Restriction map for
pF1KB6179
(Linear) (Six-base) MAPSORT of: KIBB6179.seq Check: 4937 from: 1 to: 1821
KIBB6179.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AccI GT'mk_AC
Cuts at: 0 401 1821
Size: 401 1420
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1072 1145 1630 1708 1821
Size: 1072 73 485 78 113
Fragments arranged by size:
1072 485 113 78 73
AflII C'TTAA_G
Cuts at: 0 769 1821
Size: 769 1052
AflIII A'CryG_T
Cuts at: 0 1283 1739 1821
Size: 1283 456 82
Fragments arranged by size:
1283 456 82
AleI CACnn'nnGTG
Cuts at: 0 409 1821
Size: 409 1412
AlwNI CAG_nnn'CTG
Cuts at: 0 763 1659 1785 1821
Size: 763 896 126 36
Fragments arranged by size:
896 763 126 36
ApaI G_GGCC'C
Cuts at: 0 54 1821
Size: 54 1767
AvaI C'yCGr_G
Cuts at: 0 191 229 692 1517 1821
Size: 191 38 463 825 304
Fragments arranged by size:
825 463 304 191 38
BamHI G'GATC_C
Cuts at: 0 1395 1821
Size: 1395 426
BanI G'GyrC_C
Cuts at: 0 6 57 81 1821
Size: 6 51 24 1740
Fragments arranged by size:
1740 51 24 6
BanII G_rGCy'C
Cuts at: 0 54 230 1316 1624 1821
Size: 54 176 1086 308 197
Fragments arranged by size:
1086 308 197 176 54
BbeI G_GCGC'C
Cuts at: 0 10 61 1821
Size: 10 51 1760
Fragments arranged by size:
1760 51 10
BbsI GAAGACnn'nnnn_
Cuts at: 0 511 985 1618 1821
Size: 511 474 633 203
Fragments arranged by size:
633 511 474 203
BciVI GTATCCnnnnn_n'
Cuts at: 0 434 627 1821
Size: 434 193 1194
Fragments arranged by size:
1194 434 193
BclI T'GATC_A
Cuts at: 0 92 1821
Size: 92 1729
BglI GCCn_nnn'nGGC
Cuts at: 0 241 1821
Size: 241 1580
BlpI GC'TnA_GC
Cuts at: 0 1706 1821
Size: 1706 115
Bme1580I G_kGCm'C
Cuts at: 0 54 1176 1821
Size: 54 1122 645
Fragments arranged by size:
1122 645 54
BmgBI CAC'GTC
Cuts at: 0 105 188 1821
Size: 105 83 1633
Fragments arranged by size:
1633 105 83
BmrI ACTGGGnnnn_n'
Cuts at: 0 346 435 723 1821
Size: 346 89 288 1098
Fragments arranged by size:
1098 346 288 89
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1576 1821
Size: 1576 245
Bpu10I CC'TnA_GC
Cuts at: 0 124 140 224 1821
Size: 124 16 84 1597
Fragments arranged by size:
1597 124 84 16
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1714 1821
Size: 1714 107
BsaI GGTCTCn'nnnn_
Cuts at: 0 1486 1821
Size: 1486 335
BsaAI yAC'GTr
Cuts at: 0 301 1742 1821
Size: 301 1441 79
Fragments arranged by size:
1441 301 79
BsaBI GATnn'nnATC
Cuts at: 0 943 1821
Size: 943 878
BsaHI Gr'CG_yC
Cuts at: 0 7 58 1821
Size: 7 51 1763
Fragments arranged by size:
1763 51 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 63 1821
Size: 63 1758
BseYI C'CCAG_C
Cuts at: 0 561 1821
Size: 561 1260
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 1171 1821
Size: 277 894 650
Fragments arranged by size:
894 650 277
BsiEI CG_ry'CG
Cuts at: 0 152 1821
Size: 152 1669
BsiHKAI G_wGCw'C
Cuts at: 0 230 1301 1821
Size: 230 1071 520
Fragments arranged by size:
1071 520 230
Bsp1286I G_dGCh'C
Cuts at: 0 54 230 1176 1301 1316 1624 1821
Size: 54 176 946 125 15 308 197
Fragments arranged by size:
946 308 197 176 125 54 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 249 1431 1821
Size: 249 1182 390
Fragments arranged by size:
1182 390 249
BsrDI GCAATG_nn'
Cuts at: 0 900 1821
Size: 900 921
BsrFI r'CCGG_y
Cuts at: 0 16 1821
Size: 16 1805
BstEII G'GTnAC_C
Cuts at: 0 1036 1788 1821
Size: 1036 752 33
Fragments arranged by size:
1036 752 33
BstYI r'GATC_y
Cuts at: 0 1395 1821
Size: 1395 426
BtsI GCAGTG_nn'
Cuts at: 0 1591 1821
Size: 1591 230
DraI TTT'AAA
Cuts at: 0 642 1821
Size: 642 1179
DrdI GACnn_nn'nnGTC
Cuts at: 0 1273 1495 1821
Size: 1273 222 326
Fragments arranged by size:
1273 326 222
EaeI y'GGCC_r
Cuts at: 0 149 1212 1821
Size: 149 1063 609
Fragments arranged by size:
1063 609 149
EagI C'GGCC_G
Cuts at: 0 149 1821
Size: 149 1672
EarI CTCTTCn'nnn_
Cuts at: 0 275 1543 1821
Size: 275 1268 278
Fragments arranged by size:
1268 278 275
EcoICRI GAG'CTC
Cuts at: 0 228 1821
Size: 228 1593
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1072 1145 1576 1630 1708 1821
Size: 1072 73 431 54 78 113
Fragments arranged by size:
1072 431 113 78 73 54
EcoNI CCTnn'n_nnAGG
Cuts at: 0 139 1821
Size: 139 1682
EcoO109I rG'GnC_Cy
Cuts at: 0 51 585 775 1821
Size: 51 534 190 1046
Fragments arranged by size:
1046 534 190 51
HaeII r_GCGC'y
Cuts at: 0 10 61 71 1713 1779 1821
Size: 10 51 10 1642 66 42
Fragments arranged by size:
1642 66 51 42 10 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1083 1115 1821
Size: 1083 32 706
Fragments arranged by size:
1083 706 32
HincII GTy'rAC
Cuts at: 0 1458 1821
Size: 1458 363
HindIII A'AGCT_T
Cuts at: 0 526 1821
Size: 526 1295
KasI G'GCGC_C
Cuts at: 0 6 57 1821
Size: 6 51 1764
Fragments arranged by size:
1764 51 6
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 451 523 1821
Size: 451 72 1298
Fragments arranged by size:
1298 451 72
MslI CAynn'nnrTG
Cuts at: 0 409 520 685 1821
Size: 409 111 165 1136
Fragments arranged by size:
1136 409 165 111
MspA1I CmG'CkG
Cuts at: 0 90 1821
Size: 90 1731
NaeI GCC'GGC
Cuts at: 0 18 1821
Size: 18 1803
NarI GG'CG_CC
Cuts at: 0 7 58 1821
Size: 7 51 1763
Fragments arranged by size:
1763 51 7
NgoMIV G'CCGG_C
Cuts at: 0 16 1821
Size: 16 1805
PflMI CCAn_nnn'nTGG
Cuts at: 0 814 1785 1809 1821
Size: 814 971 24 12
Fragments arranged by size:
971 814 24 12
PfoI T'CCnGG_A
Cuts at: 0 1808 1821
Size: 1808 13
PpuMI rG'GwC_Cy
Cuts at: 0 585 1821
Size: 585 1236
PshAI GACnn'nnGTC
Cuts at: 0 694 1453 1821
Size: 694 759 368
Fragments arranged by size:
759 694 368
PspOMI G'GGCC_C
Cuts at: 0 50 1821
Size: 50 1771
PspXI vC'TCGA_Gb
Cuts at: 0 229 1821
Size: 229 1592
PstI C_TGCA'G
Cuts at: 0 1310 1601 1821
Size: 1310 291 220
Fragments arranged by size:
1310 291 220
SacI G_AGCT'C
Cuts at: 0 230 1821
Size: 230 1591
SapI GCTCTTCn'nnn_
Cuts at: 0 1543 1821
Size: 1543 278
SfcI C'TryA_G
Cuts at: 0 624 932 1306 1597 1821
Size: 624 308 374 291 224
Fragments arranged by size:
624 374 308 291 224
SfoI GGC'GCC
Cuts at: 0 8 59 1821
Size: 8 51 1762
Fragments arranged by size:
1762 51 8
SmaI CCC'GGG
Cuts at: 0 1519 1821
Size: 1519 302
SmlI C'TyrA_G
Cuts at: 0 229 769 1729 1821
Size: 229 540 960 92
Fragments arranged by size:
960 540 229 92
SspI AAT'ATT
Cuts at: 0 723 1821
Size: 723 1098
StuI AGG'CCT
Cuts at: 0 1472 1573 1821
Size: 1472 101 248
Fragments arranged by size:
1472 248 101
StyI C'CwwG_G
Cuts at: 0 1391 1821
Size: 1391 430
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 421 454 1024 1821
Size: 421 33 570 797
Fragments arranged by size:
797 570 421 33
TatI w'GTAC_w
Cuts at: 0 645 1821
Size: 645 1176
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1380 1412 1821
Size: 1380 32 409
Fragments arranged by size:
1380 409 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 991 1821
Size: 991 830
XhoI C'TCGA_G
Cuts at: 0 229 1821
Size: 229 1592
XmaI C'CCGG_G
Cuts at: 0 1517 1821
Size: 1517 304
Enzymes that do cut:
AccI AcuI AflII AflIII AleI AlwNI ApaI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglI
BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspMI BsrDI BsrFI BstEII BstYI BtsI DraI
DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I
HaeII Hin4I HincII HindIII KasI MmeI MslI MspA1I
NaeI NarI NgoMIV PflMI PfoI PpuMI PshAI PspOMI
PspXI PstI SacI SapI SfcI SfoI SmaI SmlI
SspI StuI StyI TaqII TatI TstI XcmI XhoI
XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AlfI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BbvCI BcgI BfrBI BglII BmtI BplI BsaWI BsaXI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrGI BssHII
BssSI BstAPI BstBI BstXI BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DraIII EciI EcoRI EcoRV FalI FseI
FspI FspAI HpaI KpnI MfeI MluI MscI NcoI
NdeI NheI NotI NruI NsiI NspI PacI PasI
PciI PmeI PmlI PpiI PsiI PsrI PvuI PvuII
RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI
SgfI SgrAI SnaBI SpeI SphI SrfI SwaI Tth111I
XbaI XmnI ZraI