Restriction map for
pF1KB6173
(Linear) (Six-base) MAPSORT of: KIBB6173.seq Check: 421 from: 1 to: 1959
KIBB6173.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 781 1959
Size: 781 1178
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 419 870 1446 1959
Size: 419 451 576 513
Fragments arranged by size:
576 513 451 419
AflIII A'CryG_T
Cuts at: 0 1392 1959
Size: 1392 567
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 833 843 1959
Size: 833 10 1116
Fragments arranged by size:
1116 833 10
AlwNI CAG_nnn'CTG
Cuts at: 0 600 1743 1959
Size: 600 1143 216
Fragments arranged by size:
1143 600 216
ApaI G_GGCC'C
Cuts at: 0 916 1959
Size: 916 1043
ApaLI G'TGCA_C
Cuts at: 0 632 1959
Size: 632 1327
ApoI r'AATT_y
Cuts at: 0 233 1017 1328 1400 1496 1959
Size: 233 784 311 72 96 463
Fragments arranged by size:
784 463 311 233 96 72
AvaI C'yCGr_G
Cuts at: 0 524 781 1959
Size: 524 257 1178
Fragments arranged by size:
1178 524 257
AvrII C'CTAG_G
Cuts at: 0 627 1959
Size: 627 1332
BamHI G'GATC_C
Cuts at: 0 288 1959
Size: 288 1671
BanI G'GyrC_C
Cuts at: 0 766 962 1907 1959
Size: 766 196 945 52
Fragments arranged by size:
945 766 196 52
BanII G_rGCy'C
Cuts at: 0 269 916 1642 1810 1894 1959
Size: 269 647 726 168 84 65
Fragments arranged by size:
726 647 269 168 84 65
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 772 806 1959
Size: 772 34 1153
Fragments arranged by size:
1153 772 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 269 1959
Size: 269 1690
BfrBI ATG'CAT
Cuts at: 0 1858 1959
Size: 1858 101
BlpI GC'TnA_GC
Cuts at: 0 1725 1763 1959
Size: 1725 38 196
Fragments arranged by size:
1725 196 38
Bme1580I G_kGCm'C
Cuts at: 0 636 769 916 965 1910 1959
Size: 636 133 147 49 945 49
Fragments arranged by size:
945 636 147 133 49 49
BmrI ACTGGGnnnn_n'
Cuts at: 0 70 422 826 1959
Size: 70 352 404 1133
Fragments arranged by size:
1133 404 352 70
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 253 622 637 970 1240 1694 1959
Size: 151 102 369 15 333 270 454 265
Fragments arranged by size:
454 369 333 270 265 151 102 15
Bpu10I CC'TnA_GC
Cuts at: 0 263 1166 1959
Size: 263 903 793
Fragments arranged by size:
903 793 263
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 224 1056 1959
Size: 224 832 903
Fragments arranged by size:
903 832 224
BsaI GGTCTCn'nnnn_
Cuts at: 0 156 946 975 1674 1959
Size: 156 790 29 699 285
Fragments arranged by size:
790 699 285 156 29
BsaAI yAC'GTr
Cuts at: 0 1393 1959
Size: 1393 566
BsaWI w'CCGG_w
Cuts at: 0 650 1547 1588 1959
Size: 650 897 41 371
Fragments arranged by size:
897 650 371 41
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 214 745 817 865 910 1959
Size: 214 531 72 48 45 1049
Fragments arranged by size:
1049 531 214 72 48 45
BseYI C'CCAG_C
Cuts at: 0 794 909 1048 1736 1959
Size: 794 115 139 688 223
Fragments arranged by size:
794 688 223 139 115
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 437 1959
Size: 437 1522
BsiHKAI G_wGCw'C
Cuts at: 0 269 636 845 1233 1642 1810 1894 1959
Size: 269 367 209 388 409 168 84 65
Fragments arranged by size:
409 388 367 269 209 168 84 65
BsmI GAATG_Cn'
Cuts at: 0 378 1959
Size: 378 1581
Bsp1286I G_dGCh'C
Cuts at: 0 269 636 769 845 916 965 1233 1642
Size: 269 367 133 76 71 49 268 409
Cuts at: 1642 1810 1894 1910 1959
Size: 168 84 16 49
Fragments arranged by size:
409 367 269 268 168 133 84 76
71 49 49 16
BspEI T'CCGG_A
Cuts at: 0 650 1547 1959
Size: 650 897 412
Fragments arranged by size:
897 650 412
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 426 765 781 1959
Size: 426 339 16 1178
Fragments arranged by size:
1178 426 339 16
BsrDI GCAATG_nn'
Cuts at: 0 1449 1959
Size: 1449 510
BsrGI T'GTAC_A
Cuts at: 0 113 1959
Size: 113 1846
BssHII G'CGCG_C
Cuts at: 0 478 1959
Size: 478 1481
BstAPI GCAn_nnn'nTGC
Cuts at: 0 773 1959
Size: 773 1186
BstEII G'GTnAC_C
Cuts at: 0 463 1959
Size: 463 1496
BstYI r'GATC_y
Cuts at: 0 288 380 596 1959
Size: 288 92 216 1363
Fragments arranged by size:
1363 288 216 92
Bsu36I CC'TnA_GG
Cuts at: 0 470 575 848 1031 1744 1959
Size: 470 105 273 183 713 215
Fragments arranged by size:
713 470 273 215 183 105
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 953 1959
Size: 953 1006
BtsI GCAGTG_nn'
Cuts at: 0 634 788 1533 1959
Size: 634 154 745 426
Fragments arranged by size:
745 634 426 154
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1851 1886 1959
Size: 1851 35 73
Fragments arranged by size:
1851 73 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 24 723 1959
Size: 24 699 1236
Fragments arranged by size:
1236 699 24
EaeI y'GGCC_r
Cuts at: 0 217 1959
Size: 217 1742
EarI CTCTTCn'nnn_
Cuts at: 0 71 299 1405 1647 1959
Size: 71 228 1106 242 312
Fragments arranged by size:
1106 312 242 228 71
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1789 1959
Size: 1789 170
EcoICRI GAG'CTC
Cuts at: 0 267 1640 1808 1892 1959
Size: 267 1373 168 84 67
Fragments arranged by size:
1373 267 168 84 67
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 253 419 622 637 870 970 1240
Size: 151 102 166 203 15 233 100 270
Cuts at: 1240 1446 1694 1959
Size: 206 248 265
Fragments arranged by size:
270 265 248 233 206 203 166 151
102 100 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 706 1959
Size: 706 1253
EcoO109I rG'GnC_Cy
Cuts at: 0 336 387 520 531 1598 1709 1959
Size: 336 51 133 11 1067 111 250
Fragments arranged by size:
1067 336 250 133 111 51 11
EcoRI G'AATT_C
Cuts at: 0 233 1496 1959
Size: 233 1263 463
Fragments arranged by size:
1263 463 233
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1212 1959
Size: 1212 747
FspI TGC'GCA
Cuts at: 0 1351 1959
Size: 1351 608
HaeII r_GCGC'y
Cuts at: 0 1298 1763 1851 1959
Size: 1298 465 88 108
Fragments arranged by size:
1298 465 108 88
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 172 204 1959
Size: 172 32 1755
Fragments arranged by size:
1755 172 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 465 1447 1959
Size: 40 425 982 512
Fragments arranged by size:
982 512 425 40
MscI TGG'CCA
Cuts at: 0 219 1959
Size: 219 1740
MslI CAynn'nnrTG
Cuts at: 0 461 1689 1857 1959
Size: 461 1228 168 102
Fragments arranged by size:
1228 461 168 102
MspA1I CmG'CkG
Cuts at: 0 273 1959
Size: 273 1686
NdeI CA'TA_TG
Cuts at: 0 1854 1959
Size: 1854 105
NsiI A_TGCA'T
Cuts at: 0 1860 1959
Size: 1860 99
NspI r_CATG'y
Cuts at: 0 1862 1959
Size: 1862 97
PflMI CCAn_nnn'nTGG
Cuts at: 0 357 693 873 1959
Size: 357 336 180 1086
Fragments arranged by size:
1086 357 336 180
PfoI T'CCnGG_A
Cuts at: 0 599 866 1124 1959
Size: 599 267 258 835
Fragments arranged by size:
835 599 267 258
PmlI CAC'GTG
Cuts at: 0 1393 1959
Size: 1393 566
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 136 168 1959
Size: 136 32 1791
Fragments arranged by size:
1791 136 32
PpuMI rG'GwC_Cy
Cuts at: 0 336 520 1709 1959
Size: 336 184 1189 250
Fragments arranged by size:
1189 336 250 184
PsiI TTA'TAA
Cuts at: 0 506 1492 1959
Size: 506 986 467
Fragments arranged by size:
986 506 467
PspOMI G'GGCC_C
Cuts at: 0 912 1959
Size: 912 1047
PspXI vC'TCGA_Gb
Cuts at: 0 781 1959
Size: 781 1178
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1543 1575 1959
Size: 1543 32 384
Fragments arranged by size:
1543 384 32
PstI C_TGCA'G
Cuts at: 0 750 1959
Size: 750 1209
PvuII CAG'CTG
Cuts at: 0 273 1959
Size: 273 1686
SacI G_AGCT'C
Cuts at: 0 269 1642 1810 1894 1959
Size: 269 1373 168 84 65
Fragments arranged by size:
1373 269 168 84 65
SapI GCTCTTCn'nnn_
Cuts at: 0 1405 1647 1959
Size: 1405 242 312
Fragments arranged by size:
1405 312 242
SbfI CC_TGCA'GG
Cuts at: 0 750 1959
Size: 750 1209
ScaI AGT'ACT
Cuts at: 0 740 1473 1959
Size: 740 733 486
Fragments arranged by size:
740 733 486
SexAI A'CCwGG_T
Cuts at: 0 146 965 1959
Size: 146 819 994
Fragments arranged by size:
994 819 146
SfcI C'TryA_G
Cuts at: 0 138 568 746 1959
Size: 138 430 178 1213
Fragments arranged by size:
1213 430 178 138
SmaI CCC'GGG
Cuts at: 0 526 1959
Size: 526 1433
SmlI C'TyrA_G
Cuts at: 0 239 781 1071 1959
Size: 239 542 290 888
Fragments arranged by size:
888 542 290 239
SphI G_CATG'C
Cuts at: 0 1862 1959
Size: 1862 97
SspI AAT'ATT
Cuts at: 0 1544 1959
Size: 1544 415
StuI AGG'CCT
Cuts at: 0 1879 1959
Size: 1879 80
StyI C'CwwG_G
Cuts at: 0 587 627 1433 1959
Size: 587 40 806 526
Fragments arranged by size:
806 587 526 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 648 689 943 1900 1959
Size: 648 41 254 957 59
Fragments arranged by size:
957 648 254 59 41
TatI w'GTAC_w
Cuts at: 0 113 738 1059 1471 1959
Size: 113 625 321 412 488
Fragments arranged by size:
625 488 412 321 113
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 180 358 1222 1288 1959
Size: 180 178 864 66 671
Fragments arranged by size:
864 671 180 178 66
XhoI C'TCGA_G
Cuts at: 0 781 1959
Size: 781 1178
XmaI C'CCGG_G
Cuts at: 0 524 1959
Size: 524 1435
XmnI GAAnn'nnTTC
Cuts at: 0 740 1459 1959
Size: 740 719 500
Fragments arranged by size:
740 719 500
Enzymes that do cut:
AarI AcuI AflIII AlfI AlwNI ApaI ApaLI ApoI
AvaI AvrII BamHI BanI BanII BcgI BciVI BfrBI
BlpI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI
BsaWI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI
BspMI BsrDI BsrGI BssHII BstAPI BstEII BstYI Bsu36I
BtgZI BtsI CspCI DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
MmeI MscI MslI MspA1I NdeI NsiI NspI PflMI
PfoI PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI
PstI PvuII SacI SapI SbfI ScaI SexAI SfcI
SmaI SmlI SphI SspI StuI StyI TaqII TatI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI AscI AseI AsiSI BaeI BbeI BbsI
BbvCI BclI BglI BglII BmgBI BmtI BplI BsaBI
BsaHI BsaXI BsiEI BsiWI BsmBI BspHI BsrBI BsrFI
BssSI BstBI BstXI BstZ17I BtgI ClaI DraI DraIII
EagI EcoRV FseI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI NaeI NarI NcoI NgoMIV NheI
NotI NruI PacI PasI PciI PmeI PshAI PvuI
RsrII SacII SalI SanDI SfiI SfoI SgfI SgrAI
SnaBI SpeI SrfI SwaI TstI Tth111I XbaI ZraI