Restriction map for
pF1KB6205
(Linear) (Six-base) MAPSORT of: KIBB6205.seq Check: 3965 from: 1 to: 1992
KIBB6205.orf
With 182 enzymes: SgfI *
October 7, 2009 14:19 ..
AccI GT'mk_AC
Cuts at: 0 1308 1992
Size: 1308 684
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 520 1334 1550 1992
Size: 520 814 216 442
Fragments arranged by size:
814 520 442 216
AlwNI CAG_nnn'CTG
Cuts at: 0 796 972 1248 1992
Size: 796 176 276 744
Fragments arranged by size:
796 744 276 176
ApoI r'AATT_y
Cuts at: 0 1254 1378 1992
Size: 1254 124 614
Fragments arranged by size:
1254 614 124
AseI AT'TA_AT
Cuts at: 0 1826 1882 1992
Size: 1826 56 110
Fragments arranged by size:
1826 110 56
AvaI C'yCGr_G
Cuts at: 0 1568 1992
Size: 1568 424
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1640 1673 1992
Size: 1640 33 319
Fragments arranged by size:
1640 319 33
BamHI G'GATC_C
Cuts at: 0 1860 1992
Size: 1860 132
BanI G'GyrC_C
Cuts at: 0 91 1992
Size: 91 1901
BanII G_rGCy'C
Cuts at: 0 150 596 1992
Size: 150 446 1396
Fragments arranged by size:
1396 446 150
BbsI GAAGACnn'nnnn_
Cuts at: 0 484 1013 1992
Size: 484 529 979
Fragments arranged by size:
979 529 484
BclI T'GATC_A
Cuts at: 0 1386 1992
Size: 1386 606
BglII A'GATC_T
Cuts at: 0 1127 1992
Size: 1127 865
Bme1580I G_kGCm'C
Cuts at: 0 552 1992
Size: 552 1440
BmtI G_CTAG'C
Cuts at: 0 410 1992
Size: 410 1582
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 773 974 1048 1104 1778 1992
Size: 106 667 201 74 56 674 214
Fragments arranged by size:
674 667 214 201 106 74 56
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1637 1992
Size: 1637 355
BsaBI GATnn'nnATC
Cuts at: 0 1955 1992
Size: 1955 37
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 514 544 1102 1132 1392 1422 1992
Size: 514 30 558 30 260 30 570
Fragments arranged by size:
570 558 514 260 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 43 519 1336 1992
Size: 43 476 817 656
Fragments arranged by size:
817 656 476 43
BseYI C'CCAG_C
Cuts at: 0 749 1237 1992
Size: 749 488 755
Fragments arranged by size:
755 749 488
BsmI GAATG_Cn'
Cuts at: 0 704 1992
Size: 704 1288
Bsp1286I G_dGCh'C
Cuts at: 0 150 552 596 1992
Size: 150 402 44 1396
Fragments arranged by size:
1396 402 150 44
BsrBI CCG'CTC
Cuts at: 0 1180 1992
Size: 1180 812
BsrDI GCAATG_nn'
Cuts at: 0 134 417 1992
Size: 134 283 1575
Fragments arranged by size:
1575 283 134
BsrFI r'CCGG_y
Cuts at: 0 1010 1992
Size: 1010 982
BstEII G'GTnAC_C
Cuts at: 0 844 876 1614 1992
Size: 844 32 738 378
Fragments arranged by size:
844 738 378 32
BstXI CCAn_nnnn'nTGG
Cuts at: 0 276 663 841 1992
Size: 276 387 178 1151
Fragments arranged by size:
1151 387 276 178
BstYI r'GATC_y
Cuts at: 0 363 390 1127 1641 1860 1992
Size: 363 27 737 514 219 132
Fragments arranged by size:
737 514 363 219 132 27
Bsu36I CC'TnA_GG
Cuts at: 0 1148 1992
Size: 1148 844
BtgI C'CryG_G
Cuts at: 0 8 503 612 947 995 1992
Size: 8 495 109 335 48 997
Fragments arranged by size:
997 495 335 109 48 8
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1561 1992
Size: 1561 431
BtsI GCAGTG_nn'
Cuts at: 0 1196 1992
Size: 1196 796
DraI TTT'AAA
Cuts at: 0 386 1992
Size: 386 1606
EaeI y'GGCC_r
Cuts at: 0 167 500 581 803 992 1992
Size: 167 333 81 222 189 1000
Fragments arranged by size:
1000 333 222 189 167 81
EarI CTCTTCn'nnn_
Cuts at: 0 1085 1992
Size: 1085 907
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 528 745 1992
Size: 528 217 1247
Fragments arranged by size:
1247 528 217
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 520 773 974 1048 1104 1334 1550
Size: 106 414 253 201 74 56 230 216
Cuts at: 1550 1778 1992
Size: 228 214
Fragments arranged by size:
414 253 230 228 216 214 201 106
74 56
EcoNI CCTnn'n_nnAGG
Cuts at: 0 25 891 1992
Size: 25 866 1101
Fragments arranged by size:
1101 866 25
EcoO109I rG'GnC_Cy
Cuts at: 0 969 1992
Size: 969 1023
EcoRI G'AATT_C
Cuts at: 0 1378 1992
Size: 1378 614
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 778 1480 1992
Size: 778 702 512
Fragments arranged by size:
778 702 512
HaeII r_GCGC'y
Cuts at: 0 1017 1992
Size: 1017 975
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 947 967 979 999 1423 1455 1992
Size: 947 20 12 20 424 32 537
Fragments arranged by size:
947 537 424 32 20 20 12
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 468 1992
Size: 468 1524
MscI TGG'CCA
Cuts at: 0 169 583 1992
Size: 169 414 1409
Fragments arranged by size:
1409 414 169
MspA1I CmG'CkG
Cuts at: 0 117 505 1992
Size: 117 388 1487
Fragments arranged by size:
1487 388 117
NcoI C'CATG_G
Cuts at: 0 612 1992
Size: 612 1380
NdeI CA'TA_TG
Cuts at: 0 201 1992
Size: 201 1791
NheI G'CTAG_C
Cuts at: 0 406 1992
Size: 406 1586
NruI TCG'CGA
Cuts at: 0 1159 1992
Size: 1159 833
PasI CC'CwG_GG
Cuts at: 0 938 1992
Size: 938 1054
PflMI CCAn_nnn'nTGG
Cuts at: 0 887 972 1611 1992
Size: 887 85 639 381
Fragments arranged by size:
887 639 381 85
PfoI T'CCnGG_A
Cuts at: 0 965 1992
Size: 965 1027
PpuMI rG'GwC_Cy
Cuts at: 0 969 1992
Size: 969 1023
PshAI GACnn'nnGTC
Cuts at: 0 484 1992
Size: 484 1508
PstI C_TGCA'G
Cuts at: 0 566 1992
Size: 566 1426
PvuII CAG'CTG
Cuts at: 0 117 1992
Size: 117 1875
SacII CC_GC'GG
Cuts at: 0 506 1992
Size: 506 1486
ScaI AGT'ACT
Cuts at: 0 541 1992
Size: 541 1451
SfcI C'TryA_G
Cuts at: 0 562 1373 1992
Size: 562 811 619
Fragments arranged by size:
811 619 562
SmlI C'TyrA_G
Cuts at: 0 1652 1992
Size: 1652 340
StyI C'CwwG_G
Cuts at: 0 612 912 1992
Size: 612 300 1080
Fragments arranged by size:
1080 612 300
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 496 925 1153 1992
Size: 496 429 228 839
Fragments arranged by size:
839 496 429 228
TatI w'GTAC_w
Cuts at: 0 539 566 1774 1920 1992
Size: 539 27 1208 146 72
Fragments arranged by size:
1208 539 146 72 27
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 250 282 482 514 546 1992
Size: 250 32 200 32 32 1446
Fragments arranged by size:
1446 250 200 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 945 1992
Size: 945 1047
XmnI GAAnn'nnTTC
Cuts at: 0 1442 1992
Size: 1442 550
Enzymes that do cut:
AccI AcuI AlwNI ApoI AseI AvaI BaeI BamHI
BanI BanII BbsI BclI BglII Bme1580I BmtI BpmI
BpuEI BsaBI BsaXI BseRI BseYI BsmI Bsp1286I BsrBI
BsrDI BsrFI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI EaeI EarI EciI Eco57MI EcoNI EcoO109I
EcoRI FalI HaeII Hin4I MmeI MscI MspA1I NcoI
NdeI NheI NruI PasI PflMI PfoI PpuMI PshAI
PstI PvuII SacII ScaI SfcI SmlI StyI TaqII
TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AsiSI
AvrII BbeI BbvCI BcgI BciVI BfrBI BglI BlpI
BmgBI BmrI BplI Bpu10I BsaI BsaAI BsaHI BsaWI
BsgI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI BspMI
BsrGI BssHII BssSI BstAPI BstBI BstZ17I ClaI CspCI
DraIII DrdI EagI EcoICRI EcoRV FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI MslI
NaeI NarI NgoMIV NotI NsiI NspI PacI PciI
PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SalI SanDI SapI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI Tth111I XbaI XhoI XmaI ZraI