Restriction map for
pF1KB6272
(Linear) (Six-base) MAPSORT of: KIBB6272.seq Check: 7497 from: 1 to: 2319
KIBB6272.orf
With 182 enzymes: SgfI *
October 7, 2009 14:20 ..
AclI AA'CG_TT
Cuts at: 0 1089 2319
Size: 1089 1230
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 836 2319
Size: 836 1483
AhdI GACnn_n'nnGTC
Cuts at: 0 561 2319
Size: 561 1758
AleI CACnn'nnGTG
Cuts at: 0 794 974 2319
Size: 794 180 1345
Fragments arranged by size:
1345 794 180
AlwNI CAG_nnn'CTG
Cuts at: 0 1067 2319
Size: 1067 1252
ApaI G_GGCC'C
Cuts at: 0 1525 2319
Size: 1525 794
AvaI C'yCGr_G
Cuts at: 0 168 2030 2319
Size: 168 1862 289
Fragments arranged by size:
1862 289 168
BanI G'GyrC_C
Cuts at: 0 33 2319
Size: 33 2286
BanII G_rGCy'C
Cuts at: 0 628 991 1525 1609 2078 2319
Size: 628 363 534 84 469 241
Fragments arranged by size:
628 534 469 363 241 84
BbsI GAAGACnn'nnnn_
Cuts at: 0 464 1126 2319
Size: 464 662 1193
Fragments arranged by size:
1193 662 464
BbvCI CC'TCA_GC
Cuts at: 0 1113 2319
Size: 1113 1206
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1274 1308 2319
Size: 1274 34 1011
Fragments arranged by size:
1274 1011 34
BfrBI ATG'CAT
Cuts at: 0 309 1560 2319
Size: 309 1251 759
Fragments arranged by size:
1251 759 309
BglII A'GATC_T
Cuts at: 0 1948 2319
Size: 1948 371
Bme1580I G_kGCm'C
Cuts at: 0 1525 2319
Size: 1525 794
BmrI ACTGGGnnnn_n'
Cuts at: 0 738 2319
Size: 738 1581
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 162 821 978 1286 1289 2188 2319
Size: 37 125 659 157 308 3 899 131
Fragments arranged by size:
899 659 308 157 131 125 37 3
Bpu10I CC'TnA_GC
Cuts at: 0 471 1113 1608 2319
Size: 471 642 495 711
Fragments arranged by size:
711 642 495 471
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2086 2319
Size: 2086 233
BsaBI GATnn'nnATC
Cuts at: 0 965 2319
Size: 965 1354
BsaWI w'CCGG_w
Cuts at: 0 1505 1722 2319
Size: 1505 217 597
Fragments arranged by size:
1505 597 217
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 184 214 2319
Size: 184 30 2105
Fragments arranged by size:
2105 184 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 31 981 1101 1349 1815 2319
Size: 31 950 120 248 466 504
Fragments arranged by size:
950 504 466 248 120 31
BseYI C'CCAG_C
Cuts at: 0 265 2319
Size: 265 2054
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 2110 2319
Size: 113 1997 209
Fragments arranged by size:
1997 209 113
BsiEI CG_ry'CG
Cuts at: 0 2082 2319
Size: 2082 237
BsiHKAI G_wGCw'C
Cuts at: 0 991 2078 2319
Size: 991 1087 241
Fragments arranged by size:
1087 991 241
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1424 2285 2319
Size: 1424 861 34
Fragments arranged by size:
1424 861 34
Bsp1286I G_dGCh'C
Cuts at: 0 628 991 1525 1609 2078 2319
Size: 628 363 534 84 469 241
Fragments arranged by size:
628 534 469 363 241 84
BspEI T'CCGG_A
Cuts at: 0 1505 2319
Size: 1505 814
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1880 2319
Size: 1880 439
BsrBI CCG'CTC
Cuts at: 0 2010 2319
Size: 2010 309
BsrFI r'CCGG_y
Cuts at: 0 1249 1621 2269 2319
Size: 1249 372 648 50
Fragments arranged by size:
1249 648 372 50
BstAPI GCAn_nnn'nTGC
Cuts at: 0 877 2319
Size: 877 1442
BstBI TT'CG_AA
Cuts at: 0 825 2319
Size: 825 1494
BstXI CCAn_nnnn'nTGG
Cuts at: 0 273 1162 2319
Size: 273 889 1157
Fragments arranged by size:
1157 889 273
BstYI r'GATC_y
Cuts at: 0 1948 2319
Size: 1948 371
Bsu36I CC'TnA_GG
Cuts at: 0 333 2319
Size: 333 1986
BtgI C'CryG_G
Cuts at: 0 800 1592 2319
Size: 800 792 727
Fragments arranged by size:
800 792 727
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 391 2319
Size: 391 1928
BtsI GCAGTG_nn'
Cuts at: 0 405 875 981 1523 2319
Size: 405 470 106 542 796
Fragments arranged by size:
796 542 470 405 106
DraI TTT'AAA
Cuts at: 0 120 2319
Size: 120 2199
EaeI y'GGCC_r
Cuts at: 0 963 2018 2079 2150 2267 2319
Size: 963 1055 61 71 117 52
Fragments arranged by size:
1055 963 117 71 61 52
EagI C'GGCC_G
Cuts at: 0 2079 2319
Size: 2079 240
EarI CTCTTCn'nnn_
Cuts at: 0 791 1044 1191 1709 2225 2319
Size: 791 253 147 518 516 94
Fragments arranged by size:
791 518 516 253 147 94
EcoICRI GAG'CTC
Cuts at: 0 989 2076 2319
Size: 989 1087 243
Fragments arranged by size:
1087 989 243
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 162 821 836 978 1286 1289 2188
Size: 37 125 659 15 142 308 3 899
Cuts at: 2188 2319
Size: 131
Fragments arranged by size:
899 659 308 142 131 125 37 15
3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1787 2140 2319
Size: 1787 353 179
Fragments arranged by size:
1787 353 179
EcoO109I rG'GnC_Cy
Cuts at: 0 185 476 555 1435 1661 1779 1971 2319
Size: 185 291 79 880 226 118 192 348
Fragments arranged by size:
880 348 291 226 192 185 118 79
EcoRV GAT'ATC
Cuts at: 0 1926 2319
Size: 1926 393
FseI GG_CCGG'CC
Cuts at: 0 2273 2319
Size: 2273 46
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 547 562 579 594 1287 1319 1634 1666
Size: 547 15 17 15 693 32 315 32
Cuts at: 1666 1831 1863 1870 1902 1915 1947 2224 2256
Size: 165 32 7 32 13 32 277 32
Cuts at: 2256 2319
Size: 63
Fragments arranged by size:
693 547 315 277 165 63 32 32
32 32 32 32 17 15 15 13
7
MscI TGG'CCA
Cuts at: 0 965 2020 2319
Size: 965 1055 299
Fragments arranged by size:
1055 965 299
MslI CAynn'nnrTG
Cuts at: 0 700 794 974 1082 1160 1534 2319
Size: 700 94 180 108 78 374 785
Fragments arranged by size:
785 700 374 180 108 94 78
MspA1I CmG'CkG
Cuts at: 0 1384 2319
Size: 1384 935
NaeI GCC'GGC
Cuts at: 0 1251 2271 2319
Size: 1251 1020 48
Fragments arranged by size:
1251 1020 48
NcoI C'CATG_G
Cuts at: 0 800 1592 2319
Size: 800 792 727
Fragments arranged by size:
800 792 727
NgoMIV G'CCGG_C
Cuts at: 0 1249 2269 2319
Size: 1249 1020 50
Fragments arranged by size:
1249 1020 50
NsiI A_TGCA'T
Cuts at: 0 311 1562 2319
Size: 311 1251 757
Fragments arranged by size:
1251 757 311
NspI r_CATG'y
Cuts at: 0 146 1560 1797 2319
Size: 146 1414 237 522
Fragments arranged by size:
1414 522 237 146
PflMI CCAn_nnn'nTGG
Cuts at: 0 1518 2148 2281 2319
Size: 1518 630 133 38
Fragments arranged by size:
1518 630 133 38
PfoI T'CCnGG_A
Cuts at: 0 178 508 2319
Size: 178 330 1811
Fragments arranged by size:
1811 330 178
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 151 183 214 246 2319
Size: 151 32 31 32 2073
Fragments arranged by size:
2073 151 32 32 31
PpuMI rG'GwC_Cy
Cuts at: 0 476 555 1435 1661 1779 1971 2319
Size: 476 79 880 226 118 192 348
Fragments arranged by size:
880 476 348 226 192 118 79
PspOMI G'GGCC_C
Cuts at: 0 1521 2319
Size: 1521 798
PspXI vC'TCGA_Gb
Cuts at: 0 168 2319
Size: 168 2151
PvuII CAG'CTG
Cuts at: 0 1384 2319
Size: 1384 935
SacI G_AGCT'C
Cuts at: 0 991 2078 2319
Size: 991 1087 241
Fragments arranged by size:
1087 991 241
SanDI GG'GwC_CC
Cuts at: 0 1661 2319
Size: 1661 658
ScaI AGT'ACT
Cuts at: 0 748 2319
Size: 748 1571
SexAI A'CCwGG_T
Cuts at: 0 735 1715 2319
Size: 735 980 604
Fragments arranged by size:
980 735 604
SfcI C'TryA_G
Cuts at: 0 1068 1807 2319
Size: 1068 739 512
Fragments arranged by size:
1068 739 512
SmlI C'TyrA_G
Cuts at: 0 168 2101 2319
Size: 168 1933 218
Fragments arranged by size:
1933 218 168
SphI G_CATG'C
Cuts at: 0 1797 2319
Size: 1797 522
StuI AGG'CCT
Cuts at: 0 783 1411 2319
Size: 783 628 908
Fragments arranged by size:
908 783 628
StyI C'CwwG_G
Cuts at: 0 188 282 558 684 800 982 1592 2264
Size: 188 94 276 126 116 182 610 672
Cuts at: 2264 2319
Size: 55
Fragments arranged by size:
672 610 276 188 182 126 116 94
55
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1585 2319
Size: 1585 734
TatI w'GTAC_w
Cuts at: 0 746 2319
Size: 746 1573
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 246 278 1653 1685 2319
Size: 246 32 1375 32 634
Fragments arranged by size:
1375 634 246 32 32
XbaI T'CTAG_A
Cuts at: 0 1393 2319
Size: 1393 926
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 186 1599 1672 2319
Size: 186 1413 73 647
Fragments arranged by size:
1413 647 186 73
XhoI C'TCGA_G
Cuts at: 0 168 2319
Size: 168 2151
XmnI GAAnn'nnTTC
Cuts at: 0 720 2319
Size: 720 1599
Enzymes that do cut:
AclI AcuI AhdI AleI AlwNI ApaI AvaI BanI
BanII BbsI BbvCI BcgI BfrBI BglII Bme1580I BmrI
BpmI Bpu10I BpuEI BsaBI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI
BsrFI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI EaeI EagI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRV FseI Hin4I MscI MslI MspA1I NaeI
NcoI NgoMIV NsiI NspI PflMI PfoI PpiI PpuMI
PspOMI PspXI PvuII SacI SanDI ScaI SexAI SfcI
SmlI SphI StuI StyI TaqII TatI TstI XbaI
XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AflIII AgeI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BciVI BclI BglI BlpI BmgBI
BmtI BplI BsaI BsaAI BsaHI BsiWI BsmI BspHI
BsrDI BsrGI BssHII BssSI BstEII BstZ17I ClaI CspCI
DraIII DrdI EciI EcoRI FalI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
NarI NdeI NheI NotI NruI PacI PasI PciI
PmeI PmlI PshAI PsiI PsrI PstI PvuI RsrII
SacII SalI SapI SbfI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI SwaI Tth111I XmaI
ZraI