Restriction map for
pF1KB6243
(Linear) (Six-base) MAPSORT of: KIBB6243.seq Check: 8211 from: 1 to: 2259
KIBB6243.orf
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
Acc65I G'GTAC_C
Cuts at: 0 416 2259
Size: 416 1843
AclI AA'CG_TT
Cuts at: 0 682 2259
Size: 682 1577
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 719 2021 2096 2259
Size: 719 1302 75 163
Fragments arranged by size:
1302 719 163 75
AhdI GACnn_n'nnGTC
Cuts at: 0 2184 2259
Size: 2184 75
AlwNI CAG_nnn'CTG
Cuts at: 0 1042 1605 2044 2175 2259
Size: 1042 563 439 131 84
Fragments arranged by size:
1042 563 439 131 84
ApaI G_GGCC'C
Cuts at: 0 2006 2259
Size: 2006 253
ApaLI G'TGCA_C
Cuts at: 0 577 2259
Size: 577 1682
ApoI r'AATT_y
Cuts at: 0 1056 1128 1236 1763 2259
Size: 1056 72 108 527 496
Fragments arranged by size:
1056 527 496 108 72
AvaI C'yCGr_G
Cuts at: 0 2050 2259
Size: 2050 209
AvrII C'CTAG_G
Cuts at: 0 1344 2259
Size: 1344 915
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 408 441 1793 1826 2259
Size: 408 33 1352 33 433
Fragments arranged by size:
1352 433 408 33 33
BamHI G'GATC_C
Cuts at: 0 563 2259
Size: 563 1696
BanI G'GyrC_C
Cuts at: 0 105 416 1470 1615 1732 2259
Size: 105 311 1054 145 117 527
Fragments arranged by size:
1054 527 311 145 117 105
BanII G_rGCy'C
Cuts at: 0 1583 1837 1987 2006 2259
Size: 1583 254 150 19 253
Fragments arranged by size:
1583 254 253 150 19
BbsI GAAGACnn'nnnn_
Cuts at: 0 490 2259
Size: 490 1769
BbvCI CC'TCA_GC
Cuts at: 0 1084 1311 2259
Size: 1084 227 948
Fragments arranged by size:
1084 948 227
BciVI GTATCCnnnnn_n'
Cuts at: 0 984 2259
Size: 984 1275
BclI T'GATC_A
Cuts at: 0 1868 2259
Size: 1868 391
Bme1580I G_kGCm'C
Cuts at: 0 581 2006 2259
Size: 581 1425 253
Fragments arranged by size:
1425 581 253
BmgBI CAC'GTC
Cuts at: 0 609 2259
Size: 609 1650
BmrI ACTGGGnnnn_n'
Cuts at: 0 869 2259
Size: 869 1390
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1385 1958 2152 2259
Size: 1385 573 194 107
Fragments arranged by size:
1385 573 194 107
Bpu10I CC'TnA_GC
Cuts at: 0 79 1084 1311 2259
Size: 79 1005 227 948
Fragments arranged by size:
1005 948 227 79
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1041 2259
Size: 1041 1218
BsaAI yAC'GTr
Cuts at: 0 570 2259
Size: 570 1689
BsaBI GATnn'nnATC
Cuts at: 0 995 1873 2259
Size: 995 878 386
Fragments arranged by size:
995 878 386
BsaWI w'CCGG_w
Cuts at: 0 387 2259
Size: 387 1872
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1647 1677 1728 1758 2259
Size: 1647 30 51 30 501
Fragments arranged by size:
1647 501 51 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 509 1316 1950 2067 2239 2242 2259
Size: 509 807 634 117 172 3 17
Fragments arranged by size:
807 634 509 172 117 17 3
BseYI C'CCAG_C
Cuts at: 0 1292 1358 1507 2005 2019 2259
Size: 1292 66 149 498 14 240
Fragments arranged by size:
1292 498 240 149 66 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 631 2259
Size: 301 330 1628
Fragments arranged by size:
1628 330 301
BsiHKAI G_wGCw'C
Cuts at: 0 581 1837 2259
Size: 581 1256 422
Fragments arranged by size:
1256 581 422
BsmI GAATG_Cn'
Cuts at: 0 628 2259
Size: 628 1631
Bsp1286I G_dGCh'C
Cuts at: 0 581 1583 1837 1987 2006 2259
Size: 581 1002 254 150 19 253
Fragments arranged by size:
1002 581 254 253 150 19
BspEI T'CCGG_A
Cuts at: 0 387 2259
Size: 387 1872
BspHI T'CATG_A
Cuts at: 0 255 936 2259
Size: 255 681 1323
Fragments arranged by size:
1323 681 255
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 476 1314 2259
Size: 476 838 945
Fragments arranged by size:
945 838 476
BsrBI CCG'CTC
Cuts at: 0 1932 2259
Size: 1932 327
BsrDI GCAATG_nn'
Cuts at: 0 269 2259
Size: 269 1990
BsrGI T'GTAC_A
Cuts at: 0 641 2259
Size: 641 1618
BstBI TT'CG_AA
Cuts at: 0 750 2259
Size: 750 1509
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1849 2259
Size: 1849 410
BstYI r'GATC_y
Cuts at: 0 563 1155 2259
Size: 563 592 1104
Fragments arranged by size:
1104 592 563
Bsu36I CC'TnA_GG
Cuts at: 0 559 2259
Size: 559 1700
BtgI C'CryG_G
Cuts at: 0 1592 2141 2259
Size: 1592 549 118
Fragments arranged by size:
1592 549 118
BtsI GCAGTG_nn'
Cuts at: 0 1538 1802 2259
Size: 1538 264 457
Fragments arranged by size:
1538 457 264
DraIII CAC_nnn'GTG
Cuts at: 0 730 2259
Size: 730 1529
EarI CTCTTCn'nnn_
Cuts at: 0 227 1259 1880 2040 2259
Size: 227 1032 621 160 219
Fragments arranged by size:
1032 621 227 219 160
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1211 1319 2259
Size: 1211 108 940
Fragments arranged by size:
1211 940 108
EcoICRI GAG'CTC
Cuts at: 0 1835 2259
Size: 1835 424
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 719 1385 1958 2021 2096 2152 2259
Size: 719 666 573 63 75 56 107
Fragments arranged by size:
719 666 573 107 75 63 56
EcoO109I rG'GnC_Cy
Cuts at: 0 159 1080 1348 2259
Size: 159 921 268 911
Fragments arranged by size:
921 911 268 159
EcoRI G'AATT_C
Cuts at: 0 1128 2259
Size: 1128 1131
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 559 1471 2259
Size: 559 912 788
Fragments arranged by size:
912 788 559
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1189 1221 1921 1953 2146 2170 2178 2202
Size: 1189 32 700 32 193 24 8 24
Cuts at: 2202 2259
Size: 57
Fragments arranged by size:
1189 700 193 57 32 32 24 24
8
KpnI G_GTAC'C
Cuts at: 0 420 2259
Size: 420 1839
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 359 2259
Size: 359 1900
MslI CAynn'nnrTG
Cuts at: 0 337 600 777 851 1896 2146 2259
Size: 337 263 177 74 1045 250 113
Fragments arranged by size:
1045 337 263 250 177 113 74
MspA1I CmG'CkG
Cuts at: 0 1528 1543 2259
Size: 1528 15 716
Fragments arranged by size:
1528 716 15
NcoI C'CATG_G
Cuts at: 0 1592 2141 2259
Size: 1592 549 118
Fragments arranged by size:
1592 549 118
NspI r_CATG'y
Cuts at: 0 1895 1913 2259
Size: 1895 18 346
Fragments arranged by size:
1895 346 18
PasI CC'CwG_GG
Cuts at: 0 270 1071 1611 1828 2259
Size: 270 801 540 217 431
Fragments arranged by size:
801 540 431 270 217
PflMI CCAn_nnn'nTGG
Cuts at: 0 2175 2259
Size: 2175 84
PmlI CAC'GTG
Cuts at: 0 570 2259
Size: 570 1689
PpuMI rG'GwC_Cy
Cuts at: 0 159 1080 1348 2259
Size: 159 921 268 911
Fragments arranged by size:
921 911 268 159
PspOMI G'GGCC_C
Cuts at: 0 2002 2259
Size: 2002 257
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1697 1729 2259
Size: 1697 32 530
Fragments arranged by size:
1697 530 32
PstI C_TGCA'G
Cuts at: 0 377 709 1685 1809 2015 2219 2259
Size: 377 332 976 124 206 204 40
Fragments arranged by size:
976 377 332 206 204 124 40
PvuII CAG'CTG
Cuts at: 0 1543 2259
Size: 1543 716
SacI G_AGCT'C
Cuts at: 0 1837 2259
Size: 1837 422
ScaI AGT'ACT
Cuts at: 0 1572 2259
Size: 1572 687
SfcI C'TryA_G
Cuts at: 0 373 450 705 1681 1805 2011 2215 2259
Size: 373 77 255 976 124 206 204 44
Fragments arranged by size:
976 373 255 206 204 124 77 44
SmaI CCC'GGG
Cuts at: 0 2052 2259
Size: 2052 207
SmlI C'TyrA_G
Cuts at: 0 1020 2259
Size: 1020 1239
SphI G_CATG'C
Cuts at: 0 1913 2259
Size: 1913 346
SspI AAT'ATT
Cuts at: 0 351 2259
Size: 351 1908
StyI C'CwwG_G
Cuts at: 0 1344 1592 2141 2259
Size: 1344 248 549 118
Fragments arranged by size:
1344 549 248 118
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 351 1603 2259
Size: 351 1252 656
Fragments arranged by size:
1252 656 351
TatI w'GTAC_w
Cuts at: 0 500 641 1263 1570 2259
Size: 500 141 622 307 689
Fragments arranged by size:
689 622 500 307 141
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1647 1679 1728 1760 2259
Size: 1647 32 49 32 499
Fragments arranged by size:
1647 499 49 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1695 1996 2259
Size: 1695 301 263
Fragments arranged by size:
1695 301 263
XmaI C'CCGG_G
Cuts at: 0 2050 2259
Size: 2050 209
XmnI GAAnn'nnTTC
Cuts at: 0 628 895 1763 2259
Size: 628 267 868 496
Fragments arranged by size:
868 628 496 267
Enzymes that do cut:
Acc65I AclI AcuI AhdI AlwNI ApaI ApaLI ApoI
AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI
BciVI BclI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI
BsaAI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrGI
BstBI BstXI BstYI Bsu36I BtgI BtsI DraIII EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI Hin4I KpnI
MmeI MslI MspA1I NcoI NspI PasI PflMI PmlI
PpuMI PspOMI PsrI PstI PvuII SacI ScaI SfcI
SmaI SmlI SphI SspI StyI TaqII TatI TstI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AfeI AflII AflIII AgeI AleI
AlfI AloI AscI AseI AsiSI BbeI BcgI BfrBI
BglI BglII BlpI BmtI BplI BsaI BsaHI BsiEI
BsiWI BsmBI BsrFI BssHII BssSI BstAPI BstEII BstZ17I
BtgZI ClaI CspCI DraI DrdI EaeI EagI EcoNI
EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI
KasI MfeI MluI MscI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PfoI PmeI
PpiI PshAI PsiI PspXI PvuI RsrII SacII SalI
SanDI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SnaBI SpeI SrfI StuI SwaI Tth111I XbaI XhoI
ZraI