Restriction map for
pF1KB6202
(Linear) (Six-base) MAPSORT of: KIBB6202.seq Check: 6644 from: 1 to: 2133
KIBB6202.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 213 290 1119 2133
Size: 90 123 77 829 1014
Fragments arranged by size:
1014 829 123 90 77
AleI CACnn'nnGTG
Cuts at: 0 1436 1745 2133
Size: 1436 309 388
Fragments arranged by size:
1436 388 309
AlwNI CAG_nnn'CTG
Cuts at: 0 39 129 1520 2133
Size: 39 90 1391 613
Fragments arranged by size:
1391 613 90 39
ApaLI G'TGCA_C
Cuts at: 0 1764 2133
Size: 1764 369
ApoI r'AATT_y
Cuts at: 0 15 455 812 896 1232 1484 1568 1652
Size: 15 440 357 84 336 252 84 84
Cuts at: 1652 1736 1800 1988 2133
Size: 84 64 188 145
Fragments arranged by size:
440 357 336 252 188 145 84 84
84 84 64 15
BanII G_rGCy'C
Cuts at: 0 176 2133
Size: 176 1957
BfrBI ATG'CAT
Cuts at: 0 1258 1644 2133
Size: 1258 386 489
Fragments arranged by size:
1258 489 386
Bme1580I G_kGCm'C
Cuts at: 0 1768 2133
Size: 1768 365
BmgBI CAC'GTC
Cuts at: 0 93 2133
Size: 93 2040
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1183 2133
Size: 1183 950
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 190 1598 2133
Size: 190 1408 535
Fragments arranged by size:
1408 535 190
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 784 1957 2133
Size: 784 1173 176
Fragments arranged by size:
1173 784 176
BsaBI GATnn'nnATC
Cuts at: 0 1475 2133
Size: 1475 658
BsaWI w'CCGG_w
Cuts at: 0 1660 2133
Size: 1660 473
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 130 277 2133
Size: 130 147 1856
Fragments arranged by size:
1856 147 130
BsiHKAI G_wGCw'C
Cuts at: 0 176 1768 2133
Size: 176 1592 365
Fragments arranged by size:
1592 365 176
BsmI GAATG_Cn'
Cuts at: 0 239 1180 1357 1516 1609 1936 2133
Size: 239 941 177 159 93 327 197
Fragments arranged by size:
941 327 239 197 177 159 93
Bsp1286I G_dGCh'C
Cuts at: 0 176 1768 2133
Size: 176 1592 365
Fragments arranged by size:
1592 365 176
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1784 2133
Size: 1784 349
BsrDI GCAATG_nn'
Cuts at: 0 657 2133
Size: 657 1476
BssSI C'ACGA_G
Cuts at: 0 549 2133
Size: 549 1584
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1604 2133
Size: 1604 529
BstEII G'GTnAC_C
Cuts at: 0 760 2133
Size: 760 1373
BstXI CCAn_nnnn'nTGG
Cuts at: 0 925 1102 1438 2133
Size: 925 177 336 695
Fragments arranged by size:
925 695 336 177
BstYI r'GATC_y
Cuts at: 0 61 2133
Size: 61 2072
Bsu36I CC'TnA_GG
Cuts at: 0 32 2133
Size: 32 2101
BtgI C'CryG_G
Cuts at: 0 98 2133
Size: 98 2035
BtsI GCAGTG_nn'
Cuts at: 0 200 1185 1269 1521 1605 1689 2133
Size: 200 985 84 252 84 84 444
Fragments arranged by size:
985 444 252 200 84 84 84
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 999 1034 2133
Size: 999 35 1099
Fragments arranged by size:
1099 999 35
EaeI y'GGCC_r
Cuts at: 0 783 2133
Size: 783 1350
EarI CTCTTCn'nnn_
Cuts at: 0 193 714 1138 2133
Size: 193 521 424 995
Fragments arranged by size:
995 521 424 193
EcoICRI GAG'CTC
Cuts at: 0 174 2133
Size: 174 1959
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 90 190 213 290 1119 1598 2133
Size: 90 100 23 77 829 479 535
Fragments arranged by size:
829 535 479 100 90 77 23
EcoO109I rG'GnC_Cy
Cuts at: 0 36 528 554 2133
Size: 36 492 26 1579
Fragments arranged by size:
1579 492 36 26
EcoRI G'AATT_C
Cuts at: 0 896 1484 1652 1736 1988 2133
Size: 896 588 168 84 252 145
Fragments arranged by size:
896 588 252 168 145 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1113 1145 1377 1409 1881 1913 2133
Size: 1113 32 232 32 472 32 220
Fragments arranged by size:
1113 472 232 220 32 32 32
HincII GTy'rAC
Cuts at: 0 1295 2133
Size: 1295 838
HindIII A'AGCT_T
Cuts at: 0 341 2133
Size: 341 1792
MfeI C'AATT_G
Cuts at: 0 513 2133
Size: 513 1620
MscI TGG'CCA
Cuts at: 0 785 2133
Size: 785 1348
MslI CAynn'nnrTG
Cuts at: 0 923 989 1100 1241 1436 1745 2133
Size: 923 66 111 141 195 309 388
Fragments arranged by size:
923 388 309 195 141 111 66
NsiI A_TGCA'T
Cuts at: 0 1260 1646 2133
Size: 1260 386 487
Fragments arranged by size:
1260 487 386
NspI r_CATG'y
Cuts at: 0 1262 1644 2133
Size: 1262 382 489
Fragments arranged by size:
1262 489 382
PasI CC'CwG_GG
Cuts at: 0 532 2133
Size: 532 1601
PpuMI rG'GwC_Cy
Cuts at: 0 36 528 2133
Size: 36 492 1605
Fragments arranged by size:
1605 492 36
PstI C_TGCA'G
Cuts at: 0 724 2133
Size: 724 1409
SacI G_AGCT'C
Cuts at: 0 176 2133
Size: 176 1957
SapI GCTCTTCn'nnn_
Cuts at: 0 714 2133
Size: 714 1419
SfcI C'TryA_G
Cuts at: 0 177 720 2133
Size: 177 543 1413
Fragments arranged by size:
1413 543 177
SmlI C'TyrA_G
Cuts at: 0 799 1972 2133
Size: 799 1173 161
Fragments arranged by size:
1173 799 161
SphI G_CATG'C
Cuts at: 0 1262 1644 2133
Size: 1262 382 489
Fragments arranged by size:
1262 489 382
SspI AAT'ATT
Cuts at: 0 732 2133
Size: 732 1401
StuI AGG'CCT
Cuts at: 0 1363 1783 1839 2035 2133
Size: 1363 420 56 196 98
Fragments arranged by size:
1363 420 196 98 56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 880 1552 2133
Size: 880 672 581
Fragments arranged by size:
880 672 581
XmnI GAAnn'nnTTC
Cuts at: 0 1447 1615 1699 1783 1951 2133
Size: 1447 168 84 84 168 182
Fragments arranged by size:
1447 182 168 168 84 84
Enzymes that do cut:
AcuI AleI AlwNI ApaLI ApoI BanII BfrBI Bme1580I
BmgBI BplI BpmI BpuEI BsaBI BsaWI BseRI BsiHKAI
BsmI Bsp1286I BspMI BsrDI BssSI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtsI CspCI EaeI EarI EcoICRI
Eco57MI EcoO109I EcoRI Hin4I HincII HindIII MfeI MscI
MslI NsiI NspI PasI PpuMI PstI SacI SapI
SfcI SmlI SphI SspI StuI TaqII XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI BmrI BmtI
Bpu10I BsaI BsaAI BsaHI BsaXI BseYI BsgI BsiEI
BsiWI BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssHII
BstBI BstZ17I BtgZI ClaI DraI DraIII DrdI EagI
EciI EcoNI EcoRV FalI FseI FspI FspAI HaeII
HpaI KasI KpnI MluI MmeI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI PacI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI StyI SwaI TatI TstI Tth111I
XbaI XcmI XhoI XmaI ZraI