Restriction map for
pF1KB6242
(Linear) (Six-base) MAPSORT of: KIBB6242.seq Check: 3257 from: 1 to: 2061
KIBB6242.orf
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1083 2061
Size: 1083 978
AccI GT'mk_AC
Cuts at: 0 1051 1957 2061
Size: 1051 906 104
Fragments arranged by size:
1051 906 104
Acc65I G'GTAC_C
Cuts at: 0 1754 2061
Size: 1754 307
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1246 1453 2045 2061
Size: 1246 207 592 16
Fragments arranged by size:
1246 592 207 16
AhdI GACnn_n'nnGTC
Cuts at: 0 1953 2061
Size: 1953 108
AleI CACnn'nnGTG
Cuts at: 0 402 2061
Size: 402 1659
AlwNI CAG_nnn'CTG
Cuts at: 0 459 2061
Size: 459 1602
ApaLI G'TGCA_C
Cuts at: 0 1480 2061
Size: 1480 581
ApoI r'AATT_y
Cuts at: 0 174 668 947 1290 1831 1964 2061
Size: 174 494 279 343 541 133 97
Fragments arranged by size:
541 494 343 279 174 133 97
BamHI G'GATC_C
Cuts at: 0 15 132 151 2061
Size: 15 117 19 1910
Fragments arranged by size:
1910 117 19 15
BanI G'GyrC_C
Cuts at: 0 33 1754 2061
Size: 33 1721 307
Fragments arranged by size:
1721 307 33
BanII G_rGCy'C
Cuts at: 0 581 1780 2061
Size: 581 1199 281
Fragments arranged by size:
1199 581 281
BbsI GAAGACnn'nnnn_
Cuts at: 0 841 995 1396 1913 2061
Size: 841 154 401 517 148
Fragments arranged by size:
841 517 401 154 148
BciVI GTATCCnnnnn_n'
Cuts at: 0 784 2061
Size: 784 1277
BclI T'GATC_A
Cuts at: 0 1175 2061
Size: 1175 886
BglII A'GATC_T
Cuts at: 0 1491 2061
Size: 1491 570
Bme1580I G_kGCm'C
Cuts at: 0 1484 2061
Size: 1484 577
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 561 1799 2061
Size: 561 1238 262
Fragments arranged by size:
1238 561 262
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 1445 1501 2061
Size: 496 949 56 560
Fragments arranged by size:
949 560 496 56
Bpu10I CC'TnA_GC
Cuts at: 0 314 2061
Size: 314 1747
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1928 2061
Size: 1928 133
BsaI GGTCTCn'nnnn_
Cuts at: 0 463 502 1565 2061
Size: 463 39 1063 496
Fragments arranged by size:
1063 496 463 39
BsaBI GATnn'nnATC
Cuts at: 0 1145 2061
Size: 1145 916
BsaHI Gr'CG_yC
Cuts at: 0 1712 2061
Size: 1712 349
BsaWI w'CCGG_w
Cuts at: 0 1541 2061
Size: 1541 520
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 17 47 1075 1105 2061
Size: 17 30 1028 30 956
Fragments arranged by size:
1028 956 30 30 17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 252 457 493 1567 1819 2005 2061
Size: 252 205 36 1074 252 186 56
Fragments arranged by size:
1074 252 252 205 186 56 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1508 1772 2061
Size: 1508 264 289
Fragments arranged by size:
1508 289 264
BsiHKAI G_wGCw'C
Cuts at: 0 384 581 1484 1765 2061
Size: 384 197 903 281 296
Fragments arranged by size:
903 384 296 281 197
Bsp1286I G_dGCh'C
Cuts at: 0 384 581 1484 1765 1780 2061
Size: 384 197 903 281 15 281
Fragments arranged by size:
903 384 281 281 197 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1083 2061
Size: 1083 978
BsrDI GCAATG_nn'
Cuts at: 0 266 2061
Size: 266 1795
BsrFI r'CCGG_y
Cuts at: 0 884 1811 2061
Size: 884 927 250
Fragments arranged by size:
927 884 250
BsrGI T'GTAC_A
Cuts at: 0 1205 2061
Size: 1205 856
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1123 2061
Size: 1123 938
BstEII G'GTnAC_C
Cuts at: 0 880 2061
Size: 880 1181
BstYI r'GATC_y
Cuts at: 0 15 132 151 1491 2061
Size: 15 117 19 1340 570
Fragments arranged by size:
1340 570 117 19 15
BtgI C'CryG_G
Cuts at: 0 29 1631 2061
Size: 29 1602 430
Fragments arranged by size:
1602 430 29
BtsI GCAGTG_nn'
Cuts at: 0 1926 2061
Size: 1926 135
ClaI AT'CG_AT
Cuts at: 0 213 2061
Size: 213 1848
DraI TTT'AAA
Cuts at: 0 558 945 1254 2061
Size: 558 387 309 807
Fragments arranged by size:
807 558 387 309
EaeI y'GGCC_r
Cuts at: 0 323 461 2061
Size: 323 138 1600
Fragments arranged by size:
1600 323 138
EarI CTCTTCn'nnn_
Cuts at: 0 1982 2003 2061
Size: 1982 21 58
Fragments arranged by size:
1982 58 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1552 2061
Size: 1552 509
EcoICRI GAG'CTC
Cuts at: 0 579 2061
Size: 579 1482
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 1246 1445 1453 1501 2045 2061
Size: 496 750 199 8 48 544 16
Fragments arranged by size:
750 544 496 199 48 16 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 817 2061
Size: 817 1244
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 829 1331 2061
Size: 829 502 730
Fragments arranged by size:
829 730 502
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 288 320 555 587 616 648 1852 1884
Size: 288 32 235 32 29 32 1204 32
Cuts at: 1884 1939 1971 2061
Size: 55 32 90
Fragments arranged by size:
1204 288 235 90 55 32 32 32
32 32 29
HincII GTy'rAC
Cuts at: 0 190 2061
Size: 190 1871
KpnI G_GTAC'C
Cuts at: 0 1758 2061
Size: 1758 303
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 242 572 703 2061
Size: 242 330 131 1358
Fragments arranged by size:
1358 330 242 131
MscI TGG'CCA
Cuts at: 0 325 463 2061
Size: 325 138 1598
Fragments arranged by size:
1598 325 138
MslI CAynn'nnrTG
Cuts at: 0 287 402 998 1004 1154 2061
Size: 287 115 596 6 150 907
Fragments arranged by size:
907 596 287 150 115 6
MspA1I CmG'CkG
Cuts at: 0 1521 2061
Size: 1521 540
NaeI GCC'GGC
Cuts at: 0 1813 2061
Size: 1813 248
NgoMIV G'CCGG_C
Cuts at: 0 1811 2061
Size: 1811 250
PflMI CCAn_nnn'nTGG
Cuts at: 0 459 784 1239 2061
Size: 459 325 455 822
Fragments arranged by size:
822 459 455 325
PfoI T'CCnGG_A
Cuts at: 0 253 2061
Size: 253 1808
PshAI GACnn'nnGTC
Cuts at: 0 198 491 714 2061
Size: 198 293 223 1347
Fragments arranged by size:
1347 293 223 198
PstI C_TGCA'G
Cuts at: 0 1038 1921 2061
Size: 1038 883 140
Fragments arranged by size:
1038 883 140
PvuII CAG'CTG
Cuts at: 0 1521 2061
Size: 1521 540
RsrII CG'GwC_CG
Cuts at: 0 1539 2061
Size: 1539 522
SacI G_AGCT'C
Cuts at: 0 581 2061
Size: 581 1480
SfcI C'TryA_G
Cuts at: 0 84 514 1034 1917 2061
Size: 84 430 520 883 144
Fragments arranged by size:
883 520 430 144 84
SmlI C'TyrA_G
Cuts at: 0 1943 2061
Size: 1943 118
StuI AGG'CCT
Cuts at: 0 448 2061
Size: 448 1613
StyI C'CwwG_G
Cuts at: 0 128 778 818 1566 2061
Size: 128 650 40 748 495
Fragments arranged by size:
748 650 495 128 40
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 414 593 598 1589 2061
Size: 414 179 5 991 472
Fragments arranged by size:
991 472 414 179 5
TatI w'GTAC_w
Cuts at: 0 1205 1939 2061
Size: 1205 734 122
Fragments arranged by size:
1205 734 122
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 17 49 602 634 1612 1644 2061
Size: 17 32 553 32 978 32 417
Fragments arranged by size:
978 553 417 32 32 32 17
Tth111I GACn'n_nGTC
Cuts at: 0 122 1699 2061
Size: 122 1577 362
Fragments arranged by size:
1577 362 122
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 471 2061
Size: 471 1590
Enzymes that do cut:
AarI AccI Acc65I AcuI AhdI AleI AlwNI ApaLI
ApoI BamHI BanI BanII BbsI BciVI BclI BglII
Bme1580I BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI
BsaWI BsaXI BseRI BsgI BsiHKAI Bsp1286I BspMI BsrDI
BsrFI BsrGI BstAPI BstEII BstYI BtgI BtsI ClaI
DraI EaeI EarI EciI EcoICRI Eco57MI EcoNI FalI
Hin4I HincII KpnI MmeI MscI MslI MspA1I NaeI
NgoMIV PflMI PfoI PshAI PstI PvuII RsrII SacI
SfcI SmlI StuI StyI TaqII TatI TstI Tth111I
XcmI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI AlfI AloI
ApaI AscI AseI AsiSI AvaI AvrII BaeI BbeI
BbvCI BcgI BfrBI BglI BlpI BmgBI BmrI BmtI
BsaAI BseYI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BssHII BssSI BstBI BstXI BstZ17I Bsu36I BtgZI
CspCI DraIII DrdI EagI EcoO109I EcoRI EcoRV FseI
FspI FspAI HaeII HindIII HpaI KasI MfeI MluI
NarI NcoI NdeI NheI NotI NruI NsiI NspI
PacI PasI PciI PmeI PmlI PpiI PpuMI PsiI
PspOMI PspXI PsrI PvuI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI XbaI XhoI
XmaI XmnI ZraI