Restriction map for
pF1KB6208
(Linear) (Six-base) MAPSORT of: KIBB6208.seq Check: 7901 from: 1 to: 2160
KIBB6208.orf
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AatII G_ACGT'C
Cuts at: 0 128 2160
Size: 128 2032
Acc65I G'GTAC_C
Cuts at: 0 1055 2160
Size: 1055 1105
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 751 808 2160
Size: 751 57 1352
Fragments arranged by size:
1352 751 57
AfeI AGC'GCT
Cuts at: 0 214 2160
Size: 214 1946
AflII C'TTAA_G
Cuts at: 0 709 2160
Size: 709 1451
AflIII A'CryG_T
Cuts at: 0 356 2160
Size: 356 1804
AgeI A'CCGG_T
Cuts at: 0 1058 2160
Size: 1058 1102
AleI CACnn'nnGTG
Cuts at: 0 1945 2160
Size: 1945 215
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 660 2160
Size: 660 1500
AlwNI CAG_nnn'CTG
Cuts at: 0 493 973 2160
Size: 493 480 1187
Fragments arranged by size:
1187 493 480
ApaI G_GGCC'C
Cuts at: 0 532 2160
Size: 532 1628
ApaLI G'TGCA_C
Cuts at: 0 1120 2160
Size: 1120 1040
ApoI r'AATT_y
Cuts at: 0 731 2160
Size: 731 1429
AvaI C'yCGr_G
Cuts at: 0 133 143 173 2160
Size: 133 10 30 1987
Fragments arranged by size:
1987 133 30 10
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1047 1080 2160
Size: 1047 33 1080
Fragments arranged by size:
1080 1047 33
BanI G'GyrC_C
Cuts at: 0 892 1055 1255 1297 1380 1619 1784 1896
Size: 892 163 200 42 83 239 165 112
Cuts at: 1896 2061 2122 2155 2160
Size: 165 61 33 5
Fragments arranged by size:
892 239 200 165 165 163 112 83
61 42 33 5
BanII G_rGCy'C
Cuts at: 0 532 703 981 1015 1444 1704 1940 2160
Size: 532 171 278 34 429 260 236 220
Fragments arranged by size:
532 429 278 260 236 220 171 34
BbeI G_GCGC'C
Cuts at: 0 1623 1788 2126 2160
Size: 1623 165 338 34
Fragments arranged by size:
1623 338 165 34
BclI T'GATC_A
Cuts at: 0 939 2160
Size: 939 1221
BglI GCCn_nnn'nGGC
Cuts at: 0 1019 2160
Size: 1019 1141
BlpI GC'TnA_GC
Cuts at: 0 1961 2160
Size: 1961 199
Bme1580I G_kGCm'C
Cuts at: 0 532 895 949 1124 1302 1495 1660 2158
Size: 532 363 54 175 178 193 165 498
Cuts at: 2158 2160
Size: 2
Fragments arranged by size:
532 498 363 193 178 175 165 54
2
BmgBI CAC'GTC
Cuts at: 0 357 780 2160
Size: 357 423 1380
Fragments arranged by size:
1380 423 357
BmrI ACTGGGnnnn_n'
Cuts at: 0 430 657 929 1740 2160
Size: 430 227 272 811 420
Fragments arranged by size:
811 430 420 272 227
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 169 303 2016 2160
Size: 169 134 1713 144
Fragments arranged by size:
1713 169 144 134
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 433 995 2160
Size: 433 562 1165
Fragments arranged by size:
1165 562 433
BsaI GGTCTCn'nnnn_
Cuts at: 0 1259 1676 2160
Size: 1259 417 484
Fragments arranged by size:
1259 484 417
BsaHI Gr'CG_yC
Cuts at: 0 125 1620 1785 2123 2160
Size: 125 1495 165 338 37
Fragments arranged by size:
1495 338 165 125 37
BsaWI w'CCGG_w
Cuts at: 0 94 1058 1720 2160
Size: 94 964 662 440
Fragments arranged by size:
964 662 440 94
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 319 349 1426 1456 1679 1709 2160
Size: 319 30 1077 30 223 30 451
Fragments arranged by size:
1077 451 319 223 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 28 292 340 1192 2005 2083 2160
Size: 19 9 264 48 852 813 78 77
Fragments arranged by size:
852 813 264 78 77 48 19 9
BseYI C'CCAG_C
Cuts at: 0 512 542 842 896 980 1023 1200 1259
Size: 512 30 300 54 84 43 177 59
Cuts at: 1259 1601 2086 2160
Size: 342 485 74
Fragments arranged by size:
512 485 342 300 177 84 74 59
54 43 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1699 2160
Size: 1699 461
BsiHKAI G_wGCw'C
Cuts at: 0 1124 1280 1704 1952 2160
Size: 1124 156 424 248 208
Fragments arranged by size:
1124 424 248 208 156
BsmI GAATG_Cn'
Cuts at: 0 796 1081 2160
Size: 796 285 1079
Fragments arranged by size:
1079 796 285
BsmBI CGTCTCn'nnnn_
Cuts at: 0 347 2160
Size: 347 1813
Bsp1286I G_dGCh'C
Cuts at: 0 532 703 895 949 981 1015 1124 1280
Size: 532 171 192 54 32 34 109 156
Cuts at: 1280 1302 1444 1495 1660 1704 1940 1952 2158
Size: 22 142 51 165 44 236 12 206
Cuts at: 2158 2160
Size: 2
Fragments arranged by size:
532 236 206 192 171 165 156 142
109 54 51 44 34 32 22 12
2
BspEI T'CCGG_A
Cuts at: 0 94 1720 2160
Size: 94 1626 440
Fragments arranged by size:
1626 440 94
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1538 2160
Size: 1538 622
BsrDI GCAATG_nn'
Cuts at: 0 760 2160
Size: 760 1400
BsrFI r'CCGG_y
Cuts at: 0 1058 1343 1886 2160
Size: 1058 285 543 274
Fragments arranged by size:
1058 543 285 274
BsrGI T'GTAC_A
Cuts at: 0 600 2160
Size: 600 1560
BssSI C'ACGA_G
Cuts at: 0 1705 2160
Size: 1705 455
BstEII G'GTnAC_C
Cuts at: 0 2025 2160
Size: 2025 135
BstXI CCAn_nnnn'nTGG
Cuts at: 0 754 904 988 2160
Size: 754 150 84 1172
Fragments arranged by size:
1172 754 150 84
BstYI r'GATC_y
Cuts at: 0 651 2160
Size: 651 1509
Bsu36I CC'TnA_GG
Cuts at: 0 1864 1892 2160
Size: 1864 28 268
Fragments arranged by size:
1864 268 28
BtgI C'CryG_G
Cuts at: 0 316 346 1209 2160
Size: 316 30 863 951
Fragments arranged by size:
951 863 316 30
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1348 2160
Size: 1348 812
BtsI GCAGTG_nn'
Cuts at: 0 581 867 2160
Size: 581 286 1293
Fragments arranged by size:
1293 581 286
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1515 1550 2160
Size: 1515 35 610
Fragments arranged by size:
1515 610 35
DraI TTT'AAA
Cuts at: 0 161 2160
Size: 161 1999
DraIII CAC_nnn'GTG
Cuts at: 0 1154 1284 1380 1585 2160
Size: 1154 130 96 205 575
Fragments arranged by size:
1154 575 205 130 96
EaeI y'GGCC_r
Cuts at: 0 914 1430 1503 1752 1769 2160
Size: 914 516 73 249 17 391
Fragments arranged by size:
914 516 391 249 73 17
EarI CTCTTCn'nnn_
Cuts at: 0 269 2160
Size: 269 1891
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 25 2160
Size: 25 2135
EcoICRI GAG'CTC
Cuts at: 0 1702 2160
Size: 1702 458
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 433 751 808 995 2160
Size: 433 318 57 187 1165
Fragments arranged by size:
1165 433 318 187 57
EcoO109I rG'GnC_Cy
Cuts at: 0 114 237 528 849 1674 1730 2160
Size: 114 123 291 321 825 56 430
Fragments arranged by size:
825 430 321 291 123 114 56
HaeII r_GCGC'y
Cuts at: 0 43 216 1623 1713 1788 2126 2160
Size: 43 173 1407 90 75 338 34
Fragments arranged by size:
1407 338 173 90 75 43 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 319 351 444 476 2160
Size: 319 32 93 32 1684
Fragments arranged by size:
1684 319 93 32 32
KasI G'GCGC_C
Cuts at: 0 1619 1784 2122 2160
Size: 1619 165 338 38
Fragments arranged by size:
1619 338 165 38
KpnI G_GTAC'C
Cuts at: 0 1059 2160
Size: 1059 1101
MscI TGG'CCA
Cuts at: 0 916 1432 1771 2160
Size: 916 516 339 389
Fragments arranged by size:
916 516 389 339
MslI CAynn'nnrTG
Cuts at: 0 1295 1945 2160
Size: 1295 650 215
Fragments arranged by size:
1295 650 215
MspA1I CmG'CkG
Cuts at: 0 270 477 568 1092 1387 1521 1574 1605
Size: 270 207 91 524 295 134 53 31
Cuts at: 1605 1823 2160
Size: 218 337
Fragments arranged by size:
524 337 295 270 218 207 134 91
53 31
NaeI GCC'GGC
Cuts at: 0 1345 2160
Size: 1345 815
NarI GG'CG_CC
Cuts at: 0 1620 1785 2123 2160
Size: 1620 165 338 37
Fragments arranged by size:
1620 338 165 37
NcoI C'CATG_G
Cuts at: 0 346 2160
Size: 346 1814
NdeI CA'TA_TG
Cuts at: 0 1196 1457 2160
Size: 1196 261 703
Fragments arranged by size:
1196 703 261
NgoMIV G'CCGG_C
Cuts at: 0 1343 2160
Size: 1343 817
PasI CC'CwG_GG
Cuts at: 0 622 2160
Size: 622 1538
PflMI CCAn_nnn'nTGG
Cuts at: 0 973 1200 2160
Size: 973 227 960
Fragments arranged by size:
973 960 227
PpuMI rG'GwC_Cy
Cuts at: 0 237 849 2160
Size: 237 612 1311
Fragments arranged by size:
1311 612 237
PspOMI G'GGCC_C
Cuts at: 0 528 2160
Size: 528 1632
PstI C_TGCA'G
Cuts at: 0 248 1906 1958 2160
Size: 248 1658 52 202
Fragments arranged by size:
1658 248 202 52
PvuII CAG'CTG
Cuts at: 0 477 568 1092 1387 1605 2160
Size: 477 91 524 295 218 555
Fragments arranged by size:
555 524 477 295 218 91
SacI G_AGCT'C
Cuts at: 0 1704 2160
Size: 1704 456
SanDI GG'GwC_CC
Cuts at: 0 237 2160
Size: 237 1923
SbfI CC_TGCA'GG
Cuts at: 0 1958 2160
Size: 1958 202
SexAI A'CCwGG_T
Cuts at: 0 1280 1376 2160
Size: 1280 96 784
Fragments arranged by size:
1280 784 96
SfcI C'TryA_G
Cuts at: 0 244 569 1087 1167 1902 1954 2160
Size: 244 325 518 80 735 52 206
Fragments arranged by size:
735 518 325 244 206 80 52
SfoI GGC'GCC
Cuts at: 0 1621 1786 2124 2160
Size: 1621 165 338 36
Fragments arranged by size:
1621 338 165 36
SmaI CCC'GGG
Cuts at: 0 175 2160
Size: 175 1985
SmlI C'TyrA_G
Cuts at: 0 709 2160
Size: 709 1451
StuI AGG'CCT
Cuts at: 0 1249 1333 2160
Size: 1249 84 827
Fragments arranged by size:
1249 827 84
StyI C'CwwG_G
Cuts at: 0 186 203 290 346 737 2160
Size: 186 17 87 56 391 1423
Fragments arranged by size:
1423 391 186 87 56 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 556 743 910 966 1273 2160
Size: 556 187 167 56 307 887
Fragments arranged by size:
887 556 307 187 167 56
TatI w'GTAC_w
Cuts at: 0 600 2160
Size: 600 1560
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1679 1711 2160
Size: 1679 32 449
Fragments arranged by size:
1679 449 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 670 1836 2160
Size: 670 1166 324
Fragments arranged by size:
1166 670 324
XmaI C'CCGG_G
Cuts at: 0 173 2160
Size: 173 1987
ZraI GAC'GTC
Cuts at: 0 126 2160
Size: 126 2034
Enzymes that do cut:
AatII Acc65I AcuI AfeI AflII AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AvaI BaeI BanI
BanII BbeI BclI BglI BlpI Bme1580I BmgBI BmrI
BplI BpmI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrDI
BsrFI BsrGI BssSI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraI DraIII EaeI EarI EciI
EcoICRI Eco57MI EcoO109I HaeII Hin4I KasI KpnI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV PasI
PflMI PpuMI PspOMI PstI PvuII SacI SanDI SbfI
SexAI SfcI SfoI SmaI SmlI StuI StyI TaqII
TatI TstI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AccI AclI AhdI AloI AscI AseI AsiSI
AvrII BamHI BbsI BbvCI BcgI BciVI BfrBI BglII
BmtI Bpu10I BpuEI BsaAI BsaBI BsiEI BsiWI BspHI
BsrBI BssHII BstAPI BstBI BstZ17I ClaI DrdI EagI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI MfeI MluI MmeI NheI NotI NruI
NsiI NspI PacI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
SapI ScaI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI Tth111I XbaI XhoI XmnI