Restriction map for
pF1KB6190
(Linear) (Six-base) MAPSORT of: KIBB6190.seq Check: 6286 from: 1 to: 1977
KIBB6190.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 417 735 930 1434 1518 1977
Size: 417 318 195 504 84 459
Fragments arranged by size:
504 459 417 318 195 84
AflIII A'CryG_T
Cuts at: 0 352 482 1340 1977
Size: 352 130 858 637
Fragments arranged by size:
858 637 352 130
AleI CACnn'nnGTG
Cuts at: 0 269 1468 1977
Size: 269 1199 509
Fragments arranged by size:
1199 509 269
AlwNI CAG_nnn'CTG
Cuts at: 0 345 1977
Size: 345 1632
ApaLI G'TGCA_C
Cuts at: 0 348 1977
Size: 348 1629
ApoI r'AATT_y
Cuts at: 0 895 1064 1192 1232 1276 1528 1568 1780
Size: 895 169 128 40 44 252 40 212
Cuts at: 1780 1977
Size: 197
Fragments arranged by size:
895 252 212 197 169 128 44 40
40
AseI AT'TA_AT
Cuts at: 0 800 1977
Size: 800 1177
AvaI C'yCGr_G
Cuts at: 0 71 1977
Size: 71 1906
BanI G'GyrC_C
Cuts at: 0 186 1977
Size: 186 1791
BanII G_rGCy'C
Cuts at: 0 1926 1977
Size: 1926 51
BbvCI CC'TCA_GC
Cuts at: 0 737 1977
Size: 737 1240
BfrBI ATG'CAT
Cuts at: 0 599 1262 1977
Size: 599 663 715
Fragments arranged by size:
715 663 599
BglII A'GATC_T
Cuts at: 0 997 1977
Size: 997 980
Bme1580I G_kGCm'C
Cuts at: 0 352 1977
Size: 352 1625
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 606 1010 1178 1248 1430 1514 1598
Size: 151 455 404 168 70 182 84 84
Cuts at: 1598 1614 1766 1850 1934 1977
Size: 16 152 84 84 43
Fragments arranged by size:
455 404 182 168 152 151 84 84
84 84 70 43 16
Bpu10I CC'TnA_GC
Cuts at: 0 737 1977
Size: 737 1240
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 1977
Size: 112 1865
BsaI GGTCTCn'nnnn_
Cuts at: 0 738 1977
Size: 738 1239
BsaAI yAC'GTr
Cuts at: 0 355 503 1341 1845 1977
Size: 355 148 838 504 132
Fragments arranged by size:
838 504 355 148 132
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 91 109 1977
Size: 91 18 1868
Fragments arranged by size:
1868 91 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 525 1197 1365 1449 1701 1785 1795 1977
Size: 525 672 168 84 252 84 10 182
Fragments arranged by size:
672 525 252 182 168 84 84 10
BsiHKAI G_wGCw'C
Cuts at: 0 352 1226 1926 1977
Size: 352 874 700 51
Fragments arranged by size:
874 700 352 51
BsmI GAATG_Cn'
Cuts at: 0 601 1264 1977
Size: 601 663 713
Fragments arranged by size:
713 663 601
Bsp1286I G_dGCh'C
Cuts at: 0 352 1226 1926 1977
Size: 352 874 700 51
Fragments arranged by size:
874 700 352 51
BsrDI GCAATG_nn'
Cuts at: 0 1605 1977
Size: 1605 372
BsrGI T'GTAC_A
Cuts at: 0 472 1557 1977
Size: 472 1085 420
Fragments arranged by size:
1085 472 420
BssSI C'ACGA_G
Cuts at: 0 1927 1977
Size: 1927 50
BstYI r'GATC_y
Cuts at: 0 493 997 1333 1977
Size: 493 504 336 644
Fragments arranged by size:
644 504 493 336
BtsI GCAGTG_nn'
Cuts at: 0 933 1017 1185 1437 1689 1773 1977
Size: 933 84 168 252 252 84 204
Fragments arranged by size:
933 252 252 204 168 84 84
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1083 1118 1587 1622 1977
Size: 1083 35 469 35 355
Fragments arranged by size:
1083 469 355 35 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 240 1977
Size: 240 1737
EarI CTCTTCn'nnn_
Cuts at: 0 460 716 1977
Size: 460 256 1261
Fragments arranged by size:
1261 460 256
EcoICRI GAG'CTC
Cuts at: 0 1924 1977
Size: 1924 53
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 417 606 735 930 1010 1178 1248
Size: 151 266 189 129 195 80 168 70
Cuts at: 1248 1430 1434 1514 1518 1598 1614 1766 1850
Size: 182 4 80 4 80 16 152 84
Cuts at: 1850 1934 1977
Size: 84 43
Fragments arranged by size:
266 195 189 182 168 152 151 129
84 84 80 80 80 70 43 16
4 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 94 1977
Size: 94 1883
EcoO109I rG'GnC_Cy
Cuts at: 0 452 1250 1977
Size: 452 798 727
Fragments arranged by size:
798 727 452
EcoRI G'AATT_C
Cuts at: 0 1064 1232 1568 1977
Size: 1064 168 336 409
Fragments arranged by size:
1064 409 336 168
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1147 1399 1483 1977
Size: 1147 252 84 494
Fragments arranged by size:
1147 494 252 84
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1571 1977
Size: 1571 406
MslI CAynn'nnrTG
Cuts at: 0 267 269 1468 1977
Size: 267 2 1199 509
Fragments arranged by size:
1199 509 267 2
MspA1I CmG'CkG
Cuts at: 0 7 1977
Size: 7 1970
NsiI A_TGCA'T
Cuts at: 0 601 1264 1977
Size: 601 663 713
Fragments arranged by size:
713 663 601
NspI r_CATG'y
Cuts at: 0 486 926 1977
Size: 486 440 1051
Fragments arranged by size:
1051 486 440
PciI A'CATG_T
Cuts at: 0 482 1977
Size: 482 1495
PfoI T'CCnGG_A
Cuts at: 0 695 1977
Size: 695 1282
PmlI CAC'GTG
Cuts at: 0 355 1977
Size: 355 1622
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 40 72 1977
Size: 40 32 1905
Fragments arranged by size:
1905 40 32
PsiI TTA'TAA
Cuts at: 0 1088 1977
Size: 1088 889
PstI C_TGCA'G
Cuts at: 0 1012 1977
Size: 1012 965
SacI G_AGCT'C
Cuts at: 0 1926 1977
Size: 1926 51
SapI GCTCTTCn'nnn_
Cuts at: 0 460 1977
Size: 460 1517
SfcI C'TryA_G
Cuts at: 0 209 1008 1029 1869 1977
Size: 209 799 21 840 108
Fragments arranged by size:
840 799 209 108 21
SmlI C'TyrA_G
Cuts at: 0 91 1977
Size: 91 1886
SnaBI TAC'GTA
Cuts at: 0 1845 1977
Size: 1845 132
StuI AGG'CCT
Cuts at: 0 943 1083 1167 1363 1419 1447 1503 1671
Size: 943 140 84 196 56 28 56 168
Cuts at: 1671 1977
Size: 306
Fragments arranged by size:
943 306 196 168 140 84 56 56
28
StyI C'CwwG_G
Cuts at: 0 639 1977
Size: 639 1338
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 281 1977
Size: 281 1696
TatI w'GTAC_w
Cuts at: 0 62 472 1557 1977
Size: 62 410 1085 420
Fragments arranged by size:
1085 420 410 62
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 92 124 1977
Size: 92 32 1853
Fragments arranged by size:
1853 92 32
XmnI GAAnn'nnTTC
Cuts at: 0 430 691 943 1447 1977
Size: 430 261 252 504 530
Fragments arranged by size:
530 504 430 261 252
Enzymes that do cut:
AcuI AflIII AleI AlwNI ApaLI ApoI AseI AvaI
BanI BanII BbvCI BfrBI BglII Bme1580I BpmI Bpu10I
BpuEI BsaI BsaAI BseRI BsgI BsiHKAI BsmI Bsp1286I
BsrDI BsrGI BssSI BstYI BtsI CspCI DrdI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI MmeI MslI
MspA1I NsiI NspI PciI PfoI PmlI PpiI PsiI
PstI SacI SapI SfcI SmlI SnaBI StuI StyI
TaqII TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI ApaI AscI AsiSI AvrII BaeI
BamHI BbeI BbsI BcgI BciVI BclI BglI BlpI
BmgBI BmrI BmtI BplI BsaBI BsaHI BsaWI BsaXI
BseYI BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI
BsrFI BssHII BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgI BtgZI ClaI DraI DraIII EaeI EagI EciI
EcoRV FseI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI PacI PasI
PflMI PmeI PpuMI PshAI PspOMI PspXI PsrI PvuI
PvuII RsrII SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SpeI SphI SrfI
SspI SwaI Tth111I XbaI XcmI XhoI XmaI ZraI