Restriction map for
pF1KB6219
(Linear) (Six-base) MAPSORT of: KIBB6219.seq Check: 6618 from: 1 to: 2007
KIBB6219.orf
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 609 2007
Size: 609 1398
AccI GT'mk_AC
Cuts at: 0 28 2007
Size: 28 1979
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 406 1259 2007
Size: 289 117 853 748
Fragments arranged by size:
853 748 289 117
AleI CACnn'nnGTG
Cuts at: 0 404 964 2007
Size: 404 560 1043
Fragments arranged by size:
1043 560 404
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 733 765 2007
Size: 733 32 1242
Fragments arranged by size:
1242 733 32
AlwNI CAG_nnn'CTG
Cuts at: 0 450 654 2007
Size: 450 204 1353
Fragments arranged by size:
1353 450 204
ApaI G_GGCC'C
Cuts at: 0 985 1921 1972 2007
Size: 985 936 51 35
Fragments arranged by size:
985 936 51 35
ApaLI G'TGCA_C
Cuts at: 0 1375 2007
Size: 1375 632
AvaI C'yCGr_G
Cuts at: 0 56 1963 2007
Size: 56 1907 44
Fragments arranged by size:
1907 56 44
BamHI G'GATC_C
Cuts at: 0 1160 2007
Size: 1160 847
BanI G'GyrC_C
Cuts at: 0 1099 1313 1363 2007
Size: 1099 214 50 644
Fragments arranged by size:
1099 644 214 50
BanII G_rGCy'C
Cuts at: 0 371 628 641 701 985 1610 1704 1729
Size: 371 257 13 60 284 625 94 25
Cuts at: 1729 1907 1921 1972 2007
Size: 178 14 51 35
Fragments arranged by size:
625 371 284 257 178 94 60 51
35 25 14 13
BbeI G_GCGC'C
Cuts at: 0 1103 2007
Size: 1103 904
BbvCI CC'TCA_GC
Cuts at: 0 724 1677 2007
Size: 724 953 330
Fragments arranged by size:
953 724 330
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 754 788 1060 1094 2007
Size: 754 34 272 34 913
Fragments arranged by size:
913 754 272 34 34
BlpI GC'TnA_GC
Cuts at: 0 880 1645 1992 2007
Size: 880 765 347 15
Fragments arranged by size:
880 765 347 15
Bme1580I G_kGCm'C
Cuts at: 0 985 1366 1379 1921 1972 2007
Size: 985 381 13 542 51 35
Fragments arranged by size:
985 542 381 51 35 13
BmrI ACTGGGnnnn_n'
Cuts at: 0 715 981 2007
Size: 715 266 1026
Fragments arranged by size:
1026 715 266
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1092 2007
Size: 1092 915
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 385 599 715 1078 1580 1638 2007
Size: 298 87 214 116 363 502 58 369
Fragments arranged by size:
502 369 363 298 214 116 87 58
Bpu10I CC'TnA_GC
Cuts at: 0 724 1189 1436 1677 2007
Size: 724 465 247 241 330
Fragments arranged by size:
724 465 330 247 241
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 526 1624 2007
Size: 526 1098 383
Fragments arranged by size:
1098 526 383
BsaI GGTCTCn'nnnn_
Cuts at: 0 82 1030 1761 2007
Size: 82 948 731 246
Fragments arranged by size:
948 731 246 82
BsaHI Gr'CG_yC
Cuts at: 0 137 428 1100 2007
Size: 137 291 672 907
Fragments arranged by size:
907 672 291 137
BsaWI w'CCGG_w
Cuts at: 0 1039 2007
Size: 1039 968
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 430 2007
Size: 430 1577
BseYI C'CCAG_C
Cuts at: 0 6 440 619 1291 1625 1696 1758 1786
Size: 6 434 179 672 334 71 62 28
Cuts at: 1786 1876 1958 2007
Size: 90 82 49
Fragments arranged by size:
672 434 334 179 90 82 71 62
49 28 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1159 2007
Size: 1159 848
BsiHKAI G_wGCw'C
Cuts at: 0 299 371 662 1379 1572 1729 2007
Size: 299 72 291 717 193 157 278
Fragments arranged by size:
717 299 291 278 193 157 72
Bsp1286I G_dGCh'C
Cuts at: 0 299 371 628 641 662 701 985 1366
Size: 299 72 257 13 21 39 284 381
Cuts at: 1366 1379 1572 1610 1704 1729 1907 1921 1972
Size: 13 193 38 94 25 178 14 51
Cuts at: 1972 2007
Size: 35
Fragments arranged by size:
381 299 284 257 193 178 94 72
51 39 38 35 25 21 14 13
13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 197 609 2007
Size: 197 412 1398
Fragments arranged by size:
1398 412 197
BsrBI CCG'CTC
Cuts at: 0 483 2007
Size: 483 1524
BsrFI r'CCGG_y
Cuts at: 0 74 2007
Size: 74 1933
BssHII G'CGCG_C
Cuts at: 0 18 2007
Size: 18 1989
BstYI r'GATC_y
Cuts at: 0 1160 2007
Size: 1160 847
BtgI C'CryG_G
Cuts at: 0 32 132 476 522 1484 1581 1691 2007
Size: 32 100 344 46 962 97 110 316
Fragments arranged by size:
962 344 316 110 100 97 46 32
BtsI GCAGTG_nn'
Cuts at: 0 655 1418 1924 2007
Size: 655 763 506 83
Fragments arranged by size:
763 655 506 83
EaeI y'GGCC_r
Cuts at: 0 548 1205 1304 2007
Size: 548 657 99 703
Fragments arranged by size:
703 657 548 99
EarI CTCTTCn'nnn_
Cuts at: 0 499 1028 1390 1702 2007
Size: 499 529 362 312 305
Fragments arranged by size:
529 499 362 312 305
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 807 2007
Size: 807 1200
EcoICRI GAG'CTC
Cuts at: 0 369 1727 2007
Size: 369 1358 280
Fragments arranged by size:
1358 369 280
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 289 298 385 406 599 715 1078 1259
Size: 289 9 87 21 193 116 363 181
Cuts at: 1259 1580 1638 2007
Size: 321 58 369
Fragments arranged by size:
369 363 321 289 193 181 116 87
58 21 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 154 1192 1664 2007
Size: 154 1038 472 343
Fragments arranged by size:
1038 472 343 154
EcoO109I rG'GnC_Cy
Cuts at: 0 273 471 587 716 981 982 1255 1529
Size: 273 198 116 129 265 1 273 274
Cuts at: 1529 1671 1712 1781 1872 1918 1968 1969 2007
Size: 142 41 69 91 46 50 1 38
Fragments arranged by size:
274 273 273 265 198 142 129 116
91 69 50 46 41 38 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 436 2007
Size: 436 1571
FspI TGC'GCA
Cuts at: 0 43 193 499 605 2007
Size: 43 150 306 106 1402
Fragments arranged by size:
1402 306 150 106 43
HaeII r_GCGC'y
Cuts at: 0 7 146 1103 2007
Size: 7 139 957 904
Fragments arranged by size:
957 904 139 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 145 177 368 400 2007
Size: 145 32 191 32 1607
Fragments arranged by size:
1607 191 145 32 32
HincII GTy'rAC
Cuts at: 0 29 2007
Size: 29 1978
KasI G'GCGC_C
Cuts at: 0 1099 2007
Size: 1099 908
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 308 872 971 2007
Size: 308 564 99 1036
Fragments arranged by size:
1036 564 308 99
MscI TGG'CCA
Cuts at: 0 1306 2007
Size: 1306 701
MslI CAynn'nnrTG
Cuts at: 0 404 964 1483 2007
Size: 404 560 519 524
Fragments arranged by size:
560 524 519 404
MspA1I CmG'CkG
Cuts at: 0 108 134 363 524 619 645 1095 1210
Size: 108 26 229 161 95 26 450 115
Cuts at: 1210 1302 1625 2007
Size: 92 323 382
Fragments arranged by size:
450 382 323 229 161 115 108 95
92 26 26
NaeI GCC'GGC
Cuts at: 0 76 2007
Size: 76 1931
NarI GG'CG_CC
Cuts at: 0 1100 2007
Size: 1100 907
NcoI C'CATG_G
Cuts at: 0 1484 1581 1691 2007
Size: 1484 97 110 316
Fragments arranged by size:
1484 316 110 97
NdeI CA'TA_TG
Cuts at: 0 581 2007
Size: 581 1426
NgoMIV G'CCGG_C
Cuts at: 0 74 2007
Size: 74 1933
NspI r_CATG'y
Cuts at: 0 198 2007
Size: 198 1809
PasI CC'CwG_GG
Cuts at: 0 1129 1526 1858 1908 2007
Size: 1129 397 332 50 99
Fragments arranged by size:
1129 397 332 99 50
PflMI CCAn_nnn'nTGG
Cuts at: 0 738 840 2007
Size: 738 102 1167
Fragments arranged by size:
1167 738 102
PfoI T'CCnGG_A
Cuts at: 0 454 1262 1393 2007
Size: 454 808 131 614
Fragments arranged by size:
808 614 454 131
PpuMI rG'GwC_Cy
Cuts at: 0 1255 1671 1712 1781 1872 2007
Size: 1255 416 41 69 91 135
Fragments arranged by size:
1255 416 135 91 69 41
PshAI GACnn'nnGTC
Cuts at: 0 1241 2007
Size: 1241 766
PspOMI G'GGCC_C
Cuts at: 0 981 1917 1968 2007
Size: 981 936 51 39
Fragments arranged by size:
981 936 51 39
PstI C_TGCA'G
Cuts at: 0 17 650 1231 1871 2007
Size: 17 633 581 640 136
Fragments arranged by size:
640 633 581 136 17
PvuII CAG'CTG
Cuts at: 0 108 363 619 2007
Size: 108 255 256 1388
Fragments arranged by size:
1388 256 255 108
SacI G_AGCT'C
Cuts at: 0 371 1729 2007
Size: 371 1358 278
Fragments arranged by size:
1358 371 278
SacII CC_GC'GG
Cuts at: 0 135 525 2007
Size: 135 390 1482
Fragments arranged by size:
1482 390 135
SalI G'TCGA_C
Cuts at: 0 27 2007
Size: 27 1980
SanDI GG'GwC_CC
Cuts at: 0 1255 1671 2007
Size: 1255 416 336
Fragments arranged by size:
1255 416 336
SbfI CC_TGCA'GG
Cuts at: 0 1871 2007
Size: 1871 136
SexAI A'CCwGG_T
Cuts at: 0 227 2007
Size: 227 1780
SfcI C'TryA_G
Cuts at: 0 13 646 789 1227 1867 2007
Size: 13 633 143 438 640 140
Fragments arranged by size:
640 633 438 143 140 13
SfoI GGC'GCC
Cuts at: 0 1101 2007
Size: 1101 906
SmaI CCC'GGG
Cuts at: 0 58 1965 2007
Size: 58 1907 42
Fragments arranged by size:
1907 58 42
SmlI C'TyrA_G
Cuts at: 0 541 1603 2007
Size: 541 1062 404
Fragments arranged by size:
1062 541 404
SphI G_CATG'C
Cuts at: 0 198 2007
Size: 198 1809
StuI AGG'CCT
Cuts at: 0 160 2007
Size: 160 1847
StyI C'CwwG_G
Cuts at: 0 1484 1581 1691 1981 2007
Size: 1484 97 110 290 26
Fragments arranged by size:
1484 290 110 97 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 106 698 2007
Size: 106 592 1309
Fragments arranged by size:
1309 592 106
TatI w'GTAC_w
Cuts at: 0 827 2007
Size: 827 1180
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1168 1200 2007
Size: 1168 32 807
Fragments arranged by size:
1168 807 32
Tth111I GACn'n_nGTC
Cuts at: 0 450 853 2007
Size: 450 403 1154
Fragments arranged by size:
1154 450 403
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 462 1299 2007
Size: 462 837 708
Fragments arranged by size:
837 708 462
XmaI C'CCGG_G
Cuts at: 0 56 1963 2007
Size: 56 1907 44
Fragments arranged by size:
1907 56 44
XmnI GAAnn'nnTTC
Cuts at: 0 1805 2007
Size: 1805 202
Enzymes that do cut:
AarI AccI AcuI AleI AloI AlwNI ApaI ApaLI
AvaI BamHI BanI BanII BbeI BbvCI BcgI BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI
BsaWI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrBI
BsrFI BssHII BstYI BtgI BtsI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I
HincII KasI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PasI PflMI PfoI PpuMI
PshAI PspOMI PstI PvuII SacI SacII SalI SanDI
SbfI SexAI SfcI SfoI SmaI SmlI SphI StuI
StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI ApoI AscI AseI AsiSI AvrII BaeI BbsI
BciVI BclI BfrBI BglI BglII BmgBI BmtI BsaAI
BsaBI BsaXI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BsrDI BsrGI BssSI BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI
EcoRI EcoRV FseI FspAI HindIII HpaI KpnI MfeI
MluI NheI NotI NruI NsiI PacI PciI PmeI
PmlI PpiI PsiI PspXI PsrI PvuI RsrII SapI
ScaI SfiI SgfI SgrAI SnaBI SpeI SrfI SspI
SwaI XbaI XhoI ZraI