Restriction map for
pF1KB6264
(Linear) (Six-base) MAPSORT of: KIBB6264.seq Check: 1762 from: 1 to: 2307
KIBB6264.orf
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 194 2307
Size: 194 2113
AccI GT'mk_AC
Cuts at: 0 690 2307
Size: 690 1617
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 69 233 588 922 987 2307
Size: 69 164 355 334 65 1320
Fragments arranged by size:
1320 355 334 164 69 65
AflIII A'CryG_T
Cuts at: 0 951 2153 2307
Size: 951 1202 154
Fragments arranged by size:
1202 951 154
AgeI A'CCGG_T
Cuts at: 0 1430 2307
Size: 1430 877
AleI CACnn'nnGTG
Cuts at: 0 964 1661 2307
Size: 964 697 646
Fragments arranged by size:
964 697 646
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 191 223 2307
Size: 191 32 2084
Fragments arranged by size:
2084 191 32
AlwNI CAG_nnn'CTG
Cuts at: 0 905 1157 1829 1863 2081 2307
Size: 905 252 672 34 218 226
Fragments arranged by size:
905 672 252 226 218 34
ApoI r'AATT_y
Cuts at: 0 400 514 617 701 869 1121 1205 1373
Size: 400 114 103 84 168 252 84 168
Cuts at: 1373 1625 1709 1793 2045 2129 2307
Size: 252 84 84 252 84 178
Fragments arranged by size:
400 252 252 252 178 168 168 114
103 84 84 84 84 84
BanI G'GyrC_C
Cuts at: 0 125 2307
Size: 125 2182
BbsI GAAGACnn'nnnn_
Cuts at: 0 850 993 2011 2307
Size: 850 143 1018 296
Fragments arranged by size:
1018 850 296 143
BbvCI CC'TCA_GC
Cuts at: 0 33 2307
Size: 33 2274
BciVI GTATCCnnnnn_n'
Cuts at: 0 357 380 2307
Size: 357 23 1927
Fragments arranged by size:
1927 357 23
BfrBI ATG'CAT
Cuts at: 0 479 777 1235 2307
Size: 479 298 458 1072
Fragments arranged by size:
1072 479 458 298
BglII A'GATC_T
Cuts at: 0 681 2307
Size: 681 1626
Bme1580I G_kGCm'C
Cuts at: 0 258 2307
Size: 258 2049
BmgBI CAC'GTC
Cuts at: 0 277 2307
Size: 277 2030
BmrI ACTGGGnnnn_n'
Cuts at: 0 1559 2307
Size: 1559 748
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 484 652 2307
Size: 484 168 1655
Fragments arranged by size:
1655 484 168
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 190 731 899 1151 1319 1655 1823
Size: 29 161 541 168 252 168 336 168
Cuts at: 1823 2075 2159 2243 2307
Size: 252 84 84 64
Fragments arranged by size:
541 336 252 252 168 168 168 161
84 84 64 29
Bpu10I CC'TnA_GC
Cuts at: 0 33 2307
Size: 33 2274
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 754 2307
Size: 754 1553
BsaI GGTCTCn'nnnn_
Cuts at: 0 166 2307
Size: 166 2141
BsaAI yAC'GTr
Cuts at: 0 2154 2307
Size: 2154 153
BsaWI w'CCGG_w
Cuts at: 0 625 1430 1969 2307
Size: 625 805 539 338
Fragments arranged by size:
805 625 539 338
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 918 2094 2307
Size: 918 1176 213
Fragments arranged by size:
1176 918 213
BsmI GAATG_Cn'
Cuts at: 0 397 649 733 748 826 1153 1420 1573
Size: 397 252 84 15 78 327 267 153
Cuts at: 1573 1657 1825 2307
Size: 84 168 482
Fragments arranged by size:
482 397 327 267 252 168 153 84
84 78 15
Bsp1286I G_dGCh'C
Cuts at: 0 258 2307
Size: 258 2049
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 194 1925 2307
Size: 194 1731 382
Fragments arranged by size:
1731 382 194
BsrDI GCAATG_nn'
Cuts at: 0 570 2307
Size: 570 1737
BsrFI r'CCGG_y
Cuts at: 0 1430 2307
Size: 1430 877
BsrGI T'GTAC_A
Cuts at: 0 501 2307
Size: 501 1806
BstXI CCAn_nnnn'nTGG
Cuts at: 0 11 1663 2307
Size: 11 1652 644
Fragments arranged by size:
1652 644 11
BstYI r'GATC_y
Cuts at: 0 681 802 2307
Size: 681 121 1505
Fragments arranged by size:
1505 681 121
BstZ17I GTA'TAC
Cuts at: 0 691 2307
Size: 691 1616
BtgI C'CryG_G
Cuts at: 0 250 2307
Size: 250 2057
BtsI GCAGTG_nn'
Cuts at: 0 256 1158 1830 2082 2307
Size: 256 902 672 252 225
Fragments arranged by size:
902 672 256 252 225
ClaI AT'CG_AT
Cuts at: 0 101 2307
Size: 101 2206
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 552 587 1392 1427 1560 1595 2307
Size: 552 35 805 35 133 35 712
Fragments arranged by size:
805 712 552 133 35 35 35
DraI TTT'AAA
Cuts at: 0 558 2307
Size: 558 1749
DraIII CAC_nnn'GTG
Cuts at: 0 1745 2307
Size: 1745 562
EarI CTCTTCn'nnn_
Cuts at: 0 607 1746 2307
Size: 607 1139 561
Fragments arranged by size:
1139 607 561
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 69 190 233 588 731 899 922
Size: 29 40 121 43 355 143 168 23
Cuts at: 922 987 1151 1319 1655 1823 2075 2159 2243
Size: 65 164 168 336 168 252 84 84
Cuts at: 2243 2307
Size: 64
Fragments arranged by size:
355 336 252 168 168 168 164 143
121 84 84 65 64 43 40 29
23
EcoRI G'AATT_C
Cuts at: 0 617 869 1121 1205 1373 1793 2045 2129
Size: 617 252 252 84 168 420 252 84
Cuts at: 2129 2307
Size: 178
Fragments arranged by size:
617 420 252 252 252 178 168 84
84
EcoRV GAT'ATC
Cuts at: 0 202 2307
Size: 202 2105
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1287 1791 2307
Size: 1287 504 516
Fragments arranged by size:
1287 516 504
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 6 38 261 293 794 826 1098 1130
Size: 6 32 223 32 501 32 272 32
Cuts at: 1130 1266 1298 1518 1550 1745 1777 2022 2054
Size: 136 32 220 32 195 32 245 32
Cuts at: 2054 2307
Size: 253
Fragments arranged by size:
501 272 253 245 223 220 195 136
32 32 32 32 32 32 32 32
6
HindIII A'AGCT_T
Cuts at: 0 383 2307
Size: 383 1924
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 1040 2307
Size: 212 828 1267
Fragments arranged by size:
1267 828 212
MslI CAynn'nnrTG
Cuts at: 0 894 950 964 1316 1661 2307
Size: 894 56 14 352 345 646
Fragments arranged by size:
894 646 352 345 56 14
NcoI C'CATG_G
Cuts at: 0 250 2307
Size: 250 2057
NdeI CA'TA_TG
Cuts at: 0 539 891 1063 2307
Size: 539 352 172 1244
Fragments arranged by size:
1244 539 352 172
NsiI A_TGCA'T
Cuts at: 0 481 779 1237 2307
Size: 481 298 458 1070
Fragments arranged by size:
1070 481 458 298
PshAI GACnn'nnGTC
Cuts at: 0 2019 2307
Size: 2019 288
PsiI TTA'TAA
Cuts at: 0 338 674 2307
Size: 338 336 1633
Fragments arranged by size:
1633 338 336
PstI C_TGCA'G
Cuts at: 0 263 2307
Size: 263 2044
SapI GCTCTTCn'nnn_
Cuts at: 0 1746 2307
Size: 1746 561
SfcI C'TryA_G
Cuts at: 0 177 259 1092 2307
Size: 177 82 833 1215
Fragments arranged by size:
1215 833 177 82
SmlI C'TyrA_G
Cuts at: 0 769 2307
Size: 769 1538
StuI AGG'CCT
Cuts at: 0 832 1084 1504 1924 2176 2307
Size: 832 252 420 420 252 131
Fragments arranged by size:
832 420 420 252 252 131
StyI C'CwwG_G
Cuts at: 0 250 2307
Size: 250 2057
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 433 1525 2307
Size: 433 1092 782
Fragments arranged by size:
1092 782 433
TatI w'GTAC_w
Cuts at: 0 438 501 1066 2307
Size: 438 63 565 1241
Fragments arranged by size:
1241 565 438 63
XmnI GAAnn'nnTTC
Cuts at: 0 664 1000 1168 1420 1672 2092 2260 2307
Size: 664 336 168 252 252 420 168 47
Fragments arranged by size:
664 420 336 252 252 168 168 47
Enzymes that do cut:
AarI AccI AcuI AflIII AgeI AleI AloI AlwNI
ApoI BanI BbsI BbvCI BciVI BfrBI BglII Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaWI BsgI BsmI Bsp1286I BspMI BsrDI BsrFI BsrGI
BstXI BstYI BstZ17I BtgI BtsI ClaI CspCI DraI
DraIII EarI Eco57MI EcoRI EcoRV FalI Hin4I HindIII
MmeI MslI NcoI NdeI NsiI PshAI PsiI PstI
SapI SfcI SmlI StuI StyI TaqII TatI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AhdI AlfI ApaI
ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BanII BbeI BcgI BclI BglI BlpI BmtI BsaBI
BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI BsiWI BsmBI
BspEI BspHI BsrBI BssHII BssSI BstAPI BstBI BstEII
Bsu36I BtgZI DrdI EaeI EagI EciI EcoICRI EcoNI
EcoO109I FseI FspI FspAI HaeII HincII HpaI KasI
KpnI MfeI MluI MscI MspA1I NaeI NarI NgoMIV
NheI NotI NruI NspI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PspOMI PspXI PsrI
PvuI PvuII RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI
XcmI XhoI XmaI ZraI