Restriction map for
pF1KB6174
(Linear) (Six-base) MAPSORT of: KIBB6174.seq Check: 4773 from: 1 to: 1890
KIBB6174.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 586 1020 1393 1467 1890
Size: 586 434 373 74 423
Fragments arranged by size:
586 434 423 373 74
AccI GT'mk_AC
Cuts at: 0 1037 1664 1890
Size: 1037 627 226
Fragments arranged by size:
1037 627 226
AclI AA'CG_TT
Cuts at: 0 454 1890
Size: 454 1436
AleI CACnn'nnGTG
Cuts at: 0 1786 1890
Size: 1786 104
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1489 1890
Size: 1489 401
AlwNI CAG_nnn'CTG
Cuts at: 0 939 1241 1252 1890
Size: 939 302 11 638
Fragments arranged by size:
939 638 302 11
ApaI G_GGCC'C
Cuts at: 0 37 163 1489 1890
Size: 37 126 1326 401
Fragments arranged by size:
1326 401 126 37
ApoI r'AATT_y
Cuts at: 0 1156 1890
Size: 1156 734
AvaI C'yCGr_G
Cuts at: 0 333 1890
Size: 333 1557
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1823 1856 1890
Size: 1823 33 34
Fragments arranged by size:
1823 34 33
BamHI G'GATC_C
Cuts at: 0 125 1890
Size: 125 1765
BanI G'GyrC_C
Cuts at: 0 430 755 791 920 1196 1316 1479 1638
Size: 430 325 36 129 276 120 163 159
Cuts at: 1638 1890
Size: 252
Fragments arranged by size:
430 325 276 252 163 159 129 120
36
BanII G_rGCy'C
Cuts at: 0 37 112 163 933 974 996 1489 1890
Size: 37 75 51 770 41 22 493 401
Fragments arranged by size:
770 493 401 75 51 41 37 22
BbeI G_GCGC'C
Cuts at: 0 759 924 1200 1890
Size: 759 165 276 690
Fragments arranged by size:
759 690 276 165
BbsI GAAGACnn'nnnn_
Cuts at: 0 563 1890
Size: 563 1327
BbvCI CC'TCA_GC
Cuts at: 0 5 1890
Size: 5 1885
BciVI GTATCCnnnnn_n'
Cuts at: 0 1578 1890
Size: 1578 312
BclI T'GATC_A
Cuts at: 0 1790 1890
Size: 1790 100
BglI GCCn_nnn'nGGC
Cuts at: 0 223 270 1890
Size: 223 47 1620
Fragments arranged by size:
1620 223 47
Bme1580I G_kGCm'C
Cuts at: 0 37 163 1489 1890
Size: 37 126 1326 401
Fragments arranged by size:
1326 401 126 37
BmrI ACTGGGnnnn_n'
Cuts at: 0 1229 1521 1890
Size: 1229 292 369
Fragments arranged by size:
1229 369 292
Bpu10I CC'TnA_GC
Cuts at: 0 5 1890
Size: 5 1885
BsaI GGTCTCn'nnnn_
Cuts at: 0 337 754 1890
Size: 337 417 1136
Fragments arranged by size:
1136 417 337
BsaHI Gr'CG_yC
Cuts at: 0 756 921 1197 1890
Size: 756 165 276 693
Fragments arranged by size:
756 693 276 165
BsaWI w'CCGG_w
Cuts at: 0 647 1890
Size: 647 1243
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 629 659 1368 1398 1513 1543 1890
Size: 629 30 709 30 115 30 347
Fragments arranged by size:
709 629 347 115 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1723 1735 1890
Size: 1723 12 155
Fragments arranged by size:
1723 155 12
BseYI C'CCAG_C
Cuts at: 0 369 932 1300 1890
Size: 369 563 368 590
Fragments arranged by size:
590 563 369 368
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 557 1118 1495 1890
Size: 557 561 377 395
Fragments arranged by size:
561 557 395 377
BsiHKAI G_wGCw'C
Cuts at: 0 477 1534 1890
Size: 477 1057 356
Fragments arranged by size:
1057 477 356
BsmBI CGTCTCn'nnnn_
Cuts at: 0 630 1890
Size: 630 1260
Bsp1286I G_dGCh'C
Cuts at: 0 37 112 163 477 933 974 996 1489
Size: 37 75 51 314 456 41 22 493
Cuts at: 1489 1534 1890
Size: 45 356
Fragments arranged by size:
493 456 356 314 75 51 45 41
37 22
BspEI T'CCGG_A
Cuts at: 0 647 1890
Size: 647 1243
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 586 1020 1393 1467 1890
Size: 586 434 373 74 423
Fragments arranged by size:
586 434 423 373 74
BsrDI GCAATG_nn'
Cuts at: 0 714 1890
Size: 714 1176
BsrFI r'CCGG_y
Cuts at: 0 1070 1890
Size: 1070 820
BstAPI GCAn_nnn'nTGC
Cuts at: 0 206 1890
Size: 206 1684
BstXI CCAn_nnnn'nTGG
Cuts at: 0 996 1534 1890
Size: 996 538 356
Fragments arranged by size:
996 538 356
BstYI r'GATC_y
Cuts at: 0 125 1890
Size: 125 1765
BtgI C'CryG_G
Cuts at: 0 233 1890
Size: 233 1657
BtsI GCAGTG_nn'
Cuts at: 0 937 1890
Size: 937 953
EaeI y'GGCC_r
Cuts at: 0 711 1125 1890
Size: 711 414 765
Fragments arranged by size:
765 711 414
EarI CTCTTCn'nnn_
Cuts at: 0 187 1757 1890
Size: 187 1570 133
Fragments arranged by size:
1570 187 133
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1128 1597 1890
Size: 1128 469 293
Fragments arranged by size:
1128 469 293
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1516 1890
Size: 1516 374
EcoO109I rG'GnC_Cy
Cuts at: 0 34 160 330 566 1091 1486 1890
Size: 34 126 170 236 525 395 404
Fragments arranged by size:
525 404 395 236 170 126 34
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1362 1890
Size: 1362 528
HaeII r_GCGC'y
Cuts at: 0 51 759 924 1182 1200 1890
Size: 51 708 165 258 18 690
Fragments arranged by size:
708 690 258 165 51 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 110 142 592 624 629 661 1726 1758
Size: 110 32 450 32 5 32 1065 32
Cuts at: 1758 1890
Size: 132
Fragments arranged by size:
1065 450 132 110 32 32 32 32
5
KasI G'GCGC_C
Cuts at: 0 755 920 1196 1890
Size: 755 165 276 694
Fragments arranged by size:
755 694 276 165
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 279 1411 1890
Size: 279 1132 479
Fragments arranged by size:
1132 479 279
MscI TGG'CCA
Cuts at: 0 713 1890
Size: 713 1177
MslI CAynn'nnrTG
Cuts at: 0 1786 1890
Size: 1786 104
MspA1I CmG'CkG
Cuts at: 0 444 591 1130 1615 1890
Size: 444 147 539 485 275
Fragments arranged by size:
539 485 444 275 147
NaeI GCC'GGC
Cuts at: 0 1072 1890
Size: 1072 818
NarI GG'CG_CC
Cuts at: 0 756 921 1197 1890
Size: 756 165 276 693
Fragments arranged by size:
756 693 276 165
NgoMIV G'CCGG_C
Cuts at: 0 1070 1890
Size: 1070 820
NspI r_CATG'y
Cuts at: 0 877 1890
Size: 877 1013
PasI CC'CwG_GG
Cuts at: 0 30 1890
Size: 30 1860
PflMI CCAn_nnn'nTGG
Cuts at: 0 234 603 1241 1890
Size: 234 369 638 649
Fragments arranged by size:
649 638 369 234
PspOMI G'GGCC_C
Cuts at: 0 33 159 1485 1890
Size: 33 126 1326 405
Fragments arranged by size:
1326 405 126 33
PstI C_TGCA'G
Cuts at: 0 590 1211 1890
Size: 590 621 679
Fragments arranged by size:
679 621 590
PvuII CAG'CTG
Cuts at: 0 591 1615 1890
Size: 591 1024 275
Fragments arranged by size:
1024 591 275
SfcI C'TryA_G
Cuts at: 0 586 1051 1207 1890
Size: 586 465 156 683
Fragments arranged by size:
683 586 465 156
SfoI GGC'GCC
Cuts at: 0 757 922 1198 1890
Size: 757 165 276 692
Fragments arranged by size:
757 692 276 165
StuI AGG'CCT
Cuts at: 0 1673 1890
Size: 1673 217
StyI C'CwwG_G
Cuts at: 0 221 347 1346 1849 1890
Size: 221 126 999 503 41
Fragments arranged by size:
999 503 221 126 41
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 380 448 1801 1890
Size: 380 68 1353 89
Fragments arranged by size:
1353 380 89 68
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1107 1139 1513 1545 1890
Size: 1107 32 374 32 345
Fragments arranged by size:
1107 374 345 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 706 1890
Size: 706 1184
XmnI GAAnn'nnTTC
Cuts at: 0 59 1890
Size: 59 1831
Enzymes that do cut:
AarI AccI AclI AleI AlfI AlwNI ApaI ApoI
AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI
BciVI BclI BglI Bme1580I BmrI Bpu10I BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I
BspEI BspMI BsrDI BsrFI BstAPI BstXI BstYI BtgI
BtsI EaeI EarI EciI EcoNI EcoO109I FalI HaeII
Hin4I KasI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NspI PasI PflMI PspOMI PstI PvuII SfcI
SfoI StuI StyI TaqII TstI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AcuI AfeI AflII AflIII AgeI AhdI
AloI ApaLI AscI AseI AsiSI AvrII BcgI BfrBI
BglII BlpI BmgBI BmtI BplI BpmI BpuEI BsaAI
BsaBI BsiEI BsiWI BsmI BspHI BsrBI BsrGI BssHII
BssSI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII DrdI EagI EcoICRI Eco57MI EcoRI EcoRV
FseI FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI NcoI NdeI NheI NotI NruI NsiI PacI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI SwaI TatI
Tth111I XbaI XhoI XmaI ZraI