Restriction map for
pF1KB6168
(Linear) (Six-base) MAPSORT of: KIBB6168.seq Check: 263 from: 1 to: 1809
KIBB6168.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 958 1468 1809
Size: 958 510 341
Fragments arranged by size:
958 510 341
AccI GT'mk_AC
Cuts at: 0 724 1109 1809
Size: 724 385 700
Fragments arranged by size:
724 700 385
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 841 1492 1809
Size: 841 651 317
Fragments arranged by size:
841 651 317
AleI CACnn'nnGTG
Cuts at: 0 1121 1756 1809
Size: 1121 635 53
Fragments arranged by size:
1121 635 53
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 265 1809
Size: 265 1544
AlwNI CAG_nnn'CTG
Cuts at: 0 16 498 1140 1809
Size: 16 482 642 669
Fragments arranged by size:
669 642 482 16
AvaI C'yCGr_G
Cuts at: 0 658 1064 1809
Size: 658 406 745
Fragments arranged by size:
745 658 406
BamHI G'GATC_C
Cuts at: 0 1203 1809
Size: 1203 606
BanI G'GyrC_C
Cuts at: 0 36 1480 1809
Size: 36 1444 329
Fragments arranged by size:
1444 329 36
BanII G_rGCy'C
Cuts at: 0 90 395 1514 1777 1809
Size: 90 305 1119 263 32
Fragments arranged by size:
1119 305 263 90 32
BbsI GAAGACnn'nnnn_
Cuts at: 0 631 1809
Size: 631 1178
BbvCI CC'TCA_GC
Cuts at: 0 1199 1573 1809
Size: 1199 374 236
Fragments arranged by size:
1199 374 236
BglII A'GATC_T
Cuts at: 0 206 558 1809
Size: 206 352 1251
Fragments arranged by size:
1251 352 206
BlpI GC'TnA_GC
Cuts at: 0 667 1771 1809
Size: 667 1104 38
Fragments arranged by size:
1104 667 38
Bme1580I G_kGCm'C
Cuts at: 0 39 683 1633 1809
Size: 39 644 950 176
Fragments arranged by size:
950 644 176 39
BmgBI CAC'GTC
Cuts at: 0 336 1809
Size: 336 1473
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 436 583 1720 1809
Size: 436 147 1137 89
Fragments arranged by size:
1137 436 147 89
Bpu10I CC'TnA_GC
Cuts at: 0 1055 1199 1573 1809
Size: 1055 144 374 236
Fragments arranged by size:
1055 374 236 144
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 514 1783 1809
Size: 253 261 1269 26
Fragments arranged by size:
1269 261 253 26
BsaI GGTCTCn'nnnn_
Cuts at: 0 413 518 766 1809
Size: 413 105 248 1043
Fragments arranged by size:
1043 413 248 105
BsaWI w'CCGG_w
Cuts at: 0 782 1007 1809
Size: 782 225 802
Fragments arranged by size:
802 782 225
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 277 307 1809
Size: 277 30 1502
Fragments arranged by size:
1502 277 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 444 1207 1717 1809
Size: 444 763 510 92
Fragments arranged by size:
763 510 444 92
BseYI C'CCAG_C
Cuts at: 0 534 985 1000 1809
Size: 534 451 15 809
Fragments arranged by size:
809 534 451 15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 484 1126 1552 1609 1809
Size: 406 78 642 426 57 200
Fragments arranged by size:
642 426 406 200 78 57
BsiEI CG_ry'CG
Cuts at: 0 44 1809
Size: 44 1765
BsiHKAI G_wGCw'C
Cuts at: 0 90 395 1304 1466 1514 1777 1809
Size: 90 305 909 162 48 263 32
Fragments arranged by size:
909 305 263 162 90 48 32
BsmBI CGTCTCn'nnnn_
Cuts at: 0 943 1801 1809
Size: 943 858 8
Fragments arranged by size:
943 858 8
Bsp1286I G_dGCh'C
Cuts at: 0 39 90 395 683 1304 1466 1514 1633
Size: 39 51 305 288 621 162 48 119
Cuts at: 1633 1777 1809
Size: 144 32
Fragments arranged by size:
621 305 288 162 144 119 51 48
39 32
BspEI T'CCGG_A
Cuts at: 0 782 1809
Size: 782 1027
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 506 958 1468 1809
Size: 506 452 510 341
Fragments arranged by size:
510 506 452 341
BsrFI r'CCGG_y
Cuts at: 0 39 105 146 1733 1809
Size: 39 66 41 1587 76
Fragments arranged by size:
1587 76 66 41 39
BstAPI GCAn_nnn'nTGC
Cuts at: 0 16 480 1140 1809
Size: 16 464 660 669
Fragments arranged by size:
669 660 464 16
BstXI CCAn_nnnn'nTGG
Cuts at: 0 993 1809
Size: 993 816
BstYI r'GATC_y
Cuts at: 0 116 206 558 864 1203 1809
Size: 116 90 352 306 339 606
Fragments arranged by size:
606 352 339 306 116 90
BstZ17I GTA'TAC
Cuts at: 0 725 1809
Size: 725 1084
Bsu36I CC'TnA_GG
Cuts at: 0 452 1809
Size: 452 1357
BtgI C'CryG_G
Cuts at: 0 296 348 827 1809
Size: 296 52 479 982
Fragments arranged by size:
982 479 296 52
BtsI GCAGTG_nn'
Cuts at: 0 17 869 1809
Size: 17 852 940
Fragments arranged by size:
940 852 17
EaeI y'GGCC_r
Cuts at: 0 41 830 1727 1784 1809
Size: 41 789 897 57 25
Fragments arranged by size:
897 789 57 41 25
EagI C'GGCC_G
Cuts at: 0 41 1809
Size: 41 1768
EarI CTCTTCn'nnn_
Cuts at: 0 1309 1809
Size: 1309 500
EcoICRI GAG'CTC
Cuts at: 0 88 393 1512 1775 1809
Size: 88 305 1119 263 34
Fragments arranged by size:
1119 305 263 88 34
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 436 583 841 1492 1720 1809
Size: 436 147 258 651 228 89
Fragments arranged by size:
651 436 258 228 147 89
EcoO109I rG'GnC_Cy
Cuts at: 0 63 126 1809
Size: 63 63 1683
Fragments arranged by size:
1683 63 63
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 204 1641 1689 1809
Size: 204 1437 48 120
Fragments arranged by size:
1437 204 120 48
HaeII r_GCGC'y
Cuts at: 0 156 1559 1809
Size: 156 1403 250
Fragments arranged by size:
1403 250 156
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 277 309 688 720 1386 1418 1809
Size: 277 32 379 32 666 32 391
Fragments arranged by size:
666 391 379 277 32 32 32
HincII GTy'rAC
Cuts at: 0 1110 1809
Size: 1110 699
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 368 1809
Size: 368 1441
MscI TGG'CCA
Cuts at: 0 1729 1809
Size: 1729 80
MslI CAynn'nnrTG
Cuts at: 0 1121 1756 1809
Size: 1121 635 53
Fragments arranged by size:
1121 635 53
MspA1I CmG'CkG
Cuts at: 0 1140 1809
Size: 1140 669
NaeI GCC'GGC
Cuts at: 0 148 1735 1809
Size: 148 1587 74
Fragments arranged by size:
1587 148 74
NcoI C'CATG_G
Cuts at: 0 296 1809
Size: 296 1513
NgoMIV G'CCGG_C
Cuts at: 0 146 1733 1809
Size: 146 1587 76
Fragments arranged by size:
1587 146 76
NspI r_CATG'y
Cuts at: 0 1131 1809
Size: 1131 678
PasI CC'CwG_GG
Cuts at: 0 49 1809
Size: 49 1760
PflMI CCAn_nnn'nTGG
Cuts at: 0 1226 1809
Size: 1226 583
PfoI T'CCnGG_A
Cuts at: 0 81 119 413 1604 1697 1809
Size: 81 38 294 1191 93 112
Fragments arranged by size:
1191 294 112 93 81 38
PpuMI rG'GwC_Cy
Cuts at: 0 63 126 1809
Size: 63 63 1683
Fragments arranged by size:
1683 63 63
PstI C_TGCA'G
Cuts at: 0 12 21 1136 1355 1809
Size: 12 9 1115 219 454
Fragments arranged by size:
1115 454 219 12 9
PvuII CAG'CTG
Cuts at: 0 1140 1809
Size: 1140 669
SacI G_AGCT'C
Cuts at: 0 90 395 1514 1777 1809
Size: 90 305 1119 263 32
Fragments arranged by size:
1119 305 263 90 32
SalI G'TCGA_C
Cuts at: 0 1108 1809
Size: 1108 701
SanDI GG'GwC_CC
Cuts at: 0 63 1809
Size: 63 1746
SapI GCTCTTCn'nnn_
Cuts at: 0 1309 1809
Size: 1309 500
SexAI A'CCwGG_T
Cuts at: 0 1537 1809
Size: 1537 272
SfcI C'TryA_G
Cuts at: 0 8 17 1132 1351 1809
Size: 8 9 1115 219 458
Fragments arranged by size:
1115 458 219 9 8
SgrAI Cr'CCGG_yG
Cuts at: 0 105 1809
Size: 105 1704
SmaI CCC'GGG
Cuts at: 0 1066 1809
Size: 1066 743
SmlI C'TyrA_G
Cuts at: 0 268 529 1798 1809
Size: 268 261 1269 11
Fragments arranged by size:
1269 268 261 11
SspI AAT'ATT
Cuts at: 0 1441 1809
Size: 1441 368
StuI AGG'CCT
Cuts at: 0 1804 1809
Size: 1804 5
StyI C'CwwG_G
Cuts at: 0 224 296 967 1266 1809
Size: 224 72 671 299 543
Fragments arranged by size:
671 543 299 224 72
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 701 1230 1809
Size: 701 529 579
Fragments arranged by size:
701 579 529
TatI w'GTAC_w
Cuts at: 0 1355 1809
Size: 1355 454
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1310 1342 1809
Size: 1310 32 467
Fragments arranged by size:
1310 467 32
XmaI C'CCGG_G
Cuts at: 0 1064 1809
Size: 1064 745
Enzymes that do cut:
AarI AccI AcuI AleI AlfI AlwNI AvaI BamHI
BanI BanII BbsI BbvCI BglII BlpI Bme1580I BmgBI
BpmI Bpu10I BpuEI BsaI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrFI
BstAPI BstXI BstYI BstZ17I Bsu36I BtgI BtsI EaeI
EagI EarI EcoICRI Eco57MI EcoO109I FalI HaeII Hin4I
HincII MmeI MscI MslI MspA1I NaeI NcoI NgoMIV
NspI PasI PflMI PfoI PpuMI PstI PvuII SacI
SalI SanDI SapI SexAI SfcI SgrAI SmaI SmlI
SspI StuI StyI TaqII TatI TstI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BbeI BcgI BciVI BclI BfrBI BglI BmrI
BmtI BplI BsaAI BsaBI BsaHI BsiWI BsmI BspHI
BsrBI BsrDI BsrGI BssHII BssSI BstBI BstEII BtgZI
ClaI CspCI DraI DraIII DrdI EciI EcoNI EcoRI
EcoRV FseI FspI FspAI HindIII HpaI KasI KpnI
MfeI MluI NarI NdeI NheI NotI NruI NsiI
PacI PciI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SbfI ScaI SfiI
SfoI SgfI SnaBI SpeI SphI SrfI SwaI Tth111I
XbaI XcmI XhoI XmnI ZraI