Restriction map for
pF1KB6213
(Linear) (Six-base) MAPSORT of: KIBB6213.seq Check: 7425 from: 1 to: 2004
KIBB6213.orf
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AccI GT'mk_AC
Cuts at: 0 748 1001 2004
Size: 748 253 1003
Fragments arranged by size:
1003 748 253
Acc65I G'GTAC_C
Cuts at: 0 756 1609 2004
Size: 756 853 395
Fragments arranged by size:
853 756 395
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 995 1231 1307 1351 1724 1927 2004
Size: 268 727 236 76 44 373 203 77
Fragments arranged by size:
727 373 268 236 203 77 76 44
AflIII A'CryG_T
Cuts at: 0 11 923 2004
Size: 11 912 1081
Fragments arranged by size:
1081 912 11
AgeI A'CCGG_T
Cuts at: 0 941 2004
Size: 941 1063
AleI CACnn'nnGTG
Cuts at: 0 659 2004
Size: 659 1345
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 677 2004
Size: 677 1327
AlwNI CAG_nnn'CTG
Cuts at: 0 360 670 2004
Size: 360 310 1334
Fragments arranged by size:
1334 360 310
ApaI G_GGCC'C
Cuts at: 0 1147 1845 2004
Size: 1147 698 159
Fragments arranged by size:
1147 698 159
AvaI C'yCGr_G
Cuts at: 0 590 677 1241 1604 2004
Size: 590 87 564 363 400
Fragments arranged by size:
590 564 400 363 87
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 740 773 2004
Size: 740 33 1231
Fragments arranged by size:
1231 740 33
BanI G'GyrC_C
Cuts at: 0 756 1246 1581 1609 2004
Size: 756 490 335 28 395
Fragments arranged by size:
756 490 395 335 28
BanII G_rGCy'C
Cuts at: 0 89 618 1147 1845 2004
Size: 89 529 529 698 159
Fragments arranged by size:
698 529 529 159 89
BbsI GAAGACnn'nnnn_
Cuts at: 0 418 1219 2004
Size: 418 801 785
Fragments arranged by size:
801 785 418
BciVI GTATCCnnnnn_n'
Cuts at: 0 699 1827 2004
Size: 699 1128 177
Fragments arranged by size:
1128 699 177
BclI T'GATC_A
Cuts at: 0 426 2004
Size: 426 1578
BglI GCCn_nnn'nGGC
Cuts at: 0 814 1327 2004
Size: 814 513 677
Fragments arranged by size:
814 677 513
BglII A'GATC_T
Cuts at: 0 1566 2004
Size: 1566 438
Bme1580I G_kGCm'C
Cuts at: 0 1147 1249 1845 2004
Size: 1147 102 596 159
Fragments arranged by size:
1147 596 159 102
BmrI ACTGGGnnnn_n'
Cuts at: 0 1666 2004
Size: 1666 338
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1278 2004
Size: 1278 726
Bpu10I CC'TnA_GC
Cuts at: 0 737 2004
Size: 737 1267
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 369 1248 1249 2004
Size: 369 879 1 755
Fragments arranged by size:
879 755 369 1
BsaWI w'CCGG_w
Cuts at: 0 941 2004
Size: 941 1063
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 17 47 758 787 788 817 2004
Size: 17 30 711 29 1 29 1187
Fragments arranged by size:
1187 711 30 29 29 17 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 97 844 2004
Size: 97 747 1160
Fragments arranged by size:
1160 747 97
BseYI C'CCAG_C
Cuts at: 0 1791 2004
Size: 1791 213
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 683 1425 1940 2004
Size: 427 256 742 515 64
Fragments arranged by size:
742 515 427 256 64
BsiHKAI G_wGCw'C
Cuts at: 0 89 1486 2004
Size: 89 1397 518
Fragments arranged by size:
1397 518 89
Bsp1286I G_dGCh'C
Cuts at: 0 89 618 1147 1249 1486 1845 2004
Size: 89 529 529 102 237 359 159
Fragments arranged by size:
529 529 359 237 159 102 89
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1962 2004
Size: 1962 42
BsrFI r'CCGG_y
Cuts at: 0 560 941 2004
Size: 560 381 1063
Fragments arranged by size:
1063 560 381
BstAPI GCAn_nnn'nTGC
Cuts at: 0 670 2004
Size: 670 1334
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1338 1812 2004
Size: 1338 474 192
Fragments arranged by size:
1338 474 192
BstYI r'GATC_y
Cuts at: 0 467 1184 1566 2004
Size: 467 717 382 438
Fragments arranged by size:
717 467 438 382
Bsu36I CC'TnA_GG
Cuts at: 0 1499 2004
Size: 1499 505
BtgI C'CryG_G
Cuts at: 0 701 1250 2004
Size: 701 549 754
Fragments arranged by size:
754 701 549
BtsI GCAGTG_nn'
Cuts at: 0 324 1165 1937 2004
Size: 324 841 772 67
Fragments arranged by size:
841 772 324 67
EaeI y'GGCC_r
Cuts at: 0 5 1823 2004
Size: 5 1818 181
Fragments arranged by size:
1818 181 5
EarI CTCTTCn'nnn_
Cuts at: 0 54 194 2004
Size: 54 140 1810
Fragments arranged by size:
1810 140 54
EcoICRI GAG'CTC
Cuts at: 0 87 2004
Size: 87 1917
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 995 1231 1307 1351 1724 1927 2004
Size: 268 727 236 76 44 373 203 77
Fragments arranged by size:
727 373 268 236 203 77 76 44
EcoNI CCTnn'n_nnAGG
Cuts at: 0 364 484 714 736 1849 2004
Size: 364 120 230 22 1113 155
Fragments arranged by size:
1113 364 230 155 120 22
EcoO109I rG'GnC_Cy
Cuts at: 0 537 834 1089 1143 1410 1841 1842 2004
Size: 537 297 255 54 267 431 1 162
Fragments arranged by size:
537 431 297 267 255 162 54 1
EcoRV GAT'ATC
Cuts at: 0 345 684 2004
Size: 345 339 1320
Fragments arranged by size:
1320 345 339
HaeII r_GCGC'y
Cuts at: 0 240 1322 2004
Size: 240 1082 682
Fragments arranged by size:
1082 682 240
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 17 49 459 478 491 510 787 819
Size: 17 32 410 19 13 19 277 32
Cuts at: 819 1206 1238 1515 1547 2004
Size: 387 32 277 32 457
Fragments arranged by size:
457 410 387 277 277 32 32 32
32 19 19 17 13
HincII GTy'rAC
Cuts at: 0 749 2004
Size: 749 1255
HindIII A'AGCT_T
Cuts at: 0 648 2004
Size: 648 1356
KpnI G_GTAC'C
Cuts at: 0 760 1613 2004
Size: 760 853 391
Fragments arranged by size:
853 760 391
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1162 2004
Size: 1162 842
MscI TGG'CCA
Cuts at: 0 1825 2004
Size: 1825 179
MslI CAynn'nnrTG
Cuts at: 0 659 1045 1669 2004
Size: 659 386 624 335
Fragments arranged by size:
659 624 386 335
MspA1I CmG'CkG
Cuts at: 0 667 1638 1795 2004
Size: 667 971 157 209
Fragments arranged by size:
971 667 209 157
NcoI C'CATG_G
Cuts at: 0 701 2004
Size: 701 1303
NspI r_CATG'y
Cuts at: 0 15 927 1158 1404 2004
Size: 15 912 231 246 600
Fragments arranged by size:
912 600 246 231 15
PasI CC'CwG_GG
Cuts at: 0 762 2004
Size: 762 1242
PciI A'CATG_T
Cuts at: 0 11 923 2004
Size: 11 912 1081
Fragments arranged by size:
1081 912 11
PflMI CCAn_nnn'nTGG
Cuts at: 0 360 2004
Size: 360 1644
PfoI T'CCnGG_A
Cuts at: 0 1994 2004
Size: 1994 10
PpuMI rG'GwC_Cy
Cuts at: 0 537 1410 2004
Size: 537 873 594
Fragments arranged by size:
873 594 537
PspOMI G'GGCC_C
Cuts at: 0 1143 1841 2004
Size: 1143 698 163
Fragments arranged by size:
1143 698 163
PvuII CAG'CTG
Cuts at: 0 667 1638 1795 2004
Size: 667 971 157 209
Fragments arranged by size:
971 667 209 157
SacI G_AGCT'C
Cuts at: 0 89 2004
Size: 89 1915
SalI G'TCGA_C
Cuts at: 0 747 2004
Size: 747 1257
SanDI GG'GwC_CC
Cuts at: 0 537 2004
Size: 537 1467
SexAI A'CCwGG_T
Cuts at: 0 65 2004
Size: 65 1939
SmlI C'TyrA_G
Cuts at: 0 348 1228 1263 2004
Size: 348 880 35 741
Fragments arranged by size:
880 741 348 35
StuI AGG'CCT
Cuts at: 0 1731 2004
Size: 1731 273
StyI C'CwwG_G
Cuts at: 0 701 1423 2004
Size: 701 722 581
Fragments arranged by size:
722 701 581
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 313 2004
Size: 313 1691
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 303 335 758 790 2004
Size: 303 32 423 32 1214
Fragments arranged by size:
1214 423 303 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 750 2004
Size: 750 1254
XmnI GAAnn'nnTTC
Cuts at: 0 263 856 2004
Size: 263 593 1148
Fragments arranged by size:
1148 593 263
Enzymes that do cut:
AccI Acc65I AcuI AflIII AgeI AleI AlfI AlwNI
ApaI AvaI BaeI BanI BanII BbsI BciVI BclI
BglI BglII Bme1580I BmrI BplI Bpu10I BpuEI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrFI
BstAPI BstXI BstYI Bsu36I BtgI BtsI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRV HaeII Hin4I HincII
HindIII KpnI MmeI MscI MslI MspA1I NcoI NspI
PasI PciI PflMI PfoI PpuMI PspOMI PvuII SacI
SalI SanDI SexAI SmlI StuI StyI TaqII TstI
XcmI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AhdI AloI ApaLI
ApoI AscI AseI AsiSI AvrII BamHI BbeI BbvCI
BcgI BfrBI BlpI BmgBI BmtI BpmI BsaI BsaAI
BsaBI BsaHI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrDI BsrGI BssHII BssSI BstBI BstEII BstZ17I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoRI FalI FseI FspI FspAI HpaI KasI MfeI
MluI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PstI PvuI RsrII SacII SapI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TatI Tth111I XbaI XhoI
XmaI ZraI