Restriction map for
pF1KB6184
(Linear) (Six-base) MAPSORT of: KIBB6184.seq Check: 9537 from: 1 to: 2100
KIBB6184.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1974 2100
Size: 1974 126
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 947 1208 1615 2083 2100
Size: 358 589 261 407 468 17
Fragments arranged by size:
589 468 407 358 261 17
AleI CACnn'nnGTG
Cuts at: 0 1429 2100
Size: 1429 671
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 148 180 2100
Size: 148 32 1920
Fragments arranged by size:
1920 148 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1368 1985 2052 2100
Size: 1368 617 67 48
Fragments arranged by size:
1368 617 67 48
ApaI G_GGCC'C
Cuts at: 0 132 2100
Size: 132 1968
ApoI r'AATT_y
Cuts at: 0 814 979 2100
Size: 814 165 1121
Fragments arranged by size:
1121 814 165
AvaI C'yCGr_G
Cuts at: 0 616 1693 1918 2100
Size: 616 1077 225 182
Fragments arranged by size:
1077 616 225 182
BanI G'GyrC_C
Cuts at: 0 1092 2100
Size: 1092 1008
BanII G_rGCy'C
Cuts at: 0 132 617 767 806 1201 1411 1732 2100
Size: 132 485 150 39 395 210 321 368
Fragments arranged by size:
485 395 368 321 210 150 132 39
BbsI GAAGACnn'nnnn_
Cuts at: 0 275 661 1883 1996 2100
Size: 275 386 1222 113 104
Fragments arranged by size:
1222 386 275 113 104
BbvCI CC'TCA_GC
Cuts at: 0 124 2100
Size: 124 1976
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1173 1207 2100
Size: 1173 34 893
Fragments arranged by size:
1173 893 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1121 2100
Size: 1121 979
BglII A'GATC_T
Cuts at: 0 777 2100
Size: 777 1323
Bme1580I G_kGCm'C
Cuts at: 0 132 645 1097 2100
Size: 132 513 452 1003
Fragments arranged by size:
1003 513 452 132
BmrI ACTGGGnnnn_n'
Cuts at: 0 589 1091 1356 1423 2100
Size: 589 502 265 67 677
Fragments arranged by size:
677 589 502 265 67
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1428 2100
Size: 1428 672
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 619 631 1423 1597 1642 2100
Size: 619 12 792 174 45 458
Fragments arranged by size:
792 619 458 174 45 12
Bpu10I CC'TnA_GC
Cuts at: 0 124 1141 2100
Size: 124 1017 959
Fragments arranged by size:
1017 959 124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 588 1213 2100
Size: 588 625 887
Fragments arranged by size:
887 625 588
BsaBI GATnn'nnATC
Cuts at: 0 1171 2100
Size: 1171 929
BsaWI w'CCGG_w
Cuts at: 0 1745 2100
Size: 1745 355
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 670 700 2100
Size: 670 30 1400
Fragments arranged by size:
1400 670 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 124 433 436 724 739 886 1639 2100
Size: 124 309 3 288 15 147 753 461
Fragments arranged by size:
753 461 309 288 147 124 15 3
BseYI C'CCAG_C
Cuts at: 0 348 456 467 644 1180 2068 2100
Size: 348 108 11 177 536 888 32
Fragments arranged by size:
888 536 348 177 108 32 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 22 1054 1450 1455 1471 2100
Size: 22 1032 396 5 16 629
Fragments arranged by size:
1032 629 396 22 16 5
BsiHKAI G_wGCw'C
Cuts at: 0 617 1229 1330 2100
Size: 617 612 101 770
Fragments arranged by size:
770 617 612 101
Bsp1286I G_dGCh'C
Cuts at: 0 132 617 645 767 806 1097 1201 1229
Size: 132 485 28 122 39 291 104 28
Cuts at: 1229 1330 1411 1732 2100
Size: 101 81 321 368
Fragments arranged by size:
485 368 321 291 132 122 104 101
81 39 28 28
BspEI T'CCGG_A
Cuts at: 0 1745 2100
Size: 1745 355
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1974 2100
Size: 1974 126
BsrDI GCAATG_nn'
Cuts at: 0 208 2100
Size: 208 1892
BsrFI r'CCGG_y
Cuts at: 0 344 2100
Size: 344 1756
BstAPI GCAn_nnn'nTGC
Cuts at: 0 437 1065 2100
Size: 437 628 1035
Fragments arranged by size:
1035 628 437
BstEII G'GTnAC_C
Cuts at: 0 1452 2100
Size: 1452 648
BstYI r'GATC_y
Cuts at: 0 24 777 2100
Size: 24 753 1323
Fragments arranged by size:
1323 753 24
Bsu36I CC'TnA_GG
Cuts at: 0 392 992 2100
Size: 392 600 1108
Fragments arranged by size:
1108 600 392
BtgI C'CryG_G
Cuts at: 0 253 650 1532 2100
Size: 253 397 882 568
Fragments arranged by size:
882 568 397 253
BtsI GCAGTG_nn'
Cuts at: 0 319 364 794 1462 1801 1937 2100
Size: 319 45 430 668 339 136 163
Fragments arranged by size:
668 430 339 319 163 136 45
DraIII CAC_nnn'GTG
Cuts at: 0 453 2100
Size: 453 1647
DrdI GACnn_nn'nnGTC
Cuts at: 0 277 2100
Size: 277 1823
EaeI y'GGCC_r
Cuts at: 0 170 1122 2100
Size: 170 952 978
Fragments arranged by size:
978 952 170
EarI CTCTTCn'nnn_
Cuts at: 0 1237 1699 1851 2100
Size: 1237 462 152 249
Fragments arranged by size:
1237 462 249 152
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 24 1074 1740 2100
Size: 24 1050 666 360
Fragments arranged by size:
1050 666 360 24
EcoICRI GAG'CTC
Cuts at: 0 615 2100
Size: 615 1485
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 619 631 947 1208 1423 1597 1615
Size: 358 261 12 316 261 215 174 18
Cuts at: 1615 1642 2083 2100
Size: 27 441 17
Fragments arranged by size:
441 358 316 261 261 215 174 27
18 17 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 244 991 1624 2100
Size: 244 747 633 476
Fragments arranged by size:
747 633 476 244
EcoO109I rG'GnC_Cy
Cuts at: 0 128 129 2100
Size: 128 1 1971
Fragments arranged by size:
1971 128 1
EcoRI G'AATT_C
Cuts at: 0 814 2100
Size: 814 1286
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 985 1017 2100
Size: 985 32 1083
Fragments arranged by size:
1083 985 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 929 2100
Size: 929 1171
MscI TGG'CCA
Cuts at: 0 172 1124 2100
Size: 172 952 976
Fragments arranged by size:
976 952 172
MslI CAynn'nnrTG
Cuts at: 0 1429 2100
Size: 1429 671
MspA1I CmG'CkG
Cuts at: 0 123 467 907 1038 2100
Size: 123 344 440 131 1062
Fragments arranged by size:
1062 440 344 131 123
NaeI GCC'GGC
Cuts at: 0 346 2100
Size: 346 1754
NcoI C'CATG_G
Cuts at: 0 253 1532 2100
Size: 253 1279 568
Fragments arranged by size:
1279 568 253
NgoMIV G'CCGG_C
Cuts at: 0 344 2100
Size: 344 1756
NspI r_CATG'y
Cuts at: 0 1501 2100
Size: 1501 599
PflMI CCAn_nnn'nTGG
Cuts at: 0 1446 1985 2100
Size: 1446 539 115
Fragments arranged by size:
1446 539 115
PfoI T'CCnGG_A
Cuts at: 0 1295 2100
Size: 1295 805
PspOMI G'GGCC_C
Cuts at: 0 128 2100
Size: 128 1972
PspXI vC'TCGA_Gb
Cuts at: 0 616 2100
Size: 616 1484
PstI C_TGCA'G
Cuts at: 0 1008 2100
Size: 1008 1092
PvuII CAG'CTG
Cuts at: 0 123 1038 2100
Size: 123 915 1062
Fragments arranged by size:
1062 915 123
SacI G_AGCT'C
Cuts at: 0 617 2100
Size: 617 1483
ScaI AGT'ACT
Cuts at: 0 1528 2100
Size: 1528 572
SfcI C'TryA_G
Cuts at: 0 1004 2100
Size: 1004 1096
SmlI C'TyrA_G
Cuts at: 0 567 616 1228 2100
Size: 567 49 612 872
Fragments arranged by size:
872 612 567 49
StyI C'CwwG_G
Cuts at: 0 253 806 1532 2100
Size: 253 553 726 568
Fragments arranged by size:
726 568 553 253
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 430 864 1120 2100
Size: 430 434 256 980
Fragments arranged by size:
980 434 430 256
TatI w'GTAC_w
Cuts at: 0 209 599 1526 2100
Size: 209 390 927 574
Fragments arranged by size:
927 574 390 209
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 70 102 1233 1265 2100
Size: 70 32 1131 32 835
Fragments arranged by size:
1131 835 70 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 241 2100
Size: 241 1859
XhoI C'TCGA_G
Cuts at: 0 616 2100
Size: 616 1484
Enzymes that do cut:
AarI AcuI AleI AloI AlwNI ApaI ApoI AvaI
BanI BanII BbsI BbvCI BcgI BglI BglII Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaBI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspMI BsrDI
BsrFI BstAPI BstEII BstYI Bsu36I BtgI BtsI DraIII
DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI Hin4I MmeI MscI MslI MspA1I NaeI NcoI
NgoMIV NspI PflMI PfoI PspOMI PspXI PstI PvuII
SacI ScaI SfcI SmlI StyI TaqII TatI TstI
Tth111I XhoI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BciVI BclI BfrBI BlpI BmgBI BmtI
BsaI BsaAI BsaHI BsiEI BsiWI BsmI BsmBI BspHI
BsrBI BsrGI BssHII BssSI BstBI BstXI BstZ17I BtgZI
ClaI CspCI DraI EagI EcoRV FalI FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI NarI NdeI NheI NotI NruI NsiI PacI
PasI PciI PmeI PmlI PpiI PpuMI PshAI PsiI
PsrI PvuI RsrII SacII SalI SanDI SapI SbfI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI XbaI XcmI XmaI
XmnI ZraI