Restriction map for
pF1KB6164
(Linear) (Six-base) MAPSORT of: KIBB6164.seq Check: 2922 from: 1 to: 1806
KIBB6164.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AatII G_ACGT'C
Cuts at: 0 1169 1806
Size: 1169 637
AccI GT'mk_AC
Cuts at: 0 1013 1806
Size: 1013 793
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1429 1660 1806
Size: 1429 231 146
Fragments arranged by size:
1429 231 146
AflIII A'CryG_T
Cuts at: 0 1733 1806
Size: 1733 73
AhdI GACnn_n'nnGTC
Cuts at: 0 393 1806
Size: 393 1413
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1120 1152 1193 1225 1806
Size: 1120 32 41 32 581
Fragments arranged by size:
1120 581 41 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 574 1437 1638 1806
Size: 574 863 201 168
Fragments arranged by size:
863 574 201 168
ApaI G_GGCC'C
Cuts at: 0 233 1806
Size: 233 1573
ApoI r'AATT_y
Cuts at: 0 418 1806
Size: 418 1388
AvaI C'yCGr_G
Cuts at: 0 69 755 1319 1806
Size: 69 686 564 487
Fragments arranged by size:
686 564 487 69
BamHI G'GATC_C
Cuts at: 0 750 1806
Size: 750 1056
BanII G_rGCy'C
Cuts at: 0 32 233 602 815 899 1572 1806
Size: 32 201 369 213 84 673 234
Fragments arranged by size:
673 369 234 213 201 84 32
BbvCI CC'TCA_GC
Cuts at: 0 280 1022 1622 1806
Size: 280 742 600 184
Fragments arranged by size:
742 600 280 184
BciVI GTATCCnnnnn_n'
Cuts at: 0 1565 1806
Size: 1565 241
BlpI GC'TnA_GC
Cuts at: 0 205 893 1806
Size: 205 688 913
Fragments arranged by size:
913 688 205
Bme1580I G_kGCm'C
Cuts at: 0 233 1806
Size: 233 1573
BmgBI CAC'GTC
Cuts at: 0 1734 1806
Size: 1734 72
BmrI ACTGGGnnnn_n'
Cuts at: 0 138 1545 1806
Size: 138 1407 261
Fragments arranged by size:
1407 261 138
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 613 922 1072 1171 1774 1806
Size: 613 309 150 99 603 32
Fragments arranged by size:
613 603 309 150 99 32
Bpu10I CC'TnA_GC
Cuts at: 0 280 1022 1622 1806
Size: 280 742 600 184
Fragments arranged by size:
742 600 280 184
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 682 757 1081 1806
Size: 682 75 324 725
Fragments arranged by size:
725 682 324 75
BsaI GGTCTCn'nnnn_
Cuts at: 0 1603 1806
Size: 1603 203
BsaHI Gr'CG_yC
Cuts at: 0 1166 1806
Size: 1166 640
BsaWI w'CCGG_w
Cuts at: 0 539 1806
Size: 539 1267
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 334 364 710 740 1120 1150 1342 1372
Size: 334 30 346 30 380 30 192 30
Cuts at: 1372 1806
Size: 434
Fragments arranged by size:
434 380 346 334 192 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 115 255 358 1168 1543 1806
Size: 115 140 103 810 375 263
Fragments arranged by size:
810 375 263 140 115 103
BseYI C'CCAG_C
Cuts at: 0 107 119 125 131 175 1806
Size: 107 12 6 6 44 1631
Fragments arranged by size:
1631 107 44 12 6 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1328 1434 1806
Size: 1328 106 372
Fragments arranged by size:
1328 372 106
BsiHKAI G_wGCw'C
Cuts at: 0 899 956 1266 1806
Size: 899 57 310 540
Fragments arranged by size:
899 540 310 57
BsmBI CGTCTCn'nnnn_
Cuts at: 0 862 1806
Size: 862 944
Bsp1286I G_dGCh'C
Cuts at: 0 32 233 602 815 899 956 1266 1572
Size: 32 201 369 213 84 57 310 306
Cuts at: 1572 1806
Size: 234
Fragments arranged by size:
369 310 306 234 213 201 84 57
32
BspEI T'CCGG_A
Cuts at: 0 539 1806
Size: 539 1267
BspHI T'CATG_A
Cuts at: 0 1292 1806
Size: 1292 514
BsrBI CCG'CTC
Cuts at: 0 339 762 1806
Size: 339 423 1044
Fragments arranged by size:
1044 423 339
BsrFI r'CCGG_y
Cuts at: 0 1077 1484 1806
Size: 1077 407 322
Fragments arranged by size:
1077 407 322
BsrGI T'GTAC_A
Cuts at: 0 1736 1806
Size: 1736 70
BssSI C'ACGA_G
Cuts at: 0 634 1806
Size: 634 1172
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1261 1806
Size: 1261 545
BstYI r'GATC_y
Cuts at: 0 750 905 1806
Size: 750 155 901
Fragments arranged by size:
901 750 155
Bsu36I CC'TnA_GG
Cuts at: 0 506 1806
Size: 506 1300
BtgI C'CryG_G
Cuts at: 0 1386 1806
Size: 1386 420
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 772 1806
Size: 772 1034
BtsI GCAGTG_nn'
Cuts at: 0 1579 1806
Size: 1579 227
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 63 98 1806
Size: 63 35 1708
Fragments arranged by size:
1708 63 35
DraIII CAC_nnn'GTG
Cuts at: 0 409 1806
Size: 409 1397
EaeI y'GGCC_r
Cuts at: 0 619 1220 1806
Size: 619 601 586
Fragments arranged by size:
619 601 586
EarI CTCTTCn'nnn_
Cuts at: 0 541 944 1213 1502 1636 1769 1806
Size: 541 403 269 289 134 133 37
Fragments arranged by size:
541 403 289 269 134 133 37
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1201 1786 1806
Size: 1201 585 20
Fragments arranged by size:
1201 585 20
EcoICRI GAG'CTC
Cuts at: 0 897 1806
Size: 897 909
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 613 922 1072 1171 1429 1660 1774 1806
Size: 613 309 150 99 258 231 114 32
Fragments arranged by size:
613 309 258 231 150 114 99 32
EcoNI CCTnn'n_nnAGG
Cuts at: 0 991 1054 1806
Size: 991 63 752
Fragments arranged by size:
991 752 63
EcoO109I rG'GnC_Cy
Cuts at: 0 229 230 1806
Size: 229 1 1576
Fragments arranged by size:
1576 229 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 379 411 861 893 1806
Size: 379 32 450 32 913
Fragments arranged by size:
913 450 379 32 32
HindIII A'AGCT_T
Cuts at: 0 304 1806
Size: 304 1502
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 188 1697 1806
Size: 188 1509 109
Fragments arranged by size:
1509 188 109
MscI TGG'CCA
Cuts at: 0 621 1222 1806
Size: 621 601 584
Fragments arranged by size:
621 601 584
MslI CAynn'nnrTG
Cuts at: 0 476 1806
Size: 476 1330
MspA1I CmG'CkG
Cuts at: 0 1026 1218 1621 1806
Size: 1026 192 403 185
Fragments arranged by size:
1026 403 192 185
NcoI C'CATG_G
Cuts at: 0 1386 1806
Size: 1386 420
PfoI T'CCnGG_A
Cuts at: 0 899 1049 1806
Size: 899 150 757
Fragments arranged by size:
899 757 150
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1120 1152 1806
Size: 1120 32 654
Fragments arranged by size:
1120 654 32
PspOMI G'GGCC_C
Cuts at: 0 229 1806
Size: 229 1577
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1270 1302 1546 1578 1806
Size: 1270 32 244 32 228
Fragments arranged by size:
1270 244 228 32 32
PstI C_TGCA'G
Cuts at: 0 302 1806
Size: 302 1504
PvuII CAG'CTG
Cuts at: 0 1026 1218 1621 1806
Size: 1026 192 403 185
Fragments arranged by size:
1026 403 192 185
SacI G_AGCT'C
Cuts at: 0 899 1806
Size: 899 907
SapI GCTCTTCn'nnn_
Cuts at: 0 944 1806
Size: 944 862
ScaI AGT'ACT
Cuts at: 0 220 1806
Size: 220 1586
SfcI C'TryA_G
Cuts at: 0 298 852 1350 1806
Size: 298 554 498 456
Fragments arranged by size:
554 498 456 298
SmlI C'TyrA_G
Cuts at: 0 661 755 772 1096 1806
Size: 661 94 17 324 710
Fragments arranged by size:
710 661 324 94 17
StyI C'CwwG_G
Cuts at: 0 35 1124 1386 1806
Size: 35 1089 262 420
Fragments arranged by size:
1089 420 262 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 484 1806
Size: 484 1322
TatI w'GTAC_w
Cuts at: 0 218 461 698 717 1286 1592 1736 1806
Size: 218 243 237 19 569 306 144 70
Fragments arranged by size:
569 306 243 237 218 144 70 19
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1498 1530 1806
Size: 1498 32 276
Fragments arranged by size:
1498 276 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1749 1806
Size: 1749 57
XhoI C'TCGA_G
Cuts at: 0 755 1806
Size: 755 1051
XmnI GAAnn'nnTTC
Cuts at: 0 535 1806
Size: 535 1271
ZraI GAC'GTC
Cuts at: 0 1167 1806
Size: 1167 639
Enzymes that do cut:
AatII AccI AcuI AflIII AhdI AloI AlwNI ApaI
ApoI AvaI BamHI BanII BbvCI BciVI BlpI Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BsrBI BsrFI BsrGI BssSI BstAPI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraIII EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I Hin4I HindIII MmeI MscI
MslI MspA1I NcoI PfoI PpiI PspOMI PsrI PstI
PvuII SacI SapI ScaI SfcI SmlI StyI TaqII
TatI TstI XcmI XhoI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AleI AlfI
ApaLI AscI AseI AsiSI AvrII BaeI BanI BbeI
BbsI BcgI BclI BfrBI BglI BglII BmtI BplI
BsaAI BsaBI BsiEI BsiWI BsmI BspMI BsrDI BssHII
BstBI BstEII BstXI BstZ17I ClaI DraI DrdI EagI
EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII
HpaI KasI KpnI MfeI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PmeI PmlI PpuMI PshAI PsiI PspXI
PvuI RsrII SacII SalI SanDI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI Tth111I XbaI XmaI