Restriction map for
pF1KB6245
(Linear) (Six-base) MAPSORT of: KIBB6245.seq Check: 8275 from: 1 to: 2259
KIBB6245.orf
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 204 219 1407 1428 2259
Size: 204 15 1188 21 831
Fragments arranged by size:
1188 831 204 21 15
AccI GT'mk_AC
Cuts at: 0 1503 1772 2259
Size: 1503 269 487
Fragments arranged by size:
1503 487 269
Acc65I G'GTAC_C
Cuts at: 0 1276 1964 2259
Size: 1276 688 295
Fragments arranged by size:
1276 688 295
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 824 969 1598 2259
Size: 824 145 629 661
Fragments arranged by size:
824 661 629 145
AflIII A'CryG_T
Cuts at: 0 1767 2259
Size: 1767 492
AgeI A'CCGG_T
Cuts at: 0 472 2259
Size: 472 1787
AlwNI CAG_nnn'CTG
Cuts at: 0 390 423 2259
Size: 390 33 1836
Fragments arranged by size:
1836 390 33
ApaI G_GGCC'C
Cuts at: 0 431 813 2259
Size: 431 382 1446
Fragments arranged by size:
1446 431 382
ApoI r'AATT_y
Cuts at: 0 1198 1917 2049 2259
Size: 1198 719 132 210
Fragments arranged by size:
1198 719 210 132
AvaI C'yCGr_G
Cuts at: 0 16 367 455 511 649 661 2259
Size: 16 351 88 56 138 12 1598
Fragments arranged by size:
1598 351 138 88 56 16 12
AvrII C'CTAG_G
Cuts at: 0 1693 2259
Size: 1693 566
BamHI G'GATC_C
Cuts at: 0 776 815 1046 1494 2259
Size: 776 39 231 448 765
Fragments arranged by size:
776 765 448 231 39
BanI G'GyrC_C
Cuts at: 0 76 352 745 1276 1964 2259
Size: 76 276 393 531 688 295
Fragments arranged by size:
688 531 393 295 276 76
BanII G_rGCy'C
Cuts at: 0 107 431 689 813 834 1435 2259
Size: 107 324 258 124 21 601 824
Fragments arranged by size:
824 601 324 258 124 107 21
BbeI G_GCGC'C
Cuts at: 0 356 2259
Size: 356 1903
BbsI GAAGACnn'nnnn_
Cuts at: 0 1964 2157 2259
Size: 1964 193 102
Fragments arranged by size:
1964 193 102
BbvCI CC'TCA_GC
Cuts at: 0 29 2135 2259
Size: 29 2106 124
Fragments arranged by size:
2106 124 29
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1914 1948 2259
Size: 1914 34 311
Fragments arranged by size:
1914 311 34
BclI T'GATC_A
Cuts at: 0 632 2259
Size: 632 1627
Bme1580I G_kGCm'C
Cuts at: 0 79 431 813 2259
Size: 79 352 382 1446
Fragments arranged by size:
1446 382 352 79
BmrI ACTGGGnnnn_n'
Cuts at: 0 1550 2259
Size: 1550 709
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 96 2259
Size: 96 2163
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 345 471 577 925 1452 2259
Size: 345 126 106 348 527 807
Fragments arranged by size:
807 527 348 345 126 106
Bpu10I CC'TnA_GC
Cuts at: 0 29 1478 2135 2248 2259
Size: 29 1449 657 113 11
Fragments arranged by size:
1449 657 113 29 11
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2010 2259
Size: 2010 249
BsaAI yAC'GTr
Cuts at: 0 1337 2259
Size: 1337 922
BsaHI Gr'CG_yC
Cuts at: 0 178 353 2259
Size: 178 175 1906
Fragments arranged by size:
1906 178 175
BsaWI w'CCGG_w
Cuts at: 0 472 742 2259
Size: 472 270 1517
Fragments arranged by size:
1517 472 270
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 676 706 2169 2199 2259
Size: 676 30 1463 30 60
Fragments arranged by size:
1463 676 60 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 580 649 2242 2259
Size: 580 69 1593 17
Fragments arranged by size:
1593 580 69 17
BseYI C'CCAG_C
Cuts at: 0 423 430 1077 1354 1459 2259
Size: 423 7 647 277 105 800
Fragments arranged by size:
800 647 423 277 105 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 403 2259
Size: 403 1856
BsiEI CG_ry'CG
Cuts at: 0 71 2259
Size: 71 2188
BsiHKAI G_wGCw'C
Cuts at: 0 834 1734 2249 2259
Size: 834 900 515 10
Fragments arranged by size:
900 834 515 10
BsmI GAATG_Cn'
Cuts at: 0 1572 1945 2038 2259
Size: 1572 373 93 221
Fragments arranged by size:
1572 373 221 93
BsmBI CGTCTCn'nnnn_
Cuts at: 0 122 1470 2259
Size: 122 1348 789
Fragments arranged by size:
1348 789 122
Bsp1286I G_dGCh'C
Cuts at: 0 79 107 431 689 813 834 1435 1734
Size: 79 28 324 258 124 21 601 299
Cuts at: 1734 2249 2259
Size: 515 10
Fragments arranged by size:
601 515 324 299 258 124 79 28
21 10
BspHI T'CATG_A
Cuts at: 0 572 2259
Size: 572 1687
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 204 219 326 1407 1428 2259
Size: 204 15 107 1081 21 831
Fragments arranged by size:
1081 831 204 107 21 15
BsrBI CCG'CTC
Cuts at: 0 52 942 1328 2259
Size: 52 890 386 931
Fragments arranged by size:
931 890 386 52
BsrFI r'CCGG_y
Cuts at: 0 66 472 516 2259
Size: 66 406 44 1743
Fragments arranged by size:
1743 406 66 44
BstAPI GCAn_nnn'nTGC
Cuts at: 0 142 234 381 1654 2259
Size: 142 92 147 1273 605
Fragments arranged by size:
1273 605 147 142 92
BstXI CCAn_nnnn'nTGG
Cuts at: 0 702 2259
Size: 702 1557
BstYI r'GATC_y
Cuts at: 0 776 815 1046 1494 2259
Size: 776 39 231 448 765
Fragments arranged by size:
776 765 448 231 39
Bsu36I CC'TnA_GG
Cuts at: 0 251 772 2259
Size: 251 521 1487
Fragments arranged by size:
1487 521 251
BtgI C'CryG_G
Cuts at: 0 98 194 2259
Size: 98 96 2065
Fragments arranged by size:
2065 98 96
BtsI GCAGTG_nn'
Cuts at: 0 179 2259
Size: 179 2080
DraI TTT'AAA
Cuts at: 0 1997 2259
Size: 1997 262
DraIII CAC_nnn'GTG
Cuts at: 0 381 1593 2044 2259
Size: 381 1212 451 215
Fragments arranged by size:
1212 451 381 215
EaeI y'GGCC_r
Cuts at: 0 1791 2259
Size: 1791 468
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 44 577 824 2259
Size: 44 533 247 1435
Fragments arranged by size:
1435 533 247 44
EcoICRI GAG'CTC
Cuts at: 0 832 2259
Size: 832 1427
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 345 471 577 824 925 969 1452 1598
Size: 345 126 106 247 101 44 483 146
Cuts at: 1598 2259
Size: 661
Fragments arranged by size:
661 483 345 247 146 126 106 101
44
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1226 2259
Size: 1226 1033
EcoO109I rG'GnC_Cy
Cuts at: 0 427 451 508 809 864 2259
Size: 427 24 57 301 55 1395
Fragments arranged by size:
1395 427 301 57 55 24
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1847 2259
Size: 1847 412
FseI GG_CCGG'CC
Cuts at: 0 520 2259
Size: 520 1739
HaeII r_GCGC'y
Cuts at: 0 356 397 1628 2259
Size: 356 41 1231 631
Fragments arranged by size:
1231 631 356 41
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 449 481 676 708 2169 2201 2259
Size: 449 32 195 32 1461 32 58
Fragments arranged by size:
1461 449 195 58 32 32 32
KasI G'GCGC_C
Cuts at: 0 352 2259
Size: 352 1907
KpnI G_GTAC'C
Cuts at: 0 1280 1968 2259
Size: 1280 688 291
Fragments arranged by size:
1280 688 291
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 482 1710 2259
Size: 482 1228 549
Fragments arranged by size:
1228 549 482
MscI TGG'CCA
Cuts at: 0 1793 2259
Size: 1793 466
MslI CAynn'nnrTG
Cuts at: 0 1766 2259
Size: 1766 493
MspA1I CmG'CkG
Cuts at: 0 63 196 274 1410 1569 2139 2259
Size: 63 133 78 1136 159 570 120
Fragments arranged by size:
1136 570 159 133 120 78 63
NaeI GCC'GGC
Cuts at: 0 518 2259
Size: 518 1741
NarI GG'CG_CC
Cuts at: 0 353 2259
Size: 353 1906
NcoI C'CATG_G
Cuts at: 0 98 2259
Size: 98 2161
NgoMIV G'CCGG_C
Cuts at: 0 516 2259
Size: 516 1743
NspI r_CATG'y
Cuts at: 0 403 1771 1886 2259
Size: 403 1368 115 373
Fragments arranged by size:
1368 403 373 115
PasI CC'CwG_GG
Cuts at: 0 805 2259
Size: 805 1454
PciI A'CATG_T
Cuts at: 0 1767 2259
Size: 1767 492
PfoI T'CCnGG_A
Cuts at: 0 1490 2259
Size: 1490 769
PpuMI rG'GwC_Cy
Cuts at: 0 864 2259
Size: 864 1395
PshAI GACnn'nnGTC
Cuts at: 0 862 2259
Size: 862 1397
PspOMI G'GGCC_C
Cuts at: 0 427 809 2259
Size: 427 382 1450
Fragments arranged by size:
1450 427 382
PspXI vC'TCGA_Gb
Cuts at: 0 455 661 2259
Size: 455 206 1598
Fragments arranged by size:
1598 455 206
PstI C_TGCA'G
Cuts at: 0 215 332 673 1425 2259
Size: 215 117 341 752 834
Fragments arranged by size:
834 752 341 215 117
PvuII CAG'CTG
Cuts at: 0 1410 1569 2139 2259
Size: 1410 159 570 120
Fragments arranged by size:
1410 570 159 120
SacI G_AGCT'C
Cuts at: 0 834 2259
Size: 834 1425
SacII CC_GC'GG
Cuts at: 0 197 2259
Size: 197 2062
ScaI AGT'ACT
Cuts at: 0 1653 2259
Size: 1653 606
SfcI C'TryA_G
Cuts at: 0 211 328 669 1360 1421 2259
Size: 211 117 341 691 61 838
Fragments arranged by size:
838 691 341 211 117 61
SfoI GGC'GCC
Cuts at: 0 354 2259
Size: 354 1905
SmaI CCC'GGG
Cuts at: 0 513 2259
Size: 513 1746
SmlI C'TyrA_G
Cuts at: 0 455 661 2025 2259
Size: 455 206 1364 234
Fragments arranged by size:
1364 455 234 206
SnaBI TAC'GTA
Cuts at: 0 1337 2259
Size: 1337 922
StyI C'CwwG_G
Cuts at: 0 98 149 860 1693 1788 2153 2259
Size: 98 51 711 833 95 365 106
Fragments arranged by size:
833 711 365 106 98 95 51
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1778 2259
Size: 1778 481
TatI w'GTAC_w
Cuts at: 0 1651 1879 2259
Size: 1651 228 380
Fragments arranged by size:
1651 380 228
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1388 1409 1420 1441 2259
Size: 1388 21 11 21 818
Fragments arranged by size:
1388 818 21 21 11
Tth111I GACn'n_nGTC
Cuts at: 0 342 2259
Size: 342 1917
XbaI T'CTAG_A
Cuts at: 0 1617 2259
Size: 1617 642
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 147 1537 2259
Size: 147 1390 722
Fragments arranged by size:
1390 722 147
XhoI C'TCGA_G
Cuts at: 0 455 661 2259
Size: 455 206 1598
Fragments arranged by size:
1598 455 206
XmaI C'CCGG_G
Cuts at: 0 511 2259
Size: 511 1748
XmnI GAAnn'nnTTC
Cuts at: 0 2164 2259
Size: 2164 95
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AgeI AlwNI ApaI
ApoI AvaI AvrII BamHI BanI BanII BbeI BbsI
BbvCI BcgI BclI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI
BsrFI BstAPI BstXI BstYI Bsu36I BtgI BtsI DraI
DraIII EaeI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
FseI HaeII Hin4I KasI KpnI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PfoI PpuMI PshAI PspOMI PspXI PstI PvuII SacI
SacII ScaI SfcI SfoI SmaI SmlI SnaBI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AhdI AleI AlfI AloI
ApaLI AscI AseI AsiSI BaeI BciVI BfrBI BglI
BglII BlpI BmgBI BmtI BsaI BsaBI BsiWI BspEI
BsrDI BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgZI
ClaI CspCI DrdI EagI EarI EcoRI EcoRV FspI
FspAI HincII HindIII HpaI MfeI MluI NdeI NheI
NotI NruI NsiI PacI PflMI PmeI PmlI PpiI
PsiI PsrI PvuI RsrII SalI SanDI SapI SbfI
SexAI SfiI SgfI SgrAI SpeI SphI SrfI SspI
StuI SwaI ZraI