssHII BssSI BstBI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoRI
FalI FseI FspAI HincII HindIII HpaI KasI MfeI
MluI NaeI NarI NdeI NgoMIV NotI NruI NsiI
PacI PciI PmeI PmlI PpuMI PshAI PsiI PspXI
PsrI RsrII SacI SacII SalI SanDI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI Tth111I XbaI XhoI XmnI ZraI