Restriction map for
pF1KB6223
(Linear) (Six-base) MAPSORT of: KIBB6223.seq Check: 6958 from: 1 to: 2010
KIBB6223.orf
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AatII G_ACGT'C
Cuts at: 0 984 2010
Size: 984 1026
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 411 758 2010
Size: 411 347 1252
Fragments arranged by size:
1252 411 347
AfeI AGC'GCT
Cuts at: 0 1627 2010
Size: 1627 383
AflIII A'CryG_T
Cuts at: 0 189 2010
Size: 189 1821
AleI CACnn'nnGTG
Cuts at: 0 308 1409 2010
Size: 308 1101 601
Fragments arranged by size:
1101 601 308
ApoI r'AATT_y
Cuts at: 0 812 961 1316 1459 1568 2010
Size: 812 149 355 143 109 442
Fragments arranged by size:
812 442 355 149 143 109
BanII G_rGCy'C
Cuts at: 0 46 134 476 1798 2010
Size: 46 88 342 1322 212
Fragments arranged by size:
1322 342 212 88 46
BbvCI CC'TCA_GC
Cuts at: 0 64 2010
Size: 64 1946
BlpI GC'TnA_GC
Cuts at: 0 359 2010
Size: 359 1651
BmrI ACTGGGnnnn_n'
Cuts at: 0 325 593 2010
Size: 325 268 1417
Fragments arranged by size:
1417 325 268
BmtI G_CTAG'C
Cuts at: 0 1718 2010
Size: 1718 292
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 926 1010 1094 1178 1262 1514 1682
Size: 196 730 84 84 84 84 252 168
Cuts at: 1682 1766 1850 1934 2010
Size: 84 84 84 76
Fragments arranged by size:
730 252 196 168 84 84 84 84
84 84 84 76
Bpu10I CC'TnA_GC
Cuts at: 0 64 77 2010
Size: 64 13 1933
Fragments arranged by size:
1933 64 13
BsaHI Gr'CG_yC
Cuts at: 0 981 2010
Size: 981 1029
BsaWI w'CCGG_w
Cuts at: 0 1562 1646 2010
Size: 1562 84 364
Fragments arranged by size:
1562 364 84
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 622 1007 2010
Size: 622 385 1003
Fragments arranged by size:
1003 622 385
BseYI C'CCAG_C
Cuts at: 0 561 2010
Size: 561 1449
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1281 1449 1617 1869 2010
Size: 1281 168 168 252 141
Fragments arranged by size:
1281 252 168 168 141
BsiEI CG_ry'CG
Cuts at: 0 14 18 2010
Size: 14 4 1992
Fragments arranged by size:
1992 14 4
BsiHKAI G_wGCw'C
Cuts at: 0 46 134 365 1798 2010
Size: 46 88 231 1433 212
Fragments arranged by size:
1433 231 212 88 46
BsmI GAATG_Cn'
Cuts at: 0 928 1348 1400 1516 1936 2010
Size: 928 420 52 116 420 74
Fragments arranged by size:
928 420 420 116 74 52
BsmBI CGTCTCn'nnnn_
Cuts at: 0 972 2010
Size: 972 1038
Bsp1286I G_dGCh'C
Cuts at: 0 46 134 365 476 1798 2010
Size: 46 88 231 111 1322 212
Fragments arranged by size:
1322 231 212 111 88 46
BspHI T'CATG_A
Cuts at: 0 1002 2010
Size: 1002 1008
BsrBI CCG'CTC
Cuts at: 0 23 2010
Size: 23 1987
BsrFI r'CCGG_y
Cuts at: 0 511 2010
Size: 511 1499
BstAPI GCAn_nnn'nTGC
Cuts at: 0 872 2010
Size: 872 1138
BtgI C'CryG_G
Cuts at: 0 37 213 569 2010
Size: 37 176 356 1441
Fragments arranged by size:
1441 356 176 37
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 179 2010
Size: 179 1831
BtsI GCAGTG_nn'
Cuts at: 0 933 1269 1437 1521 1605 1941 2010
Size: 933 336 168 84 84 336 69
Fragments arranged by size:
933 336 336 168 84 84 69
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 106 141 2010
Size: 106 35 1869
Fragments arranged by size:
1869 106 35
EaeI y'GGCC_r
Cuts at: 0 15 101 2010
Size: 15 86 1909
Fragments arranged by size:
1909 86 15
EagI C'GGCC_G
Cuts at: 0 15 2010
Size: 15 1995
EarI CTCTTCn'nnn_
Cuts at: 0 116 464 1224 2010
Size: 116 348 760 786
Fragments arranged by size:
786 760 348 116
EcoICRI GAG'CTC
Cuts at: 0 44 132 1796 2010
Size: 44 88 1664 214
Fragments arranged by size:
1664 214 88 44
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 411 758 926 1010 1094 1178 1262
Size: 196 215 347 168 84 84 84 84
Cuts at: 1262 1514 1682 1766 1850 1934 2010
Size: 252 168 84 84 84 76
Fragments arranged by size:
347 252 215 196 168 168 84 84
84 84 84 84 84 76
EcoNI CCTnn'n_nnAGG
Cuts at: 0 480 2010
Size: 480 1530
EcoRI G'AATT_C
Cuts at: 0 961 1316 1568 2010
Size: 961 355 252 442
Fragments arranged by size:
961 442 355 252
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 411 763 2010
Size: 411 352 1247
Fragments arranged by size:
1247 411 352
HaeII r_GCGC'y
Cuts at: 0 1629 2010
Size: 1629 381
HindIII A'AGCT_T
Cuts at: 0 689 2010
Size: 689 1321
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 606 2010
Size: 606 1404
MscI TGG'CCA
Cuts at: 0 103 2010
Size: 103 1907
MslI CAynn'nnrTG
Cuts at: 0 308 409 461 725 1007 1409 2010
Size: 308 101 52 264 282 402 601
Fragments arranged by size:
601 402 308 282 264 101 52
NcoI C'CATG_G
Cuts at: 0 213 2010
Size: 213 1797
NheI G'CTAG_C
Cuts at: 0 1714 2010
Size: 1714 296
NspI r_CATG'y
Cuts at: 0 193 2010
Size: 193 1817
PasI CC'CwG_GG
Cuts at: 0 201 2010
Size: 201 1809
PciI A'CATG_T
Cuts at: 0 189 2010
Size: 189 1821
PflMI CCAn_nnn'nTGG
Cuts at: 0 219 2010
Size: 219 1791
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 717 749 2010
Size: 717 32 1261
Fragments arranged by size:
1261 717 32
PstI C_TGCA'G
Cuts at: 0 520 2010
Size: 520 1490
SacI G_AGCT'C
Cuts at: 0 46 134 1798 2010
Size: 46 88 1664 212
Fragments arranged by size:
1664 212 88 46
SapI GCTCTTCn'nnn_
Cuts at: 0 464 2010
Size: 464 1546
SfcI C'TryA_G
Cuts at: 0 516 2010
Size: 516 1494
StuI AGG'CCT
Cuts at: 0 556 915 1083 1167 1419 1671 1755 1923
Size: 556 359 168 84 252 252 84 168
Cuts at: 1923 2007 2010
Size: 84 3
Fragments arranged by size:
556 359 252 252 168 168 84 84
84 3
StyI C'CwwG_G
Cuts at: 0 213 2010
Size: 213 1797
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 303 307 2010
Size: 303 4 1703
Fragments arranged by size:
1703 303 4
TatI w'GTAC_w
Cuts at: 0 158 2010
Size: 158 1852
XmnI GAAnn'nnTTC
Cuts at: 0 538 690 775 965 1301 2010
Size: 538 152 85 190 336 709
Fragments arranged by size:
709 538 336 190 152 85
ZraI GAC'GTC
Cuts at: 0 982 2010
Size: 982 1028
Enzymes that do cut:
AatII AcuI AfeI AflIII AleI ApoI BanII BbvCI
BlpI BmrI BmtI BpmI Bpu10I BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BsrBI BsrFI BstAPI BtgI BtgZI BtsI CspCI EaeI
EagI EarI EcoICRI Eco57MI EcoNI EcoRI FalI HaeII
HindIII MmeI MscI MslI NcoI NheI NspI PasI
PciI PflMI PpiI PstI SacI SapI SfcI StuI
StyI TaqII TatI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AflII AgeI AhdI AlfI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BbsI BcgI BciVI
BclI BfrBI BglI BglII Bme1580I BmgBI BplI BpuEI
BsaI BsaAI BsaBI BsaXI BsiWI BspEI BspMI BsrDI
BsrGI BssHII BssSI BstBI BstEII BstXI BstYI BstZ17I
Bsu36I ClaI DraI DraIII DrdI EciI EcoO109I EcoRV
FseI FspI FspAI Hin4I HincII HpaI KasI KpnI
MfeI MluI MspA1I NaeI NarI NdeI NgoMIV NotI
NruI NsiI PacI PfoI PmeI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
SwaI TstI Tth111I XbaI XcmI XhoI XmaI