Restriction map for
pF1KB6249
(Linear) (Six-base) MAPSORT of: KIBB6249.seq Check: 3017 from: 1 to: 2268
KIBB6249.orf
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AccI GT'mk_AC
Cuts at: 0 608 2268
Size: 608 1660
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1339 1768 1993 2150 2268
Size: 1339 429 225 157 118
Fragments arranged by size:
1339 429 225 157 118
AflIII A'CryG_T
Cuts at: 0 31 2268
Size: 31 2237
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1354 2268
Size: 1354 914
AlwNI CAG_nnn'CTG
Cuts at: 0 564 1056 1317 2268
Size: 564 492 261 951
Fragments arranged by size:
951 564 492 261
ApaI G_GGCC'C
Cuts at: 0 887 2268
Size: 887 1381
ApoI r'AATT_y
Cuts at: 0 1407 2268
Size: 1407 861
AvaI C'yCGr_G
Cuts at: 0 227 1733 2268
Size: 227 1506 535
Fragments arranged by size:
1506 535 227
AvrII C'CTAG_G
Cuts at: 0 498 2268
Size: 498 1770
BamHI G'GATC_C
Cuts at: 0 870 2268
Size: 870 1398
BanI G'GyrC_C
Cuts at: 0 173 258 358 625 848 958 2268
Size: 173 85 100 267 223 110 1310
Fragments arranged by size:
1310 267 223 173 110 100 85
BanII G_rGCy'C
Cuts at: 0 149 887 1250 1568 1651 1961 2268
Size: 149 738 363 318 83 310 307
Fragments arranged by size:
738 363 318 310 307 149 83
BbeI G_GCGC'C
Cuts at: 0 262 2268
Size: 262 2006
BbsI GAAGACnn'nnnn_
Cuts at: 0 746 1927 2268
Size: 746 1181 341
Fragments arranged by size:
1181 746 341
BbvCI CC'TCA_GC
Cuts at: 0 1276 2268
Size: 1276 992
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 525 559 1078 1112 2268
Size: 525 34 519 34 1156
Fragments arranged by size:
1156 525 519 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1246 2268
Size: 1246 1022
BglI GCCn_nnn'nGGC
Cuts at: 0 1655 2268
Size: 1655 613
BglII A'GATC_T
Cuts at: 0 306 1199 2268
Size: 306 893 1069
Fragments arranged by size:
1069 893 306
BlpI GC'TnA_GC
Cuts at: 0 148 809 2268
Size: 148 661 1459
Fragments arranged by size:
1459 661 148
Bme1580I G_kGCm'C
Cuts at: 0 868 887 961 2268
Size: 868 19 74 1307
Fragments arranged by size:
1307 868 74 19
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 325 2268
Size: 325 1943
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 601 636 838 1501 1560 2235 2268
Size: 199 402 35 202 663 59 675 33
Fragments arranged by size:
675 663 402 202 199 59 35 33
Bpu10I CC'TnA_GC
Cuts at: 0 1276 2096 2268
Size: 1276 820 172
Fragments arranged by size:
1276 820 172
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1420 2268
Size: 1420 848
BsaI GGTCTCn'nnnn_
Cuts at: 0 1730 2268
Size: 1730 538
BsaAI yAC'GTr
Cuts at: 0 878 2268
Size: 878 1390
BsaBI GATnn'nnATC
Cuts at: 0 124 187 2268
Size: 124 63 2081
Fragments arranged by size:
2081 124 63
BsaHI Gr'CG_yC
Cuts at: 0 259 2268
Size: 259 2009
BsaWI w'CCGG_w
Cuts at: 0 311 2268
Size: 311 1957
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 317 1057 1363 1659 1705 2268
Size: 317 740 306 296 46 563
Fragments arranged by size:
740 563 317 306 296 46
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 706 2130 2268
Size: 706 1424 138
Fragments arranged by size:
1424 706 138
BsiHKAI G_wGCw'C
Cuts at: 0 149 1568 1961 2246 2268
Size: 149 1419 393 285 22
Fragments arranged by size:
1419 393 285 149 22
BsmI GAATG_Cn'
Cuts at: 0 693 2034 2268
Size: 693 1341 234
Fragments arranged by size:
1341 693 234
Bsp1286I G_dGCh'C
Cuts at: 0 149 868 887 961 1250 1568 1651 1961
Size: 149 719 19 74 289 318 83 310
Cuts at: 1961 2246 2268
Size: 285 22
Fragments arranged by size:
719 318 310 289 285 149 83 74
22 19
BspEI T'CCGG_A
Cuts at: 0 311 2268
Size: 311 1957
BspHI T'CATG_A
Cuts at: 0 191 408 2268
Size: 191 217 1860
Fragments arranged by size:
1860 217 191
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 278 2268
Size: 278 1990
BsrBI CCG'CTC
Cuts at: 0 168 2268
Size: 168 2100
BsrDI GCAATG_nn'
Cuts at: 0 383 2268
Size: 383 1885
BsrFI r'CCGG_y
Cuts at: 0 137 1334 2268
Size: 137 1197 934
Fragments arranged by size:
1197 934 137
BsrGI T'GTAC_A
Cuts at: 0 1709 2268
Size: 1709 559
BstAPI GCAn_nnn'nTGC
Cuts at: 0 533 684 837 2268
Size: 533 151 153 1431
Fragments arranged by size:
1431 533 153 151
BstEII G'GTnAC_C
Cuts at: 0 8 2268
Size: 8 2260
BstXI CCAn_nnnn'nTGG
Cuts at: 0 219 516 2268
Size: 219 297 1752
Fragments arranged by size:
1752 297 219
BstYI r'GATC_y
Cuts at: 0 182 306 432 870 1152 1199 2268
Size: 182 124 126 438 282 47 1069
Fragments arranged by size:
1069 438 282 182 126 124 47
Bsu36I CC'TnA_GG
Cuts at: 0 239 2268
Size: 239 2029
BtgI C'CryG_G
Cuts at: 0 284 1207 1412 1652 1995 2075 2268
Size: 284 923 205 240 343 80 193
Fragments arranged by size:
923 343 284 240 205 193 80
BtsI GCAGTG_nn'
Cuts at: 0 139 694 2268
Size: 139 555 1574
Fragments arranged by size:
1574 555 139
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1977 2012 2268
Size: 1977 35 256
Fragments arranged by size:
1977 256 35
DraIII CAC_nnn'GTG
Cuts at: 0 1106 2268
Size: 1106 1162
EaeI y'GGCC_r
Cuts at: 0 281 2268
Size: 281 1987
EarI CTCTTCn'nnn_
Cuts at: 0 706 1883 2104 2234 2268
Size: 706 1177 221 130 34
Fragments arranged by size:
1177 706 221 130 34
EcoICRI GAG'CTC
Cuts at: 0 147 1566 1959 2268
Size: 147 1419 393 309
Fragments arranged by size:
1419 393 309 147
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 601 636 838 1339 1501 1560 1768
Size: 199 402 35 202 501 162 59 208
Cuts at: 1768 1993 2150 2235 2268
Size: 225 157 85 33
Fragments arranged by size:
501 402 225 208 202 199 162 157
85 59 35 33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2257 2268
Size: 2257 11
EcoO109I rG'GnC_Cy
Cuts at: 0 38 551 903 2268
Size: 38 513 352 1365
Fragments arranged by size:
1365 513 352 38
EcoRI G'AATT_C
Cuts at: 0 1407 2268
Size: 1407 861
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 76 2268
Size: 76 2192
HaeII r_GCGC'y
Cuts at: 0 60 262 1867 2268
Size: 60 202 1605 401
Fragments arranged by size:
1605 401 202 60
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 373 405 1599 1631 2268
Size: 373 32 1194 32 637
Fragments arranged by size:
1194 637 373 32 32
HincII GTy'rAC
Cuts at: 0 609 2268
Size: 609 1659
KasI G'GCGC_C
Cuts at: 0 258 2268
Size: 258 2010
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1044 1427 2108 2268
Size: 1044 383 681 160
Fragments arranged by size:
1044 681 383 160
MscI TGG'CCA
Cuts at: 0 283 2268
Size: 283 1985
MslI CAynn'nnrTG
Cuts at: 0 212 2268
Size: 212 2056
MspA1I CmG'CkG
Cuts at: 0 1232 1275 1388 2058 2249 2268
Size: 1232 43 113 670 191 19
Fragments arranged by size:
1232 670 191 113 43 19
NaeI GCC'GGC
Cuts at: 0 139 2268
Size: 139 2129
NarI GG'CG_CC
Cuts at: 0 259 2268
Size: 259 2009
NcoI C'CATG_G
Cuts at: 0 284 1412 1652 2268
Size: 284 1128 240 616
Fragments arranged by size:
1128 616 284 240
NgoMIV G'CCGG_C
Cuts at: 0 137 2268
Size: 137 2131
NspI r_CATG'y
Cuts at: 0 35 2268
Size: 35 2233
PciI A'CATG_T
Cuts at: 0 31 2268
Size: 31 2237
PflMI CCAn_nnn'nTGG
Cuts at: 0 1143 2268
Size: 1143 1125
PpuMI rG'GwC_Cy
Cuts at: 0 551 2268
Size: 551 1717
PshAI GACnn'nnGTC
Cuts at: 0 1927 2268
Size: 1927 341
PspOMI G'GGCC_C
Cuts at: 0 883 2268
Size: 883 1385
PstI C_TGCA'G
Cuts at: 0 461 560 1001 1064 1343 1622 1940 2268
Size: 461 99 441 63 279 279 318 328
Fragments arranged by size:
461 441 328 318 279 279 99 63
PvuII CAG'CTG
Cuts at: 0 1232 1275 1388 2058 2249 2268
Size: 1232 43 113 670 191 19
Fragments arranged by size:
1232 670 191 113 43 19
SacI G_AGCT'C
Cuts at: 0 149 1568 1961 2268
Size: 149 1419 393 307
Fragments arranged by size:
1419 393 307 149
SalI G'TCGA_C
Cuts at: 0 607 2268
Size: 607 1661
SanDI GG'GwC_CC
Cuts at: 0 551 2268
Size: 551 1717
SapI GCTCTTCn'nnn_
Cuts at: 0 2234 2268
Size: 2234 34
ScaI AGT'ACT
Cuts at: 0 292 2268
Size: 292 1976
SfcI C'TryA_G
Cuts at: 0 457 556 997 1060 1339 1618 1936 2268
Size: 457 99 441 63 279 279 318 332
Fragments arranged by size:
457 441 332 318 279 279 99 63
SfoI GGC'GCC
Cuts at: 0 260 2268
Size: 260 2008
SmlI C'TyrA_G
Cuts at: 0 1435 1733 2268
Size: 1435 298 535
Fragments arranged by size:
1435 535 298
StuI AGG'CCT
Cuts at: 0 2263 2268
Size: 2263 5
StyI C'CwwG_G
Cuts at: 0 60 284 297 498 509 1412 1652 1880
Size: 60 224 13 201 11 903 240 228
Cuts at: 1880 2268
Size: 388
Fragments arranged by size:
903 388 240 228 224 201 60 13
11
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1639 2268
Size: 1639 629
TatI w'GTAC_w
Cuts at: 0 290 593 1487 1709 2268
Size: 290 303 894 222 559
Fragments arranged by size:
894 559 303 290 222
XhoI C'TCGA_G
Cuts at: 0 1733 2268
Size: 1733 535
Enzymes that do cut:
AccI AcuI AflIII AlfI AlwNI ApaI ApoI AvaI
AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BglI BglII BlpI Bme1580I BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BsgI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtsI CspCI DraIII EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI HaeII Hin4I HincII KasI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PflMI PpuMI PshAI PspOMI PstI PvuII SacI
SalI SanDI SapI ScaI SfcI SfoI SmlI StuI
StyI TaqII TatI XhoI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApaLI AscI AseI AsiSI BaeI BclI
BfrBI BmgBI BmrI BmtI BsaXI BseYI BsiEI BsiWI
BsmBI BssHII BssSI BstBI BstZ17I BtgZI ClaI DraI
DrdI EagI EciI EcoRV FseI FspI FspAI HindIII
HpaI KpnI MfeI MluI NdeI NheI NotI NruI
NsiI PacI PasI PfoI PmeI PmlI PpiI PsiI
PspXI PsrI PvuI RsrII SacII SbfI SexAI SfiI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TstI Tth111I XbaI XcmI XmaI XmnI ZraI