Restriction map for
pF1KB6232
(Linear) (Six-base) MAPSORT of: KIBB6232.seq Check: 8536 from: 1 to: 2028
KIBB6232.orf
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AfeI AGC'GCT
Cuts at: 0 935 2028
Size: 935 1093
AflIII A'CryG_T
Cuts at: 0 630 2028
Size: 630 1398
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 374 1165 1340 2028
Size: 374 791 175 688
Fragments arranged by size:
791 688 374 175
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 940 972 2028
Size: 940 32 1056
Fragments arranged by size:
1056 940 32
AlwNI CAG_nnn'CTG
Cuts at: 0 262 1923 2028
Size: 262 1661 105
Fragments arranged by size:
1661 262 105
ApaI G_GGCC'C
Cuts at: 0 15 62 116 1179 2028
Size: 15 47 54 1063 849
Fragments arranged by size:
1063 849 54 47 15
ApaLI G'TGCA_C
Cuts at: 0 645 1649 2028
Size: 645 1004 379
Fragments arranged by size:
1004 645 379
AvaI C'yCGr_G
Cuts at: 0 55 142 590 914 1012 1117 1396 1882
Size: 55 87 448 324 98 105 279 486
Cuts at: 1882 2028
Size: 146
Fragments arranged by size:
486 448 324 279 146 105 98 87
55
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 619 652 2028
Size: 619 33 1376
Fragments arranged by size:
1376 619 33
BanI G'GyrC_C
Cuts at: 0 64 267 586 1986 2028
Size: 64 203 319 1400 42
Fragments arranged by size:
1400 319 203 64 42
BanII G_rGCy'C
Cuts at: 0 15 53 62 116 517 847 1179 1324
Size: 15 38 9 54 401 330 332 145
Cuts at: 1324 1538 2028
Size: 214 490
Fragments arranged by size:
490 401 332 330 214 145 54 38
15 9
BbeI G_GCGC'C
Cuts at: 0 68 1990 2028
Size: 68 1922 38
Fragments arranged by size:
1922 68 38
BbvCI CC'TCA_GC
Cuts at: 0 25 2006 2028
Size: 25 1981 22
Fragments arranged by size:
1981 25 22
BglI GCCn_nnn'nGGC
Cuts at: 0 57 1174 1797 2028
Size: 57 1117 623 231
Fragments arranged by size:
1117 623 231 57
BlpI GC'TnA_GC
Cuts at: 0 776 874 1285 2028
Size: 776 98 411 743
Fragments arranged by size:
776 743 411 98
Bme1580I G_kGCm'C
Cuts at: 0 15 62 116 344 591 649 1179 1653
Size: 15 47 54 228 247 58 530 474
Cuts at: 1653 2028
Size: 375
Fragments arranged by size:
530 474 375 247 228 58 54 47
15
BmgBI CAC'GTC
Cuts at: 0 1722 2028
Size: 1722 306
BmrI ACTGGGnnnn_n'
Cuts at: 0 491 2028
Size: 491 1537
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1338 2028
Size: 1338 690
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 501 820 1081 1111 1397 1576 2002 2028
Size: 501 319 261 30 286 179 426 26
Fragments arranged by size:
501 426 319 286 261 179 30 26
Bpu10I CC'TnA_GC
Cuts at: 0 25 511 1483 2006 2028
Size: 25 486 972 523 22
Fragments arranged by size:
972 523 486 25 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 2028
Size: 229 1799
BsaI GGTCTCn'nnnn_
Cuts at: 0 911 2028
Size: 911 1117
BsaAI yAC'GTr
Cuts at: 0 537 2028
Size: 537 1491
BsaBI GATnn'nnATC
Cuts at: 0 449 2028
Size: 449 1579
BsaHI Gr'CG_yC
Cuts at: 0 65 138 164 1421 1592 1987 2028
Size: 65 73 26 1257 171 395 41
Fragments arranged by size:
1257 395 171 73 65 41 26
BsaWI w'CCGG_w
Cuts at: 0 17 374 1841 2028
Size: 17 357 1467 187
Fragments arranged by size:
1467 357 187 17
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2005 2028
Size: 2005 23
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 775 1573 2028
Size: 775 798 455
Fragments arranged by size:
798 775 455
BseYI C'CCAG_C
Cuts at: 0 501 1238 1602 1897 2028
Size: 501 737 364 295 131
Fragments arranged by size:
737 501 364 295 131
BsiEI CG_ry'CG
Cuts at: 0 453 2028
Size: 453 1575
BsiHKAI G_wGCw'C
Cuts at: 0 517 545 649 755 1324 1640 1653 2028
Size: 517 28 104 106 569 316 13 375
Fragments arranged by size:
569 517 375 316 106 104 28 13
Bsp1286I G_dGCh'C
Cuts at: 0 15 53 62 116 344 517 545 591
Size: 15 38 9 54 228 173 28 46
Cuts at: 591 649 755 847 1179 1324 1538 1640 1653
Size: 58 106 92 332 145 214 102 13
Cuts at: 1653 2028
Size: 375
Fragments arranged by size:
375 332 228 214 173 145 106 102
92 58 54 46 38 28 15 13
9
BspEI T'CCGG_A
Cuts at: 0 17 374 2028
Size: 17 357 1654
Fragments arranged by size:
1654 357 17
BsrDI GCAATG_nn'
Cuts at: 0 1826 2028
Size: 1826 202
BsrFI r'CCGG_y
Cuts at: 0 992 1625 2028
Size: 992 633 403
Fragments arranged by size:
992 633 403
BsrGI T'GTAC_A
Cuts at: 0 938 2028
Size: 938 1090
BssSI C'ACGA_G
Cuts at: 0 1570 1993 2028
Size: 1570 423 35
Fragments arranged by size:
1570 423 35
BtgI C'CryG_G
Cuts at: 0 616 797 1607 1815 1865 2028
Size: 616 181 810 208 50 163
Fragments arranged by size:
810 616 208 181 163 50
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 893 2028
Size: 893 1135
BtsI GCAGTG_nn'
Cuts at: 0 1413 1592 2028
Size: 1413 179 436
Fragments arranged by size:
1413 436 179
ClaI AT'CG_AT
Cuts at: 0 449 2028
Size: 449 1579
DrdI GACnn_nn'nnGTC
Cuts at: 0 1731 2028
Size: 1731 297
EaeI y'GGCC_r
Cuts at: 0 42 794 1958 2028
Size: 42 752 1164 70
Fragments arranged by size:
1164 752 70 42
EarI CTCTTCn'nnn_
Cuts at: 0 1210 1840 2028
Size: 1210 630 188
Fragments arranged by size:
1210 630 188
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1189 1644 2028
Size: 1189 455 384
Fragments arranged by size:
1189 455 384
EcoICRI GAG'CTC
Cuts at: 0 515 1322 2028
Size: 515 807 706
Fragments arranged by size:
807 706 515
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 501 820 1081 1111 1397 1576 2002 2028
Size: 501 319 261 30 286 179 426 26
Fragments arranged by size:
501 426 319 286 261 179 30 26
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1207 1785 2028
Size: 1207 578 243
Fragments arranged by size:
1207 578 243
EcoO109I rG'GnC_Cy
Cuts at: 0 12 112 986 1131 1599 1920 2028
Size: 12 100 874 145 468 321 108
Fragments arranged by size:
874 468 321 145 108 100 12
EcoRV GAT'ATC
Cuts at: 0 447 2028
Size: 447 1581
HaeII r_GCGC'y
Cuts at: 0 68 937 1090 1363 1990 2028
Size: 68 869 153 273 627 38
Fragments arranged by size:
869 627 273 153 68 38
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1788 1820 2028
Size: 1788 32 208
Fragments arranged by size:
1788 208 32
HincII GTy'rAC
Cuts at: 0 174 858 2028
Size: 174 684 1170
Fragments arranged by size:
1170 684 174
KasI G'GCGC_C
Cuts at: 0 64 1986 2028
Size: 64 1922 42
Fragments arranged by size:
1922 64 42
MscI TGG'CCA
Cuts at: 0 1960 2028
Size: 1960 68
MslI CAynn'nnrTG
Cuts at: 0 362 1670 2028
Size: 362 1308 358
Fragments arranged by size:
1308 362 358
MspA1I CmG'CkG
Cuts at: 0 1491 2028
Size: 1491 537
NaeI GCC'GGC
Cuts at: 0 994 2028
Size: 994 1034
NarI GG'CG_CC
Cuts at: 0 65 1987 2028
Size: 65 1922 41
Fragments arranged by size:
1922 65 41
NcoI C'CATG_G
Cuts at: 0 1815 1865 2028
Size: 1815 50 163
Fragments arranged by size:
1815 163 50
NgoMIV G'CCGG_C
Cuts at: 0 992 2028
Size: 992 1036
NspI r_CATG'y
Cuts at: 0 1146 2028
Size: 1146 882
PflMI CCAn_nnn'nTGG
Cuts at: 0 1550 2028
Size: 1550 478
PfoI T'CCnGG_A
Cuts at: 0 517 1058 1208 2028
Size: 517 541 150 820
Fragments arranged by size:
820 541 517 150
PpuMI rG'GwC_Cy
Cuts at: 0 1131 1599 2028
Size: 1131 468 429
Fragments arranged by size:
1131 468 429
PspOMI G'GGCC_C
Cuts at: 0 11 58 112 1175 2028
Size: 11 47 54 1063 853
Fragments arranged by size:
1063 853 54 47 11
PspXI vC'TCGA_Gb
Cuts at: 0 1396 2028
Size: 1396 632
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1174 1206 2028
Size: 1174 32 822
Fragments arranged by size:
1174 822 32
PstI C_TGCA'G
Cuts at: 0 872 2028
Size: 872 1156
PvuI CG_AT'CG
Cuts at: 0 453 2028
Size: 453 1575
PvuII CAG'CTG
Cuts at: 0 1491 2028
Size: 1491 537
SacI G_AGCT'C
Cuts at: 0 517 1324 2028
Size: 517 807 704
Fragments arranged by size:
807 704 517
SanDI GG'GwC_CC
Cuts at: 0 1599 2028
Size: 1599 429
ScaI AGT'ACT
Cuts at: 0 1096 2028
Size: 1096 932
SexAI A'CCwGG_T
Cuts at: 0 693 2028
Size: 693 1335
SfcI C'TryA_G
Cuts at: 0 684 868 2028
Size: 684 184 1160
Fragments arranged by size:
1160 684 184
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1174 2028
Size: 1174 854
SfoI GGC'GCC
Cuts at: 0 66 1988 2028
Size: 66 1922 40
Fragments arranged by size:
1922 66 40
SmaI CCC'GGG
Cuts at: 0 144 592 2028
Size: 144 448 1436
Fragments arranged by size:
1436 448 144
SmlI C'TyrA_G
Cuts at: 0 208 914 1117 1396 2028
Size: 208 706 203 279 632
Fragments arranged by size:
706 632 279 208 203
SphI G_CATG'C
Cuts at: 0 1146 2028
Size: 1146 882
SrfI GCCC'GGGC
Cuts at: 0 592 2028
Size: 592 1436
StuI AGG'CCT
Cuts at: 0 731 1339 2028
Size: 731 608 689
Fragments arranged by size:
731 689 608
StyI C'CwwG_G
Cuts at: 0 1506 1815 1865 2028
Size: 1506 309 50 163
Fragments arranged by size:
1506 309 163 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1668 2028
Size: 1668 360
TatI w'GTAC_w
Cuts at: 0 938 1094 2028
Size: 938 156 934
Fragments arranged by size:
938 934 156
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2005 2028
Size: 2005 23
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 711 1551 2028
Size: 711 840 477
Fragments arranged by size:
840 711 477
XhoI C'TCGA_G
Cuts at: 0 914 1117 1396 2028
Size: 914 203 279 632
Fragments arranged by size:
914 632 279 203
XmaI C'CCGG_G
Cuts at: 0 142 590 2028
Size: 142 448 1438
Fragments arranged by size:
1438 448 142
Enzymes that do cut:
AfeI AflIII AlfI AloI AlwNI ApaI ApaLI AvaI
BaeI BanI BanII BbeI BbvCI BglI BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
Bsp1286I BspEI BsrDI BsrFI BsrGI BssSI BtgI BtgZI
BtsI ClaI DrdI EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRV HaeII Hin4I HincII KasI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PflMI
PfoI PpuMI PspOMI PspXI PsrI PstI PvuI PvuII
SacI SanDI ScaI SexAI SfcI SfiI SfoI SmaI
SmlI SphI SrfI StuI StyI TaqII TatI TstI
XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AgeI
AhdI AleI ApoI AscI AseI AsiSI AvrII BamHI
BbsI BcgI BciVI BclI BfrBI BglII BmtI BsgI
BsiWI BsmI BsmBI BspHI BspMI BsrBI BssHII BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I CspCI DraI
DraIII EagI EcoRI FalI FseI FspI FspAI HindIII
HpaI KpnI MfeI MluI MmeI NdeI NheI NotI
NruI NsiI PacI PasI PciI PmeI PmlI PpiI
PshAI PsiI RsrII SacII SalI SapI SbfI SgfI
SgrAI SnaBI SpeI SspI SwaI Tth111I XbaI XmnI
ZraI