Restriction map for
pF1KB6240
(Linear) (Six-base) MAPSORT of: KIBB6240.seq Check: 5746 from: 1 to: 2031
KIBB6240.orf
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 507 1112 2031
Size: 507 605 919
Fragments arranged by size:
919 605 507
AccI GT'mk_AC
Cuts at: 0 1117 1743 2031
Size: 1117 626 288
Fragments arranged by size:
1117 626 288
AclI AA'CG_TT
Cuts at: 0 1583 2031
Size: 1583 448
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 193 262 1955 2031
Size: 169 24 69 1693 76
Fragments arranged by size:
1693 169 76 69 24
AflIII A'CryG_T
Cuts at: 0 769 1899 2031
Size: 769 1130 132
Fragments arranged by size:
1130 769 132
ApaI G_GGCC'C
Cuts at: 0 1943 2031
Size: 1943 88
ApoI r'AATT_y
Cuts at: 0 1369 1618 2031
Size: 1369 249 413
Fragments arranged by size:
1369 413 249
AvaI C'yCGr_G
Cuts at: 0 625 2031
Size: 625 1406
BanII G_rGCy'C
Cuts at: 0 367 1916 1943 2031
Size: 367 1549 27 88
Fragments arranged by size:
1549 367 88 27
BbsI GAAGACnn'nnnn_
Cuts at: 0 85 1546 2031
Size: 85 1461 485
Fragments arranged by size:
1461 485 85
BbvCI CC'TCA_GC
Cuts at: 0 368 1139 2031
Size: 368 771 892
Fragments arranged by size:
892 771 368
BfrBI ATG'CAT
Cuts at: 0 1411 2031
Size: 1411 620
BglII A'GATC_T
Cuts at: 0 1469 1870 2031
Size: 1469 401 161
Fragments arranged by size:
1469 401 161
BlpI GC'TnA_GC
Cuts at: 0 361 2031
Size: 361 1670
Bme1580I G_kGCm'C
Cuts at: 0 1337 1943 2031
Size: 1337 606 88
Fragments arranged by size:
1337 606 88
BmgBI CAC'GTC
Cuts at: 0 1902 2031
Size: 1902 129
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1226 2031
Size: 1226 805
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 361 373 1427 2031
Size: 331 30 12 1054 604
Fragments arranged by size:
1054 604 331 30 12
Bpu10I CC'TnA_GC
Cuts at: 0 368 1139 2031
Size: 368 771 892
Fragments arranged by size:
892 771 368
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 644 2031
Size: 296 348 1387
Fragments arranged by size:
1387 348 296
BsaAI yAC'GTr
Cuts at: 0 1523 2031
Size: 1523 508
BsaBI GATnn'nnATC
Cuts at: 0 243 2031
Size: 243 1788
BsaHI Gr'CG_yC
Cuts at: 0 121 2031
Size: 121 1910
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 91 121 2031
Size: 91 30 1910
Fragments arranged by size:
1910 91 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 376 768 771 895 2031
Size: 376 392 3 124 1136
Fragments arranged by size:
1136 392 376 124 3
BseYI C'CCAG_C
Cuts at: 0 161 326 527 2031
Size: 161 165 201 1504
Fragments arranged by size:
1504 201 165 161
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 770 1680 2031
Size: 770 910 351
Fragments arranged by size:
910 770 351
BsiEI CG_ry'CG
Cuts at: 0 128 2031
Size: 128 1903
BsiHKAI G_wGCw'C
Cuts at: 0 714 887 2031
Size: 714 173 1144
Fragments arranged by size:
1144 714 173
BsmI GAATG_Cn'
Cuts at: 0 1536 2031
Size: 1536 495
BsmBI CGTCTCn'nnnn_
Cuts at: 0 112 2031
Size: 112 1919
Bsp1286I G_dGCh'C
Cuts at: 0 367 714 887 1337 1916 1943 2031
Size: 367 347 173 450 579 27 88
Fragments arranged by size:
579 450 367 347 173 88 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 507 1026 1112 2031
Size: 507 519 86 919
Fragments arranged by size:
919 519 507 86
BsrDI GCAATG_nn'
Cuts at: 0 1832 2031
Size: 1832 199
BsrFI r'CCGG_y
Cuts at: 0 123 2031
Size: 123 1908
BsrGI T'GTAC_A
Cuts at: 0 1041 1896 2031
Size: 1041 855 135
Fragments arranged by size:
1041 855 135
BstAPI GCAn_nnn'nTGC
Cuts at: 0 357 1354 1887 2031
Size: 357 997 533 144
Fragments arranged by size:
997 533 357 144
BstEII G'GTnAC_C
Cuts at: 0 1651 2031
Size: 1651 380
BstYI r'GATC_y
Cuts at: 0 341 756 1469 1870 2031
Size: 341 415 713 401 161
Fragments arranged by size:
713 415 401 341 161
BstZ17I GTA'TAC
Cuts at: 0 1744 2031
Size: 1744 287
BtgI C'CryG_G
Cuts at: 0 128 1766 2031
Size: 128 1638 265
Fragments arranged by size:
1638 265 128
BtsI GCAGTG_nn'
Cuts at: 0 950 1541 2031
Size: 950 591 490
Fragments arranged by size:
950 591 490
DraIII CAC_nnn'GTG
Cuts at: 0 1293 2031
Size: 1293 738
EaeI y'GGCC_r
Cuts at: 0 125 928 1517 2031
Size: 125 803 589 514
Fragments arranged by size:
803 589 514 125
EagI C'GGCC_G
Cuts at: 0 125 2031
Size: 125 1906
EarI CTCTTCn'nnn_
Cuts at: 0 281 1805 1904 2031
Size: 281 1524 99 127
Fragments arranged by size:
1524 281 127 99
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 193 262 331 361 373 1427 1955
Size: 169 24 69 69 30 12 1054 528
Cuts at: 1955 2031
Size: 76
Fragments arranged by size:
1054 528 169 76 69 69 30 24
12
EcoO109I rG'GnC_Cy
Cuts at: 0 402 1258 1297 1939 1940 2031
Size: 402 856 39 642 1 91
Fragments arranged by size:
856 642 402 91 39 1
EcoRI G'AATT_C
Cuts at: 0 1618 2031
Size: 1618 413
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 819 2031
Size: 819 1212
FspI TGC'GCA
Cuts at: 0 1358 2031
Size: 1358 673
HaeII r_GCGC'y
Cuts at: 0 603 2031
Size: 603 1428
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 91 123 294 326 696 728 946 978
Size: 91 32 171 32 370 32 218 32
Cuts at: 978 2031
Size: 1053
Fragments arranged by size:
1053 370 218 171 91 32 32 32
32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 697 1243 1530 2031
Size: 134 563 546 287 501
Fragments arranged by size:
563 546 501 287 134
MscI TGG'CCA
Cuts at: 0 930 2031
Size: 930 1101
MslI CAynn'nnrTG
Cuts at: 0 1106 1282 1489 2031
Size: 1106 176 207 542
Fragments arranged by size:
1106 542 207 176
MspA1I CmG'CkG
Cuts at: 0 145 2031
Size: 145 1886
NaeI GCC'GGC
Cuts at: 0 125 2031
Size: 125 1906
NcoI C'CATG_G
Cuts at: 0 1766 2031
Size: 1766 265
NdeI CA'TA_TG
Cuts at: 0 1407 2031
Size: 1407 624
NgoMIV G'CCGG_C
Cuts at: 0 123 2031
Size: 123 1908
NsiI A_TGCA'T
Cuts at: 0 1413 2031
Size: 1413 618
NspI r_CATG'y
Cuts at: 0 490 773 823 994 1111 1287 1677 2031
Size: 490 283 50 171 117 176 390 354
Fragments arranged by size:
490 390 354 283 176 171 117 50
PciI A'CATG_T
Cuts at: 0 769 2031
Size: 769 1262
PflMI CCAn_nnn'nTGG
Cuts at: 0 534 2031
Size: 534 1497
PmlI CAC'GTG
Cuts at: 0 1523 2031
Size: 1523 508
PpuMI rG'GwC_Cy
Cuts at: 0 1297 2031
Size: 1297 734
PshAI GACnn'nnGTC
Cuts at: 0 772 1246 1859 2031
Size: 772 474 613 172
Fragments arranged by size:
772 613 474 172
PsiI TTA'TAA
Cuts at: 0 440 1376 2031
Size: 440 936 655
Fragments arranged by size:
936 655 440
PspOMI G'GGCC_C
Cuts at: 0 1939 2031
Size: 1939 92
PstI C_TGCA'G
Cuts at: 0 686 723 2031
Size: 686 37 1308
Fragments arranged by size:
1308 686 37
PvuII CAG'CTG
Cuts at: 0 145 2031
Size: 145 1886
SanDI GG'GwC_CC
Cuts at: 0 1297 2031
Size: 1297 734
SapI GCTCTTCn'nnn_
Cuts at: 0 281 1805 1904 2031
Size: 281 1524 99 127
Fragments arranged by size:
1524 281 127 99
SfcI C'TryA_G
Cuts at: 0 682 719 1057 1118 2031
Size: 682 37 338 61 913
Fragments arranged by size:
913 682 338 61 37
SmaI CCC'GGG
Cuts at: 0 627 2031
Size: 627 1404
SmlI C'TyrA_G
Cuts at: 0 311 659 2031
Size: 311 348 1372
Fragments arranged by size:
1372 348 311
SphI G_CATG'C
Cuts at: 0 994 1287 2031
Size: 994 293 744
Fragments arranged by size:
994 744 293
SrfI GCCC'GGGC
Cuts at: 0 627 2031
Size: 627 1404
StuI AGG'CCT
Cuts at: 0 608 2031
Size: 608 1423
StyI C'CwwG_G
Cuts at: 0 603 1254 1766 2031
Size: 603 651 512 265
Fragments arranged by size:
651 603 512 265
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1267 2031
Size: 1267 764
TatI w'GTAC_w
Cuts at: 0 1009 1026 1041 1896 2031
Size: 1009 17 15 855 135
Fragments arranged by size:
1009 855 135 17 15
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 904 2031
Size: 904 1127
XmaI C'CCGG_G
Cuts at: 0 625 2031
Size: 625 1406
Enzymes that do cut:
AarI AccI AclI AcuI AflIII ApaI ApoI AvaI
BanII BbsI BbvCI BfrBI BglII BlpI Bme1580I BmgBI
BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspMI BsrDI BsrFI BsrGI BstAPI BstEII BstYI BstZ17I
BtgI BtsI DraIII EaeI EagI EarI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I MmeI MscI MslI
MspA1I NaeI NcoI NdeI NgoMIV NsiI NspI PciI
PflMI PmlI PpuMI PshAI PsiI PspOMI PstI PvuII
SanDI SapI SfcI SmaI SmlI SphI SrfI StuI
StyI TaqII TatI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AfeI AflII AgeI AhdI AleI AlfI
AloI AlwNI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BcgI BciVI BclI BglI BmrI
BmtI BsaI BsaWI BsiWI BspEI BspHI BsrBI BssHII
BssSI BstBI BstXI Bsu36I BtgZI ClaI CspCI DraI
DrdI EciI EcoICRI EcoNI EcoRV FseI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI NarI NheI
NotI NruI PacI PasI PfoI PmeI PpiI PspXI
PsrI PvuI RsrII SacI SacII SalI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SspI
SwaI TstI Tth111I XbaI XhoI XmnI ZraI