Restriction map for
pF1KB6254
(Linear) (Six-base) MAPSORT of: KIBB6254.seq Check: 7083 from: 1 to: 2049
KIBB6254.orf
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 692 735 1485 2049
Size: 692 43 750 564
Fragments arranged by size:
750 692 564 43
Acc65I G'GTAC_C
Cuts at: 0 1589 2049
Size: 1589 460
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 763 2049
Size: 763 1286
AgeI A'CCGG_T
Cuts at: 0 233 2049
Size: 233 1816
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 730 2049
Size: 730 1319
AlwNI CAG_nnn'CTG
Cuts at: 0 845 1399 1477 1570 2049
Size: 845 554 78 93 479
Fragments arranged by size:
845 554 479 93 78
ApaI G_GGCC'C
Cuts at: 0 111 1013 1281 2049
Size: 111 902 268 768
Fragments arranged by size:
902 768 268 111
ApoI r'AATT_y
Cuts at: 0 958 2049
Size: 958 1091
AvaI C'yCGr_G
Cuts at: 0 67 173 241 250 831 995 1004 1084
Size: 67 106 68 9 581 164 9 80
Cuts at: 1084 1281 1895 2049
Size: 197 614 154
Fragments arranged by size:
614 581 197 164 154 106 80 68
67 9 9
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 870 903 2049
Size: 870 33 1146
Fragments arranged by size:
1146 870 33
BanI G'GyrC_C
Cuts at: 0 246 466 680 1164 1208 1589 1845 2049
Size: 246 220 214 484 44 381 256 204
Fragments arranged by size:
484 381 256 246 220 214 204 44
BanII G_rGCy'C
Cuts at: 0 111 450 512 1004 1013 1281 1313 1419
Size: 111 339 62 492 9 268 32 106
Cuts at: 1419 1448 2049
Size: 29 601
Fragments arranged by size:
601 492 339 268 111 106 62 32
29 9
BbeI G_GCGC'C
Cuts at: 0 1212 2049
Size: 1212 837
BbsI GAAGACnn'nnnn_
Cuts at: 0 1983 2049
Size: 1983 66
BbvCI CC'TCA_GC
Cuts at: 0 361 2049
Size: 361 1688
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 45 79 2049
Size: 45 34 1970
Fragments arranged by size:
1970 45 34
BglI GCCn_nnn'nGGC
Cuts at: 0 16 1008 1795 1959 2049
Size: 16 992 787 164 90
Fragments arranged by size:
992 787 164 90 16
BlpI GC'TnA_GC
Cuts at: 0 1035 2049
Size: 1035 1014
Bme1580I G_kGCm'C
Cuts at: 0 68 111 251 1013 1167 1281 1748 1763
Size: 68 43 140 762 154 114 467 15
Cuts at: 1763 1791 1891 2049
Size: 28 100 158
Fragments arranged by size:
762 467 158 154 140 114 100 68
43 28 15
BmgBI CAC'GTC
Cuts at: 0 495 2049
Size: 495 1554
BmrI ACTGGGnnnn_n'
Cuts at: 0 652 2049
Size: 652 1397
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1050 2049
Size: 1050 999
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 298 1867 1936 2049
Size: 59 239 1569 69 113
Fragments arranged by size:
1569 239 113 69 59
Bpu10I CC'TnA_GC
Cuts at: 0 286 361 1312 1548 2049
Size: 286 75 951 236 501
Fragments arranged by size:
951 501 286 236 75
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1334 1663 1777 2049
Size: 1334 329 114 272
Fragments arranged by size:
1334 329 272 114
BsaI GGTCTCn'nnnn_
Cuts at: 0 77 1040 1500 1572 2049
Size: 77 963 460 72 477
Fragments arranged by size:
963 477 460 77 72
BsaAI yAC'GTr
Cuts at: 0 1998 2049
Size: 1998 51
BsaHI Gr'CG_yC
Cuts at: 0 1209 2049
Size: 1209 840
BsaWI w'CCGG_w
Cuts at: 0 233 566 977 2049
Size: 233 333 411 1072
Fragments arranged by size:
1072 411 333 233
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 81 111 631 661 1037 1067 2049
Size: 81 30 520 30 376 30 982
Fragments arranged by size:
982 520 376 81 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 55 130 1129 1198 1213 1524 1870 2049
Size: 55 75 999 69 15 311 346 179
Fragments arranged by size:
999 346 311 179 75 69 55 15
BseYI C'CCAG_C
Cuts at: 0 321 441 600 2049
Size: 321 120 159 1449
Fragments arranged by size:
1449 321 159 120
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 119 1075 2049
Size: 119 956 974
Fragments arranged by size:
974 956 119
BsiEI CG_ry'CG
Cuts at: 0 11 1735 2049
Size: 11 1724 314
Fragments arranged by size:
1724 314 11
BsiHKAI G_wGCw'C
Cuts at: 0 512 1867 2049
Size: 512 1355 182
Fragments arranged by size:
1355 512 182
BsmBI CGTCTCn'nnnn_
Cuts at: 0 84 651 2049
Size: 84 567 1398
Fragments arranged by size:
1398 567 84
Bsp1286I G_dGCh'C
Cuts at: 0 68 111 251 450 512 1004 1013 1167
Size: 68 43 140 199 62 492 9 154
Cuts at: 1167 1281 1313 1419 1448 1748 1763 1791 1867
Size: 114 32 106 29 300 15 28 76
Cuts at: 1867 1891 2049
Size: 24 158
Fragments arranged by size:
492 300 199 158 154 140 114 106
76 68 62 43 32 29 28 24
15 9
BspEI T'CCGG_A
Cuts at: 0 566 977 2049
Size: 566 411 1072
Fragments arranged by size:
1072 566 411
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 678 692 735 1485 2049
Size: 678 14 43 750 564
Fragments arranged by size:
750 678 564 43 14
BsrBI CCG'CTC
Cuts at: 0 105 1203 1239 1265 2049
Size: 105 1098 36 26 784
Fragments arranged by size:
1098 784 105 36 26
BsrFI r'CCGG_y
Cuts at: 0 152 233 1188 2049
Size: 152 81 955 861
Fragments arranged by size:
955 861 152 81
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1399 2049
Size: 1399 650
BstYI r'GATC_y
Cuts at: 0 858 980 1875 2049
Size: 858 122 895 174
Fragments arranged by size:
895 858 174 122
Bsu36I CC'TnA_GG
Cuts at: 0 412 676 1670 1850 2049
Size: 412 264 994 180 199
Fragments arranged by size:
994 412 264 199 180
BtgI C'CryG_G
Cuts at: 0 122 1483 2049
Size: 122 1361 566
Fragments arranged by size:
1361 566 122
BtsI GCAGTG_nn'
Cuts at: 0 102 1889 2049
Size: 102 1787 160
Fragments arranged by size:
1787 160 102
DrdI GACnn_nn'nnGTC
Cuts at: 0 562 1078 2049
Size: 562 516 971
Fragments arranged by size:
971 562 516
EaeI y'GGCC_r
Cuts at: 0 8 125 1486 1679 1691 1718 1732 2049
Size: 8 117 1361 193 12 27 14 317
Fragments arranged by size:
1361 317 193 117 27 14 12 8
EagI C'GGCC_G
Cuts at: 0 8 1732 2049
Size: 8 1724 317
Fragments arranged by size:
1724 317 8
EarI CTCTTCn'nnn_
Cuts at: 0 549 590 1091 1528 2049
Size: 549 41 501 437 521
Fragments arranged by size:
549 521 501 437 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 610 2049
Size: 610 1439
EcoICRI GAG'CTC
Cuts at: 0 510 2049
Size: 510 1539
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 59 298 763 1867 1936 2049
Size: 59 239 465 1104 69 113
Fragments arranged by size:
1104 465 239 113 69 59
EcoNI CCTnn'n_nnAGG
Cuts at: 0 115 1145 1669 1681 2049
Size: 115 1030 524 12 368
Fragments arranged by size:
1030 524 368 115 12
EcoO109I rG'GnC_Cy
Cuts at: 0 108 134 282 417 555 606 810 1009
Size: 108 26 148 135 138 51 204 199
Cuts at: 1009 1277 1278 1552 1638 1674 1944 2049
Size: 268 1 274 86 36 270 105
Fragments arranged by size:
274 270 268 204 199 148 138 135
108 105 86 51 36 26 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1465 2049
Size: 1465 584
HaeII r_GCGC'y
Cuts at: 0 159 1212 2049
Size: 159 1053 837
Fragments arranged by size:
1053 837 159
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 499 531 825 857 1849 1881 2049
Size: 499 32 294 32 992 32 168
Fragments arranged by size:
992 499 294 168 32 32 32
HincII GTy'rAC
Cuts at: 0 950 2049
Size: 950 1099
KasI G'GCGC_C
Cuts at: 0 1208 2049
Size: 1208 841
KpnI G_GTAC'C
Cuts at: 0 1593 2049
Size: 1593 456
MfeI C'AATT_G
Cuts at: 0 300 2049
Size: 300 1749
MscI TGG'CCA
Cuts at: 0 127 1693 1720 2049
Size: 127 1566 27 329
Fragments arranged by size:
1566 329 127 27
MslI CAynn'nnrTG
Cuts at: 0 1756 2049
Size: 1756 293
MspA1I CmG'CkG
Cuts at: 0 267 375 405 1039 1227 1405 1629 1659
Size: 267 108 30 634 188 178 224 30
Cuts at: 1659 1716 2049
Size: 57 333
Fragments arranged by size:
634 333 267 224 188 178 108 57
30 30
NaeI GCC'GGC
Cuts at: 0 1190 2049
Size: 1190 859
NarI GG'CG_CC
Cuts at: 0 1209 2049
Size: 1209 840
NgoMIV G'CCGG_C
Cuts at: 0 1188 2049
Size: 1188 861
PasI CC'CwG_GG
Cuts at: 0 471 804 1141 2049
Size: 471 333 337 908
Fragments arranged by size:
908 471 337 333
PfoI T'CCnGG_A
Cuts at: 0 226 867 2049
Size: 226 641 1182
Fragments arranged by size:
1182 641 226
PpuMI rG'GwC_Cy
Cuts at: 0 134 417 555 606 810 1638 1674 2049
Size: 134 283 138 51 204 828 36 375
Fragments arranged by size:
828 375 283 204 138 134 51 36
PspOMI G'GGCC_C
Cuts at: 0 107 1009 1277 2049
Size: 107 902 268 772
Fragments arranged by size:
902 772 268 107
PstI C_TGCA'G
Cuts at: 0 689 719 855 1404 1658 2049
Size: 689 30 136 549 254 391
Fragments arranged by size:
689 549 391 254 136 30
PvuII CAG'CTG
Cuts at: 0 267 375 405 1227 1629 1716 2049
Size: 267 108 30 822 402 87 333
Fragments arranged by size:
822 402 333 267 108 87 30
RsrII CG'GwC_CG
Cuts at: 0 1907 2049
Size: 1907 142
SacI G_AGCT'C
Cuts at: 0 512 2049
Size: 512 1537
SanDI GG'GwC_CC
Cuts at: 0 134 2049
Size: 134 1915
SapI GCTCTTCn'nnn_
Cuts at: 0 590 1091 2049
Size: 590 501 958
Fragments arranged by size:
958 590 501
SbfI CC_TGCA'GG
Cuts at: 0 689 2049
Size: 689 1360
SfcI C'TryA_G
Cuts at: 0 685 715 851 1400 1654 2049
Size: 685 30 136 549 254 395
Fragments arranged by size:
685 549 395 254 136 30
SfoI GGC'GCC
Cuts at: 0 1210 2049
Size: 1210 839
SmaI CCC'GGG
Cuts at: 0 69 175 997 1006 1086 1897 2049
Size: 69 106 822 9 80 811 152
Fragments arranged by size:
822 811 152 106 80 69 9
SmlI C'TyrA_G
Cuts at: 0 1349 1642 1792 2049
Size: 1349 293 150 257
Fragments arranged by size:
1349 293 257 150
StyI C'CwwG_G
Cuts at: 0 326 2049
Size: 326 1723
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 226 2049
Size: 226 1823
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 81 113 1037 1069 2049
Size: 81 32 924 32 980
Fragments arranged by size:
980 924 81 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1570 2049
Size: 1570 479
XbaI T'CTAG_A
Cuts at: 0 1821 2049
Size: 1821 228
XmaI C'CCGG_G
Cuts at: 0 67 173 995 1004 1084 1895 2049
Size: 67 106 822 9 80 811 154
Fragments arranged by size:
822 811 154 106 80 67 9
XmnI GAAnn'nnTTC
Cuts at: 0 166 2049
Size: 166 1883
Enzymes that do cut:
AarI Acc65I AcuI AgeI AlfI AlwNI ApaI ApoI
AvaI BaeI BanI BanII BbeI BbsI BbvCI BcgI
BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI
BsrFI BstAPI BstYI Bsu36I BtgI BtsI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
HaeII Hin4I HincII KasI KpnI MfeI MscI MslI
MspA1I NaeI NarI NgoMIV PasI PfoI PpuMI PspOMI
PstI PvuII RsrII SacI SanDI SapI SbfI SfcI
SfoI SmaI SmlI StyI TaqII TstI Tth111I XbaI
XmaI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AhdI AleI
AloI ApaLI AscI AseI AsiSI AvrII BamHI BciVI
BclI BfrBI BglII BmtI BsaBI BsiWI BsmI BspHI
BsrDI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I
BtgZI ClaI CspCI DraI DraIII EcoRI EcoRV FseI
FspI FspAI HindIII HpaI MluI MmeI NcoI NdeI
NheI NotI NruI NsiI NspI PacI PciI PflMI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
SacII SalI ScaI SexAI SfiI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI StuI SwaI TatI XcmI
XhoI ZraI